• Molecules and Cells
• /
• 제45권9호
• /
• pp.649-659
• /
• 2022

A long-term energy nutritional imbalance fundamentally causes the development of obesity and associated fat accumulation. Lysosomes, as nutrient-sensing and lipophagy centers, critically control cellular lipid catabolism in response to nutrient deprivation. However, whether lysosome activity is directly involved in nutrient-induced fat accumulation remains unclear. In this study, worm fat accumulation was induced by 1 mM glucose or 0.02 mM palmitic acid supplementation. Along with the elevation of fat accumulation, lysosomal number and acidification were also increased, suggesting that lysosome activity might be correlated with nutrient-induced fat deposition in Caenorhabditis elegans. Furthermore, treatments with the lysosomal inhibitors chloroquine and leupeptin significantly reduced basal and nutrient-induced fat accumulation in C. elegans. The knockdown of hlh-30, which is a critical gene in lysosomal biogenesis, also resulted in worm fat loss. Finally, the mutation of aak-2, daf-15, and rsks-1 showed that mTORC1 (mechanistic target of rapamycin complex-1) signaling mediated the effects of lysosomes on basal and nutrient-induced fat accumulation in C. elegans. Overall, this study reveals the previously undescribed role of lysosomes in overnutrition sensing, suggesting a new strategy for controlling body fat accumulation.

• Asian-Australasian Journal of Animal Sciences
• /
• 제16권4호
• /
• pp.534-540
• /
• 2003

This study was conducted to investigate the effect of supplementation of galacto-oligosaccharides (GOS) on methane emission, nutrient digestibility, energy utilization and blood metabolites by Holstein cows fed silages. In two sequential digestion and respiratory trials, two non-lactating Holstein cows were arranged to a balanced incomplete block design. Experimental diets consisted of two silage types; orchardgrass (Dactylis glomerata L.) based silage (OS), mixed silage (orchardgrass based silage and alfalfa (Medicago sativa L.) silage) (MS), while two GOS levels were without supplementation (0) and 2% of dry matter intake supplementation (2). Four combination diets were OS-0, OS-2, MS-0 and MS-2. Significant effects of silage types and GOS supplementation levels were not observed for DM and OM intake. Whereas the digestibility of OM, NDF and ADF was significantly (p<0.05) higher in cows fed OS with and without GOS compared cows fed MS diets. As percentage of GE intake, fecal energy loss for OS diets was significantly (p<0.05) declined than for MS diets. In contrast, cows fed MS diets had lower (p<0.05) urine energy loss as a proportion of GE intake compared to OS diets. Energy loss as CH4 and heat production was numerically increased when cows fed both OS and MS with GOS supplementation. Compared to OS, CH4 emission in cows fed MS was numerically decreased by 10.8 %. Methane conversion ratio (energy loss as CH4 per unit of GE intake) for OS-0, OS-2, MS-0 and MS-2 were 7.1, 7.2, 6.8 and 7.0, respectively. Plasma of glucose and urea-N concentration were significantly (p<0.05) elevated from 1 h to 6 h after feeding, otherwise total protein in plasma was declined (p<0.01) at 6 after feeding.

• 한국환경농학회지
• /
• 제28권1호
• /
• pp.38-46
• /
• 2009

Livestock manure is an important source of nutrients for crop production. However, farmers typically do not know the exact nutrient values for livestock manure. In many instances, manure has been viewed as a waste, and as a result it is applied close to the source resulting in over application of nutrients. Thus, the goal of nutrient application has often been applied to reduce the application expense rather than to maximize crop income. This results in wasted money and potentially negative impacts on water quality. Several livestock manure management scenarios were created based on agronomic nutrient requirements using the Utilization of Animal Manure as a Plant Nutrient (AMANURE) software to investigate water quality impacts with the National Agricultural Pesticide Risk Analysis (NAPRA) WWW modeling system. Application of manure at agronomic rates can result in high nitrate-nitrogen losses for some soil types, especially when applied in late fall. The application of manure at an agronomic rate does not necessarily equate to adequate water quality protection, and farmers must take care applying manure at agronomic rates, because nitrate-nitrogen loss potential varies spatially and temporarily. Nutrient loss probability maps for Indiana at 5%, 10%, 25%, and 50% values were created to demonstrate potential water quality impacts when livestock manure is applied to cropland at agronomic rates. The NAPRA WWW system coupled with AMANURE can be used to identify site-specific livestock manure management plans that are environmentally sound and agronomically appropriate.

• Clinical and Experimental Pediatrics
• /
• 제52권12호
• /
• pp.1327-1336
• /
• 2009

Increasing numbers of children and adolescents prefer undertaking physical exercise to overcome overweight or obesity. Children and adolescents are in the growth stage and require adequate nutrient supply. More calories and nutrients are required especially when they are engaged in physical exercise. Exercise is the only means to increase lean body mass and decrease body fat, but adequate nutrient supply is also essential. Lack of adequate nutrient supply causes muscle mass loss, menstruation irregularity, reduced bone density, fatigue, or frequent injury in children undertaking physical exercise. Here, I have introduced some guidelines on the nutrient requirement for child and adolescent athletes.

