• Korean Journal of Microbiology
• /
• v.28 no.4
• /
• pp.290-296
• /
• 1990

Putrescine, spermidine, and spermine were found to persent in Methylobacterium extorquens AM1 growing on methanol, succinate, glucose, or nutrient broth as an energy source. Spermidine was found to be a major polyamine in cells growing on methanol or succinate, while putrescine to be the one in nutrient broth-grown cells. The overall content of polyamines in cells growing on glucose was less than that in cells growing on other substrates. Spermine was the most abundant polyamine in glucose-grown cells. Accumulation of polyamines in M. extorquens AM1 was maximal at the mid-exponential or early stationary phase during growth on each substrate. The effect of polyamines added into the medium on the polyamine composition in M. extorquens AM1 was variable. Each polyamine added into the nutrient broth medium was found to increase the amount of the respective polyamine in the cell. Exogeneously added polyamines had no effect on the growth of M. extorquens AM1.

• Asian-Australasian Journal of Animal Sciences
• /
• v.19 no.1
• /
• pp.31-34
• /
• 2006

The experiment was conducted to determine the effects of cutting interval and cutting height on the yield and nutrient composition of hedge lucerne (Desmanthus virgatus) when grown on a sandy soil in the Northeast of Thailand. The cutting intervals compared were 30, 40 and 50 days between harvests and the cutting heights 30, 40 and 50 cm above ground level. The experiment was a $3{\times}3$ factorial layout in a randomized complete block design with 4 replications-giving a total of 36 plots each $3{\times}3m^2$. Harvested plant material was weighed, dried and the ground subsamples taken for analyses of crude protein (CP), crude fiber (CF), ash, ether extract (EE) and nitrogen-free extract (NFE). At the last harvest the hedge lucerne samples were separated to determine leaf to stem ratios and then analyzed for nutrient composition in the leaf and stem. Results showed that increasing the cutting interval (i.e. advancing age of maturity) increased dry matter and nutrient yields significantly. In terms of nutrient content, it also increased the crude fiber, ash, ether extract and nitrogen free extract percent in the plant. However, crude protein percent was markedly decreased as the cutting interval increased. Increasing cutting height had no effect on dry matter yield and yields of nutrients, but in terms of nutrient content, it increased crude protein and ash content, but decreased crude fiber content. The percent EE and NFE in the plant was unaffected by cutting height. From the results presented it is clear that cutting a stand of hedge lucerne every 40 to 50 days will achieve greater dry matter and nutrient yields than cutting more frequently, at 30 days. The cutting height at harvest, whether 30, 40 or 50 cm above ground level had no effect on dry matter or nutrient yields of hedge Lucerne. Hedge lucerne therefore offers the Thai poultry farmer a useful alternative protein supplement for poultry diets rather than relying on the more expensive soybean meal. As it can be readily and successfully grown on a range of soil types and climates throughout Thailand, hedge lucerne also offers the Thai farmer a valuable additional source of income.

• Korean journal of food and cookery science
• /
• v.20 no.1
• /
• pp.100-111
• /
• 2004

The purpose of this study was to investigate the status of current nutrition labeling on the packaging of the processed foods that provide consumers with a reliable and consistent source of information, which has been considered as a useful aid for food selection and a potent educational tool for nutrition in daily life. The 2,160 processed foods purchased at the supermarket on September, 2002, were divided by food category issued from the 2002 food codes and assessed in the terms of the nutrition composition labeling and nutrition claims. Nutrition composition labeling was found on 356 of the 2160 processed foods items. Milk and dairy products had 49.7％ of nutrition composition labeling, which was the largest number among the food category. Tables were most frequently used as the type of nutrition composition labeling (79.8％). Nutrition composition including many different ways of expression, such as a table of nutrition composition, indication of nutrition composition, analysis table of nutrition composition and comparative table of nutrition composition, made frequent use of nutrition composition labeling titles (78.7％). The various unit of measures were use in the nutrition labeling of the processed foods, per l00g or 100$m\ell$ was the highest (44.6％) under the currently practiced nutrition labeling. The correct labeling standard with nutrient content and ％ RDA except energy, was used on 47.8％ of labels, and those with only liability indication nutrient and liability indication nutrients plus discretion indication nutrients were 25.3 and 22.5％ respectively. The processed foods with nutrition claims were 8.0％ (172 items). Nutrition claims were divided in two ways: nutrient content claims and nutrient comparative claims. The most frequently used claims were contained in the former (44.4％) and more or plus in the latter case (16.3％). Ca was the most popular item as a nutrition claim nutrient (50.6％).

