• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.42 no.6
• /
• pp.759-765
• /
• 1997

The process of the senescence in Oryza glaberrima Steud. strains and Oryza sativa L. cultivars were examined in terms of SPAD value(chlorophyll content) and photosynthetic rate. SPAD values and photosynthetic rates of flag leaves for 10 genotypes of each species was measured at the 1, 3 and 5 weeks after heading. SPAD values and photosynthetic rates of O. glaberrima strains tended to decrease rapidly after heading. O. sativa cultivars showed slower decrease as compared to O. glaberrima strains, in particular during the period from 1 to 3 weeks after heading. Although there was no significant difference between the two species in the mean value of photosynthetic rate and SPAD value at 1 weeks after heading, O. glaberrima had lower values after 3 weeks after heading. There were significant positive correlation coefficients between the photosynthetic rate and the SPAD value at 1 and 3 weeks after heading for O. glaberrima strains, and at 1 and 5 weeks after heading for O. sativa cultivars. There were significant positive correlation between the decreasing rates of the photosynthetic rates and the decreasing rates of the SPAD values at the period from the 1 to 3 weeks after heading for both species. At the period from 3 to 5 weeks after heading, only O. glaberrima showed a significant correlation between two traits, indicating that rapid decrease in chlorophyll content would affect the photosynthesis in O. glaberrima.

• Korean Journal of Pharmacognosy
• /
• v.33 no.3 s.130
• /
• pp.173-176
• /
• 2002

In search for the plant-derived antioxidant compound, it was found that the EtOAc, BuOH extracts obtained from the leaves parts of Oryza sativa L. which exhibited a significant antioxidant activity from 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical cleavage. Activity-guided fractionation on the basis of the inhibitory activity upon the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical cleavage. and repeated column chromatography afforded several antioxidant compounds from Oryza sativa L. The structures and stereochemistry of these compounds were established on the basis of analysis of spectra and some chemical transformations as follows: 5,7-dihydroxy- 8-methoxyflavone, $acacetin-7-O-{\beta}-rutinoside$, $pectolinarigenin-7-O-{\beta}-rutinoside$. At antioxidant activity test for isolated three compounds, antioxidant activity was showed too.

• Natural Product Sciences
• /
• v.12 no.1
• /
• pp.62-66
• /
• 2006

A steroidal saponin, $3-O-{\alpha}-L-rhamnopyranosyl-(1{\rightarrow}2)-[{\alpha}-L-rhamnopyranosyl-(1{\rightarrow}3)]-{\beta}-D-glucopyranosylpennogenin$ ;(1, spiroconazole A) was isolated from the tubers of Dioscorea bulbifera L. var sativa and $^1H-and\;^{13}C-NMR$ assignment was completed using HMBC correlation. In addition, four phenolic substances, 2,7-dihydroxy-4-methoxyphenanthrene (2), quercetin (3), $quercetin-3-O-{\beta}-D-glucopyranoside$ (4), and $quercetin-3-O-{\beta}-D-galactopyranoside$ (5) were also isolated.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.49 no.6
• /
• pp.533-538
• /
• 2004

An introgression line, WH79006 was produced from a single plant from $BC_5F_3$ families from a cross between Hwaseongbyeo used as a recurrent parent and O. minuta (BBCC, Ace. No. 101154) as a donor parent, which was subsequently self-pollinated for three generations. WH79006 resembled the O. sativa parent, Hwaseongbyeo. However it differed from Hwaseongbyeo in several traits including days to heading, culm length, grain size, spikelets per panicle and fertility. These differences in the traits between WH79006 and Hwaseongbyeo can be attributed to the O. minuta introgressions. To detect the introgressions, 294 SSR markers of known chromosomal position have been used. At least, 28 introgressed chromosomal segments have been identified using SSR markers and they map to all chromosomes except chromosome 2. The size of the introgressed segments ranged from 4 to 35cM. A QTL related to culm length was detected using 75 $F_2$ plants from the Hwaseongbyeo/WH79006 cross. This QTL, cl6 located on chromosome 6 explained $9.6\%$ of the total phenotypic variation in the population. This QTL has not been detected in the previous QTL studies between Oryza sativa cultivars, indicating potentially novel alleles from O. minutan.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.50 no.2
• /
• pp.131-141
• /
• 2024

