• Mycobiology
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• 제37권2호
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• pp.114-120
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• 2009

Streptomyces albidoflavus C247 was isolated from the soil of the Gyeongsan golf course in Korea. Physiological, biochemical and 16S rDNA gene sequence analysis strongly suggested that the isolate belonged to Streptomyces albidoflavus. Preliminary screening revealed that the isolate was active against fungi and bacteria. Self-directing optimization was employed to determine the best combination of parameters such as carbon and nitrogen source, pH and temperature. Nutritional and culture conditions for the production of antibiotics by this organism under shake-flask conditions were also optimized. Maltose (5%) and soytone (5%) were found to be the best carbon and nitrogen sources for the production of antibiotics by S. albidoflavus C247. Additionally, 62.89% mycelial growth inhibition was achieved when the organism was cultured at $30^{\circ}C$ and pH 6.5. Ethyl acetate (EtOAc) was the best extraction solvent for the isolation of the antibiotics, and 100 ${mu}$/ml of EtOAc extract was found to inhibit 60.27% of the mycelial growth of Rhizoctonia solani AG2-2(IV) when the poison plate diffusion method was conducted.

• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.313-319
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• 2007

The nutritional medium requirement for biosurfactant production by Bacillus licheniformis K51 was optimized. The important medium components, identified by the initial screening method of Plackett-Burman, were $H_3PO_4,\;CaCl_2,H_3BO_3$, and Na-EDTA. Box-Behnken response surface methodology was applied to further optimize biosurfactant production. The optimal concentrations for higher production of biosurfactants were (g/l): glucose, $1.1;NaNO_3,\;4.4;MgSO_4{\cdot}7H_2O,\;0.8;KCl,\;0.4;CaCl_2,\;0.27;H_3PO_4,\;1.0ml/l;\;and\;trace elements\;(mg/l):H_3BO_3,\;0.25;CuSO_4,\;0.6;MnSO_4,\;2.2;Na_{2}MoO_4,\;0.5;ZnSO_4,\;6.0;FeSO_4,\;8.0;CoCL_2,\;1.0;$ and Na-EDTA, 30.0. Using this statistical optimization method, the relative biosurfactant yield as critical micelle dilution (CMD) was increased from $10{\times}\;to\;105{\times}$, which is ten times higher than the non-optimized rich medium.

• 한국식품영양과학회지
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• 제29권2호
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• pp.307-311
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• 2000

The purpose of this study was to identify excellent immune-enhancing substance from Korean wheats(Eunpa, Gueru, Alchan, Topdong, Suwon 267, Gobun) compared with imported ones(Australian standard white, ASW; Dark northern spring, DNS). Phagocytic activities of PBS (phosphate buffered saline, pH 7.4) and EA(ethanol-acetic acid) extracts from the wheats were determined using mouse macrophage J774 cell line. In order to set the optimal experimental condition up, the cultured cells were tested in varying experimental conditions. About two to five times higher phagocytic activity was shown in EA extract of Korean wheats compared to that of imported wheats. PBS extracts of wheats did not show increased phagocytic activity compared to control that did not add any extract. The EA extract of Gobun wheat showed the highest phagocytic activity. From the experiment we found that the optimal experimental condition was shown in two hours of reaction time and 0.05mg amout of EA extract added to J774 cells.

• Journal of Nutrition and Health
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• 제49권5호
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• pp.313-322
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• 2016