• Journal of Microbiology and Biotechnology
• /
• v.3 no.2
• /
• pp.115-122
• /
• 1993

Poly(3-hydroxybutyrate)(P(3HB)) accumulation under nutrient-rich condition with various amounts of $(NH_4)_2 SO_4$ was systematically investigated. The results of the electron-microscopy and the solvent extraction showed that the P(3HB) accumulation is unavoidable even under nutrient-rich condition. This indicates that in a two-step culture of Alcaligenes eutrophus H16, the researches should be careful in interpreting the data of polyhydroxyalkanoates(PHAs) accumulation in terms of the carbon-source fed in the second step because the two-step culture product contains the P(3HB) produced under nutrient-rich condition. The polyester production capability in a two-step batch culture of A. eutrophus H16(ATCC 17699) was also investigated using various saccharides and their derivatives such as glucose, fructose, gluconic acid, glucaric acid, sorbitol, lactose, galactose, and mannose. The polyesters synthesized were characterized by 500 MHz$^{1}H-NMR$ spectroscopy, intrinsic viscosity$[\eta]$ measurement in chloroform and differential scanning calorimetry(DSC). 500 MHz $^{1}H-NMR$ analysis showed that all polyesters synthesized generally contained 1~2 mol% of 3HV. Another finding is that the glucose utilization can be increased by changing the autoclaving procedure of the substrate to enhance the P(3HB) production yield up to 46 wt% of P(3HB) in dry cells.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.1
• /
• pp.59-65
• /
• 2008

Six total mixed rations (TMR) containing 0, 4, 6, 8, 10, 12% tannin (TMR I-VI), using Accacia nilotica pods as a source of tannin, were used to study the effect of Acacia tannin on in vitro nutrient digestibility and gas production in goats. This study also investigated the degraded products of Acacia nilotica tannin in goat rumen liquor. Degraded products of tannins were identified using high performance liquid chromatography (HPLC) at different hours of incubation. In vitro digestibility of dry matter (IVDMD) and organic matter (IVOMD) were similar in TMR II, and I, but declined (p<0.05) thereafter to a stable pattern until the concentration of tannin was raised to 10%. In vitro crude protein digestibility (IVCPD) decreased (p<0.05) with increased levels of tannins in the total mixed rations. Crude protein digestibility was much more affected than digestibility of dry matter and organic matter. In vitro gas production (IVGP) was also reduced (p<0.05) with increased levels of tannins in the TMR during the first 24 h of incubation and tended to increase (p>0.05) during 24-48 h of incubation. Gallic acid, phloroglucinol, resorcinol and catechin were identified at different hours of incubation. Phloroglucinol and catechin were the major end products of tannin degradation while gallate and resorcinol were produced in traces. It is inferred that in vitro nutrient digestibility was reduced by metabolites of Acacia nilotica tannins and ruminal microbes of goat were capable of withstanding up to 4% tannin of Acacia nilotica pods in the TMR without affecting in vitro nutrient digestibility.

• Journal of Korean Society of Water and Wastewater
• /
• v.31 no.5
• /
• pp.441-445
• /
• 2017

This study aimed to investigate growth rate and nutrient consumption of Chlorella vulgaris according to different light irradiation interval. Applied light irradiation intervals were 12 hr, 4 hr, 1 hr, and 1 min. The light source was flexible LED(Blue:Red=1:1), light intensity was 200 PPFD and Light/Dark cycle was 1:1. As a result, growth rate and nutrient removal efficiencies showed no significant differences depending on the light irradiation interval. Considering the reproduction characteristics of applied microalgae cultures of this study, this is thought to be one of the possible reasons of above results. Because Chlorella vulgaris performs an asexual reproduction and it is known that there is no significant relationship between light irradiation interval and growth rate, including nutrient consumption in case of asexual reproduction.

• Journal of Korean Society on Water Environment
• /
• v.28 no.5
• /
• pp.663-668
• /
• 2012

The feasibility of enhancing biological nutrient removal from an industrial wastewater was tested with food waste leachate and sugar liquid waste as external carbon sources. Long term influences of adding external carbon sources were investigated to see how the biological nutrient removal process worked in terms of the removal efficiency. The addition of the external carbons led to a significant improvement in the removal efficiency of nutrients: from 49% to approximately 76% for nitrogen and from 64% to around 80% for phosphorus. Approximately, 20% of the removal nitrogen was synthesized into biomass, while the remaining 80% was denitrified. Though the addition of external carbon sources improved nutrient removal, it also increased the waste sludge production substantially. The optimal observed BOD/TN ratio, based on nitrogen removal and sludge production, was around 4.0 in this study.

