Browse > Article
http://dx.doi.org/10.5657/FAS.2012.0191

Isolation of Fucosterol from Pelvetia siliquosa by High-speed Countercurrent Chromatography  

Hwang, Seung Hwan (Department of Food Science and Nutrition, Hallym University)
Jang, Jai Man (Institute of Natural Medicine, Hallym University)
Lim, Soon Sung (Department of Food Science and Nutrition, Hallym University)
Publication Information
Fisheries and Aquatic Sciences / v.15, no.3, 2012 , pp. 191-195 More about this Journal
Abstract
We report here the use of high-speed countercurrent chromatography (HSCCC) in the preparative isolation and purification of the bioactive component, fucosterol, from Pelvetia siliquosa. A crude extract was obtained by ultrasonic extraction of powdered P. siliquosa using methylene chloride and was then subjected to separation and purification by HSCCC, coupled with evaporative light-scattering detection. Preparative HSCCC was performed successfully using a two-phase solvent system, n-heptane:methanol (3:2, v/v), to obtain 10.96 mg fucosterol with 96.8% purity from 50 mg of crude extract; the recovery rate was approximately 90.5%.
Keywords
Pelvetia siliquosa; Fucosterol; High-speed countercurrent chromatography; Evaporative light-scattering detection;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Friesen JB and Pauli GF. 2007. Rational development of solvent system families in counter-current chromatography. J Chromatogr A 1151, 51-59.   DOI   ScienceOn
2 Hagiwara H, Wakita K, Inada Y and Hirose S. 1986. Fucosterol decreases angiotensin converting enzyme levels with reduction of glucocorticoid receptors in endothelial cells. Biochem Biophys Res Commun 139, 348-352.   DOI   ScienceOn
3 Ikeda I, Tanaka K, Sugano M, Vahouny GV and Gallo LL. 1988. Inhibition of cholesterol absorption in rats by plant sterols. J Lipid Res 29, 1573-1582.
4 Ito Y. 2005. Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography. J Chromatogr A 1065, 145-168.   DOI   ScienceOn
5 Lee S, Lee YS, Jung SH, Kang SS and Shin KH. 2003. Anti-oxidant activities of fucosterol from the marine algae Pelvetia siliquosa. Arch Pharm Res 26, 719-722.   DOI   ScienceOn
6 Lee S, Lee YS, Jung SH, Lee HS, Kang SS and Shin KH. 2004a. Determination of fucosterol in the marine algae Pelvetia siliquosa by gas chromatography. Nat Prod Sci 10, 29-31.
7 Lee YS, Jung SH, Lee S, Choi YJ and Shin KH. 2002. Hepatoprotective and anti-diabetic effects of Pelvetia siliquosa, a marine algae, in rats. J Appl Pharmacol 10, 165-169.
8 Lee YS, Shin KH, Kim BK and Lee S. 2004b. Anti-diabetic activities of fucosterol from Pelvetia siliquosa. Arch Pharm Res 27, 1120- 1122.   DOI   ScienceOn
9 Marston A and Hostettmann K. 1994. Counter-current chromatography as a preparative tool: applications and perspectives. J Chromatogr A 658, 315-341.   DOI
10 Marston A and Hostettmann K. 2006. Developments in the application of counter-current chromatography to plant analysis. J Chromatogr A 1112, 181-194.   DOI   ScienceOn
11 Oh YS, Lee IK and Boo SM. 1990. An annotated account of Korean economic seaweeds for food, medical and industrial uses. Kor J Phycol 5, 57-71.   과학기술학회마을
12 Tsao R and Deng Z. 2004. Separation procedures for naturally occurring antioxidant phytochemicals. J Chromatogr B Analyt Technol Biomed Life Sci 812, 85-99.   DOI   ScienceOn
13 Wang X, Liu J, Zhang T and Ito Y. 2007. Rapid and simple method for quality control of raw materials of herbs by HSCCC. J Liq Chromatogr Relat Technol 30, 2585-2592.   DOI   ScienceOn
14 Yoon JT. 1995. Developmental morphology on the regeneration of Pelvetia siliquosa Tseng et Chang, Phaeophyta in Korea. Ph.D. Dissertation, University of Chuonbuk, Jeonju, Korea.