• Journal of Nutrition and Health
• /
• 제33권3호
• /
• pp.241-249
• /
• 2000

The bone mineral density(vertebrae Ll-4, femoral neck, Ward's triangle and trochanter) of 160 healthy adult women aged 29-45 was measured and general characteristics, anthropomentric measurements, and dietary intake were also studied to determine the main factors affecting the bone mineral density(BMD) of adult women. The nutrient intake of the subjects was adequate to RDA level except energy, Ca, Fe, and vit A. The BMD of vertebrae L24 showed significant positive association with nutrient intake such as animal protein, P, Ca(animal) and Fe(animal). The BMD of the femoral neck correlated significantly with anthropometric measurements such as height, weight, waist circumference, hip circumference, body fat and body mass index. The BMD of both sites(vertebrae L24 and femoral neck) were significantly related to vertebrae L24 was daily Ca intake and that of femoral neck was daily energy expenditure. In conclusion, adequate nutrient intake, especially protein, P, Ca and Fe as well as increases in physical activity were suggested to prevent the loss of bone mineral density in adult women.

• Asian-Australasian Journal of Animal Sciences
• /
• 제13권7호
• /
• pp.948-956
• /
• 2000

The effect of high and low level of feed intakes on nutrient digestibility, nutrient losses through methane, energy and protein utilization by goats fed on alfalfa (Medicago sativa L.) pellets based diets was investigated in this study. Twelve castrated Japanese goats were employed in two subsequent digestion and metabolism trials. The goats were divided into three groups, offered three diets. Diet 1 consisted of 100% alfalfa pellet, Diet 2 was 70% alfalfa pellet and 30% corn, and Diet 3 was 40% alfalfa pellet and 60% corn. The two intake levels were high (1.6 times) and low (0.9 times) the maintenance requirement of total digestible nutrients (TON). Rumen ammonia nitrogen ($NH_3$-N) level of Diet 1 was lower (p<0.001) compared to Diets 2 and 3, but the values were always above the critical level (I50 mg/liter), The pH values of rumen liquor ranged from 6.02 to 7.30. Apparent digestibility of nutrient components did not show differences (p>0.05) between the two intake levels but inclusion of corn significantly altered the nutrient digestibility. Diet 3 had highest (p<0.001) dry matter (DM), organic matter (OM), ether extract (EE) and nitrogen fee extract (NFE) digestibility followed by the Diet 2 and Diet 1. The crude protein (CP) digestibility values among the three diets were in a narrow range (70.1 to 70.8%). Crude fiber (CF) digestibility for Diet 3 was slight higher (p>0.05) than that for other two diets. When alfalfa was replaced by corn, there were highly significant (p<0.001) increases in DM, OM, EE and NFE apparent digestibility and a slight increase in the CF digestibility (p>0.05). There were no differences (p>0.05) in energy losses as methane ($CH_4$) and heat production among the diets but energy loss through urine was higher for the Diet 1. The total energy loss as $CH_4$ and heat production were higher for the high intake level but the energy loss as $CH_4$ per gram DM intake were same (0.305 kcal/g) between the high and low intake level. Retained energy (RE) was higher for Diet 3 and Diet 2. Nitrogen (N) losses through feces and urine were higher (p<0.001) for Diet 1. Consequently, N retention was lower (p>0.05) for Diet 1 and higher in Diets 3 and 2. It is concluded that inclusion of corn with alfalfa increased the metabolizable energy (ME) and RE, and retained N through reducing the energy and N losses. The high level of intake reduced the rate of nutrient losses through feces and urine.