• Korean Journal of Agricultural Science
• /
• v.51 no.2
• /
• pp.179-186
• /
• 2024

Soybean meal (SBM) is a high-protein plant product commonly used as the primary protein source in pig diets. However, its price has been steadily rising, prompting us to search for cost-effective, high-yield protein sources. This experiment aimed to assess the effects of partial replacing SBM with 6% of palm kernel meal (PKM), lupin kernel (LK), rapeseed meal (RSM), and distillers dried grains solubles (DDGS) on the growth performance, nutrient digestibility, and fecal scoring in growing pigs. A total of 200 (Yorkshire × Duroc) growing pigs with an initial weight of 34.83 ± 1.38 kilograms were utilized in this research for 29 days. All pigs were randomly assigned to one of five dietary treatments based on their gender and initial body weight, Each treatment consisted of 10 replicates with 2 barrows and 2 gilts per pen. The dietary treatments were as follows: control (CON), a corn-SBM-based diet; and basal diet supplemented with 6% of different plant byproducts (PKM, LK, RSM, and DDGS) Adding 6% of RSM to the basal diet showed slightly higher daily gain (2.520 > 2.513) and there was no difference observed on the nutrient digestibilty and fecal score. Replacing soybean meal with different plant byproducts has no adverse effect on growth performance, nutrient digestibility, and fecal score.

• Asian-Australasian Journal of Animal Sciences
• /
• v.23 no.1
• /
• pp.82-89
• /
• 2010

An experiment was conducted to determine the influence of dietary inorganic (copper sulfate) and organic (copper proteinate) forms of copper and energy level on performance and nutrient utilization of broiler chickens. Two hundred day-old commercial Vencobb broiler chicks were purchased and randomly distributed to 20 cages of 10 birds each. These replicates were randomly assigned to one of five treatments in a (($2{\times}2$)+1) factorial arrangement. These two factors were sources of Cu ($CuSO_{4}$ vs. Cuproteinate) and dose of Cu supplements (200 mg and 400 mg/kg dietary dry matter) and the control (no supplemental Cu). After the starter period (up to 3 weeks), from d 22 onwards another factor i.e. energy at two levels (2,900 vs. 2,920 kcal/kg diet) was introduced with the previous factorial arrangements by subdividing each replicate into two equal parts, for two energy levels, without disturbing the dose and source of Cu supplement. Cu-salt supplementation linearly increased (p<0.01) live weight (LW), live weight gain (LWG) and feed conversion ratio (FCR) at 3 weeks, whereas cumulative feed intake (CFI) was unaffected (p>0.05). LWG and FCR were higher (p<0.01) in Cu-proteinate supplemented birds compared to $CuSO_{4}$ supplementation. A linear dose response (p<0.01) of Cu was found for the performance of broiler chickens. Birds having a higher energy level in the finisher stage increased (p<0.01) LWG and FCR. Cumulative feed intake was similar (p>0.05) across the groups up to the 5th week. Cu-proteinate increased performance of broiler chickens compared to $CuSO_{4}$. Dose of supplemental Cu-salt irrespective of source showed a linear response (p<0.01) for performance. Supplementation of Cu-proteinate increased metabolizability of DM (p<0.01), NFE (p<0.05), total carbohydrate (p<0.01) and OM (p<0.01) at the starter period. Increased dose of Cu-salt linearly increased (p<0.01) metabolizability of DM, CP, CF, NFE and OM. Higher energy level in the diet improved DM (p<0.05), EE (p<0.01), NFE (p = 0.01), total carbohydrate (p<0.01) and OM (p<0.01) metabolizability. Cu-proteinate supplementation showed better nutrient utilization compared to CuSO4. Dose of Cu linearly increased DM, CP, EE, NFE, total carbohydrate and OM metabolizability. CF metabolizability was unaffected (p>0.05) among the treatments. In conclusion, dietary supplementation of Cu-salt more than the requirement may improve performance and nutrient utilization in broiler chickens even with a high energy finisher diet. Cu-proteinate showed better performance and nutrient utilization compared to $CuSO_{4}$.

• Microbiology and Biotechnology Letters
• /
• v.31 no.1
• /
• pp.83-89
• /
• 2003

2,4-Dichlorophenoxyacetic acid (2,4-D) as a widely used herbicide has caused serious environmental problems because of its difficult decomposition in nature. We isolated the strain capable of metabolizing 2,4-D as sole carbon and energy source by an enrichment culture technique from the 2,4-D contaminated soil collected at orchard in Gwangju, Korea. This strain was identified tentatively as Aeromonas sp. NOH2. With this strain, we established the response surface methodology (Box-Behnken Design) to optimize the principle parameters for maximizing biodegradation of 2,4-D such as culture pH, temperature, and nutrient concentration in liquid batch culture. The ranges of parameters were obtained from preliminary works done at our laboratory and chosen as 5.5, 6.5, and 7.5 for pH, 25, 30, and $35^{\circ}C$ for temperature, and 5, 20, and 35 g/1 nutrient concentration. Initial concentration of 2,4-D was 500 ppm and nutrient source was tryptic soy broth. The experimental data were significantly fitted to a second order polynomial equation using multiple regression. The most important parameter influencing 2,4-D degradation and biomass production was nutrient concentration. For 2,4-D degradation, the optimum values of pH and temperature, and nutrient concentration were obtained at pH (6.5), temperature (31.8 to $32.1^{\circ}C$), and nutrient concentration (29.6 to 30.1.0 g/1).