• Applied Biological Chemistry
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• v.24 no.4
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• pp.224-229
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• 1981

Perilla (Perilla frutescens Britton var. Crispa Decasne) cultivated in three geographical areas of Korea, Gwangju, Taegu and Jeju, was analyzed for carbohydrate, lipid, protein, inorganic components and fatty acid composition. Carbohydrate, lipid and protein content of perilla seed ranged from 40 (Taegu) to 44% (Jelu), from 28 (Jeju) to 34% (Taegu) and from 15 (Gwangju) to 16% (Jeju), respectively. The overage contents of potassium, silicate, calcium, phosphate, iron, zinc, magnesium and mangane in the perilla seed varied between $426{\sim}446$, $197{\sim}229$. $124{\sim}136$, $46{\sim}56$, $30{\sim}49$, $42{\sim}45$, $40{\sim}45$, and $30{\sim}36mg%$, respectively and those variations different cultivation areas were not significant except iron. Saponification number, iodine value and acid value of the perilla oil were between $194{\sim}198$, $196{\sim}200$ and $4{\sim}5$, respectively. Those variations among the cultivation areas were not significant. The composition of the perilla oil was observed to be composed of $92{\sim}95%$ of triglyceride, $1.2{\sim}1.3%$ of phospholipid, $1.7{\sim}1.9%$ of unsaponifiables, $0.7{\sim}0.9%$ of free fatty acid, and $1.1{\sim}1.4$ linolenic, linoleic and oleic acid as $55{\sim}56$, $16{\sim}18$ and $16{\sim}20%$ of total fatty acids, but in phospholipid, the content of saturated fatty acids, $12{\sim}24$ of total fatty acid, was higher than that in triglyceride, $8{\sim}19%$ of total fatty acids. The content of saturated fatty acids in sterylester $(14{\sim}19%)$ was higher than that in sterylglycoside ($6{\sim}7%$ of total fatty acids). The variation in fatty acid composition was not significant in the composition of total fatty acid but a significant difference was observed in the composition of phospholipid, in which the content of palmitate ranged from 11.8%(Taegu) to 24%(Gwangju) of total fatty acids. No significant variation was found in the fatty acid composition among the cultivation areas, while a significant difference was observed in phospholipid.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.5
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• pp.399-407
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• 2009

Laser induced breakdown spectroscopy(LIBS) is an simple analysis method for directly quantifying many kinds of soil micro-elements on site using a small size of laser without pre-treatment at any property of materials(solid, liquid and gas). The purpose of this study were to find an optimum condition of the LIBS measurement including wavelengths for quantifying soil elements, to relate spectral properties to the concentration of soil elements using LIBS as a simultaneous un-breakdown quantitative analysis technology, which can be applied for the safety assessment of agricultural products and precision agriculture, and to compare the results with a standardized chemical analysis method. Soil samples classified as fine-silty, mixed, thermic Typic Hapludalf(Memphis series) from grassland and uplands in Tennessee, USA were collected, crushed, and prepared for further analysis or LIBS measurement. The samples were measured using LIBS ranged from 200 to 600 nm(0.03 nm interval) with a Nd:YAG laser at 532 nm, with a beam energy of 25 mJ per pulse, a pulse width of 5 ns, and a repetition rate of 10 Hz. The optimum wavelength(${\lambda}nm$) of LIBS for estimating soil and plant elements were 308.2 nm for Al, 428.3 nm for Ca, 247.8 nm for T-C, 438.3 nm for Fe, 766.5 nm for K, 85.2 nm for Mg, 330.2 nm for Na, 213.6 nm for P, 180.7 nm for S, 288.2 nm for Si, and 351.9 nm for Ti, respectively. Coefficients of determination($r^2$) of calibration curve using standard reference soil samples for each element from LIBS measurement were ranged from 0.863 to 0.977. In comparison with ICP-AES(Inductively coupled plasma atomic emission spectroscopy) measurement, measurement error in terms of relative standard error were calculated. Silicon dioxide(SiO2) concentration estimated from two methods showed good agreement with -3.5% of relative standard error. The relative standard errors for the other elements were high. It implies that the prediction accuracy is low which might be caused by matrix effect such as particle size and constituent of soils. It is necessary to enhance the measurement and prediction accuracy of LIBS by improving pretreatment process, standard reference soil samples, and measurement method for a reliable quantification method.

