• Horticultural Science & Technology
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• v.32 no.5
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• pp.730-738
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• 2014

The objective of this study was to evaluate glucosinolate (GSL) profiles and variation of total and individual GSLs concentrations within seedling (0-14 days) and growth stages (0-15 weeks) of Korean Chinese cabbage (Brassica rapa L. ssp. pekinensis). Ten GSLs (progoitrin, glucoraphanin, glucoalyssin, gluconapin, glucobrassicanapin, 4-hydroxyglucobrassin, glucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin and gluconasturtiin) were identified from Korean Chinese cabbage. In general, total GSL content significantly decreased during seedling (from 92.89 to $35.26{\mu}mol{\cdot}g^{-1}$ DW) and g rowth stages ( from 74.11 to $1.97{\mu}mol{\cdot}g^{-1}$ DW). Gluconapin was the highest in seeds and in the germination period ($73.1{\mu}mol{\cdot}g^{-1}$ DW) and declined gradually from 73 to 15% during seedling stages. The level of the major aliphatic GSLs, gluconapin and progoitrin, tended to decrease sharply, whereas levels of indolic GSLs (4-methoxyglucobrassicin, glucobrassicin) and aromatic GSLs (gluconasturtiin) were found to increase generally at the beginning of growth stages.

• The Korean Journal of Food And Nutrition
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• v.8 no.2
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• pp.105-109
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• 1995

This study was devised to observe the inhibitory effects of 3 kinds of petroleum ether extracts (percolation by petroleum ether) from Korean red ginseng, Chinese red ginseng and American white ginseng on a lipolytic action of Toxohormone-L which has been known as lipolytic and anorexigenic factors. Toxohormone-L was obtained by partial purification of the ascites fluid from mice which had been Inoculated with sarcoma-180. The yields of petroleum ether extract from Korean red ginseng, Chinese red ginseng and American white ginseng were 0.64, 0.47 and 0.58 and respectively, indicating that the yield of Korean red ginseng was the highest. In vitro, at the concentration of 2 mg /ml, the inhibition rate of lipolysis by the petroleum ether extract of Korean red ginseng, Chinese red ginseng and American white ginseng were 55.1, 50.0 and 44.9% respectively, and the total inhibitory activity per gram of ginseng material were 18, 12 and 13 unit respectively, indicating that the Korean red ginseng was the most effective in the inhibition of the lipolysis.

• Fisheries and Aquatic Sciences
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• v.15 no.3
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• pp.191-195
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• 2012

We report here the use of high-speed countercurrent chromatography (HSCCC) in the preparative isolation and purification of the bioactive component, fucosterol, from Pelvetia siliquosa. A crude extract was obtained by ultrasonic extraction of powdered P. siliquosa using methylene chloride and was then subjected to separation and purification by HSCCC, coupled with evaporative light-scattering detection. Preparative HSCCC was performed successfully using a two-phase solvent system, n-heptane:methanol (3:2, v/v), to obtain 10.96 mg fucosterol with 96.8% purity from 50 mg of crude extract; the recovery rate was approximately 90.5%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.1
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• pp.102-108
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• 1998

This study was performed to establish manufacturing of garlic vinegar which has medical action component of garlic and functionality of vinegar. Optimum alcohol concentration, and garlic juice concentration for the mpnufacturing of garlic vinegar were 5%, 10%, respectively, and the optimum conditions were obtained when the fermentation was carried out at agitation of agitation of 120rpm and temperature of 3$0^{\circ}C$. The acetic acid content of optimum condition was 4.87% and thiosulfinate content was 37.5mg after 20days fermentation. The major organic acid compositions in garlic vinegar were oxalic acid, citric acid, malic acid and acetic acid, and free amino acid compositions were aspartic acid, threonine, serine, glutamic acid, alanine etc.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.4
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• pp.376-380
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• 1991

The contents of total lipids from Korean marine benthic algae Miyeok(Undaria pinnatifida) and Pare(Enteromorpha compressa)were 1.8% and 0.7% on the dry basis, respectively. They were fractionated by silicic acid column chromatography and identified by gae liquid chromatography. The ratios of neutral lipids, glycolipids and phospholipids in total lipids were 3.5 : 74.1 : 22.4 in Miyeok and 33.1 : 48.4 : 18.5 in Pare. Total fatty acids in Miyeok and Pare were composed of 28.5 and 33.2% of saturates, 9.8 and 10.8% of monoenes, 61.7 and 56.0% of polyenes, respectively and the polyunsaturated acid was the most predominent component. $\omega$-3 polyunsaturated fatty acids were C18:4(15.9 and 17.1%) and C20:5(10.6 and 6.0%).

