• Food Science of Animal Resources
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• v.37 no.6
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• pp.940-947
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• 2017

Goat milk has a protein composition similar to that of breast milk and contains abundant nutrients, but its use in functional foods is rather limited in comparison to milk from other sources. The aim of this study was to prepare a goat A2 ${\beta}$-casein fraction with improved digestibility and hypoallergenic properties. We investigated the optimal conditions for the separation of A2 ${\beta}$-casein fraction from goat milk by pH adjustment to pH 4.4 and treating the casein suspension with calcium chloride (0.05 M for 1 h at $25^{\circ}C$). Selective reduction of ${\beta}$- lactoglobulin and ${\alpha}_s$-casein was confirmed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography. The hypoallergenic property of A2 ${\beta}$-casein fraction was examined by measuring the release of histamine and tumor necrosis factor alpha from HMC-1 human mast cells exposed to different proteins, including A2 ${\beta}$-casein fraction. There was no significant difference in levels of both indicators between A2 ${\beta}$-casein treatment and the control (no protein treatment). The A2 ${\beta}$-casein fraction is abundant in essential amino acids, especially, branched-chain amino acids (leucine, valine, and isoleucine). The physicochemical properties of A2 ${\beta}$-casein fraction, including protein solubility and viscosity, are similar to those of bovine whole casein which is widely used as a protein source in various foods. Therefore, the goat A2 ${\beta}$-casein fraction may be useful as a food material with good digestibility and hypoallergenic properties for infants, the elderly, and people with metabolic disorders.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.5
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• pp.682-686
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• 2002

Ten growing male buffalo calves (aged 6-8 months, average body weight $88.2{\pm}0.57$ kg were divided randomly into two groups. Animals in group I were fed on concentrate mixture A (CP 20.2%, TDN 77.4%) and urea- ammoniated wheat straw (UAWS) while the animals in group II were fed on concentrate mixture B (CP 17.9%, TDN 77.6%) and HCl plus urea treated wheat straw (HCl UAWS) to meet their nutrient requirement for 500 g gain/d as per Kearl (1982). This feeding practice lasted for 120 days, during which fortnightly body weight were recorded to assess their growth rate. A metabolism trial was conducted after 90 days of experimental feeding to compare the digestibility of nutrients, their balance, plane of nutrition and relative cost of feeding in two groups of animals. Results revealed a significant increase in the CP content of ammoniated wheat straw due to addition of HCl viz 12.1% from 7.5%. There was a decrease in the intake of DM (p<0.05), OM (p<0.05), EE (p<0.05), NDF (p<0.01), ADF (p<0.01), cellulose (p<0.01) and hemicellulose (p<0.01) in group II as compared to group I. The digestibility (%) of DM, OM and CP was significantly (p<0.01) more in group II, whereas the digestibility (%) was significantly more for NDF (p<0.05) and hemicellulose (p<0.01) in group I than group II. There was no significant difference in the N, Ca and P balance in two groups. Intake of total DM (g/d) or (g/kg $W^{0.75}$) was significantly (p<0.01) more in group I as compared to group II, whereas the intake of DCP and TDN (g/d or g/kg $W^{0.75}$) was alike in two groups. The total body weight gain (kg), average daily gain (g/d) and feed conversion efficiency were significantly (p<0.01) more in UAWS fed group as compared to HCl UAWS fed group. Feeding cost (Rs./kg. weight gain) was significantly (p<0.05) more in group II as compared to group I. It is concluded that HCl UAWS is not suitable for the feeding of growing buffalo calves as it reduced the growth rate in comparison to UAWS fed buffalo calves.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.6
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• pp.807-817
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• 2008