In this study, the efficacy of antioxidant and whitening factors was analyzed in order to verify the possibility of use as functional cosmetic materials related to antioxidant and whitening by using the extract of Oryza sativa cv. Heugjinmi. As a result, S OD-like activity, FRAP, reducing power, xanthine oxidase inhibitory activity, and elastase inhibitory activity were similar to those of the control group. Tyrosinase inhibitory activity had no effect in hydrothermal extract and 59% inhibitory activity in ethanol extract. Ethanol extract was found to inhibit cellular tyrosinase inhibitory activity and melanin biosynthesis at concentrations of 25, 50, and 100 ㎍/mL, which will not affect survival in the B16F10 cell line. In addition, the results of confirming the mRNA expression of tyrosinase, TRP-1, and TRP-2 showed inhibition rates of 37%, 51%, and 34%, respectively, at the highest concentration of 100 ㎍/mL. Therefore, it is believed that O. sativa extract has potential to be utilized as a functional cosmetic material related to antioxidant and whitening.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.10 no.3
• /
• pp.141-146
• /
• 1990

Changes in dry matter yield, crude components, nutrients uptake($P_2O_5$, $K_2O$, CaO, MgO) and sulphur containing amino acid(cysteine, methionine) of orchardgrass(Dacty1is glomerata. L) and alfalfa(A4edicago sativa. L) by gypsum application(as sulphur source, 0, 2. 5, 10, 20kg SIlOa) were investigated to understand the effect of sulphur on herbage production in pasture, which was established in 1987 as means of hand broadcasting. The effect of gypsum on dry matter yield at different cutting times during growing seasons has not been found both in orchardgrass and in alfalfa, but in respect to annual total dry matter yield there were increment in herbage yield (P<0.05) of alfalfa at 5, 10, 20kg SIlOa in 1989 and the amounts of sulphur taken up in herbage slightly increased according to the rates of gypsum application. Maximum apparent recovery of sulphur was 7.55% at 2kg SIlOa in orchardgrass and was 17.8% at 5kg S/lOa in alfalfa. There were no any great differences in the content of crude components of both species and this trend was similar with the mineral contents of orchardgrass. But in alfalfa, the amounts of $K_2O$, CaO, and $P_2O_5$ taken up were increased by gypsum application and the increment in the amounts of minerals taken up in herbage at 20kg SIlOa were 14.9 of $K_2O$, 9.1 of CaO, and 2.5kgIlOa of $P_2O_5$ as compared to those of at untreated plot. Cysteine and methionine were not influenced by gypsum applicaton not only in orchardgrass but also cysteine in alfalfa, however, the content of methionine in alfalfa was slightly increased at 2, 5, lOkg SIlOa and at 20kg SIlOa was reverse.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.94-94
• /
• 2017