• 한국토양비료학회지
• /
• 제42권4호
• /
• pp.231-238
• /
• 2009

본 연구는 고랭지 경사밭에서 발생하는 유거수량, 토양유실, 영양물질 유출량을 감소시키는 완충식생대의 효과를 연구하기 위하여 수행하였다. 시험에 사용된 포장의 토양은 운교통이고, 가로 2.5m, 세로 20m인 무저 lysimeter에서 시험하였다. 연구포장은 경사율 17%를 가진 경사밭이며, 배추를 재배하였다. 완충식생대로 이용된 초종은 Rye(Secale cereale L.), Tallfescue(Festuca arundinacea Schreb) Orchardgrass(Dactylis glomerata L.)이다. 이들은 경사밭 아래 부분에 조성되었다. 호밀 식생대의 폭은 1m, 2m, 4m를 두었고, Orchard grass와 Tall fescue는 2m로 설정하였다. 완충식생대를 조성하기 위해 각 초종을 2005년 9월에 파종하였고, 배추는 2006년 6월에 정식하였다. 토양유실량, 유거수량, 영양물질 유출량의 측정은 2006년 6월부터 8월까지 수행하였다. 유거수량, 토양유실량 및 영양물질 유실량은 강우가 집중된 7월에 가장 높았다. 유거수를 감소시키는 완충식생대의 효과를 대조구와 비교해 볼 때, 2m로 폭이 같은 완충식생대에서 호밀은 3%, Orchard grass는 1%, Tall fescue는 2% 저감 효과가 있었다. 폭이 다른 호밀 완충식생대간의 비교에서, 1m는 1%, Rye 2m는 3%, Rye 4m는 13%의 저감효과가 나타나 Rye 4m의 효과가 가장 좋았다. 토양유실저감측면에서 대조구와 비교할 때, 2m 완충식생대 중 호밀식생대는 59%, Orchard grass 46%, Tall fescue 28% 토양유실을 줄이는 것으로 나타나 같은 폭에서 호밀식생대의 효과가 가장 좋았다. 폭이 다른 호밀 식생대의 비교에서 1m는 62%, 2m는 60%, 4m는 88%의 토양유실 저감 효과가 나타나, 4m의 효과가 토양유실을 저감하는데 가장 좋은 효과를 보였다. 한편, 유거수와 토양에서 발생한 N, P, K 유출 저감에서는 호밀 2m완충식생대에서 각각 54%, 16%, 11%, Orchard grass는 각각 22%, 24%, 22%를, Tall fescue는 10%, 7%, 12% 영양물질유출을 줄였다. 호밀완충식생 중에서 폭이 큰 4m에서 영양물질 유출저감효과가 가장 좋았다. 이 연구의 결과, 유거수량, 토양유실 저감효과는 호밀완충식생대가 다른 초종의 식생대보다 좋아서 유거수, 토양유실 그리고 영양물질 유출 저감을 위해 폭이 큰 호밀완충식생대의 설치가 효과적이라 생각된다.

• 대한한의학원전학회지
• /
• 제25권4호
• /
• pp.23-38
• /
• 2012

Objective : Generally speaking Reverting yin disease pattern(厥陰病) is the last step in cold damage(傷寒). Therefore recognized Yin cold disease(陰寒病) is increasing, and resist action One Yang qi(一陽) began to creep into body. But Ke Qin(柯琴) have a different way of thinking that Reverting yin disease pattern connected with the loss of Liver's function. Liver qi depression(肝鬱) make a ministerial fire(相火), and it make a nutrient and blood insufficiency(營血不足). Method : I will try to describe the Sanghanlun's Reverting yin disease pattern through the Ke-qin's JueyinbingJie(厥陰病解), and I would like to point out that the exact meaning of Reverting yin(厥陰) is connected with Liver's ministerial fire. Result : Ke Qin's JueyinbingJie explained the Reverting yin disease pattern was connected with Liver(肝), and according to Six qi theory(六氣學說) connected with ministerial fire, and according to meridian and Collateral theory(經絡學說) connected with closing referring to inward actions(闔) among the Opening closing and pivot(關闔樞). Conclusion : Ke Qin was recognized that Reverting yin disease pattern have relevance to the loss of Liver's function. In other world, It is connect with soothe the liver and purge fire(疏肝瀉火) and nutrient and blood insufficiency(營血不足).

• Journal of Forest and Environmental Science
• /
• 제26권1호
• /
• pp.17-23
• /
• 2010

The present study was conducted to quantify wood and leaf litter decomposition and nutrient release of a dominant tree species, Tectona grandis Linn. F. in a tropical dry deciduous forest of Rajasthan, Western India. The mean relative decomposition rate was maximum in the wet summer and minimum during dry summer. Rainfall and its associated variables exhibited greater control over litter decomposition than temperature. The concentrations of N and P increased in decomposing litter with increasing retrieval days. Mass loss was negatively correlated with N and P concentrations. The monthly weight loss was significantly correlated (P < 0.05) with soil moisture and rainfall in both wood and leaf litter. Tectona grandis was found to be most suitable tree species for plantation programmes in dry tropical regions as it has high litter deposition and decomposition rates and thus it has advantages in degraded soil restoration and sustainable land management.

• Journal of Animal Science and Technology
• /
• 제66권1호
• /
• pp.115-124
• /
• 2024

This study investigated the impact of clay mineral 'illite' (IL) on the growth performance, apparent total tract digestibility, and meat-carcass grade quality in growing-finishing pigs. One hundred fifty pigs were divided into two groups with fifteen pens/group and five pigs/pen, a control group that was fed with a corn-soybean meal-based diet and IL treated group were fed a meal-based diet supplemented with 0.5% IL. Compared to the control, IL supplementation increased average daily feed intake (ADFI) and reduce gain to feed ratio (G:F) in the pigs during days 43 to 70 and 99 to 126 and increased dry matter during days 42 and 126, and backfat thickness on day 98 (p<0.05). The drip loss was reduced on day 7, meat firmness tended to increase with dietary IL supplementation. In summary, dietary 0.5% IL supplementation improved ADFI, nutrient utilization of dry matter, and firmness and reduced G:F and drip loss of growing-finishing pigs.