Steers with ad libitum access to rice straw were assigned to four diets to evaluate the effects of maize or soybean hull supplementation on intake, in vivo digestibility, ruminal pH, VFA, ammonia-nitrogen ($NH_3-N$) and in situ ruminal disappearance of feed nutrients by cattle consuming rice straw. Supplement treatments were: no supplement (RS); soybean meal at 0.127% BW (SBM); cracked maize at 0.415% BW plus 0.044% BW soybean meal (MAIZE); or soybean hulls at 0.415% BW plus 0.044% BW soybean meal (HULLS). The MAIZE and HULLS diets were formulated to provide approximately 4 MJ of $NE_m$ per kg of diet. Rice straw DMI was not affected (p = 0.34) by supplement. Apparent dry matter (DM) digestibility was greater (p<0.001) for MAIZE and HULLS (56.6 and 60.0%, respectively) than for steers consuming SBM or RS (51.8 and 44.4%, respectively). Apparent NDF digestibility was greater (p<0.0004) for HULLS than MAIZE (61.7 vs. 58.0%, respectively) and apparent ADF digestibility was greater (p<0.0008) for HULLS than MAIZE (61.1 vs. 49.2%, respectively). There was no difference in apparent hemicellulose digestibility (p = 0.43). Analysis of ruminal fluid collected 0, 2, 4, 6, and 8 h post-feeding revealed ammonia-nitrogen was greatest (p<0.05) for steers on SBM and HULLS diets at 2 h (24.08 and 22.57 mg/dl, respectively) and total volatile fatty acids was greatest (p<0.05) for HULLS at 4 h (230 mM/L). In situ disappearance, measured at 0, 2, 4, 6, 8, 16 and 24 h, indicated that SBM, MAIZE and HULLS tended to enhance the digestibility of DM and fiber components of rice straw. In situ disappearance of rice straw DM was greatest for SBM and/or HULLS from 4 to 24 h (p = 0.03). Rice straw NDF and ADF disappearance was enhanced by supplementation from 16 to 24 h (p<0.02). Rice straw DM, NDF and ADF disappearances at 24 h were similar for MAIZE and HULLS treatments. When feeding cattle rice straw diets, energy and protein-based supplements are essential. This study showed that fiber-based supplements are just as, if not more, effective as starch-based supplements in rice straw utilization. This study shows that soybean hulls, in spite of their high fiber content, are as efficient as maize for supplementing rice straw primarily because fiber in soybean hulls is highly digestible as shown by in vivo digestibility and in situ disappearance.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.1
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• pp.70-78
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• 1991

Both long period of summer drought and unusual high water temperature in 1988 caused the harmfull effect to the aquacultural organisms in south coast of Korea. One of those was the mass mortality of arkshell seedlings in Jinhae Bay. The purpose of this study was to verify the effects of environmental factors on arkshell seedlings by investigating not only water quality and food organisms in culturing grounds, but also meteorological conditions. Water temperature in November was higher at $2.0^{\circ}C$ than mean water temperature and the concentration of total inorganic nitrogen and N/P ratio were $0.38{\mu}g-at/l,$ 0.98, respectively which indicated the exhaustion of nutrients, especially nitrogen in the study area. In analysis of phytoplankton population structure, species diversity index(H) , equitability-index(e), species richness $index(d_1)$ were low, but dominance $index({\delta}_2)$ was high at the value of 98.4 and dominant species was Nitzschia pungens. In conclusion, it seems that mass .mortality of arkshell seedlings in November was resulted from the metabolism disorder with poor food organism under the unstable marine environment.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.1
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• pp.53-60
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• 2020

Objective: The current study was carried out to examine the response of different levels of fat and protein in calf starter on nutrient utilisation, nitrogen metabolism, weight gain, blood parameters, and immunity level in pre-ruminant calves. Methods: Twenty four calves (5 days old) were divided into six groups in a 2×3 factorial design, with two levels of fat (10% and 14%) and three levels of protein (18%, 21%, and 24%). The calves were kept in individual pens for 120 days and fed with whole milk (1/10th of body weight) and calf starter ad-libitum. Daily dry matter intake was recorded; whereas body weight was taken on fortnightly basis to calculate average daily gain. During the growth trial blood samples were collected at 30 days interval to estimate blood glucose, albumin, total protein, total leucocyte count, total immunoglobulins and immunoglobulin G levels. A metabolic trial of seven days was carried out to find out the digestibility of different nutrients. Results: The dry matter intake was reduced (p<0.05) with higher fat and protein levels whereas feed conversion efficiency was improved (p<0.05) with higher protein level. Different levels of fat and protein in calf ration did not affect average daily gain in calves. The dry matter, organic matter, and crude protein digestibility were significantly (p<0.01) higher with increased level of protein. The nitrogen retention was also significantly higher (p<0.05) at 24% protein level, similarly the total immunoglobulin was significantly (p<0.05) high in higher protein fed groups, showed better immunity. Conclusion: The present finding suggested that 10% fat and 18% protein level of calf starter could be used in Sahiwal calves for optimum performance in terms of weight gain and immunity.