Oryza sativa and Oryza glaberrima are rice cultivars distributed in Asia and Africa. There are several differences between these cultivars in morphological characteristics such as panicle structure and especially the secondary branch number of O. glaberrima is less than that of O. sativa. Generally, branch number of panicle related to a large vascular bundle number (VBN) among O. sativa and there is a wide variation of the VBN of the peduncle from where the bundles enter into the rachis branches. However, there is less information about VBN in O. glaberrima and also the relationship between VBN and branch numbers, the primary branch number (PBN) and secondary branch number (SBN). Additionally, the genetic factor for VBN and branch number in O. glaberrima is not completely exploited. In this study, phenotypic variation for VBN and panicle structure were investigated using a set of 40 $BC_3$ -derived from IRGC 104038 (O. glaberrima from Senegal) and 35 $BC_4$ -derived from IRGC103777 (O. glaberrima from Mali) introgression lines with a genetic background of japonica rice Taichung 65. Taichung 65 had 11.8 PBN, 16.0 SBN and 11.5 VBN, while IRGC 103777 had 12.0 PBN, 15.0 SBN and 15.3 VBN. The introgression lines derived from IRGC 104038 had range from 9.0 to 14.4 in the PBN, range from 9.6 to 33.5 in the SBN and range from 9.8 to 14.8 in the VBN. Additionally, the introgression lines derived from IRGC 103777 had range from 9.0 to 18.5 in the PBN, range from 10.3 to 39.0 in the SBN and range from 9.0 to 15.3 in the VBN. Among two set of introgression lines, there are significant correlation between VBN and PBN. Multiple introgression lines indicated higher PBN, SBN and VBN than Taichung 65 and these examined characteristics are supposedly controlled by quantitative traits loci. The genetic factor related to VBN and panicle architecture can be revealed using segregating population in future study.

• Applied Chemistry for Engineering
• /
• v.34 no.2
• /
• pp.137-143
• /
• 2023

In this study, natural surfactants were extracted from Medicago sativa L. The O/W emulsification processes with the extracted natural surfactants were optimized using central composite design model-response surface methodology (CCD-RSM) and a 95% confidence interval was used to confirm the reasonableness of the optimization. Herein, independent parameters were the ratio of saponins to total surfactant (P), amount of surfactant (W), and emulsification speed (R), whereas the reaction parameters were the emulsion stability index (ESI), mean droplet size (MDS), and viscosity (V). Using the multiple reaction, the optimal conditions for the ratio of saponins to total surfactant, amount of surfactant, and emulsification speed for O/W emulsification were 49.5%, 9.1 wt%, and 6559.5 rpm, respectively. Under these optimal conditions, the expected values of ESI, MDS, and V as the reaction parameters were 89.9%, 1058.4 nm, and 1522.5 cP, respectively. The values of ESI, MDS, and V from these expected values were 88.7%, 1026.4 nm, and 1486.5 cP, respectively, and the average experimental error for validating the accuracy was about 2.3 (± 0.4)%. Therefore, it was possible to design an optimization process for evaluating the O/W emulsion process with Medicago sativa L. using CCD-RSM.

• Korean Journal of Breeding Science
• /
• v.40 no.3
• /
• pp.243-249
• /
• 2008

In the previous study, 141 $BC_3F_2$ lines from a cross between the Oryza sativa cv. Milyang 23 and O. glaberrima were used to identify favorable wild QTL alleles for yield component traits. In this study, we carried out QTL analysis of four grain morphology as well as four yield component traits using 141 $BC_3F_5$ lines from the same cross and compared QTLs detected in two different generations. The mean number of O. glaberrima segments in the 141 $BC_3F_5$ lines ranged from 1 to 13 with 2.69 and 5.71 of the average means of homozygous and heterozygous segments, respectively. There was a three-fold difference in the number of QTLs detected for four traits commonly evaluated in two generations (seven QTLs in the $BC_3F_5$ vs 21 in the $BC_3F_2$ population). The percentages of the phenotypic variance explained by QTLs in the BC3F5 population were similar to or less than those in the $BC_3F_2$ population. This is probably due to the difference in the genetic composition of two populations and the environmental effects. The locations of the QTLs commonly detected in both generations were in good agreement except for one QTL for spikelets per panicle. The yield QTL, yd3 was colocalized with the spikelets per panicle, spp3. Yield increase at this locus is due to the increase in spikelets per panicle, because both traits were associated with increase in spikelets per panicle and yield due to the presence of an O. glaberrima allele. Clusters of QTLs for grain morphology traits were observed in two chromosome regions. One cluster harboring five QTLs near SSR markers RM106 and RM263 was detected on chromosome 2. This population would serve as a foundation for development of the introgression line population from a cross between Milyang 23 and O. glaberrima.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2000.11a
• /
• pp.83-85
• /
• 2000