• 한국환경복원기술학회지
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• 제2권2호
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• pp.53-61
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• 1999

This study was conducted to determine the optimal collecting time and methods of utilization of forest topsoil as a seed-bank source for revegetation materials of slopes. From April to November of 1998, nursery seedbeds in the greenhouse of Seoul National University were seeded with mixtures of forest topsoil and seeds. The nursery seedbeds were treated to evaluate the effect of depths of forest topsoil and seeding amounts. Seasonal experiments with $3{\times}3$ factorial design were conducted in April, June and August. More than 40 seedlings/$m^2$ were observed as the naturally-emerged species in the plot, treated with the top-layer forest soil within the depth of 10cm. However, it was found, as seeding more seeds, the number of appearance of the naturally-emerged species reduced because of the heavy competition between the seeded and the naturally-emerged. According to the results of this research, it is recommended that seeding amount should be no more than 1,000 seeds/$m^2$ to avoid the severe competition. Also it was observed that the forest topsoil collected in spring(April) is better than that collected in summer. The more species of the naturally-emerged were found in the forest topsoil collected in spring. Forest-topsoil-mixed-materials as a seed-bank source could significantly increase the plant diversity and productivity. Effective use of forest topsoil as a seed-bank source may become a valuable tool in future restoration of disturbed slopes for promoting plant community diversity and recycling of spoiled-soils from the slopes.

• The Plant Pathology Journal
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• 제22권1호
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• pp.21-27
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• 2006

We immunized BALB/c mice with purified Cucumber green mottle mosaic virus isolate HY1 (CGMMV-HY1). Through the selection of positive clones that were grown on the HAT medium, four sensitive monoclonal clones (CG99-01, CG99-02, CG99-03, and CG99-04) were selected from 500 Hypoxanthine-guanine phosphoribosyltransferase positive hybridoma cells. Four sensitive clones of CGMMV-HYI were determined as IgM type of the subclass of mouse immunoglobulins Ig group. The titer of monoclonal antiserum against CGMMVHY1 was estimated 1:12,800 by the indirect ELISA. Although monoclonal antibodies (MAbs) from CG99-01 and from CG99-04 cross-reacted with Zucchini green mottle mosaic virus and Kyuri green mottle mosaic virus, MAb from the cell line CG99-03 was highly specific to CGMMV. No MAbs cross-reacted with Cucumber mosaic virus-Fny. Only CG99-04 reacted with Pepper mild mottle virus weakly and CG99-02 reacted with both CGMMV and KGMMV. CGMMV was detected from the rind of watermelon fruit by DAS-ELISA of CGMMV-HY1, but not from the flesh of watermelon. Average seed transmission rate of CGMMV in watermelon was $24\%$ from symptomatic watermelon collected from 5 regions of Gyeongnam province. CGMMV was detected by DAS-ELISA with specific MAb of CGMMVHY1 periodically from root stock, during the sequential process for nursery seedling in Haman. Necrotic spots on cotyledons of root stock seedling progressed to reveal the typical symptomatology on the primary leaves of scion upon grafting. Here, we have established MAb based ELISA system, which could accurately detect CGMMV from watermelon seeds, nursery seedlings, transplants and field samples from greenhouse or open out door field as well.

• 한국응용곤충학회지
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• 제13권1호
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• pp.19-23
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• 1974

전적적으로 널리 재배되고 있는 통일(IR667-98)이 우리나라의 현존 도열병균 Race이 대하여 고도로 저항성을 유지하고 있다. 그러나 통일과 그 자매계통은 1972년 서울대학교 도열병검정밭못자리에서 통일에서 분리한 RaceIA-65에 대해 중도감수성이었다. 1973년에는 IA-65를 접종한 격리밭못자러에서 통일을 비롯한 IR계통은 이 Race를 접종하지 않은 농장못자리보다 현저히 병원성이 높았다. 또한 IA-65에 대한 196 IR 계통의 유묘점정반응은 통일을 포함한 약 $30\%$가 중도감수성이었다. 1973년에 다시 통일을 비롯한인도형 계통에서 주로 분리한 30균주중 12개는 통일이 고도감수성이었던 필리핀 국제미작연구소의 우점 Race 군으로 알려진 IA군으로 판별되었다. Race IA군도 유묘검정에서 통일에 병원성이 있었다. 이와같이 통일 및 IR 계통에 병원성인 Race IA 65 및 IA군은 한국을 비롯한 일본형 벼재배지대에서 보고된 바 없는 새로운 것으로 생각된다. 우리나라에 현존하는 몇 가지 Race가 통일에 병원성을 보이고 있고 더 나아가서 이들 Race가 일반 농도포장에서 만연될지도 모르므로 통일에 대한 도열병의 반응이 계속 추구되어야 한다.

