• 제목/요약/키워드: Nuclear morphology

검색결과 344건 처리시간 0.023초

Morphology and phylogenetic relationships of two Antarctic strains within the genera Carolibrandtia and Chlorella (Chlorellaceae, Trebouxiophyceae)

  • Hyunsik Chae;Eun Jae Kim;Han Soon Kim;Han-Gu Choi;Sanghee Kim;Ji Hee Kim
    • ALGAE
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    • 제38권4호
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    • pp.241-252
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    • 2023
  • The genera Carolibrandtia and Chlorella have been described as small green algae with spherical cell shapes that inhabit various environments. Species of these genera are often difficult to identify because of their simple morphology and high phenotypic plasticity. We investigated two small coccoid strains from Antarctica based on morphology, molecular phylogeny by two alignment methods which have been applied to previous phylogenetic studies of the genus Chlorella, and comparison of the secondary structures of nuclear small subunit (SSU) and internal transcribed spacer (ITS) rDNA sequences. Light microscopy of two strains revealed spherical cells containing chloroplasts with pyrenoids, and the morphological characteristics of the strains were nearly identical to those of other Chlorella species. However, based on the phylogenetic analyses of nuclear SSU and ITS rDNA sequences, it was determined that the Antarctic microalgal strains belonged to two genera, as the Chlorella and Carolibrandtia. In addition, the secondary structures of the SSU and ITS2 sequences were analyzed to detect compensatory base changes (CBCs) that were used to identify and describe the two strains. A unique CBC in the SSU rDNA gene was decisive for distinguishing strain CCAP 211/45. The ITS2 rDNA sequences for each strain were compared to those obtained previously from other closely related species. Following the comparison of morphological and molecular characteristics, we propose KSF0092 as a new species, Chlorella terrestris sp. nov., and the reassignment of the strain Chlorella antarctica CCAP 211/45 into Carolibrandtia antarctica comb. nov.

INDUCTION PLASMA DEPOSITION TECHNOLOGY FOR NUCLEAR FUEL FABRICATION

  • I. H. Jung;K. K. Bae;Lee, J. W.;Kim, T. K.;M. S. Yang
    • 한국원자력학회:학술대회논문집
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    • 한국원자력학회 1998년도 춘계학술발표회논문집(2)
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    • pp.216-221
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    • 1998
  • A study on induction plasma deposition with ceramic materials, yttria-stabilized-zirconia ZrO$_2$-Y$_2$O$_3$ (m.p 264O $^{\circ}C$), was conducted with a view developing a new method for nuclear fuel fabrication Before making dense pellets more than 96%TD., the spraying condition was optimized through the process parameters, such as chamber pressure, plasma plate power powder spraying distance, sheath gas composition, probe position, particle size and powders different morphology. The results with a 5mm thick deposit on rectangular planar graphite substrates showed a 97.11% theoretical density when the sheath gas flow rate was Ar/H$_2$120/20 l/min, probe position 8cm, particle size -75 ${\mu}{\textrm}{m}$ and spraying distance 22cm by AMDRY146 powder. The degree of influence of the main effects on density were powder morphology. particle size, sheath gas composition, plate power and spraying distance, in that order. Among the two parameter interactions, the sheath gas composition and chamber pressure affects density greatly. By using the multi-pellets mold wheel type, the pellet density did not exceed 94%T.D., owing to the spraying angle.

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Effect of Heat Treatment on Fatigue Crack Growth Rate of Inconel 690

  • Kim, Young-Ho;Lee, Byong-Whi
    • 한국원자력학회:학술대회논문집
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    • 한국원자력학회 1997년도 춘계학술발표회논문집(2)
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    • pp.123-128
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    • 1997
  • The effects of heat treatment on fatigue crack growth rates (FCGRs) of Inconel 690 have been investigated in terms of carbide morphology and grain size. Cycling tests in air at room temperature have shown that FCGR in low stress intensity factor range (ΔK) region can be effectively reduced by increasing the grain boundary carbide precipitate size and grain size. Decrease in FCGR is attributed to the crack tip blunting at the precipitates of grain boundary chromium carbides.

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균열형상변수의 영향 고찰 (A Study on Influences of Crack Morphology Variables)

  • 박원배;이영신
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2004년도 춘계학술대회
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    • pp.324-329
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    • 2004
  • In this study, an application of crack morphology variables in the Leak-Before-Break(LBB) evaluation for nuclear piping systems is investigated, including influences on the leakage crack size and crack instability loads. The crack surface roughness and the number of flow turns as a function of the crack opening displacement are applied to LBB evaluations for KSNP pressurizer surge line, for which fatigue and stress corrosion cracking are considered as failure mechanisms. As a result, there would be a significant impact on safety margins to acceptance criteria for the surge line if crack morphology variables are applied additionally to the current regulatory guide without re-analyses for justification of safety factors being applied on the leakage crack size and piping loads for evaluations.

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자외선 조사처리에 의한 cryptococcus neoformans의 전자현미경적 관찰 (Electron microscopic observations of the irradiation of ultra-violet ray on cryptococcus neoformans)

  • 황동훈;고춘명;최태주;류준
    • 미생물학회지
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    • 제10권1호
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    • pp.29-34
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    • 1972
  • The present study is of ultra-fine structure of Cryptococcus neoformans by means of electron microscopy and reveals the following : 1) In constrast to the bacteria, the normal Cryptococcus neofrmans contains nuclear enveloped with nuclear menbrane, mitochondria, endoplasmic reticulum, distinct cell wall and cell membrane, vacuoles and storage granules as observed in the eucaryotic cells. 2) In apparent cell walls and cell membrane with the appearance of electron transparent area (ETA) and changes of cell morphology were observed in the ultra-violet ray irradiated cell. 2) In apparent cell walls and cell membrance with the appreance of electron transparent area (ETA) and changes of cell morphology were observed in the ultra-violet ray irradiated cell. 3) Morphology changes and cytoplasmic element abnormality was increased with irradiated time. 4) Increase of electron transparent area was thought to be associated with degradation of cell.

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Re-description of Chrysaora pacifica (Goette, 1886) (Cnidaria, Scyphozoa) from Korean Coastal Waters: Morphology and Molecular Comparisons

  • Lee, Hye Eun;Yoon, Won Duk;Chae, Jinho;Ki, Jang-Seu
    • Ocean and Polar Research
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    • 제38권4호
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    • pp.295-301
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    • 2016
  • The nomenclature of the sea nettle jellyfish from Korea was initially described as Dactylometra quinquecirrha Agassiz, 1862. However its identity has been questioned on the basis of its local distribution and molecular data. Here, we examined morphology and DNA sequences of nuclear rDNA using specimens collected from southern Korean waters in August 2014. Based on morphological characteristics (bell size, umbrella pattern, number of tentacles and lappets) and distribution locality, we reassign the Korean D. quinquecirrha to Chrysaora pacifica (Goette, 1886), and provide a re-description accordingly. The molecular identity of C. pacifica was further confirmed by comparison of nuclear ribosomal DNA sequences.