Browse > Article
http://dx.doi.org/10.4489/MYCO.2009.37.2.114

Isolation, Identification and Optimal Culture Conditions of Streptomyces albidoflavus C247 Producing Antifungal Agents against Rhizoctonia solani AG2-2  

Islam, Rezuanul (Department of Biotechnology, Daegu University)
Jeong, Yong-Tae (Department of Biotechnology, Daegu University)
Ryu, Yeon-Ju (Department of Bioindustry, Daegu University)
Song, Chi-Hyun (Department of Biotechnology, Daegu University)
Lee, Yong-Se (Department of Bioindustry, Daegu University)
Publication Information
Mycobiology / v.37, no.2, 2009 , pp. 114-120 More about this Journal
Abstract
Streptomyces albidoflavus C247 was isolated from the soil of the Gyeongsan golf course in Korea. Physiological, biochemical and 16S rDNA gene sequence analysis strongly suggested that the isolate belonged to Streptomyces albidoflavus. Preliminary screening revealed that the isolate was active against fungi and bacteria. Self-directing optimization was employed to determine the best combination of parameters such as carbon and nitrogen source, pH and temperature. Nutritional and culture conditions for the production of antibiotics by this organism under shake-flask conditions were also optimized. Maltose (5%) and soytone (5%) were found to be the best carbon and nitrogen sources for the production of antibiotics by S. albidoflavus C247. Additionally, 62.89% mycelial growth inhibition was achieved when the organism was cultured at $30^{\circ}C$ and pH 6.5. Ethyl acetate (EtOAc) was the best extraction solvent for the isolation of the antibiotics, and 100 ${mu}$/ml of EtOAc extract was found to inhibit 60.27% of the mycelial growth of Rhizoctonia solani AG2-2(IV) when the poison plate diffusion method was conducted.
Keywords
Antifungal agent; Rhizoctonia solani AG2-2(IV); Streptomyces albidoflavus C247;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Atlas, R. M. and Bartha, R. 1998. Fundamentals and applications. In: Microbial ecology. 4th edition, pp. 174-217. Benjamin/Cummings Science Publishing, New York
2 Marten, P., Bruckner, S., Minkwitz, A., Luth, P. and Bergm G. 2001. RhizovitR: Impact and formulation of a new bacterial product. In: Koch, E., Leinonen P. (Eds.), Formulation of Microbial Inoculants: Proceedings of a meeting held in Braunschweig, Germany, pp. 78-82. COST Action 830/Microbial inoculants for agriculture and environment, Germany.
3 MTCC. 1998. Actinomycetes. In: Isolation, Screening, Identification and; 1; Gene Cloning of Streptomyces. Laboratory Manual. pp.94, Institute of Microbiology and Technology, Chandigarh, India.
4 Bentley, S. D., Chater, R. K. F., Cerdeno-Tarraga, A. M., Challis, G. L., Thomson, N. R., James, K. D., Harris D. E., Quail, M. A., Kieser, H., Harper, D., et al. 2002. Complete genome sequence of the model actinomycete. Streptomyces coelicolor A3(2). Nature 417:141-147.   DOI   ScienceOn
5 Iwai, Y., Awaya, J., Kesado, T., Yamada, H., Omura, S. and Hata, T. 1973. Selective fermentation of cerulenin by Cephalosporin caeruleus. J. Ferment. Technol. 51:575.
6 Landa, B. B., Hervas, A., Bethiol, W. and Jimenez-Diaz, R. M. 1997. Antagonistic activity of bacteria from chickpea rhizosphere against Fusarium oxysporum f. sp. ciceris. Phytoparasitica 25:305-318.   DOI   ScienceOn
7 Liu, Q., Wu, Y. H. and Yu, J. C. 2004a. Screening for antagonistic actionmyces isolates from greenhouse soil in northeast China. Soil 36:573-575 (in Chinese).
8 Okami, Y. and Hotta, K. 1988. Search and discovery of new antibiotics. In: Good fellow M, Williams ST, Mordarski M, editors. Actinomycetes in biotechnology, pp. 33-67. Academic Press Inc., New York
9 Nolan, R. and Cross, T. 1998. Isolation and screening of actinomycetes. In: Good fellow M, Williams ST, Mordarski M, editors. Actinomycetes in biotechnology. Academic Press, London
10 Locci, R. 1989. Streptomyces and related genera In Bergey's manual of systematic bacteriology, 4th edition, pp. 2451-2508. Baltimore: Williams & Wilkins Company, New York.
11 Woese, C. R. 1987. Bacterial evolution. Microbiol. Rev. 51:221-271.
12 Sosio, M., Bossi, E., Bianchi, A. and Donadio, S. 2000. Multiple peptide synthetase gene clusters in actinomycetes. Mol. Gen. Genet. 264:213-221.   DOI   ScienceOn
13 Maniatis, T., Tritsch, E. F. and Sambrook, J. 1989. Molecular cloning. In: A laboratory manual, 2nd edition. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
14 Pandey, D. K., Tripathi, N. N., Tripathi, R. O. and Dixit, S. N. 1982. Fungitoxic and phyototoxic properties of essential oil of Phylis sauvolensis. Pfkrankh Pfschuz 89:334-346.
15 Mincer, T. J., Jensen, P. R., Christopher, A. K. and Fenical, W. 2002. Widespread and persistent populations of a major new marine actinomycetes taxon in ocean sediments. Appl. Environ. Microb. 68:5005-5011.   DOI   ScienceOn
16 Sujatha, P., Bapi Raju, K. V. V. S. N. and Ramana, T. 2005. Studies on a new marine Streptomycete BT-408 producing polyketide antibiotic SBR-22 effective against methicillin resistant Staphylococcus aureus. Microbiol. Res. 160:119-126.   DOI   ScienceOn