• Preventive Nutrition and Food Science
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• v.13 no.2
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• pp.122-127
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• 2008

The aroma constituents of Chrysanthemum indicum L. were separated by the hydro distillation extraction method using a Clevenger-type apparatus, and were analyzed by gas chromatography-mass spectrometry (GC/MS). The yield of Chrysanthemum indicum L. flower oil was 2.0% (w/w) and the color was light golden yellow. Sixty-three volatile flavor components, which make up 89.28% of the total aroma composition of the flower oil, were tentatively characterized. This essential oil contained 35 hydrocarbons (48.75%), 12 alcohols (19.92%), 6 ketones (15.31%), 3 esters (4.61%), 5 aldehydes (0.43%), 1 oxide (0.22%), and 1 miscellaneous component (0.04%). ${\alpha}$-Pinene (14.63%), 1,8-cineol (10.71%) and chrysanthenone (10.01%) were the predominant volatile components in Chrysanthemum indicum L., an aromatic medicinal herbaceous plant.

• Preventive Nutrition and Food Science
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• v.13 no.2
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• pp.128-133
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• 2008

The volatile flavor components were isolated from the roots of Saussurea lappa C.B. Clarke produced in Korea and China by the hydro distillation, and were analyzed by gas chromatography-mass spectrometry (GC/MS). 63 aroma compounds representing 87.47% of the total peak area were tentatively identified, including 13 alcohols (22.56%), 26 hydrocarbons (21.78%), 4 aldehydes (21.24%), 11 ketones (18.04%), 1 oxide (0.52%), 3 esters (0.16%), 1 carboxylic acid (0.02%) and 4 miscellaneous components (3.15%). 46 volatile flavor components of imported S. lappa C.B. Clarke constituted 65.69% of the total volatile composition were tentatively characterized, consisting of 1 aldehyde (23.32%), 24 hydrocarbons (16.69%), 10 ketones (15.84%), 7 alcohols (8.92%), 1 oxide (0.83%), 2 esters (0.07%) and 1 acid (0.02%). The predominant components of both essential oils were (7Z,10Z,13Z)-7,10,13-hexadecatrienal and dehydrocostuslactone.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.2
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• pp.292-298
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• 1999

Myofibril and actomyosin were prepared from red muscle and white muscle of fresh water fish and sea water fish, and their biochemical characteristics and SDS PAGE patterns of myofibril were compared. SDS PAGE analysis showed that electrophoretic patterns of myofibril were similar be tween white muscle and red muscle, while difference of 30kDa component of myofibril was detected between fresh water fish and sea water fish. When myofibril were treated with trypsin, difference in hydrolysis of heavy chain was observed between white muscle and red muscle. In activities of Ca ATPase, Mg ATPase, EDTA ATPase and ATPase activity pH curve, myofibrillar protein from fresh water fish showed higher specific activity than those from sea water fish.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.2
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• pp.326-332
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• 1999

In order to develope useful components and to apply in food additives, various components in the leaves of baechohyang were determined according to picking date. Moisture and ash were reached to 74~82%, 1.5~3.4%, respectively, and major sugars were fructose, glucose, sucrose, and maltose. Glutamic, aspartic acid, and leucine constituted the main component amino acids and their contents were relatively higher in the samples of summer and young leaves. Among free amino acids, histidine showed the highest components and methionine were the lowest. Potassium, calcium, and sodium were main mineral components. The content of ascorbic acid were 6.6~9.2mg% and seasonal variations were negligible. Muture leaves contained more polyphenols as much as 108~181mg% and the amount of bound fractions were much more. Periodic evaluation of phenolic compounds were made and major components of bound fractions were caffeic, syringic, and ferulic acid.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.1
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• pp.153-160
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• 2000

We investigated the cytotoxic effects of roots and seeds Daucus carota L. on HepG2, HeLa, MCF7, SW626, C6 and NB41A3 cell lines by the MTT assay. Among extracts, the ethylacetate partition layer (DCMEA) of root of Daucus carota L. showed the strongest cytotoxic effects on HepG2, HeLa, C6 and NB41A3 cell lines. On the other hand, methanol(DCM), n-ethylacetate(DCMEA) and n-butanol(DCMB) extract of the seeds of Daucus carota L. also showed significant cytotoxic activities for all six cell lines. $\beta$-carotene, a well-known main component of Daucus carota L. was also tested for its cytotoxic effect. However, in all six cell lines, $\beta$-carotene falied to show significant cytotoxicity. Therefore, the anticancer effect of DCMEA of root fo Daucus carota L. and DCM, DCMH, DCMEA and DCMB extracts of seeds may be caused by components other than $\beta$-carotene. Futher studies are under way to isolate the compounds responsible for the significant cytotoxic activity.