본 연구는 용인시에 거주하는 노인들을 대상으로 간이인지기능검사 (MMSE-DS)와 영양건강위험도 조사 (NSI DETERMINE checklist) 및 식품섭취실태를 통하여 인지기능 저하와 영양 식생활 실태와의 관련성을 살펴보고자 하였다. 1) 전체 조사대상 노인 (n = 409)의 평균 나이는 73.3세였고, 남자가 32.3%, 여자가 67.7%이었다. 인지기능 저하로 판정된 사람은 105명으로 대상자의 25.7%가 인지기능 저하로 판정되었으며, 성별에 따른 인지기능 정도의 차이는 없었다. 2) 조사대상 노인들의 운동 빈도는 유의차는 없었으나, 인지기능저하군에서 정상군보다 '운동을 거의 하지 않는다'는 비율은 더 높고, '거의 매일 운동을 한다'는 비율은 낮은 편이었다. 1일 평균 운동 시간은 인지기능저하군이 정상군보다 하루에 1~2시간씩 운동을 하는 사람들은 더 적고, 30분~1시간씩 운동을 하는 사람들은 더 많아서 총 운동시간이 적은 것으로 나타났다. 3) NSI DETERMINE 검사를 이용한 영양건강 위험도는 저위험군 270명 (66%), 중위험군 90명 (22%), 고위험군 49명 (12%)이었으며, 인지기능저하군에서 정상군보다 영양건강의 중위험군과 고위험군의 비율이 유의적으로 높았다. 4) 조사대상자의 평균 에너지 섭취량은 1,528.6 kcal로 에너지 필요추정량 (EER)의 84.4%를 섭취하였고, 인지기능저하군에서 정상군보다 다불포화지방산을 유의하게 적게 섭취하였다. 또한, 인지기능저하군은 티아민, 리보플라빈, 철을 평균필요량 미만으로 섭취하는 사람의 비율이 정상군보다 유의적으로 많았다. 5) 식품섭취빈도조사에서 조사한 63개 식품 중 조사대상자의 인지기능에 따라 유의차를 보인 식품은 6가지로 쌀밥과 과자류의 섭취빈도는 인지기능저하군에서 정상군보다 유의하게 높았고, 고등어, 고추, 귤과 수박의 섭취빈도는 인지기능저하군에서 유의하게 낮았다. 본 연구를 통하여 인지기능이 저하된 노인의 경우 정상노인보다 운동 빈도와 운동 시간이 적고, 영양건강위험도는 높으며, 불포화지방산의 섭취가 적고, 티아민, 리보플라빈, 철 등이 정상군보다 더 부족할 가능성이 높음을 확인하였다. 또한, 인지기능저하군에서 쌀밥과 과자류의 섭취는 더 많으나, 고등어, 고추, 귤, 수박의 섭취 빈도는 더 적은 것으로 나타났다. 앞으로 인지기능 손상을 지연시켜 개인의 건강 장수와 사회적 의료비용 절감을 가져오기 위해서는 인지기능 관련 식품들이 어떤 기전으로 인지기능에 영향을 미치는 가에 대한 후속 연구가 필요하다고 하겠다.

• 대한영양사협회학술지
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• 제8권3호
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• pp.217-226
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• 2002

The purpose of this study is to investigate initial nutritional status of stomach cancer patients. The study subjects were 88 patients with stomach cancer admitted at Kosin University Gospel Hospital in Busan. We assessed the initial nutritional status by anthropometric, biochemical and dietary intake data along with subjective global assessment(SGA). The results are as follows. 1. The mean age, weight, height, triceps skinfold thickness, mid arm circumference, and mid arm muscle circumference of the subjects were 55.9$\pm$11.0years, 60.0$\pm$9.8kg, 162.4$\pm$8.5cm, 10.9$\pm$5.7mm, 26.8$\pm$3.8cm, and 23.4$\pm$3.5cm respectively. The mean body mass index was within the normal range, with 22.7$\pm$2.8kg/m2, while 15.4% of the patients was underweight. The result shows that body fat mass and body protein mass of the patients with stomach cancer were decreased. 2. The mean biochemical data of the subjects were 4.0$\pm$0.5g/dl for albumin, 174.7$\pm$41.9mg/dl for cholesterol, 107.6$\pm$57.2mg/dl for triglyceride, 92.1μg/dl for Zn, 　297.0$\pm$103.1mg/dl for transferrin, 1980.0$\pm$0.8$mm^3$ for total lymphocyte count. 3. Daily energy intake was 1997.8$\pm$579.3kcal. And the ratio of carbohydrate, protein, and lipid to energy intake was 72:14:14. 4. The patients were divided into three groups according to SGA performed by an observer. Group A(well nourished) was 55.7% with 49 patients, Group B(moderately malnourished) was 22.7 % with 20 patients, and Group C(severely malnourished) was 21.6 % with 19 patients. The three groups showed a significant difference in body weight(p<0.01), 1 month weight loss %(p<0.001), 6 months weight loss %(p<0.001), body mass index(p<0.01), and mid arm circumference(p<0.05), albumin(p<0.01), energy intake(p<0.05) as well as carbohydrate intake(p<0.05). From these results, it may be concluded that SGA can be used as a nutrition screening tool, and comprehensive nutrition assessment is desirable for those malnourished.

