• Molecules and Cells
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• v.21 no.1
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• pp.153-160
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• 2006

Virus-induced gene silencing (VIGS) is an attractive reverse-genetics tool for studying gene function in plants. We showed that silencing of a phytoene desaturase (PDS) gene is maintained throughout TRV-PDS-inoculated tomato plants as well as in their flowers and fruit and is enhanced by low temperature ($15^{\circ}C$) and low humidity (30%). RT-PCR analysis of the PDS gene revealed a dramatic reduction in the level of PDS mRNA in leaves, flowers and fruits. Silencing of PDS results in the accumulation of phytoene, the desaturase substrate. In addition, the content of chlorophyll a, chlorophyll b and total chlorophyll in the leaves of PDS-silenced plants was reduced by more than 90%. We also silenced the LeEIN2 gene by infecting seedlings, and this suppressed fruit ripenning. We conclude that this VIGS approach should facilitate large-scale functional analysis of genes involved in the development and ripening of tomato.

• Journal of Microbiology and Biotechnology
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• v.7 no.3
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• pp.200-203
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• 1997

Bacteria isolated from ultrapure water made by a high-purity water manufacturing system in a semiconductor manufacturing plant were classified into two groups which either grew in diluted nutrient broth medium (oligotrophic bacteria) or could not grow (copiotrophic bacteria). The nutritional flexibility of oligotrophic and copiotrophic bacteria was investigated. The oligotrophic bacteria were shown to be able to utilize a significantly broader range of organic substrates than the copiotrophic bateria. This finding substantiates the hypothesis that nutritional flexibility is adaptive for oligotrophic bacteria.

• Journal of The Korean Society of Clinical Toxicology
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• v.19 no.2
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• pp.93-99
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• 2021

Purpose: Nutritional status and support in critically ill patients are important factors in determining patient recovery and prognosis. The aim of this study was to analyze the early nutritional status and the methods of nutritional support in critically ill patients with acute poisoning and to evaluate the effect of nutritional status on prognosis. Methods: A retrospective study was conducted in tertiary care teaching hospital from January 2018 to December 2020. in an emergency department of university hospital, 220 patients who were stayed more than 2 days of poisoning in intensive care unit were enrolled. Results: 155 (70.5%) of patients with acute poisoning had low-risk in nutritional risk screening (NRS). Patients with malignancy had higher NRS (low risk 5.2%, moderate risk 18.5%, high risk 13.2%, p=0.024). Patients of 91.4% supplied nutrition via oral route or enteral route. Parenteral route for starting method of nutritional support were higher in patients with acute poisoning of herbicide or pesticide (medicine 3.2%, herbicide 13.8%, pesticide 22.2%, p=0.000). In multivariate logistic regression analysis, herbicide or pesticide intoxication, higher risk in NRS and sequential organ failure assessment over 4.5 were affecting factor on poor recovery at discharge. Conclusion: NRS in patients intoxicated with herbicide or pesticide were higher than that in patients intoxicated with medicine intoxication. Enteral nutrition in patients intoxicated with herbicide or pesticide was less common. Initial NRS was correlated with recovery at discharge in patient with intoxication. It is expected to be helpful in finding patients with high-risk nutritional status in acute poisoning patients and establishing a treatment plan that can actively implement nutritional support.

• Journal of the East Asian Society of Dietary Life
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• v.23 no.2
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• pp.119-132
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• 2013

The aim of this study was to identify sushi choices underlying food-related lifestyle and to study the sushi attribute. The Food Choice Questionnaire (FCQ), the most popular tool for lifestyle research, which measures the relative importance of nine factors important in food choice, and food-related lifestyle was administered to 200 Americans (over 20 years of age) residing in Korea. From an analysis of food-related lifestyle through factor analysis, four factors emerged: health, taste, economy, and convenience. The Food Choice Questionnaire (FCQ) structure was verified using factor analysis, and eight factors were found: mood, natural content, health, sensory appeal, convenience, weight control, familiarity and price. These data are important in demonstrating the different motives underlying sushi choice. Moreover, the results suggest that an American's food-related lifestyle is considerably different in consumer behavior and sushi choice. Further research is needed to test our hypotheses.

