• Asian-Australasian Journal of Animal Sciences
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• v.23 no.7
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• pp.863-866
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• 2010

Myostatin, which is also known as growth and differentiation factor 8 (GDF8), has been reported to act as a negative regulator of skeletal muscle development. Variation in the myostatin gene (MSTN) has been associated with variation in muscularity in certain "meaty" sheep breeds. Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) analysis was used to investigate allelic variation in the previously described g+6223G>A single-nucleotide polymorphism (SNP) in the 3' untranslated region (3' UTR) of MSTN. The sheep studied were 79 New Zealand (NZ) Romney lambs derived from a single sire heterozyous for g+6223G>A, which is in itself notable as this polymorphism has not been described previously in this breed. Allelic variation was observed to be associated with an abnormal gender ratio (p = 0.046) in the progeny. The presence of allele A was observed to have an effect (p<0.05) on birth weight, mean loin yield, proportion yield loin and total muscle yield. Allelic variation did not significantly affect mean shoulder yield, leg yield, proportion yield shoulder and proportion yield leg. This preliminary result suggests that while the A allele at MSTN g+6223 appears to improve some valuable traits in NZ Romney sheep, further research is required to understand if and how it may affect other traits.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.10
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• pp.1854-1862
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• 2006

The numerical methods of finding out the optimal position of optical phase conjugator (OPC) and the optimal fiber dispersions are prosed, which are able to effectively compensate overall channels in $8{\times}40Gbps$Gbps WDM system with non zero - dispersion shifted fiber (NZ-DSF) as an optical fiber. And BER characteristics in the system with two induced optimal parameters are compared with those in the system with the currently used mid-span spectral inversion (MSSI) in order to confirm the availability of the proposed methods. It is confirmed that the applying two induced optimal parameters into WDM system contribute to reduce power penalty to 4 times than that of WDM system with the conventional MSSI. Thus, the methods proposed in this research will be expected to alternate with the method of making a symmetrical distribution of power and local dispersion in real optical link which generates a serious problem if it was not made but it is the condition in the case of applying the OPC into multi-channels WDM system.

• Korean Journal of Environmental Biology
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• v.22 no.1
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• pp.213-219
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• 2004

The bacterial community of water stream, soil and guano in Gossi cave was examined by using PCR amplified the 16S rDNA-denaturing gradient gel electrophoyesis (DGGE). In this study, the genetic diversity and the similarity of bacterial community between open area and non - open area toy cave tour were investigated, and the seasonable variation pattern was compared each other. DGGE is attractive technique, as it sepayate same length dsDNA according to sequence variation typical 16S rDNA genes. The diversity and similarity of bacterial community in cave was analyzed by GC341f and PRUN518r primer sets foy amplification of V3 region of eubacteria 16S rDNA. The specific DGGE band profile of the cave water gives the possibility that the specific bacterial cell can be adapting to the specific cave environment and living in the cave. The DGGE band profiles of all samples with guano were compared and analyzed by image analyzer, in which mutual band profile was compared to be and the band intensity of guano was the highest. From these result, it is thought that the guano was main nutrient source and influenced on the community structure of the cave environment where is nutritionally limited. Pseudomonas sp. NZ060, Pseudomonas pseudoalcaligenes, uncultured Variovorax sp. and soli bacterium NS7 were identified to be on some sample from analysing DNA sequence of some DGGE band.

• Journal of Biomedical Engineering Research
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• v.18 no.4
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• pp.485-491
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• 1997

This paper describes the relationship between skin impedance signal, behavioral signal, and subjective evaluation depending on arousal level. Nz and reaction time had similar trend with mKSS level, but eyeblink rate was different from these two parameters. eye-blink rate increased slowly from mKSS level 1 to 5, and had high increasing rate at mKSS 7. But it showed steep descent at mKSS level 9. Each subject showed different eye-blink rates, but changing rates of EBR was similar at eachm KSS level. Therefore it suggests that rising rate of EBR can be used arousal level criterion. From the result of reaction time test. human performance was decreased rapidly above the mKSS level 5, and false positive and false negative data was observed above the mKSS level 3. It is desirable to give a subject some stimuli such as sound or aroma to rise arousal level between mKSS level 3 and mKSS level 5.

• Journal of Microbiology and Biotechnology
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• v.28 no.8
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• pp.1293-1298
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• 2018

Phosphomannomutase (ManB) converts mannose-6-phosphate (M-6-P) to mannose-1-phosphate (M-1-P), which is a key metabolic precursor for the production of GDP-D-mannose used for production of glycoconjugates and post-translational modification of proteins. The aim of this study was to express the manB gene from Escherichia coli in Lactococcus lactis subsp. cremoris NZ9000 and to characterize the encoded enzyme. The manB gene from E. coli K12, of 1,371 bp and encoding 457 amino acids (52 kDa), was cloned and overexpressed in L. lactis NZ9000 using the nisin-controlled expression system. The enzyme was purified by Ni-NTA column chromatography and exhibited a specific activity of 5.34 units/mg, significantly higher than that of other previously reported ManB enzymes. The pH and temperature optima were 8.0 and $50^{\circ}C$, respectively. Interestingly, the ManB used in this study had two substrate specificity for both mannose-1-phosphate and glucose-1-phosphate, and the specific activity for glucose-1-phosphate was 3.76 units/mg showing 70% relative activity to that of mannose-1-phosphate. This is the first study on heterologous expression and characterization of ManB in lactic acid bacteria. The ManB expression system constructed in this study canbe used to synthesize rare sugars or glycoconjugates.

