• 한국가금학회지
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• 제34권3호
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• pp.173-180
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• 2007

본 연구는 CLA 전구체 및 정제 부산물의 급여가 산란계의 산란율, 계란 품질 및 면역 관련 혈액학적 지표에 미치는 영향에 대하여 알아보기 위하여 시험을 실시하였다. 사양 시험은 32주령 ISA Brown 252수를 공시하였으며, 5주간 사양 시험을 실시하였다. 실험 설계는 1) CON(basal diet + oat 1% + soybean oil 1%), 2) WOS(basal diet + whey 0.5% + oat 0.5% + soybean oil 1%), 3) WS(basal diet+ whey 1% + soybean oil 1%), 4) WOCLA(basal diet + whey 0.5% + oat 0.5% + CLA produce 1%), 5) WCLA(basal diet + whey 1% + CLA produce 1%) 및 6) FOCLA(basal diet + Fish oil 0.5% + oat 1% + CLA produce 0.5%)로 6개 처리를 하여 처리당 7반복, 반복당 6수씩 완전 임의 배치하였다. 난각 두께는 시험 4주에서 WOS 및 FOCLA 처리구가 WOCLA 및 WCLA 처리구와 비교하여 유의적으로 높게 나타났으며(P<0.05), 난중은 시험 4주에서 WOS 및 FOCLA 처리구가 WS 및 WCLA 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 난황고, 난황색 및 호우 유닛에 있어서 FOCLA 첨가구는 대조구와 비교하여 높게 나타났으며, 종료 시 IgG에 있어서 WOS 및 FOCLA 처리구가 대조구, WS 및 WOCLA 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 결론적으로 어유, 정제 부산물 및 귀리의 첨가는 난각 두께, 난황고, 난황색 및 호우 유닛을 개선시키는 효과를 보여주었으며, CLA 전구체의 첨가 급여는 혈액 내 IgG의 함량을 대조구와 비교하여 증가시켰다.

• 여성건강간호학회지
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• 제6권1호
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• pp.142-152
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• 2000

This study is a descriptive study to offer an actual basic data as Nursing intervention strategy of nurses before delivery in order that pregnant women in Korea may effectively practice Taekyo by examining the relation after apprehending level of recognition and practice of our pregnant women about Taekyo. This study collected questionnaires from 801 pregnant women who visited general hospitals in 10 areas(Seoul, Daejon, chunan, Daegu, Kummi, $Ky{\check{o}}ngju$, $P{\ddot{o}}hang$, Busan, $J{\ddot{o}}nju$, and $Y{\ddot{o}}nkwang$) for prenatal care through an outpatient obstetrics and gynecology from July 15 to August 30, 1999. This study used the tool of lee, Ki Young(1993) revised an complemented by investigator to measure recognition of Taekyo and the tool of Jang, Shun Buk and Park, Young Ju(1996) revised and complemented by investigator to measure practice of Taekyo. The Cronbach's alpha value of each tool was .88 in recognition of Taekyo, while the value was .90 in practice of Taekyo. For data analysis, this study used the descriptive and statistical analysis, Pearson correlation, t-test, ANOVA, Tukey's post hoc contrast, and Stepwise multiple regression in accordance with the purpose of this study with using SPSS Win 7.5 program. The results were as follows ; 1. The practice of Taekyo was low in comparison with recognition of Teakyo by showing average 4.28 points and standard deviation 0.48 at level of recognition of pregnant women about Taekyo on the basis of 5 points and showing to show average 2.81 points and standard deviation 0.36 at practice level on the basis of 4 points. 2. They showed the higher level of recognition on Taekyo at high educational background of pregnant woman(F=3.735, p=.005), Roman catholicism (F=4.570, p=.002), satisfied married life(F=5.448, p=.004), high monthly income(F=6.096, p=.000) and cases of hoping pregnancy(F=2.525, p=.012). 3. They showed the higher level of practice on Taekyo at high educational background of pregnant woman(F=2.883, P=.022), Roman catholicism(F=3.616, p=.032), satisfied married life(F=19.924, p=.000), good health condition(F=2.386, p=.017), cases of hoping pregnancy(F=0.677, p=.000), cases of planning pregnancy with husband(F=3.024, p=.001), cases of regular prenatal care before delivery(F=0.241, p=.005), cases of maternal breast feeding (F=9.132, p=.000), and the number of less children(F=2.763, p=.041). 4. In result of examining correlation between recognition and practice of Taekyo, they showed high level of practice on Taekyo under high level of recognition of pregnant women on Taekyo by showing the statistically significant correlation. 5. In result of examining the related factors that affect recognition and practice of Taekyo y the object, practice of Taekyo had 16.8 percents of explanatory range, purpose of practicing Taekyo 8.5 percents of explanatory range, and monthly income 1.9 percent of explanatory range as variables of affecting recognition of Taekyo. The total explanatory range was 27.2 percents, Recognition of Taekyo had 16.1 percents of explanatory ragne, time of starting Taekyo 3.2 percents, health condition 2.2 percents of explanatory range, condition of hoping pregnancy 1.1 percent of explanatory range, satisfaction of married life 0.8 percent of explanatory range, and religion 0.6 percent of explanatory range as variables of affecting practice of Taekyo. The total explanatory range was 24.0 percents.

