• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.4
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• pp.634-639
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• 1997

In order to obtain the basic knowledges for the effective seed production of Perinereis aibuhitensis, sediment preference, burrow shape and behavior with in burrow were investigated in the laboratory. The highest value in both sediment preference and survival rate of P. aibuhitensis were shown at fine sand below 0.10 mm in the mean diameter. The worm made various types of burrows, such as J, L, O, Y and I shapes. Generally, only one individual inhabits in a burrow with head-up, but when excreting, it positions up-side down.

• Korean Journal of Head & Neck Oncology
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• v.6 no.1
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• pp.34-39
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• 1990

This is a retrospective analysis of 8 patients with olfactory neuroepithelioma treated by external radiation during 1981-1988. Their age ranged from 13 to 65 years. All of them were male patients. At the time of diagnosis, six patients were classified as Kadish stage C, two were stage B, and none of them were stage A. Six of eight patients treated by external irradiation, only one patient had complete surgical resection followed by postoperative irradiation. Another one patient received one course of induction chemotherapy followed by radical irradiation. Three patients developed local recurrences and five patients had distant metastasis. One patient with stage B disease who had complete surgical resection followed by postoperative irradiation was alived without evidence of disease. Seven of eight patients died of tumor, with a duration of survival ranging from 3 to 106 months.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.7
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• pp.4151-4155
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• 2013

Background: To compare response evaluation criteria in solid tumours (RECIST) and volumetric evaluation (VE) for colorectal cancer with liver-limited metastasis. Patients and Methods: VE of liver metastases was performed by manual contouring before and after chemotherapy on 45 pairs of computed tomography (CT) images in 36 patients who suffered from metastatic colorectal cancer (mCRC) with liver metastasis only. Cohen kappa was used to compare the agreement between VE and RECIST. Pearson correlation was performed for their comparison after cubic root transformation of the aggregate tumor volumes. Logistic regression was done to identify clinical and radiographic factors to account for the difference which may be predictive in overall response (OR). Results: There were 16 partial response (PR), 23 stable disease (SD) and 6 progressive disease (PD) cases with VE, and 14 PR, 23 SD and 8 PD with RECIST. VE demonstrated good agreement with RECIST (${\chi}$=0.779). Discordant objective responses were noted in 6 pairs of comparisons (13.3%). Pearson correlation also showed excellent correlation between VE and RECIST ($r^2$=0.966, p<0.001). Subgroup analysis showed that VE was in slightly better agreement with RECIST for enlarging lesions than for shrinking lesions ($r^2$=0.935 and $r^2$=0.780 respectively). No factor was found predictive of the difference in OR between VE and RECIST. Conclusions: VE exhibited good agreement with RECIST. It might be more useful than RECIST in evaluation shrinking lesions in cases of numerous and conglomerate liver metastases.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.1
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• pp.33-38
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• 2004

This study was aimed at testing the gene expression of antioxidant enzymes and apoptosis genes for in vitro culture in porcine embryos produced by in vitro maturation/in vitro fertilization (IVM/IVF). Pocine preimplantation embryos obtainted from IVM/IVF can be successfully culture in vitro, but they are delayed or stop to develop at specific developmental stage. Many factors such as reactive oxygen species and apoptosis in an IVM/IVF system followed by in vitro culture influence the rate of production of viable blastocysts. Porcine embryos derived from IVM/IVF were cultured in the atmosphere of 5% $CO_2$ and 20% $O_2$ at $38.5^{\circ}C$ in NCSU23 medium. The patterns of gene expression for antioxidant enzymes and apoptosis genes during in vitro culture in pocine IVM/IVF embryos were examined by the modified semi-quantitative single cell reverse transcriptase-polymerase chain reaction (RT-PCR). Porcine embryos produced by in vitro procedures were expressed mRNAs for CuZn-SOD, GAPDH and GPX, whereas transcripts for Mn-SOD and catalase were not detected at any developmental stages. Expression of caspase-3 mRNA was detected at 2 cell, 8 cell 16 cell and blastocyst, but p53 mRNA was not detected at any stages. The fas transcripts was only detected in blastocyst stage. These results suggest that various antioxidant enzymes and apoptosis genes play crucial roles in vitro culture of porcine IVM/IVF embryos.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.1
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• pp.103-106
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• 2008

