• Title/Summary/Keyword: Nutrient component

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Studies on the Chemical composition of Zostera marina ("거머리 말"의 화학성분에 관한 연구)

  • 박명삼
    • Journal of Plant Biology
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    • v.12 no.1
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    • pp.1-6
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    • 1969
  • General components, free sugars, and free amino acids about Zostera marina which is much distributing and producing along the coastlines of Korea have been separated and identified through Ion exchange resin and Paper chromatography method. So distrigushed are sugars contained that this was quantitated by Bertrand method. 1) The result of the general component analysis of Zostera marina shows that there are much free sugars. 2) According to Bertrand method sugar containment quantitated from terrestrial stem and subterranean stem on Po-hang and Ko-hung areas is much more in terrestrial stem than subterranean stem. 3) Seven kinds of sugars are used in experiments(Table 2) among there glucose, fructose and sucrose which keep the sweetness are very much contained. 4) Shown in Fig. 3 ten kinds of free amino acids inspected from experimental liquid by Paper chromatography are separated, which are known, and one spot which in unknown is additionally separated, and in which four kinds of essential amino acid to nutrient are contained.

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Development of Low-serum Medium(LSM) for Mouse-mouse Hybridoma Part I. A Study of the Role of Serum Components Using a Serum Model (Hybridoma 배양을 위한 저혈청 배지의 개발 제1부 : 혈청 역할모델을 이용한 혈청 성분의 역할 연구)

  • 제훈성;최차용
    • KSBB Journal
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    • v.7 no.2
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    • pp.92-95
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    • 1992
  • A model for the role of serum was proposed to develop a low serum medium for the large scale cu1ture of mammalian cell. The strategy of medium development adopted in this study facilitated the understanding of the role being carried out by the serum in the culture of hybridoma KAl12 cell line. In this model, the serum components were divided into two main groups : the first group encompasses the nutrient factors that determine the maximum cell density and the second group includes the growth factors that regulate the cell growth rate, The model prediction was compared with the experimental results. The model enabled us to find out several useful aspects of medium composition for cell growth. 1) One particular component in the basal medium became limiting factor when serum concentration level was more than 7%. 2) The growth regulating factors and nutrient factors limited the cell growth at 3% and 5% serum concentration levels respectively.

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Production of poly-$\beta$-hydroxybutyric acid(PHB) from Liquefied Natural Gas using an Obligatory Methanotroph Methylosinus trichosporium OB3b (메탄자화균 Methylosinus trichosporium OB3b를 이용한 액화 천연가스로부터 poly-$\beta$-hydroxybutyric acid(PHB)의 생산)

  • 황재웅;박성훈
    • KSBB Journal
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    • v.11 no.2
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    • pp.246-253
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    • 1996
  • An obligatory methanotroph Methylosinus trichosporium OB3b was cultivated for the production of poly-${\beta}$-hydroxybutyric acid(PHB) in shake-flask using liquefied natural gas(LNG) as the sole source of carbon and energy. The maximal specific growth rate decreased by 40% using LNG compared with that obtained with pure methane. This is attributed to the inhibition by ethane and propane presents in the LNG as impurities. For the production of PHB, two-stage culture separating the production stage from the growth stage was carried out. PHB accumulation was observed after switching nutrient-sufficient to nutrient-limited condition of non-carboneous component (NO3-, PO43-, K+, Na+, Fe2+, or Mg2+). The limitation of K+ or Mg2+ resulted in relatively high PHB content, but the highest content was obtained by nitrate limitation. The optimal pH and temperature for PHB accumulation was 7.0 and $30^{\circ}C$. Under the optimal condition the maximal PHB content was about 45% after 4-day cultivation.

