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Ginsenosides analysis of New Zealand-grown forest Panax ginseng by LC-QTOF-MS/MS

  • Chen, Wei;Balan, Prabhu;Popovich, David G.
    • Journal of Ginseng Research
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    • v.44 no.4
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    • pp.552-562
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    • 2020
  • Background: Ginsenosides are the unique and bioactive components in ginseng. Ginsenosides are affected by the growing environment and conditions. In New Zealand (NZ), Panax ginseng Meyer (P. ginseng) is grown as a secondary crop under a pine tree canopy with an open-field forest environment. There is no thorough analysis reported about NZ-grown ginseng. Methods: Ginsenosides from NZ-grown P. ginseng in different parts (main root, fine root, rhizome, stem, and leaf) with different ages (6, 12, 13, and 14 years) were extracted by ultrasonic extraction and characterized by Liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Twenty-one ginsenosides in these samples were accurately quantified and relatively quantified with 13 ginsenoside standards. Results: All compounds were separated in 40 min, and a total of 102 ginsenosides were identified by matching MS spectra data with 23 standard references or published known ginsenosides from P. ginseng. The quantitative results showed that the total content of ginsenosides in various parts of P. ginseng varied, which was not obviously dependent on age. In the underground parts, the 13-year-old ginseng root contained more abundant ginsenosides among tested ginseng samples, whereas in the aboveground parts, the greatest amount of ginsenosides was from the 14-year-old sample. In addition, the amount of ginsenosides is higher in the leaf and fine root and much lower in the stem than in the other parts of P. ginseng. Conclusion: This study provides the first-ever comprehensive report on NZ-grown wild simulated P. ginseng.

Performance Improvement of 24X40 Gbps NRZ Channels in WDM System with 1,000 km NZ-DSF using Optimal Parameters of Optical Phase Conjugator

  • Lee, Seong-Real;Chung, Jae-Pil
    • Journal of information and communication convergence engineering
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    • v.5 no.2
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    • pp.164-170
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    • 2007
  • In this paper, the new method alternating with the method for forming the symmetrical distribution of power and local dispersion in high bit-rate WDM system with optical phase conjugator (OPC) is proposed. The proposed method is carried by finding out the optimal values of OPC position offset and fiber dispersion offset. It is assumed to be that NRZ-formatted 24-channels of 40 Gbps are simultaneously propagated in WDM system with non zero - dispersion shifted fiber (NZ-DSF) of 1,000 km. It is confirmed that the compensation extents of overall WDM channels are more improved by applying the induced optimal values into WDM system than those in WDM system with the conventional mid-span spectral inversion (MSSI) technique, and the searching procedure of the optimal values makes little difference of performance if the optimal value of one parameter related with another parameter. And, it is confirmed that the flexible design of WDM system with OPC is possible by effectiviely using by these optimal values. Thus, it is expected that the proposed method alternate with the forming method of the symmetrical distributions of power and local dispersion.

Variation in vowel duration depending on voicing in American, British, and New Zealand English

  • Cho, Hyesun
    • Phonetics and Speech Sciences
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    • v.8 no.3
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    • pp.11-20
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    • 2016
  • It is well known that vowels are shorter before voiceless consonants than voiced ones in English, as in many other languages. Research has shown that the ratio of vowel durations in voiced and voiceless contexts in English is in the range of 0.6~0.8. However, little work has been done as to whether the ratio of vowel durations varies depending on English variety. In the production experiment in this paper, seven speakers from three varieties of English, New Zealand, British, and American English, read 30 pairs of (C)VC monosyllabic words which differ in coda voicing (e.g. beat-bead). Vowel height, phonemic vowel length, and consonant manner were varied as well. As expected, vowel-shortening effects were found in all varieties: vowels were shorter before voiceless than before voiced codas. Overall vowel duration was the longest in American English and the shortest in New Zealand (NZ) English. In particular, vowel duration before voiceless codas is the shortest in New Zealand English, indicating the most radical degree of shortening in this variety. As a result, the ratio of vowel durations in varying voicing contexts is the lowest in NZ English, while American and British English do not show a significant difference each other. In addition, consonant closure duration was examined. Whereas NZ speakers show the shortest vowel duration before a voiceless coda, their voiceless consonants have the longest closure duration, which suggest an inverse relationship between vowel duration and closure duration.

Compensation for the Distorted $16{\times}40$ Gbps NRZ Channels in 1,000 km NZ-DSF WDM System Using MSSI with Optimal Parameters

  • Lee, Seong-Real
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.31 no.11A
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    • pp.1044-1052
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    • 2006
  • In this paper the optimum position of optical phase conjugator (OPC) and the optimal dispersion coefficients of filler sections in WDM system with the conventional mid-span spectral inversion (MSSI) are numerically induced and then applied into $16{\times}40$ Gbps WDM systems with 1,000km non zero - dispersion shifted fiber(NZ-DSF) in order to efficiently compensate for the distorted overall channels. It is confirmed that the compensation extents of overall WDM channels are more improved by applying the induced optimal parameters into WDM system than those in WDM system with the conventional MSSI. So it is expected to alternate with the forming method of the symmetrical distributions of power and local dispersion by applying these optimal parameters into the real WDM system, which generate a serious problem of applying the OPC into multi-channels WDM system if it is not formed. It is also confirmed that two optimal parameters depend on each other, but less related with the finding procedure. And, it will be possible to realize the flexible system design by applying the methods proposed in this paper into the real WDM system with OPC.