• Korean Journal of Poultry Science
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• v.11 no.2
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• pp.93-98
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• 1984

Four different silages were prepared out of citrus peel with or without supplementation with wheat bran and/or urea. Proximate nutrients and volatile fatty acids were analysed on the silages, both fresh and dried. All silages were dried and ground, and were compared with dried citrus peel (not ensiled) and wheat bran in a folding trial. A total of 360 male chicks of Maniker broiler strain was divided into and assigned to 6 treatments with 5 replications. Each experimental diet included each of 6 test material at the level of 6% and the feeding trial lasted 8 weeks, after which a metabolism trial was conducted. Results obtained were summarized as follows. 1. Ensiling increased protein content of the citrus peel on the dry matter basis and supplementing the silage material with wheat bran and/or urea further increased protein contents of the silages. 2. Adding wheat bran and/or urea to silage material also increased volatile fatty acid contents of the silages. After drying organic acid contents of the silages were reduced and there were no differences in organic acid contents of the dried citrus peel silages, differently treated, 3, Broilers fed dried citrus peel and those fed dried citrus peel silages gained numerically less weight than those fed wheat bran. But there were no significant differences in body weight gain, feed intake and feed efficiency among different treatments 4. There were no significant differences in nutrient utilizability, nitrogen retention and metabnlizable energy contents among treatments. It could be concluded that ensiling is an effective way of storing citrus peel and dried citrus peel silage could be used in broiler diets up to 6% without adverse effects.

• Journal of Ginseng Research
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• v.3 no.1
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• pp.1-34
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• 1979

Our nation is confronted with the situation that the rice, a principal food, short of some essential amino acids, leads to imbalanced meals insufficient in the nutrient of Protein, to bring many difficulties in the elevation of nutritional state in our nation. While. our country has been produced much amounts of Panax Ginseng roots which has a stimulating effects on the metabolism of protein, lipid and nucleic acids in the body. And the leaf and trunk of Panax Ginseng were also produced a considerable amounts as the by-products. Author believe that these by-products (leaf and trunk) of Panax Ginseng might have some components possessing simillar activity with Panax Ginseng root although the quantity and qualify of the functional components may more or less be different. Therefore, this study was demised to observe the supplemental effect of the Panax Ginseng-by-Products on the dietary protein efficiency and nutritional state of rats. The feeds used for this experiment were rice containing 30% barely, fish four, and the leaf, trunk and small root of the Panax Ginseng, and the contents of the general nutrients including protein, lipid and carbohydrate etc. in each feed were analyzed for the combination of each feed. And, being based on analytical values of Protein in food. fish Pour as Protein source was added were rice containing 30% barely to be include 8.6 to 8.7%, 12%, 15% and 18% of protein. Then 2% of the leaf, trunk or small reef of Panax Ginseng was supplemented into each of above protein diet group, ton 16 kinds of diets were Prepared. The male albino rats from a Pure strain, weighing 70g to 80g. were used for experimental animals. They were maintained with coresponding fist for f and 8 weeks, and the growth rate, consumption of diets and protein, efficiency of feed and Protein in animals were determined. The lipids, proteins and cholesterols in serum and liver were also determined quantitatively after they were sacrificed in coresponding term. The results obtained are summarized as follows: 1. Body weigh of diet group containing 8.6 to 8.7%,12%, and 15% of protein are increased remarkably by supplement of 2% of the leaf or small root of Panax Ginseng in comparison with each of controls. But this tendency could not observed in diet group containing 18eA Proteins. 2. Feed efficiency showed same tendency in comparison with changes of gained body weight. Specially, in each of diets containing 8.7%, 12%, 15% and 18% of Proteins, supplement of the leaf of Panax Ginseng showed the better feed efficiency than supplement of the trunk or small root. 3. In feeding group for 8 weeks, protein efficiency showed worst efficiency in diet containing 18% proteins and showed the best efficiency was the diet group containing 12% Proteins. And the efficiency was improved according to supplement of the leaf of Panax Ginseng. 4. Nitrogen contents in serum and liver did not show large differences each other in all diet groups. But contexts of total cholesterol and 1ipid were decreased markedly in diet groups containing 12%, 15% and 18% of proteins in comparison with diet group containing 8.6% to 8.8% of proteins.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.5
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• pp.290-303
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• 2003

Modern agriculture has been heavily dependent on chemical fertilizers to meet the food demands of ever increasing population. Progressive depletion of major plant nutrients in soil due to intensive cultivation practices has also necessitated the use of higher dose of chemical fertilizers particularly in soils where the organic matter content is very low. Indiscriminate use of chemical fertilizers and pressure on agriculturists to enhance per area crop yields has led to fast depletion of fossil fuel resources with concomitant increase in the prices of chemical fertilizers and also led to environmental pollution. Hence, the current trend throughout the world is to explore the possibility of using alternate nutrient sources or increasing the efficiency of chemical fertilizers by supplementing them with organic fertilizers and bioinoculants comprising largely microbes like, bacteria, fungi, algae etc to enhance nitrogen and phosphates in the soil thus creating a sustainable agricultural environment. Among the different microbial inoculants or biofertilizers, Azospirillum could be a potential candidate due to its non specific host root colonization. It had the capability to fix $N_2$ in wide pH regimes and even in presence of combined nitrogen. Azospirillum inoculation can increase the crop yield to 10-25% and substitute 25% of recommended doses of nitrogenous fertilizers. Apart from nitrogen fixation, Azospirillum is also involved in the root improvement, the activity which was attributed to an increase in the rate of water and mineral uptake by roots. The ability of Azospirillum to produce phytohormones was reported to enhance the root respiration rate, metabolism and root proliferation. They have also been reported to produce polyhydroxybutyrate, that can be used as a biodegradable thermosplastic. A lot of studies have addressed improvements in enhancing its efficiency to fix nitrogen fixation and hormone production.