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Component Comparions of the Nutrient Composition of Lentinus Edodes based of Harvest Period (표고버섯의 채취 시기 및 부위별 영양성분 함량 비교)

  • 박홍주;이성현;백오현;조수묵;조용식
    • The Korean Journal of Community Living Science
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    • v.15 no.3
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    • pp.107-112
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    • 2004
  • Many studies have showen that Letinus edodes have a reducing effects on blood pressure and lipid levels in animals with high blood pressure and hyperlipidemia. But the only cap has been on the market as goods and the stipe has been wasted. The price is very variable and is based on harvest time and the part of the plant currenty, there is not much information about the nutritional composition and bio-fuctional effects of Letinus edodes based on harvest time and part. Some farmers have been selling the powder seasoning by drying and pulverizing Letinus edodes including stipe to develop value added, late harvested Letinus edodes and its stipe these days. This study was conducted to investigate and compare the nutritional composition of Letinus edodes harvested at different times (early: E, late: L) and separated by part (cap: C, stipe: S). The 4 kinds of materials were named as early harvested cap (EC) and stipe (ES), and late harvested cap (LC) and stipe (LS) samples were obtained from Mungyeong City Agricultural Technology & Extension Center. The composition analysis of the nutrients in the 4 materials was conducted by using the AOAC method. The proximate components (moisture, protein, fat, fiber, ash, and carbohydrates), minerals and vitamin content were measured. Nutritional composition of Lentinus edodes did not show any significant difference between early and late harvested mushrooms. Nutritional compositions were different according to part. The results demonstrate that the late harvested stipe can also be used as a quality food material.

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Assessing Changes in Selected Soil Chemical Properties of Rice Paddy Fields in Gyeongbuk Province

  • Park, Sang-Jo;Park, Jun-Hong;Won, Jong-Gun;Seo, Dong-Hwan;Lee, Suk-Hee
    • Korean Journal of Soil Science and Fertilizer
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    • v.50 no.3
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    • pp.150-161
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    • 2017
  • This study was conducted with the data of monitoring on soil chemical properties of rice paddy soils in Gyeongbuk Province. The selected soil chemical properties were analyzed every 4 year from 1999 to 2015. The soil pH measured in 2015 was higher than pH 6.0, which was 0.3-0.4 pH unit higher than data until 2007 survey year. The mean content of organic matter was greater than $24g\;kg^{-1}$ since 2003, but 35% of soil samples remained below the recommended level ($20-30g\;kg^{-1}$) in 2015. The mean concentration of available phosphate was maintained at $40mg\;kg^{-1}$ higher than the upper recommendation level ($80-120mg\;kg^{-1}$), and more than 40% of paddy soils tested were found to have less than the recommendation level during the survey period. The exchangeable K concentration ranged from 0.25 to $0.39cmol_c\;kg^{-1}$. Exchangeable Ca showed an average at the optimum range ($5.0-6.0cmol_c\;kg^{-1}$) during the monitoring period. Exchangeable Mg decreased linearly ($0.02cmol_c\;kg^{-1}\;year^{-1}$) from $1.55cmol_c\;kg^{-1}$ as of 1999 to below the lower level of the recommendation range ($1.5-2.0cmol_c\;kg^{-1}$). The amount of available $SiO_2$ was increased significantly from 2011 to over the recommendation level (${\geq}157mg\;kg^{-1}$). It was revealed that the soil chemical properties of rice paddy fields was influenced by topology, soil texture, type and region as result of principal component analysis or cluster analysis. Therefore, an assessment on chemical properties of rice paddy soils should be performed to consider various soil physical conditions and agronomic practices such as fertilization, cropping system, and so on. Because of the high variability of nutrient levels across Gyeongbuk Province, nutrient management based on soil fertility test is required by respective farm land unit.