• Childhood Kidney Diseases
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• v.2 no.1
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• pp.26-33
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• 1998

This retrospective study was designed to evaluate the prognostic value and histologic correlation of sonography in childhood nephrotic syndrome. Sixty-nine patients with proteinuria over 2g per day at the time of presentation who were treated at the Korea University Hospital were included in this review. They were 1 to 15 years old(mean age, 7.8 years) with 49 males and 20 females. In each patient an ultrasound examination was done using SPA 1000(Diasonics, C.A., U.S.A.) on admission. Tissue specimens were obtained from 46 patients. The paraffin-embedded specimens were reviewed with special reference to interstitial edema, interstitial fibrosis, tubular atrophy, global sclerosis or inflammatory cell infiltrates. Biopsy proven renal disease were minimal change disease(n=20), focal segmental glomerulosclerosis(n=7), membranous glomerulonephritis(n=2), membranoproliferative glomerulonephritis(n=1), $Henoch-Sch\"{o}nlein$ purpura nephritis(n=6), IgA nephropathy(n=5), poststreptococcal glomerulonephritis(n=2), systemic lupus erythematosus(n=1) and Alport syndrome(n=2). There was a significant relationship between increased cortical echogenicity and global sclerosis or tubular atrophy(P<0.05). But no significant relationship was found between increased cortical echogenicity and interstitial fibrosis, interstitial edema, or inflammatory cell infiltration. In biopsy-proven primary nephrotic syndrome(n=30), no significant relationship was found between the increased conical echogenicity and the interstitial edema, interstitial fibrosis, global sclerosis, tubular atrophy or inflammatory cell infiltration. But there was a significant relationship between increased cortical echogenicity and resistance to corticosteroid (P<0.05). These results suggest that increased cortical echogenicity may be due to tubular atrophy or global sclerosis in patients with proteinuria and may be an effective indicator of resistance to corticosteroid in primary nephrotic syndrome.(J Korean Soc of Pediatr Nephrol 2:26-33, 1998)

• Korean Journal of Weed Science
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• v.2 no.1
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• pp.57-62
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• 1982

This study was conducted to evaluate the important weed occurring in orchard and to establish on effective control method using combination of non-selective foliar applied herbicide followed by soil treated herbicide. In terms of quantity, Calysregia hederacea, Artemisia princeps, Ixeris chinensis, Cephalonopios segetum, Capsella bursa-pastoris, Setaria viridis, Chenopodium album, Trifolium repens, Digitarta sanguinalis in order were observed to be the most important weeds in May, In July the most dominant weeds were D. sanguinalis, Setaria viridis, Amaranthus lividus, Portulaca oleracea, Polygonum hydropiper, Acalypha australis, Artemlsia prtnceps, Miscanthus sinensis in order, showing D. sanguinalis to be the dominant species. D. sanguinalis was one single dominant species till autumn, followed by A. lividus, P. oleracea, A. australis. Simpson's index, 0.159 obtained in May just before herbicide application increased to 0.253 in July and again decreased to 0.233 in Sept. in the oxyfluorfen treated plot, meaning that no specific weed species were dominant, while in the untreated plot, D. saguinalis was relatively single dominant species, showing Simpson's index, 0.540 and this trend was mainained till autumn. The most effective herbicide to control D. sanguinalis and other annuals was oxyfluorfen at 0.47kg a.i./ha. when applied at preemergence treatment, showing more than 80% control of them for 65 days. The results suggest that paraquat at 32/ha (prod.) as the foliar application followed by oxyfluorfen 0.47kg a.i./ha applied as the preemergence at one week after paraquat treatment can maintain the clean orchard more than 65 days, showing one of the promising and applicable control system in orchard.