• 한국진공학회지
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• 제19권2호
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• pp.81-90
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• 2010

층과 층 사이의 정전기적인력, 수소결합 또는 공유결합을 이용하여 층당 두께를 수 옹스트롱에서부터 수십 나노미터까지 제조할 수 있으며 박막의 표면 형태를 흡착시키고자 하는 물질 및 박막 후처리 공정을 통해 제어할 수 있으며 더 나아가, 삽입하는 물질의 특성에 따라 박막의 기능성을 집적화 및 다양화시킬 수 있다. 본 연구에서는 이러한 층상자기조립방법의 특성을 이용하여 반사방지막, 초소수성 필름 및 전기화학센서로의 응용가능성을 제시하였다. 반사방지막의 경우, 구형의 블록공중합체를 유리기판 위에 다층박막으로 적층시킴으로써 박막 굴절률을 1.25까지 감소시켰고 이를 통해 약 99.5%의 빛 투과도를 달성할 수 있었다. 더 나아가 바이오물질인 엔자임을 다층박막에 삽입시킬 경우, 활성 산소를 분해시키는 전기화학센서로의 제조가 가능함을 보인다. 본 연구는 본인이 이미 발표한 논문(J. Am. Chem. Soc. 128, 9935 (2006); Adv. Mater. 19, 4364 (2007); Electro. Mater. Lett. 3, 163 (2007))들을 정리하여 층상자기조립법에 관해 소개하는 논문이다.

• 미생물학회지
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• 제30권4호
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• pp.305-309
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• 1992

Bacillus stearothermophilus 의 cytosine deaminase (EC 3. 5. 4.1 )를 수율 52.7% 로 7.2배 부분정제했다. 부분정제된 cytosine deaminase 는 cytosine 을 유일한 기질로 이용하였으머 5-methylcytosine 과 5-fluorocytosine 은 기질고 이용되지 못햇으며 cytosine 에 대한 Michaelis 정수 Km 값은 5.9 mM 이었다. 본효소는 pH 4.0 에서 7.0 까지의 폭 넓은 pH 영역에서 안정했으며 80.deg.C 에서 10 분간 열처리하여도 75% 이상의 효소활성이 잔존하여 높은 내열성 효소였다. 본 효소의 반응최적 pH 는 7.0-7.5 였으며 반응 최적 온도는 35. 37.deg.C 였다. 그리고 Arrhenium plot 에 의하여 계산된 활성화 에너지 값(Ea value) 은 26 Kcal/mol 이었다. 본 효소는 중금속인 1 mM의 $Cd^{2+}$ , $Hg^{2+}$ 및 $Cu^{2+}$ 에 의하여 완정히 실활되었으며 GMP 와 CMP 에 의해서는 효소활성이 촉진되었다. 특히 본 효소는 p-chloromercuribenzoate 에 의하여 효소 활성이 강하게 저해되어 thiol 효소임을 추정할 수 있었다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권17호
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• pp.7803-7807
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• 2015

Opisthorchis viverrini infection is a serious public health problem in Southeast Asia especially in the northeast and north of Thailand. Therefore, a cross-sectional survey using multistage sampling was conducted from the rural communities of Surin province, Thailand, during September 2013 to July 2014. O. viverrini infection was determined using Kato's thick smear technique. Socio-demographic, information resources, and history data were collected using predesigned semi-structured questionnaires. A total of 510 participants completed interviews and had stools collected. Some 32 (6.47%) participants were infected with O. viverrini. The rate was slightly higehr in males (6.61%) than females (6.32%). High frequencies were found in the age groups 61-70 (19.4%) and 71-80 years (19.4%), those involved in agriculture (10.5%), and in primary school (10.3%). The distribution of high infection was found in Tha Tum (16.7%) and Sankha district (16.7%), followed by Samrong Thap (13.3%), Si Narong (13.33%), and Buachet district (13.33%). Chi-square testing indicated that age (61-70 and 71-80 year old), education (primary school) and occupation (agriculture), were significantly associated with O. viverrini infection (p-value<0.05). Of 72.6% participants who had past histories with stool examination, 17.0% of them had been infected with O. viverrini and 43.2% treated with praziquantel. This finding confirmed that O. viverrini is still a problem in Surin province, Thailand, and therefore, interventions are urgently required for mass treatment and health education implementation.