Expression of heterologous genes using the slpA promoter and signal sequence in Lactobacilli

  • Gang, Seung-Ha;Bok, Jin-Deok;Jo, Gwang-Geun;Jo, Jae-Sun;Choe, Yun-Jae
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.202-205
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    • 2000
  • A gene coding $endo-{\beta}$,-1, 4 glucanase from Actinomyces sp. KNG40 and phytase from Hansenula polymorpha were cloned into Esherichia coli JM101 by using E. coli/Lactobacillus shuttle vector pNZ3004 and pNZ123. The plasmid p3PS(1-4) and p123(1-4) have slpA promoter and slpA signal sequence. So, I constructed expression vectors, p3PS(1-4)Endo, phy and p123(1-4)Endo, phy. These constructed vector was transformed in target host Lactobacillus gasseri and reutri. These transformed host expressed endoglucanase and phytase as extracellular fraction. In the enzyme activity of the same vector, host L, gasseri was higher activity than L. reuteri. This indicates that L. gasseri recongnize promoter and signal sequence very well.

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A Study on Base Isolation Performance of MR Dampers Using Clipped-Optimal Control (Clipped-Optimal Control을 사용한 MR 감쇠기의 면진성능에 관한 연구)

  • 고봉준;이종세
    • Proceedings of the Computational Structural Engineering Institute Conference
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    • 2003.04a
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    • pp.529-536
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    • 2003
  • As large structures such as high-rise buildings and cable-stayed bridges become lighter and more flexible, the necessity of structural control for reducing excessive displacement and acceleration due to seismic excitation is increased. As a method to minimize seismic damages, various base isolation systems are adopted or considered for adoption. In this study, the seismic performance of MR dampers are studied and compared with that of the NZ system as a base isolation system. As the control algorithm of the MR damper, the clipped-optimal control(applied LQR method) is employed. A five-story building is modeled and the seismic performance of the two systems subjected to three different earthquakes is compared. The results show that the MR damper system can provide superior protection than the NZ system for a wide range of ground motions.

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A Study on the Seismic Performance of MR Fluid Dampers in Base-Isolated Structures (기초격리된 구조물에서 자기유동성 유체감쇠기의 면진성능에 관한 연구)

  • 이종세;도학용
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2002.09a
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    • pp.371-378
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    • 2002
  • The design concepts using vibration reduction techniques, or structural control, have been proposed recently to protect infrastructure against earthquakes. The magnetorheological(MR) fluid damper is one of the most promising new devices for structural vibration reduction because of its mechanical simplicity, high dynamic range, low power requirement, large force capacity and robustness. In this study, the seismic performance of MR devices are compared with that of NZ systems as an attempt to provide some data for improving seismic design techniques applied to structures. For nonlinear time domain analysis of a base isolation system, a six-story building model is considered as a numerical example. The ground acceleration data of El Centre 1940, Mexico City 1985 and Kobe 1995 earthquakes are used as seismic excitations. The results show that MR damper systems for outperform NZ systems in wide-ranging seismic excitations with respect to intensity and period characteristics.

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Seisminc Response of Base Isolated Structures with MR Dampers (MR 감쇠기를 적용한 기초격리구조물의 지진응답)

  • 고봉준;황인호;이종세
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2003.09a
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    • pp.505-512
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    • 2003
  • As large structures such as highrise buildings and cable-stayed bridges become lighter and more flexible, the necessity of structural control for reducing excessive displacement and acceleration due to seismic excitation is increased. As a means to minimize seismic damages, various base isolation systems are adopted or considered for adoption. In this study, the seismic performance of M dampers are studied and compared with that of the NZ system as a base isolation system As the control algorithm of the MR damper, the clipped-optimal control(applied LQR method) is employed. A five-story building is modeled and the seismic performance of the two systems subjected to three different earthquakes is compared. The results show that the M damper system can provide superior protection than the NZ system for a wide range of ground motions.

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The Flexible Design of 0.96 Tbps WDM System over 1,000km NZ-DSF by Using Optimal Parameters of Optical Phase Conjugator (광 위상 공액기의 최적 파라미터를 이용한 1,000km NZ-DSF를 갖는 0.96 Tbps WDM 시스템의 유연한 설계)

  • Lee, Seong-Real;Doh, Kyu-Bong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.7A
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    • pp.657-665
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    • 2007
  • In this paper, it is investigated that the limitation due to the asymmetry of optical power and chromatic dispersion with respect to optical phase conjugator (OPC) for compensating optical signal distortion of WDM channels in mid-span spectral inversion (MSSI) technique is overcame by using OPC position offset and optimal dispersion coefficients of fiber sections, which depend on OPC position offset. It is confirmed that overall WDM channels are efficiently compensated by applying the optimal parameter values obtained from the proposed method into 24 channels ${\times}\;40\;Gbps$ WDM system with non zero - dispersion shifted fiber (NZ-DSF) of 1,000 km, such as power penalties of inter-channel are reduce to almost 3.5 dB from the infinite value. It is also confirmed that the flexible design of WDM system with OPC is possible by using the optimal parameters, in which OPC is placed at ${\pm}15\;km$ from 500 km for efficiently compensating overall channels.