• Clinical and Experimental Pediatrics
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• 제49권1호
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• pp.18-23
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• 2006

목 적 : 영양상태, 환경 오염원에 노출, 질병상태의 한 검진 방법으로서 모발 미네랄검사의 타당성에 대해 아직 많은 이견이 있으며 아직 국내 정상 소아의 참고치는 연구된 바 없다. 이에 저자들은 건강한 아동들의 모발 미네랄 함량의 정상 참고치를 산출하고 그 특성을 알아보고자 하였다. 방 법 : 서울시의 북부지역에 거주하는 3-12세 사이의 질병이 없는 건강한 정상 아동 223명(남아 110명, 여아 113명, 평균 연령 $8.8{\pm}2.2$세)을 대상으로 모발을 채취하여 유도결합플라즈마질량분석기(ICP-MS)를 통해 영양 미네랄 11가지(나트륨, 마그네슘, 인, 칼륨, 칼슘, 크롬, 망간, 철, 구리, 아연, 셀레늄)와 독성 미네랄 6가지(알루미늄, 비소, 카드뮴, 바륨, 수은, 납)의 함량을 측정하고 이를 분석하였다. 결 과 : 칼슘 및 마그네슘의 농도는 여아에서 남아보다 높았고 카드뮴, 납 및 크롬의 농도는 남아에서 여아보다 높았다. 아연, 칼슘, 마그네슘, 구리 및 수은은 연령이 증가할수록 모발 중 함량이 증가하는 상관관계를 보였다. 한국 아동의 모발 미네랄 함량의 참고치를 외국과 비교하였을 때 우리나라 아동은 아연치가 외국에 비해 낮은 경향을 보였다. 결 론 : 본 연구를 통해 국내 정상 아동의 모발 미네랄 함량의 기준치가 제시되었으며 본 연구에서 제시된 참고치가 한국 아동들의 여러 가지 질병과 모발 내 미네랄 함량과의 관계를 연구하는 데 유용한 기초 자료로 쓰일 것이라 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권2호
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• pp.783-786
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• 2015

Background: Utilizing geriatric screening tools for the identification of vulnerable older patients with cancer is important. The aim of this study is to evaluate the hospitalization risk of elderly hematologic cancer patients based on geriatric assessment and laboratory parameters. Materials and Methods: In this cross sectional study 61 patients with hematologic malignancies, age 65 years and older, were assessed at a hematology outpatient clinic. Standard geriatric screening tests; activities of daily living (ADL), instrumental activities of daily living (IADL), Mini Nutritional Assessment (MNA), Mini Mental State Examination (MMSE), timed up and go test (TUG), geriatrics depression scale (GDS) were administered. Demographic and medical data were obtained from patient medical records. The number of hospitalizations in the following six months was then recorded to allow analysis of associations with geriatric assessment tools and laboratory parameters. Results: The median age of the patients, 37 being males, was 66 years. Positive TUG test and declined ADL was found as significant risk factors for hospitalization (p=0.028 and p=0.015 respectively). Correlations of hospitalization with thrombocytopenia, vitamin B12 and folic acid deficiency were statistically significant (p=0.004, p=0.011 and p=0.05 respectively). Conclusions: In this study, geriatric conditions which are usually unrecognized in a regular oncology office visit were identified. Our study indicates TUG and ADL might be use as predictive tests for hospitalization in elderly oncology populations. Also thrombocytopenia, and vitamin B12 and folic acid deficiencies are among the risk factors for hospitalization. The importance of vitamin B12 and folic acid vitamin replacement should not be underestimated in this population.