• 한국조경학회지
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• 제28권1호
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• pp.48-53
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• 2000

This study was carried out to investigate the growth characteristics and propagation methods of Prunus species as woody landscape plants. Both the flowering time and the survival rate of grafting were investigated. The results are obtained as follows: The total of flowering period of Prunus species in Kyonggi province area lasted for about 26 days. The flowering time of the species overlaps. These observations suggest that the possibility of interspecific pollination among Prunus species is very high in the kyonggi province area. The total number of flowers in the species in area was in as follows decreasing order : (1) Prunus yedoensis >Prunus pendula var. ascendens > Prunus subhitella > Prunus sesrulata for. fugenzo > Prunus leveillenana var. pendula. The number of carpels in each flower ranged from 0.3 for Prunus subhirtella to 1.8 for Prunus serulata for. fugenzo. In the caseof Prunus sesrulata for. fugenzo, the carpels appeared to be degenerated and thus losted their function. However, there exist two tyoes of Prunus subhirtella. While one type had normal carpel, the other had the degenerated one. The survival rate of grafting was investigated on May 19. Eighty there percent of the plants survived when the grafting was made in the greenhouse in January whereas the plants grafted in nursery in March survived less in that Prunus suhirtella showed 64%, Prunus leveilleana var. pendula 47%, Prunus sesrulata for. fugenzo 43%, Prunus yedoensis 62% and Prunus pendula var. ascendens 24%, respectively. Therefore, it suggested that high humidity and optimal temperature appeared to incase the survival rate of the grated plants. We therefore propose here that grafting should be done in the greenhouse that both humidity and temperature could be controlled to enhance the efficiency of grafting. This will enable as to perform grafting in winter as well.

• 한국산업보건학회지
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• 제30권3호
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• pp.292-298
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• 2020

Objectives: The aim of this research is to establish Similar Exposure Groups (SEGs) for chemical and biological risk factors that occur in farm work involving 24 tasks among 15 crops. Methods: To categorize SEGs, work type, work environment, and similar tasks for each crop were considered. After confirming the chemical risk factors (pesticides, inorganic dust-total dust and PM₁₀, ammonia, and hydrogen sulfide) and biological factors (organic dust-total dust and PM₁₀, and endotoxins) that occur in the crops and tasks, similar crops and tasks were selected as SEGs. Results: Among chemical risk factors, pesticides was selected for the SEGs, which was categorized by open field, greenhouse, fruit, and specialty crops. For inorganic dust, open field (plowing harrowing, seedling, planting, harvest, and sorting and packing) and specialty crops (plowing harrowing, seedling, planting, and harvest) were selected as SEGs. For ammonia and hydrogen sulfide, livestock (preparation of farm, management of nursery bed, feeding, shipment and manure treatment) were selected as SEGs. For biological risk factors such as organic dust (total dust, PM₁₀) and endotoxins, open field (manure application), greenhouse (plowing harrowing, planting, manure application, and harvest), fruit (manure application), specialty crops (manure application, making furrows, mixing mushroom media, harvest, and sorting and packing), and livestock (preparation of farm, maintaining poultry litter, feeding, shipment and manure treatment) were selected as SEGs. Conclusions: To establish similar exposure groups in agriculture, it is important that the characteristics of each hazard factor are categorized by identifying risk factors occurring by tasks.

• 한국유기농업학회지
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• 제18권1호
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• pp.55-62
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• 2010

'신고' 배 실생묘의 수체 생육 특성에 관한 결과를 통해서, 충분히 부숙된 유기질 비료의 시용은 뿌리 생체중 및 세근의 양이 많아지고 근 활력이 높아졌을 뿐만 아니라 지상부 수체 생육이 좋아지는 효과를 나타냈다. 과원 내 유목묘의 궁극적인 목적은 왕성한 수체 생장으로 과실 생산을 조기에 달성하는 것인데, 본 실험 결과를 통해서 유기질 비료 처리로 초기 과원재배의 궁극적인 목적을 어느 정도 이룰 수 있을 것으로 판단된다. 또한, 건강한 수체 생산으로 응애의 피해에 의한 낙엽률이나 엽 피해도를 감소시키는 결과를 가져와서 병충해에 대한 내성을 제고함을 알 수 있었다. 따라서 토양 내 충분히 부숙된 유기질 복합 퇴비 시용은 빠른 시일 내에 수체 생장과 안정성을 확보할 수 있다는 측면으로, 육묘장이나 기타 과수 실생묘에 이용될 수 있을 것으로 판단된다

• 농업과학연구
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• 제45권2호
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• pp.143-153
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• 2018

Soil characteristics along with various container lengths have an important role in the early survival rate and growth of seedlings by influencing the seedling quality. This experiment was conducted to investigate the effect of container length and different soil mixtures on the growth of poplar in a greenhouse. Two types of soil, homogeneous vs. heterogeneous, were used along with two container lengths (30 vs. 60 cm). The heterogeneous soil was made by dividing 50% vermiculite from a mixture of 25% vermicompost and 25% nursery soil in volume. For the homogeneous soil, the above three soil types were mixed together. Populus euramericana clone cuttings were planted in late April, and then, the growth height, root collar diameter (RCD) and biomass were measured in August. The height of the poplar was not significantly affected by container length and soil type, but the RCD was significantly affected by soil type. Leaf and root biomass was higher at the long container than at the short container for both soil treatments, but stem biomass was lower at the heterogeneous soil than at the homogeneous soil treatment. Root to shoot biomass ratio was higher at the heterogeneous soil treatment than at the homogeneous soil treatment by 12%. In conclusion, heterogeneous soil along with a long container is suitable to increase the carbon allocation into the root.