A broiler experiment was conducted to compare the effects of supplementary iron sources and levels on the iron content of broiler meat. Two hundred and fifty hatched Ross broiler chickens were randomly assigned to 5 dietary treatments. Each treatment had 5 replicates of 10 birds (5 males and 5 females). Birds were housed in raised floor batteries and fed traditional broiler diets ad libitum for 5 weeks. Dietary treatments were as follows: Control, Fe-Met 100 (100 ppm iron as Fe-methionine), Fe-Met 200, $FeSO_4$ 100 (100 ppm iron as $FeSO_4{\cdot}7H_2O$) and $FeSO_4\;200$. There were no significant differences among treatments in parameters related to production performance. Liver contained approximately 10 times more iron than the leg muscle which contained approximately 3 times more iron than either breast muscle or wing muscle. Significant differences in iron content in the broiler meat were observed. In the breast meat, Fe-Met treatments were significantly (p<0.05) higher than other treatments in iron content. In the leg meat, Fe-Met treatments and $FeSO_4\;200$ treatment were significantly higher than the control in iron content. In the wing muscle, Fe-Met 200 treatment was significantly higher than other treatments in iron content. Iron content in the liver was significantly influenced by source and supplementation level of iron. Fe-Met treatments were higher than $FeSO_4$ treatments and 200 ppm treatments were higher than 100 ppm treatments in iron content in the liver. It is concluded that iron-methionine chelate is more efficient than iron sulfate and 200 ppm iron supplementation as Fe-Met is recommended for maximum iron enrichment in broiler meat.

• Korean Journal of Head & Neck Oncology
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• v.3 no.1
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• pp.97-105
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• 1987

Chondrosarcoma of the head and neck is very rare tumor and this incidence was reported to be about 10% of all chondrosarcomas. Maxilla is the most common site of involvement and mandible, paransal sinus, nasal cavity and base of skull in that order. In general, chondrosarcoma has been known to be radioresistant, however since the Princess Margaret Hospital reported that it was radioresponsive tumor in 1980, the role of radiotherapy has been emphasized in terms of local control, especially in head and neck regions where complete excion is often difficult to achieve. The authors experienced 4 cases of chondrosarcoma of head and neck among all 29 chondrosarcoma patients from 1971 to 1985. The clinical and pathologic feature of this disease, it's treatment and prognosis were reviewed along with the literatures.

• Korean Journal of Plant Resources
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• v.20 no.3
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• pp.234-241
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• 2007

The present study was undertaken to classify and describe the riparian vegetation of the 6 river${\cdot}$stream of Korea Peninsula. As a result the vegetation was divided into nineteen communities. The vegetation units obtained in the present study were as follow: A: Salix koreensis community, B: Salix gracilistyla community, C: Robinia pseudo-acacia community, D: Amorpha fluticosa community, E: Brousonnetia papyrifera community, F: Phyllostachys bambusoides community, G: Rubus corchorifolius community, H: Phramities japonica community, I: Phramites communis community, J: Miscanthus sacchariflorus community, K: Miscanthus sinesis var. purpurascens community, L: Artemisia princeps var. orientalis community, M: Humulus japonicus community, N: Zoysia japonica community, O: Inperata cylindrica var. koenigii community, P: Agropyron tsukushiense var. transiens community, Q: Juncos effusus var. decipiens community, R: Rumex crispus community, S: Persicaria hydropiper community. The vegetation characteristics of riversides was recovered in the surveyed results according to river${\cdot}$stream basin, in other words, 10 communities in the upper river${\cdot}$stream riparian, 15 communities in the middle river${\cdot}$stream riparian, 10 communities in the down river${\cdot}$stream riparian. The Phragmites japonica community in the upper and Phragmites communis community in the down was analyzed by common community of the 6 river${\cdot}$stream riparian, respectively, but none in the middle. The standing profile of vegetation across 6 river${\cdot}$stream was seen stepwise Phragmites japonica community, Salix gracilistyla community, Miscanthus sinensis var. purpurascens community, shrub community by natural waterway in the upper, Salix gracilistyla community, Phragmites communis community, Rumex crispus community, Miscanthus sacchariflorus community, Humulus japonicus community in the middle, Phragmites communis community in the down. The differences of distributional featurs of vegetation emerged from the riparian of the 6 river${\cdot}$stream, but don't from the 6 river${\cdot}$stream.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.20
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• pp.100-106
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• 1975

Germinating ability of green seed in maturing period was investigated to develop the technics on generation shortening. Rapeseed did not germinate until 40 days after flowering (=DAF) in green seed and 50 DAF in dried seed. Effective germination to be utilized for generation shortening came on 55 DAF for dried seed by heating and on 75 DAF for green seed. The periodic time of seed dormancy in maturing period had two cycles and rest period was considered that it was different according to the ecological types of early, middle and late maturity.