• Korean Journal of Weed Science
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• v.2 no.2
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• pp.160-168
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• 1982

Nine combined dosal levels of Paraquat and Oxyfluorfen were compared on pear orchard to control Digitaria adscendens Henr, and others, namely Commelina communis L., Erigeron annuus L., Echinochloa crusgalli P. Beauv var. oryzicola Ohwi, etc.. By treating as an early-postemergence, the mixtures showed higher control effects than 85% of the whole weeds, but the control effects in the mono-treatments of respective herbicide were only 20% or so. On the other hand, as a late-postemergence, the control rates were fluctuated from 31% to 94%. However, there was no significant difference in Spearman's rank correlation coefficients between both efficacies. Thus indicate that the action style of synergistic effect was not influenced by the application time, and the levels of mixtured dosages should be adjusted by the application times and interfering conditions.

• Korean Journal of Food Science and Technology
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• v.14 no.4
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• pp.291-300
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• 1982

In order to develop effective and simple sanitation method for the extention of shelf-life of fresh poultry meat, the effect of sanitizers, sanitation methods and packaging materials on the extention of shelf-life of poultry meats was observed at the $4^{\circ}C$ and room temp$(10{\sim}20^{\circ}C)$. The results are summarized as follows: 1. The autochonous skin microflora of poultry, before processing, were believed to be removed or killed during the scalding and plucking, and exposed dermal tissue was contaminated by microorganisms from the subsequent stages of processing. 2. In the final stage of poultry processing, total viable counts of microorganisms and coliforms were averaged to $3.5{\times}10^4/cm^2$ and $400/cm^2$, respectively. 3. The refrigerated shelf-life of fresh whole poultry carcasses at $3\;to\;4^{\circ}C$ was extended to 7 to 16 days compared to control with the various treatments of some sanitizers by dipping freshly chilled carcasses for 5 min or spraying 1 liter of sanitizers per carcasses. In the case of storage at $10\;to\;15^{\circ}C$, the shelf-life of poultry carcasses was extended to one to two days by the sanitation treatments compared to control. 4. Spraying sanitation was more effective than dipping sanitation, and 5 minutes dipping and one liter spraying per carcass were enough for effective sanitation of poultry carcasses in most sanitizers. 5. The packaging with an oxygen impermeable polyvinylidene chloride extended the shelf-life to 10 days and 5 days with polyethylene compared to control. When poultry carcasses were sanitized by continuous spraying with one liter of 30 ppm of chlorine and another one liter of 5% of potassium sorbate, packaged with polyvinylidene chlorlde were extended to about 30 days compared to control.

• Journal of Environmental Impact Assessment
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• v.24 no.4
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• pp.352-366
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• 2015

The purpose of this study is to offer the basic data to the habitat conservation and management by surveying and analysing the ecological characteristics such as the flora, vegetation structure and soil of Rhodotypos scandens habitat. The flora were summarized as 131 taxa including 57 families, 99 genera, 107 species, 1 subspecies, 17 varieties and 6 forms. The life forms grouped as in the follows; the megaphanerophytes(MM) were 21 taxa, 24 taxa of the microphanerophytes(M), 30 taxa of nanophanerophytes(N), 6 taxa of chamaiphytes(Ch), 20 taxa of hemicryptophytes(H), 17 taxa of geophytes(G), 12 taxa of therophytes(Th) and 1 taxa of hydrophytes(HH). The present conditions of communities were 8 types including Zelkova serrata, Quercus aliena, Ulmus parvifolia, Rhamnella frangulioides, Castanea crenata, Albizia julibrissin, Celtis sinensis and Robinia pseudoacacia. In case of the dominant species by layers, the tree layer species were Zelkova serrata, Quercus aliena, Ulmus parvifolia, Castanea crenata, Albizia julibrissin, Celtis sinensis and Robinia pseudoacacia, and the subtree layer species were Rhamnella frangulioides, Q. aliena, Z. serrata, A. julibrissin, U. parvifolia and Broussonetia kazinoki. The shrub layer species was Rhodotypos scandens. In the results of analyzing the diversity index, $H^{\prime}{_{max}}$ from 1.691 to 2.610, from 2.197 to 3.466 in H'max, from 0.646 to 0.903 in J' and from 0.097 to 0.354 in D. In the results of analysing the soil, there showed that acidity was 5.6, 0.5dS/m of electrical conductivity(EC), 59.1mg/kg of available $P_2O_5$, 49.7% of organic matter content, $0.4cmol^+/kg$ of exchangeable $K^+$, $13.5cmol^+/kg$ of exchangeable $Ca^{2+}$, $3.3cmol^+/kg$ of exchangeable $Mg^{2+}$.