Long-term Application Effect of Silicate Fertilizer on Soil Silicate Storage and Rice Yield

  • Kim, Myung-Sook;Park, Seong-Jin;Lee, Chang-Hoon;Ko, Byong-Gu;Yun, Sun-Gang
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.6
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    • pp.819-825
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    • 2016
  • Monitoring of soil fertility and crop productivity in long-term application of silicate fertilizers is necessary to use fertilizers efficiently. This study was conducted to investigate effects of continuous application of silicate fertilizer for rice cultivation from 1969 to 2014. The treatments were no silicate fertilizer treatments (N, NC, NPK, and NPKC) and silicate fertilizer treatments (N+S, NC+S, NPK+S, and NPKC+S). The 46-yr input of $2\;ton\;ha^{-1}yr^{-1}$ of silicate fertilizer increased pH 0.6 ~ 1.1 and exchangeable Ca $2.0{\sim}2.4cmol_c\;kg^{-1}$ in silicate fertilizer treatments (N+S, NC+S, NPK+S, and NPKC+S) compared with no silicate fertilizer treatments (N, NC, NPK, and NPKC) because silicate fertilizer included Ca component. Also, available silicate concentrations of silicate fertilizer treatments (N+S, NC+S, NPK+S, and NPKC+S) increased $169mg\;kg^{-1}$ compared to no silicate fertilizer treatments. In Period II ('90~'14), the mean annual Si field balance varied from 62 to $175kg\;ha^{-1}yr^{-1}$ in silicate fertilizer treatments, indicating continuous accumulation of soil Si. Silicon uptake and grain yield of rice had greater differences between N treatment and N+S treatment than other treatments. This showed that the application of silicate fertilizer had greater effect in nutrient-poor soils than in proper nutrient soils. Thus the application of silicate fertilizer led to improvement the fertility of soil and increasement of rice production for the lack of soil nutrients.

Variation of Ecological Niche of Quercus serrata under Elevated $CO_2$ Concentration and Temperature ($CO_2$ 농도 및 온도 상승에 의한 졸참나무의 생태적 지위 변화)

  • Cho, Kyu-Tae;Jeong, Heon-Mo;Han, Young-Sub;Lee, Seung-Hyuk
    • Korean Journal of Environmental Biology
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    • v.32 no.2
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    • pp.95-101
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    • 2014
  • In order to investigate effects of elevated $CO_2$ concentration and temperature on the ecological niche of Quercus serrata in Korea. We divided experimental condition in the greenhouse that are control (ambient condition) and treatment with elevated $CO_2$ (approximately 1.6 above than control) and increased air temperature (approximately $2.2^{\circ}C$ above than control). We measured twenty kind characters of seedlings and calculated the ecological niche breadth. As a result, the ecological niche breadth, treatment was widened in the light gradient than the control, was narrowed in the moisture and nutrient gradient. This is may be predicted when the global warming progress, Q. serrata is increases resistance to light environment, and decrease resistance to moisture and nutrient environment. According to the principal component analysis (PCA), control and treatment were arranged based on factor 1 and 2 in each environment gradients. Ecological response is involved variety characters. Among them, indicating that Characters of production is involved in many a parts.

Dietary protease improves growth rate and protein digestibility of growing-finishing pigs

  • Lee, Jeong Jae;Choe, Jeehwan;Kang, Joowon;Cho, Jin Ho;Park, Sangwoo;Perez-Maldonado, Rider;Cho, Jee-Yeon;Park, Il-Hun;Kim, Hyeun Bum;Song, Minho
    • Journal of Animal Science and Technology
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    • v.62 no.3
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    • pp.313-320
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    • 2020
  • This research was performed to investigate the hypothesis that dietary mono-component protease (PRO) might improve growth performance, nutrient digestibility, and carcass characteristics of growing-finishing pigs. A total of eighty-four pigs [Duroc × (Landrace × Yorkshire), 25.3 ± 2.16 kg initial body weight] were randomly assigned to three dietary treatments (7 replicates/treatment; 2 barrows and 2 gilts/replicate) in a randomized complete block design (block = sex). The dietary treatments were prepared as follows; (1) a positive control (PC) as a typical growing-finishing diet based on corn and soybean meal, (2) PC added with 0.015% of PRO (PCPRO), and (3) a negative control (NC) added with 0.015% of PRO (NCPRO). The NC had a lower concentration of crude protein (CP) compared with PC. The PRO was a commercial product that contained 75,000 protease units/g and derived from Nocardiopsis prasina produced in Bacillus licheniformis. Dietary treatments were offered to pigs during growing and finishing periods. Measurements were growth performance, apparent total tract digestibility (ATTD) of nutrients, and carcass characteristics. The PCPRO and/or NCPRO increased average daily gain (ADG) and gain to feed ratio (G:F) during growing (p < 0.10), finishing (p < 0.05), and growing-finishing periods (p < 0.10) compared with PC. Furthermore, pigs fed PCPRO and NCPRO had higher (p < 0.05) ATTD of CP and energy during growing and/or finishing periods than those fed PC. In conclusion, the supplementation of PRO in diets improved growth performance and protein digestibility of growing-finishing pigs.