• Journal of Korean Academy of Nursing
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• v.44 no.6
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• pp.708-716
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• 2014

Purpose: The purpose of this study was to evaluate the nutritional status of low-income urban elders by diversified ways, and to analyze the risk factors for malnutrition. Methods: The participants in this study were 183 low-income elders registered at a visiting healthcare facility in a public health center. Data were collected using anthropometric measurements, and a questionnaire survey. For data analysis, descriptive statistics, ${\chi}^2$-test, t-test, Fisher's exact test, multiple logistic regression analysis were performed using SPSS 20.0. Results: Regarding the nutritional status of low-income elders as measured by the Mini Nutritional Assessment (MNA), 10.4% of the elders were classified as malnourished; 57.4% as at high risk for malnutrition; and 32.2% as having normal nutrition levels. The main factors affecting malnutrition for low-income elders were loss of appetite (OR=3.34, 95% CI: 1.16~9.56) and difficulties in meal preparation (OR=2.35, 95% CI: 1.13~4.88). Conclusion: In order to effectively improve nutrition in low-income urban elders, it is necessary to develop individual intervention strategies to manage factors that increase the risk of malnutrition and to use systematic approach strategies in local communities in terms of a nutrition support system.

• Journal of Nutrition and Health
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• v.34 no.7
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• pp.770-777
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• 2001

This study investigated nutritional status and eating behaviors among 59 nephritic patients with mild kidney malfunction in Korea. Nutritional status was measured by blood analysis and 1-3 day dietary recall and records, and eating behaviors were assessed by a questionnaire. Mean Body Mass Index(BMI) was within the normal range, while 21% and 14% of the patients were underweight and overweight, respectively. They received nutrition information mainly from doctors, nurses and mass media, but rarely from nutrition professionals. A quarter of patients skipped breakfast at least 3 times per 12% of the patients, respectively. Energy intake of 83% of the patients was less than the recommended level. Protein intakes of 56% of them were either under or over the recommended levels. Patients had low vitamin B$_2$ and calcium(<75% of the RDA) and excessive phosphorus(138% RDA) and vitamin C(170% RDA). Major food sources to absolute nutrient intakes were similar to those for the average Koreas, except for milk. Milk intake was low in our patients. Dietary quantity and quality were associated positively with BMI, albumin, and HDL-cholesterol and negatively with triglycerides and BUN. Results of this study indicate low nutritional status of the patients and, in turn a need for conducting nutritional education or counselling at regular at regular basis for the nephritic patients with mild kidney malfunction.

• Journal of Nutrition and Health
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• v.30 no.3
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• pp.326-335
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• 1997

In the Health Promotion Law proclaimed on January 1995, nutritional improvement at national level was emphasized and designated as one of the jobs to be carried out by local governmnets. With such a situational necessity, we conducted a dietary survey along with an anthropometric measurement, biochemical assessment and questionnaire analysis on general characteristics of the students from 3 junior higher schools in Seoul area. About 300 students had participated in the study and the data from only 139 students, 28 boys and 111 girls, with complete report of dietary intake were subjected to analysis, comparison and discussion. Fasting blood samples were drawn and analyzed for hemoglobin, hematocrit and total cholesterol. Dietary intake was monitored by 1-day 24hr recall +2-day food record. In general, the average intake of nutrients for most of the subjects were above RDA for korean of that age except for 2 nutrients namely, vitamin A and calcium, of which average intake corresponded to 46-69% of RDA. In addition to this kind of nutritional imbalance, there were several other factors of nutritional problems such as skipping breakfast, overeating at dinner and frequent eating of snacks. As the best countermeasure for these kinds of nutritional problems, more detailed campaign and prractical nutrition education for these adolescents are necessary. Only through proper education and guidance for them, the healthy and intellectual man power could be guaranteed for the forthcoming 21st century.