• Nuclear Engineering and Technology
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• 제55권7호
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• pp.2662-2669
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• 2023

For the precise measurements of special nuclear materials (SNM) including Pu and Am isotopes, we have used phoswich detector combination of two single crystal scintillators of Gd₃Ga₃Al₂O₁₂:Ce and CsI:Tl. High detection efficiency and sensitivity along with high figure of merit for the discrimination of these phoswich detectors ensures the detection and discrimination of thermal neutrons and gammas from spontaneous fission of Pu and other isotopes in presence of high gamma background. Using this detector, the low energy gammas, which is stopped completely in 1mm thick disc of GGAG, can be also discriminated from high energies gamma and shows linearity in wide range of sample quantities. By changing only the appropriate shielding, the similar setup was used for thermal neutron detection and shows a very good linearity over wide range. The quantity of a test sample was also calculated accurately by using the measured calibrated plot.

• 한국수정란이식학회지
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• 제17권1호
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• pp.13-21
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• 2002

Intracytoplasmic Sperm Injection (ICSI) has been widely used fur both human infertility and basic research. However, the high incidence of chromosomal abnormality is severe problem in cattle. Various oocyte activation stimuli, therefore, were compared by assessment of developmental capacity and chromosome analysis. Motile sperm selected by Percoll-density gradient were treated with 5 mM dithiothreitol (DTT) and injected into an oocyte matured fur 24 h. Eggs were then allocated into 5 treatment groups. Group 1 (control), sperm injection was performed without any further activation stimuli to the oocytes. Group 2 (handled control), sham injection was performed without sperm. In Group 3, oocytes exposed to 5 (M ionomycin for 5 min at 39(C. Group 4. ionomycine + 1.9 mM demethylaminopurine (DMAP, 3 h) and Group 5, ionomycine + 3 h culture in Ml99 + DMAP. Cleavage and the later development rate in Groups 1, 2 and 3 were significantly (P<0.05) lower than those in Groups 4 and 5. The incidence of chromosomal abnormality in the embryos treated directly with DMAP after ionomycine was relatively higher than in the embryo of Group 3 h, delayed DMAP treatment. From this results DMAP caused to be arrested the release of the 2nd polar body, resulting in changes of chromosomal pattern. Therefore, the time interval between ionomycin and DMAP is a crucial role in bovine ICSI.

• 한국식품영양과학회지
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• 제34권3호
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• pp.361-365
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• 2005

본 연구는 식용유황으로 사용되고 있는 MSM(methyl sulfonyl methane)을 돼지 식이에 첨가하였을 때 성장 능력과 영양소 소화율에 미치는 영향을 평가하기 위하여 실시하였다. 시 험 1에서 는 3원 교잡종(Landrace × Yorkshire × Duroc) 육성돈 60두를 공시하였으며 시험개시시의 체중은 48.15±0.15㎏이었고 35일간 실시하였다. 시험설계는 옥수수-대두박 위주의 식이로서 처리한 대조구(Cotrol: 대조구), 대조구 식이 내 MSM 0.01%(T1: 대조구＋0.01% MSM), 0.02%(T2: 대조구＋0.02% MSM)로 3처리를 하여 처리당 5반복, 반복당 5마리씩 완전임의 배치하였다. 일당증체량과 일당식이섭취량은 T1 처리구가 높은 경향을 보였으나 유의적인 차이는 없었다(p＆gt;0.05). 식이효율과 영양소 소화율 역시 처리구간에 유의적인 차이가 없었다(p＆gt;0.05). 시험 2에서는 3원 교잡종 육성돈 100두를 공시하였으며, 시험 개시시 체중은 38.85±0.15㎏이었다. 시험설계는 옥수수-대두박 위주의 기초식이 대조구(CON), 대조구 식이에 황산네오마이신 0.05%, OTC(oxytetracycline) 0.05% 첨가구(T1), 대조구 식이에 MSM 0.01% 첨가구(T2) 및 대조구 식이에 황산네오마이신 0.05%, OTC 0.05%와 MSM 0.01% 첨가구(T3)로 4개 처리로 하였다. 일당증체량은 MSM 0.01% 첨가한 T2구와 항생제와 MSM 0.01% 첨가한 T3구가 유의적으로 높았다(p＆lt;0.05). 일당식이섭취 량에서 는 T2 처리구와 T3 처리구에서 증가함을 보였다(p＆lt;0.05). 식이효율은 대조구에서 가장 높은 경향을 보였다(p＆lt;0.05). 결론적으로, 실험 1과 2에서 알 수 있듯이 돼지에 있어 사료내 MSM의 첨가는 성장율을 향상시킬 것으로 기대되고 앞으로 많은 연구가 필요할 것으로 사료되며 항생제와의 혼합 첨가는 성장율과 영양소 소화율을 향상시키는 효과가 있을 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권9호
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• pp.1271-1276
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• 2004