• Journal of Animal Science and Technology
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• v.65 no.6
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• pp.1226-1241
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• 2023

Selenium (Se) is an essential trace mineral that plays an important role in physiological processes by regulating the antioxidant defense system and enhancing immunity. Chromium is an essential mineral involved in carbohydrate and lipid metabolism and also plays a role in maintaining normal insulin function. Based on these advantages, we hypothesized that the addition of selenomethionine (SeMet) and organic chromium (OC) to broiler diets would increase Se deposition, antioxidant capacity and immune response in meat. Therefore, this study analyzed the effects of OC and SeMet on growh performance, nutrients digestibility, blood profiles, intestinal morphology, meat quality characteristics, and taxonomic analysis of broilers. A total of 168 one-day-old broiler chicken (Arbor Acres) were randomly allotted to 3 groups based on the initial body weight of 37.33 ± 0.24 g with 7 replicate per 8 birds (mixed sex). The experiments period was 28 days. Dietary treatments were folloewd: Basal diets based on corn-soybean meal (CON), basal diet supplemented with 0.2 ppm OC and 0.2 ppm SeMet (CS4), and basal diet supplemented with 0.4 ppm OC and 0.4 ppm SeMet (CS8). Supplementation of OC and SeMet did not affect on growth performance, nutrient digestibility. However, CS8 supplementation increased in duodenum villus height and villus height : crypt depth, and increased in breast meat Se deposition. In addition, CS8 group showed higher uric acid and total antioxidant status than CON group. Taxonomic analysis at phylum level revealed that Proteobacteria and Firmicutes of CS4 and CS8 were lower than CON group. In genus level, the relative abundance of fecal Lactobacillus and Enterococcus of CS4 and CS8 groups were higher than CON group. In short, 0.4 ppm OC and 0.4 ppm SeMet supplementation to broiler diet supporitng positive gut microbiome change, also enhancing antioxidant capacity, and Se deposition in breast meat.

• Journal of Animal Science and Technology
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• v.49 no.5
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• pp.667-676
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• 2007

This study was conducted to determine effects of cellulolytic microbes inoculation to sawdust-based spent mushroom substrate(SMS) during deepstacking on fermentation parameters, total microbial counts and cellulolytic enzyme activity and to on SMS nutrients utilization by sheep. For sheep metabolism trials, six sheep(ram, average 54.8kg) were fed a Control diet(70% concentrates, 15% rice straw and 15% SMS with no microbial treatment on a dry basis) and a Treatment diet(the same diet including SMS with a microbial treatment) for 2 trials. Spent mushroom substrates with or without a microbial(4 strains including 1 strain of Enterobacter ludwigii, 1 strain of Bacillus cereus and 2 strains of Bacillus subtillis) treatment (1% of SMS on wet basis) were deepstacked for 7 days. The internal temperatures in 1.2 M/T of SMS deepstacks reached to 50±5℃ within 7 days of storage. Total microbial counts remarkably decreased (P<0.05) with a deepstacking process and were not affected(P>0.05) by the microbial treatment. For fibrolytic enzyme activity, CMCase and xylanase activities were decreased(P<0.05) by a deepstacking process. After deepstacking, the microbial treatment showed about 2.5-times higher(P<0.05) for CMCase activity and about 4-times higher(P<0.05) for xylanase activity than those of the Control. Activities of ligninolytic enzymes such as laccase and MnP were not affected by the microbial treatment. The sheep fed the microbially treated SMS diet had a tendency of greater total tract digestibilities of ash(P=0.051), NFE (P=0.071), hemicellulose(P=0.087) and NDF(P=0.096) than those fed the untreated SMS diet. Nitrogen balance of sheep was not affected(P>0.05) by feeding of microbially treated SMS. Accordingly, these results indicate that cellulolytic microbes inoculation during deepstacking of SMS may improve the bio- utilization of SMS by sheep.