• Korean Journal of Weed Science
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• v.6 no.2
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• pp.110-123
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• 1986

To know the ecological pattern of bulrush (Scirpus juncoides Roxb.) seeds and overwintreed stumps in germination and sprouting responses as affected by different temperature (7 trt.), light intensity (5 trt.), shading intensity (S trt.), light quality (specturm spectrum; 6 trt.), soil acidity (7 trt.), stump sizea (weight base; 5 trt.), and molding depth (6trt.), respectively, this serial studies were conducted by use of growth chamber, incubator, Wagner pot and petri-dish. Most efficient treatment was obtained from 25-$35^{\circ}C$ temperature, higher light intensity in 2-11 klux range, 95% shading intensity, clear and yellow film for seeds/clear and blue film for stumps, soil pH 5.53, 3-4g stump weight, 0-5% wxygen concentration, 1 ㎝ flooding depth for seeds, and 1-1.5cm molding depth for seeds/0.5-1.0 cm molding depth for stumps, respectively, among others.

• Journal of Technologic Dentistry
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• v.19 no.1
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• pp.21-35
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• 1997

The Ag-Pd-Cu alloys containing a small amount of Au is commonly used for dental purposes, because this alloy is cheaper than Au-base alloys for clinical use. However, the most important characteristic of this alloy is age-hardenability, which is not exhibited by other Ag-base dental alloys. The specimens used were Ag-20Pd-20Cu ternary alloy and Au addition alloy. These alloys were melted and casted by induction electic furace and centrifugal casting machine in Ar atmoshpere. These specimens were solution treated for 2hr at $800^{\circ}C$ and were then quenched into iced water, and aged at $350{\sim}550^{\circ}C$ Age-hardening characteristics of the small Au-containing Ag-pPd-Cu dental alloys were investigated by means of hardness testing, X-ray diffraction and electron microscope observations, electrical resistance, differential scanning calorimetric, emergy dispersed spectra and electron probe microanalysis. Principal results are as follows : Hardening occured in two stages, I. e., stage I in low temperature and stage II in high temperature regions, during continuous aging. The case of hardening in stage I was due to the formation of the Llo type face centered tetragonal PdCu-ordered phase in the grain interior and hardening in stage I was affedted by the Cu concentration. In stage II, decomposition of the $\alpha$ solid solution to a PdCu ordered phase(L1o type) and an Agrich ${\alpha}2$ phase occurred and a discontiunous precipitation occurred at the grain boundary. Form the electron microscope study, it was concluded that the cause of age-hardening in this alloy is the precipitation of the PdCu ordered phase, which has AuCu I type face-centered tetragonal structure. Precipitation procedure was ${\alpha}\to{\alpha}+{\alpha}2+PdCu\to{\alpha}1+{\alpha}2+PdCu$ at Pd/Cu = 1 Ag-Pd-Cu alloy is more effective dental alloy as ageing treatment and is suitable to isothermal ageing at $450^{\circ}C$.