• 한국식품과학회지
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• 제42권4호
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• pp.390-397
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• 2010

Residual solvents in foods are defined as organic volatile chemicals used or produced in manufacturing of extracts or additives, or functional foods. The solvents are not completely eliminated by practical manufacturing techniques and they also may become contaminated by solvents from packing, transportation or storage in warehouses. Because residual solvents have no nutritional value but may be hazardous to human health, there is a need to remove them from the final products or reduce their amounts to below acceptable levels. The purpose of this study was to develop and evaluate an analytical method for the screening of residual solvents in health functional foods. Furthermore, the aim of this study was to constitute a reasonable management system based on the current state of the market and case studies of foreign countries. Eleven volatile solvents such as MeOH, EtOH, trichloroethylene and hexane were separated depending on their column properties, temp. and time using Gas Chromatography (GC). After determining the GC conditions, a sample preparation method using HSS (Head Space Sampling) was developed. From the results, a method for analyzing residual solvents in health functional foods was developed considering matrix effect and interference from the sample obtained from the solution of solvents-free health functional foods spiked with 11 standards solutions. Validation test using the developed GC/HSS/MS (Mass Spectrometry) method was followed by tests for precision, accuracy, recovery, linearity and adequate sensitivity. Finally, examination of 104 samples grouped in suits was performed by the developed HSS/GC/MS for screening the solvents. The 11 solvents were isolated from health functional foods based on vapor pressure difference, and followed by separation within 15 minutes in a single run. The limt of detection (LOD), limit of quantification (LOQ), recovery and coefficient of variation (C.V.) of these compounds determined by the HSS/GC/MS were found to be 0.1 pg/mL, 0.1-125 pg/g, 51.0-104.6%, and less than 15%, respectively. Using the developed HSS/GC/MS method, residual solvent from 16 out of 104 health functional products were detected as a EtOH. This method therefore seems t o be a valuable extension ofanalytical method for the identification of residual solvents in health functional food.

• 대한간호학회지
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• 제38권1호
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• pp.122-130
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• 2008

Purpose: This study aimed to identify the associated factors of the perceived health status of the elderly in Korea and to provide basic data for developing nursing interventions for the elderly's health management. Methods: This study used a descriptive correlational research design. The subjects of this study were 335 elderly people over 60 yr living in an urban city. Data were collected through personal interviews using questionnaires from September 2006 to March 2007. Empirical indicators of perceived health status were measured by SF-36, nutritional screening initiative (NSI), activities of daily living (ADL) Index, and instrumental activities of daily living (IADL) scale. The collected data were analyzed by descriptive statistics, Pearson's correlation, and hierarchical regression. Results: The mean age of the subjects was 72.8 and 57.0% of subjects were female. 41.5% of variance in physical health was explained by nutrition, ADL, IADL, and the number of years attending school. Among them the most important factor was ADL. 13.2% of variance in mental health was explained the number of family, nutrition, and IADL. Among them the most important factor was nutrition. Conclusion: It is necessary to develop supportive interventions for improving the perceived health status of elderly people by considering the most important factors shown in this study.

• 한국지역사회생활과학회지
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• 제20권1호
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• pp.19-32
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• 2009

The purpose of this study was to examine household food insecurity and the associations of food insecurity with socioeconomic characteristics, food behaviors, health status and nutrient intake among elderly people in a rural area. This study included 191 elderly people (mean age = $72.0{\pm}7.4$) in Jeollabuk-do, Jangsu-gun. House food insecurity was measured using an adapted version of the USDA short form household food insecurity scale, and nutrient intake measured by a 24-hour dietary recall. Only 15.2% of the subjects were in food-insecure households, 84.8% of the elderly households were food-secure. The food-insecure households were significantly lower in the number of family, frequency of shopping, and perceived health status than the food-secure households. In addition, they had serious tooth problems and NSI (Nutrition Screening Index) scores. Food-insecure households were significantly lower in most areas of nutrient intake (energy, protein, fat, Ca, P, Fe, K, Na, Zn, Vit. $B_2$, niacin, Vit. E), and also lower in nutrient intake compared to dietary reference intake (energy, protein, P, Fe, K, Na, Vit. $B_1$, Vit. $B_2$, niacin, Vit. E). These results suggest that the household food insecurity measures used in this study were an important indicator of nutritional well-being among elderly persons in the rural area. Food insecurity should be considered an important issue to public health and food service programs should be expanded for food-insecure households in rural areas.