• Toxicological Research
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• v.34 no.3
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• pp.221-229
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• 2018

Tenofovir nanoparticles are novel therapeutic intervention in human immunodeficiency virus (HIV) infection reaching the virus in their sanctuary sites. However, there has been no systemic toxicity testing of this formulation despite global concerns on the safety of nano drugs. Therefore, this study was designed to investigate the toxicity of Tenofovir nanoparticle (NTDF) on the liver and kidney using an animal model. Fifteen adult male Sprague-Dawley (SD) rats maintained at the animal house of the biomedical resources unit of the University of KwaZulu-Natal were weighed and divided into three groups. Control animals (A) were administered with normal saline (NS). The therapeutic doses of Tenofovir (TDF) and nanoparticles of Tenofovir (NTDF) were administered to group B and C and observed for signs of stress for four weeks after which animals were weighed and sacrificed. Liver and kidney were removed and fixed in formal saline, processed and stained using H/E, PAS and MT stains for light microscopy. Serum was obtained for renal function test (RFT) and liver function test (LFT). Cellular measurements and capturing were done using ImageJ and Leica software 2.0. Data were analysed using graph pad 6, p values < 0.05 were significant. We observed no signs of behavioural toxicity and no mortality during this study, however, in the kidneys, we reported mild morphological perturbations widening of Bowman's space, and vacuolations in glomerulus and tubules of TDF and NTDF animals. Also, there was a significant elevation of glycogen deposition in NTDF and TDF animals when compared with control. In the liver, there were mild histological changes with widening of sinusoidal spaces, vacuolations in hepatocytes and elevation of glycogen deposition in TDF and NTDF administered animals. In addition to this, there were no significant differences in stereological measurements and cell count, LFT, RFT, weight changes and organo-somatic index between treatment groups and control. In conclusion, NTDF and TDF in therapeutic doses can lead to mild hepatic and renal histological damage. Further studies are needed to understand the precise genetic mechanism.

• Journal of Embryo Transfer
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• v.22 no.1
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• pp.53-61
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• 2007

This study was investigated factors affecting the pregnancy rates after transfer of pronuclear microinjected embryos for the production of transgenic Korean black goats. Embryo transfer was carried out in 343 recipient Korean black goats from September 1999 to June 2000. Estrus was induced by the insertion of intravaginal progesterone devices $CIDR^(R)$ for 2 weeks. A single injection of 400 IU equine chorionic gonadotropin was administered at 48h before $CIDR^(R)$ removal to increase the proportion of does cycling and ovulation rate. Good quality embryos were prepared by microinjection of DNA into the pronuclei of fertilized goat oocyte and cultured in vitro. Pronuclear microinjected $1{\sim}8$ cell stage embryos were surgically transferred into the oviducts of the recipient at day 4 or 5 following $CIDR^(R)$ removal, and morula to blastocyst stage embryos were surgically transferred into uterus at day 9. Pregnancy was diagnosed by transrectal ultrasound scanning at $20{\sim}30d$ and 8 weeks following embryo transfer. The pregnancy rate was affected by several factors, such as estrus induction, the number of previous transfer, transfer site, stage of CL (corpus luteum), the number of recipient CL, stage of embryos and the number of transferred embryo. The pregnancy rate was significantly higher in recipients that came into estrus naturally than recipients that induced to come into estrus with $CIDR^(R)$(59.1% vs. 36.8%; P<0.05). The pregnancy rate was higher when the embryos were transferred into the left oviduct than transferred into the right oviduct (42.9% vs. 35.3%; P<0.05). The pregnancy rate of recipients with $CH_1$ (early) stage corpus hemorrhagicum in ovary was hi틴or than recipient with $CH_3$ (late) stage hemorrhagicum (47.5% vs. 17.9%; P<0.01). Higher pregnancy rates were obtained by transfer of 1-cell stage embryos into oviduct while late blastocysts (51.6% vs. 66.7%; P<0.01) into uterus. The pregnancy rates when 3 embryos were transferred to recipients were significantly higher than when 2 embryos we.e transferred (47.6% vs. 27.0%; P<0.05). Although there were no significant difference among the group, adhesion of reproductive organs, uterine size, ovulation rate of recipients, presence of large follicle and difficulty of transfer affected pregnancy rate of recipient. Higher pregnancy rates were obtained in the recipients with $8{\sim}15m$ diameter uterine horn as compared to the recipients with <5m diameter or >20mm diameter uterine hem (38.9%, 20% vs. 18.2%), in the recipients with large follicle in the ovulated ovary ipsilaterally (53.6% vs. 37.1%) and in the transfer which was carried out easily (39.2% vs. 27.8%, 0%). In conclusion, the high rate of pregnancy was achieved following transfer of pronuclear microinjected embryos when three or four 1-cell stage embryos were transferred into oviduct with $CH_1$ stage corpus hemorrhagicum in the ovary of recipient which came into estrus naturally.