• Applied Microscopy
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• v.15 no.2
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• pp.31-68
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• 1985

Authors are trying to unveil the ultrastructural organization of olfactory bulb, which has been summerized under light microscopic level or communicated only in some detail in different view point until now. For the critical point of view, since the phylogenetical approach will give the ultimate value in the correlative study between structural and functional bases (Brodal, 1969), the present study was carried out light and electron microscopic analyses of the structures of the neurons and synaptic organizations in olfactory bulbs from different animals in phylogenetical scale. We selected each one species from five animal classes: the house rabbit(Oryctolagus cuniculus var. domesticus [Gmelin]) from Mammalia, the domestic fowl (Gallus gallus domesticus Brisson) from Aves, the viper (Agkistrodon hylys [G.P. Pallas]) from Reptilia, a frog (Bombiana orientalis Boulenger) from Amphibia and the crussian carp (Carassius carassius [Linne]) from Pisces. For light microscopic study, samples were fixed in 10% formalin and paraffin sections were stained with hematoxylin-eosin. For the electron microscopic study, the tissues were fixed by perfusion through the heart or immersion with 1% paraform-aldehyde-glutaraldehyde mixture (phosphate buffer, pH 7.4), and final tissue block trimmed under dissecting microscope were osmicated (1% OsO4), they were embedded in Araldite or Epon 812, and ultrathin sections were made by LKB-V ultratome following the inspection of semi-thin sections stained with toluidine blue-borax solution. Ultra-thin sections contrasted with uranyl acetate and lead citrate were observed with JEM 100CX electron microscope. We have summerized our morphological analyses as follows: 1. The olfactory bulb of rabbit, viper and frog shows the eight layers of fila olfactoria, glomerular, external granular, external plexiform, mitral cell, internal plexiform, internal granular, medullary but domestic fowl shows the five layers of glomerular, fibrillar, mitral, granular and medullary and the three layers of fibrilla, glomerular and medullary in crussian carp. The sharpness of demarcation between the layers shows deferential tendency according to phylogenetical order. 2. Mitral cells of vertebrate have large triangular or oval shape with spherical nuclei which contain not so much chromatin. The cytoplasm contains numerous cell organelles, of which Nissl's bodies or granular endoplasmic reticula arranged as parallel strands. Development of granular endoplasmic reticula were declined as the phylogentical grade is going lower. 3. Tufted cells of all animal are mostly spindle or polygonal contour and contain oval nuclei which located in periphery of cytoplasm. The nuclei of rabbit, fowl, viper and frog has relatively space chromatin, but a nucleus of crussian carp contain irregularly aggregated chromatin in karyoplasm. Their cytoplasmic volume and cell organelle contents are in between those of mitral cell and granular cell. They contain moderate amount of mitochondria, granular endoplasmic reticula, a few Golgi complex, polysomes, lysosome, etc. 4. Granule of cells of all the vertebrate amimals studied exhibit similar features; cells and their dense nuclei show spherical or oval contour, and they have the thin rim of cytoplasm which contain only a few cell organelles. 5. In rabbit, the soma of mitral cells were in contact with boutons with two types of synaptic vesicles, that is, round and flat vesicles, especially flat vesicles in boutons were showing reciprocal synapses. However, in domestic fowls, vipers, frogs and crussian carps, there were found boutons showing only spherical synaptic vesicles. 6. The boutons containing round synaptic vesicles were made contact with the some of tufted cell of olfactory bulb in the rabbits, fowls, vipers and frogs, but no synaptic boutons were observed in soma of tufted cells in crussian carps. In the frogs, there were observed dendrites were contact with the soma of tufted cells. 7. In the neuropils of plexiform, granular and glomerular layers olfactory bulbs in the vertebrate, the synapses were axo-large dendrites, axo-median and small dendrites, dendrodendritic, and axo-axonal contacts. However, in the neuropil of crussian carps, synapses were observed only in glomerular layer.