• Asian Oncology Nursing
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• v.5 no.2
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• pp.87-96
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• 2005

Purpose: The purpose of this study was to identify the relationship between fatigue and nutritional status in gastric cancer Design. A correlational and crossectional study design was used. Method: Fatigue was measured using the Revised Piper Fatigue Scale. The parameters for nutritional status consisted of BMI, Hb, Hct, total protein, albumin and lymphocyte count. Result: The mean score of fatigue was $94.31{\pm}22.08(mean\;average\;4.48{\pm}1.00)$. The subdimensional scores of fatigue revealed $34.15{\pm}8.76$ for behavior/severity, $22.00{\pm}7.93$ for cognitive/mood, $21.67{\pm}5.56$ for sensory, and $20.81{\pm}6.69$ for affective. And the level of BMI, Hb, & Hct were under the normal ranges. In the ancillary analysis, there was a significant difference on the fatigue scores by the groups of present diet(F=3.343, P=.026). Age, types of treatments, period after diagnosis and weight change were related to nutritional status. There was a significant negative correlation between fatigue and nutritional status(r=-.371, P=.004). Conclusion: Considering the relationship between fatigue and nutritional status, nurses can identify the risk group most vulnerable to fatigue and malnourishment in order to provide appropriate interventions for them.

• Korean Journal of Community Nutrition
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• v.5 no.2
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• pp.343-351
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• 2000

The Korean government enacted the National Health Promotion Act in January, 1995 and proclaimed its regulations and rules in September 1995, which became the basis of the national health policy. The health promotion programs consist of education for health, prevention of diseases, improvement of nutrition, and practice of healthy life style as defined in that Act. The Community Health Act was amended in 1995, which included implementing nutrition services in community health centers. The purpose of this report is to summerize the nutrition services conducted in 32 community health centers. the main nutritional activities were as follows : 1) nutritional guidance by counseling and education for pregnant or lactating women, infants, preschool children, and those with chronic diseases, 2) collection, analysis, and interpretation of data collected from the community, on background conditions and target population for the assessment of community needs, 3) evaluation of nutritional status of population in the community 4) nutritional guidance for mass feeding in different institution including schools and welfare institutions. In order to meet the government's expectations and desires, the community health centers have made continuous efforts to put nutritional activities into practice in the community. However, there are constraints, such as relative staff shortages, lack of funds, and information which hampers the nutritional activities.

• Journal of the Korean Dietetic Association
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• v.11 no.1
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• pp.114-124
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• 2005

The nutritional status of pre-school children is important for both physical growth and functional development. This study investigated the anthropometric, nutrient intakes and dietary quality of the pre-school children living in Asan for planning nutritional education program. The dietary intakes were measured by 2-day 24 hr recall recorded by children's mothers and the anthropometric data were collected by measuring children's weights, heights, and percent body fat at Asan Public Health Center. The children were 161 boys and 129 girls aged 5 and 6 years. When children's nutritional status was determined by their anthropometric status (underweight, normal weight and overweight) determined by z-score (normal range between -1.00 and 1.00), only 1.4% of the children belonged to underweight group while 55.7% and 42.9% belonged to normal and overweight groups, respectively. Significantly higher number of children belonged to overweight group for 5 year-olds. No differences were observed in nutrient intakes, nutrient adequacy ratio (NAR) and index of nutritional quality (INQ) values among nutritional status groups. Intakes of the most nutrients were adequate, but NAR and INQ of calcium and zinc were low. When nutrient intakes, NAR and INQ were compared by sex or age, all nutrient intakes were appropriate except calcium and zinc intakes of 6 year-olds. Nutrient intakes of 5 years were higher than those of 6 years. The mean of nutrient adequacy ratio (MAR) was 0.85 and mean INQ was 1.51 for all participants. The nutritional management program for pre-school children in Asan would be directed differently by age groups and education material should contain contents educating to consume moderate amount of food for 5 year-olds and to increase intake of foods with mineral nutrients such as calcium and zinc for 6 year-olds.