For Exp. 1, one hundred twenty Duroc${\times}$ Yorkshire${\times}$Landrace pigs (6.34${\pm}$ 0.70 kg average initial BW) were used in a 35 d growth assay. There were six pigs per pen and four pens per treatment. Dietary treatments included 1) NC (negative control; basal diet), 2) PC (positive control; added 5% HP $300^{(R)}$, HAMLET PROTEIN A/S, as protein source), 3) PSP 2.5 (added 2.5% $Pepsoygen^{(R)}$, Genebiotech Co. Ltd., as protein source), 4) PSP 5.0 (added 5% $Pepsoygen^{(R)}$ as protein source), and 5) PSP 7.5 (added 7.5% $Pepsoygen^{(R)}$ as protein source). For d 21, ADFI was increased in pigs fed PSP diets compared with PC diet (p<0.05). ADG was tended to improve as concentration of PSP in the diets was increased. For d 21-35 and entire experimental period, pigs fed PSP diets had more feed intake than pigs fed NC or PC diets (p<0.05). For the whole period, ADG was improved in PSP treatments compared with NC treatment (Linear effect, p=0.05). On d 21 and d 35, digestibilities of DM and N were higher (p<0.05) for pigs fed PSP diets than pigs fed NC diet and PSP 2.5 treatment was the highest among the treatments. On d 21, digestibility of N was significantly improved in PSP treatments compared with PC treatment (p<0.05). For Exp. 2, five Duroc${\times}$Yorkshire${\times}$Landrace barrows (8.74${\pm}$0.22 kg average initial BW) were surgically fitted with a simple T-cannulas approximately 15 cm prior to the ileo-cecal junction. The experimental designs were 5${\times}$5 latin squares with pigs and periods as blocking criteria. Dietary treatments were same as Exp.1. Generally, apparent ileal digestibilities of DM and N were higher for pigs fed PSP diets than pigs fed NC or PC diets (p<0.05) and PSP 5.0 and 2.5 treatments were the highest among the treatments, respectively. Apparent ileal digestibilities of the whole amino acids were increased in PSP treatments compared with NC treatment (Quadratic effect, p${\leq}$0.05). Also, histidine, lysine and threonine digestibilities in essential amino acids and alanine, asparatic acid and glycine digestibilities in non essential amino acids were significantly higher for pigs fed PSP diets than pigs fed PC diets (p${\leq}$0.05). In conclusion, feeding processed soy protein to weanling pigs increased growth performance, nutrient digestibility and apparent ileal digestibilities of DM, N and most amino acids.

• 미생물학회지
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• 제9권3호
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• pp.103-120
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• 1971

We have been studied on brewing sweet starch. We obtained the results as follows ; 1) 5 strains, T-T-2, T-T-4, T-K-2, T-T-18, T-T-1, were the most available in view of fermentative power by capacity of $CO_2$. 2) 5 strains, T-T-4, T-T-2, T-T-1, T-T-3, T-K-2, produced capacity of alcohol more than 5.78%. 3) 6 strains, T-T-2, T-K-2, T-T-4, T-S-2, T-I-3, T-I-1, are available not only taste and flavour, but productive power of alcohol in sweet potato starch. 4) The form of 6 strains are long oval and round and most of them are similar to the other yeast in size. 5) In giant colony the color was cream color and cream buff, and T-K-2 was formed by $15{\times}12mm$ on diameter and by 3.5mm on high. 6) Optimum temperature of most of all strains is 25~ $300^{\circ}C$but T-K-4 is 28-30.deg.C. 7) Optimum pH is 3.4-4.6. 8) T-S-2 was died off at 65.deg.C, the other strains died $60^{\circ}C$. 9( Making Bun-kok with non-heated wheat bran .alpha.-amylase was more increased by 4.5-13.5 mg of glucose in reaction solution and .betha.-amylase more 1.6-3.4ml of N/10-$KMnO_4$ Solution than Bun-kok with heated wheat bran. 10) It seems that mycellium grows better than original in substance containing 0.4 ~ 1.2% of HCl. 11) Making Bun-kok to add 0.8% HCl, .alpha.-amylase was increased 9.93-20.7mg of glucose and .betha.-amylase ws increased 2.6~4.3ml of N/10-$KMnO_4$ solution to reaction solution. 12) 1.2%-HCl, or higher concentration, acts as inhibitor, in the meanwhile the concentration between 0.4~0.8% of HCl acts as activator. 13) We must make Bun-kok for 42 hours, at 28~$30^{\circ}C$) After we made Bun-kok using S-O-II and R-J-I one by one, Bun-kok which mix each other in equal quantity is increased more than original on enzyme acrivity. 15) Oxidation is the best way of refining sweet potato starch in N/10-phosphate buffer solution (pH 7.5). 16) When we prepared sweet potato starch, first pH was 3.0.