School Dietitians′ Perception on Nutrition Labelling of the Processed and Packaged Foods in Incheon (인천지역 학교급식 영양사의 가공포장식품 영양표시에 대한 인식)

  • 정혜열;장경자
    • Korean Journal of Community Nutrition
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    • v.9 no.5
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    • pp.636-643
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    • 2004
  • The purpose of this study was to investigate school dietitians' perception on nutrition labelling of processed and packaged foods in the Incheon area. This study was carried out through a self-administered questionnaire and the subjects were 203 school dietitians. The results were summarized as follows. Most of the subjects checked nutrition labeling of processed and packaged foods when they purchased these foods for school lunch. However, the main reason for their checking nutrition labeling of processed and packaged foods was not for nutrition but for food safety. There were significant differences in the subjects' understanding and trust on nutrition labeling of processed and packaged foods by employment school. The scores of the subjects' satisfaction, understanding and trust on nutrition labeling of processed and packaged foods were not high. There were significant differences in the subjects' perception and concern about content information on nutrition labeling of domestic processed and packaged foods by employment school. Most of the subjects answered that it is almost necessary or very necessary to implement nutrition labelling of processed and packaged foods. Also their most preferred method of nutrition labelling was to label nutrient content. As the most important nutrient and functional component necessary for nutrition labelling, they answered energy, mineral such as calcium and iron, protein, total fat, cholesterol, vitamin, sodium and dietary fiber in order. As the major prerequisite task for the implementation of nutrition labelling, they answered formulation of nutrition labelling-related rules, and education and motivation for consumer, Therefore, it is necessary to activate the mandatory nutrition labelling of the processed and packaged foods and to prepare its consumer education program for school dietitian.

Synergistic Effects of Arbuscular Mycorrhizal Fungi and Plant Growth Promoting Rhizobacteria for Sustainable Agricultural Production

  • Ramasamy, Krishnamoorthy;Joe, Manoharan Melvin;Kim, Ki-Yoon;Lee, Seon-Mi;Shagol, Charlotte;Rangasamy, Anandham;Chung, Jong-Bae;Islam, Md. Rashedul;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.4
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    • pp.637-649
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    • 2011
  • Soil microorganisms play a major role in improving soil fertility and plant health. Symbiotic arbuscular mycorrhizal fungi (AMF) form a key component of the soil microbial populations. AMF form a mutualistic association with the host plant and exert a positive influence on its growth and nutrient uptake. The establishment of mycorrhizal symbioses with the host plant can positively be influenced by plant growth promoting rhizobacteria through various mechanisms such as increased spore germination and hyphal permeability in plant roots. Though there are evidences that combined interactions between AMF and PGPR can promote the plant growth however mechanisms of these interactions are poorly understood. Better understanding of the interactions between AMF and other microorganisms is necessary for maintaining soil fertility and enhancing crop production. This paper reviews current knowledge concerning the interactions between AMF and PGPR with plants and discusses on enhanced nutrient availability, biocontrol, abiotic stress tolerance and phytoremediation in sustainable agriculture.