• Journal of Ginseng Research
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• v.42 no.4
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• pp.412-418
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• 2018

Background: Ginsenoside Rg3(S) and compound K (C-K) are pharmacologically active components of ginseng that promote human health and improve quality of life. The aim of this study was to produce Rg3(S) and C-K from ginseng extract using recombinant Lactococcus lactis. Methods: L. lactis subsp. cremoris NZ9000 (L. lactis NZ9000), which harbors ${\beta}$-glucosidase genes (BglPm and BglBX10) from Paenibacillus mucilaginosus and Flavobacterium johnsoniae, respectively, was reacted with ginseng extract (protopanaxadiol-type ginsenoside mixture). Results: Crude enzyme activity of BglBX10 values comprised 0.001 unit/mL and 0.003 unit/mL in uninduced and induced preparations, respectively. When whole cells of L. lactis harboring pNZBglBX10 were treated with ginseng extract, after permeabilization of cells by xylene, Rb1 and Rd were converted into Rg3(S) with a conversion yield of 61%. C-K was also produced by sequential reactions of the permeabilized cells harboring each pNZBgl and pNZBglBX10, resulting in a 70% maximum conversion yield. Conclusion: This study demonstrates that the lactic acid bacteria having specific ${\beta}$-glucosidase activity can be used to enhance the health benefits of Panax ginseng in either fermented foods or bioconversion processes.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2006.05a
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• pp.361-364
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• 2006

The numerical methods of finding the optimal position of optical phase conjugator (OPC) and the optimal fiber dispersions are proposed, which are able to effectively compensate overall channels in $8\times40$ Gbps WDM system with non zero - dispersion shifted fiber (NZ-DSF) as an optical fiber. And BER characteristics in the system with two induced optimal parameters are compared with those in the system with the currently used mid-span spectral inversion (MSSI) in order to confirm the availability of the proposed methods. It is confirmed that the applying two induced optimal parameters into WDM system contribute to reduce power penalty to 4 times than that of WDM system with the conventional MSSI.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.12C
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• pp.1739-1747
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• 2004

In this paper, an efficient radix-4 systolic VLSI architecture for RSA public-key cryptosystem is proposed. Due to the simple operation of iterations and the efficient systolic mapping, the proposed architecture computes an n-bit modular exponentiation in n$^{2}$ clock cycles since two modular multiplications for M$_{i}$ and P$_{i}$ in each exponentiation process are interleaved, so that the hardware is fully utilized. We encode the exponent using Radix-4. SD (Signed Digit) number system to reduce the number of modular multiplications for RSA cryptography. Therefore about 20% of NZ (non-zero) digits in the exponent are reduced. Compared to conventional approaches, the proposed architecture shows shorter period to complete the RSA while requiring relatively less hardware resources. The proposed RSA architecture based on the modified Montgomery algorithm has locality, regularity, and scalability suitable for VLSI implementation.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.11-17
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• 2012

The Waikato River is New Zealand's longest River, though relatively small on international scales. It drains the central North Island and has New Zealand's largest lake (Lake Taupo) at its headwaters. The upper reaches have sustained flows fed by large aquifers which are recharged by rainfall events providing relatively constant river flows, whereas the lower reaches respond more directly to rainfall events having more peaky flows after rainfall and extreme low flows during dry periods. Consumptive allocation from the river is relatively low with only about 3% of the mean annual flow being allocated. However, more than seven times the river's flow is allocated for non-consumptive purposes before discharging to the Tasman Sea. The majority of this non-consumptive allocation is for hydro power generation and as cooling water at both thermal and geothermal power stations which produce up to 25% of New Zealand's electricity. The upper half of the river has been heavily modified with the construction of eight dams for power generation. This has resulted in a succession of cascading dams replacing the previously uncontrolled river. The Waikato River also provides drinking water for Auckland City (NZ's largest city) and Hamilton City (NZ's 4th largest city). In recent years there has also been considerable growth in water requirements for pasture irrigation to support the intensification of dairy farming in the catchment. Operators of the power stations are concerned that any further consumptive allocation will further reduce their ability to generate electricity. The Waikato Regional Council, who is charged with managing the river and allocation of water, has recently set new rules for managing the conflicting allocation demands on the Waikato River. This has resulted in an end to further allocation of water where it results in a loss of water for electricity generation from renewable resources (fresh water and geothermal water). The exception to this is the prioritisation of water for municipal supplies ahead of other consumptive uses such as industries and irrigators.

• Microbiology and Biotechnology Letters
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• v.47 no.3
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• pp.449-458
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• 2019

Hepatitis B treatments using immune therapy are gaining interest because of the improvements in dendritic cell performance for antigen presentation, which induces an appropriate immune response and raises patient survival rates. This research aims to produce a significant amount of the HBcAg antigen, which can induce an immune response and have a curative effect on HBV infection. In this study, the HBV subgenotype B3 of the HBcAg gene was used, which is dominant in Indonesia. Further, Lactococcus lactis bacteria was used as the host because of its safety and tightly regulated protein expression. The codon usage for the HBcAg gene was optimized to improve protein expression in L. lactis, which is important because a codon is not random between species. The HBcAg gene is attached to a pNZ8148 plasmid and transformed into the L. lactis NZ3900 expression host. The results confirm that a positive protein band (21 kDa) in two fractions of purified HBcAg was recognized by both western blotting and dot blot hybridization, even if the HBcAg optimized codon has higher GC contents than that suggested for L. lactis expression. Overall, this research strengthens the broad use of L. lactis bacteria for any protein expression, including higher protein expression of codon optimized HBcAg gene compared to non-optimized genes. Furthermore, the improvement in the codon optimization of the HBcAg gene significantly increases the total protein expression by 10-20%, and the expression level of the codon optimized HBcAg increases 1.5 to 3.2-times that of the native HBcAg.