• 유기물자원화
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• 제25권3호
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• pp.73-78
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• 2017

본 실험의 목적은 토양탄소 격리 기술을 개발하기 위한 바이오차 팰렛 시용에 따른 토양의 이화학성 변화 및 작물 수량 효과에 대해 평가하는 것이다. 처리는 일반적인 영농 방법으로서 대조구, 돈분 팰렛, 바이오차와 돈분 퇴비 혼합 비율별 바이오차 팰렛 시용구 (9:1, 8:2, 6:4, 4:6, 2:8)로서 구성되어 있다. 바이오차 팰렛의 사용량은 상추 재배를 위한 추천 시용량 (330kg/10a)기준으로 혼합 비율에 관계없이 6.6 g/pot이었다. 상추수확 후 상토의 pH, EC, 암모늄태 질소의 농도 변화를 분석하였다. 실험 결과로서 pH는 대조구와 비교하여 돈분팰렛 처리구에서 증가하였지만, 바이오차 팰렛 처리구(4:6 및 2:8)에서 감소하였다. EC는 처리구간에 유의차를 보이지 않았다. 암모늄태 질소 함량은 단지 돈분 팰렛 처리구에서 증가하였지만, 질산태 질소는 모든 처리구에서 감소하였으나, 상추 수량은 대조구와 비교하여 바이오차 팰렛 처리구( 9:1, 8:2 및 4:6)에서 9.5%에서 11.4%가 증가하는 것으로 나타났다. 그러므로 바이오차 팰렛 시용은 상추생육에 저해요인으로 작용하지 않고, 오히려 긍정적으로 나타남으로서, 작물 재배에 있어서 바이오차 펠렛을 시용하게 되면 토양 탄소격리 및 온실가스 완화를 위해서 유용하다고 생각된다.

• The Plant Pathology Journal
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• 제31권2호
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• pp.140-151
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• 2015

Bacillus subtilis B006 strain effectively suppresses the cucumber fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (Foc). The population dynamics of Foc, strain B006 and its surfactin over-producing mutant B841 and surfactin-deficient mutant B1020, in the rhizosphere were determined under greenhouse conditions to elucidate the importance of the lipopeptides excreted by these strains in suppressing Foc. Results showed that B. subtilis strain B006 effectively suppressed the disease in natural soil by 42.9%, five weeks after transplanting, whereas B841 and B1020 suppressed the disease by only 22.6% and 7.1%, respectively. Quantitative PCR assays showed that effective colonization of strain B006 in the rhizosphere suppressed Foc propagation by more than 10 times both in nursery substrate and in field-infected soil. Reduction of Foc population at the cucumber stems in a range of $0.96log_{10}ng/g$ to $2.39log_{10}ng/g$ was attained at the third and the fifth weeks of B006 treatment in nursery substrate. In field-infected soil, all three treatments with B. subtilis suppressed Foc infection, indicated by the reduction of Foc population at a range of $2.91log_{10}ng/g$ to $3.36log_{10}ng/g$ at the stem base, one week after transplanting. This study reveals that the suppression of fusarium wilt disease is affected by the effective colonization of the surfactin-producing B. subtilis strain in the rhizosphere. These results improved our understanding of the biocontrol mechanism of the B. subtilis strain B006 in the natural soil and facilitate its application as biocontrol agent in the field.

• 식물병연구
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• 제17권3호
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• pp.295-301
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• 2011

Colletotrichum gloeosporioides에 의해 발생하는 딸기 탄저병에 대한 여러 살균제의 방제효과를 in vitro, 비닐하우스와 노지 육묘포장에서 검정하였다. Benomyl의 $EC_{50}$ 값은 균주 간에 저항성과 감수성 그룹으로 확연히 구별되었다. Iminoctadine tris는 mancozeb나 propineb과 같은 보호살균제보다 낮은 EC50값을 보였으며 mancozeb, propineb, azoxystrobin의 효과는 균주에 따라 다양한 결과를 보였다. 비닐하우스에서, 9종의 살균제를 병원균 접종 전과 후 딸기 '설향' 품종에 처리하였을 때 모든 약제는 무처리에 비해 방제효과가 좋았다. Propineb, mancozeb, azoxystrobin은 병원균 감염 전 처리 시 방제효과가 우수했던 반면 metconazole과 prochloraz-Mn와 같은 ergosterol biosynthesis inhibiting 살균제들은 낮은 탄저병 이병율을 보였다. 2009년과 2010년 노지 육묘포장에서 감소된 살균제 처리 프로그램에 따른 탄저병 방제는 captan 처리 프로그램과 비슷한 방제효과를 보였다. 따라서 prochloraz-Mn 등과 같은 탄저병에 효과적인 살균제를 기반으로 작성된 처리 프로그램은 딸기 농가에 유용하게 사용될 수 있고 탄저병 방제를 위한 더 많은 선택을 제공할 것이다.