• Food Science and Biotechnology
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• 제16권5호
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• pp.728-733
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• 2007

Bamboo grass, Sasa quelpaertensis, is a native plant to Jeju Island, Korea. The leaves of Sasa plants are widely used in traditional Korean medicine to treat inflammation-related diseases. We investigated the effect of hot water extract from Sasa quelpaertensis leaves (HWE-SQ) on nitric oxide (NO) production and nuclear $factor-{\kappa}B\;(NF-{\kappa}B)$ activation in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. HWE-SQ inhibited LPS-induced NO production and inducible NO synthase (iNOS) protein expression in a dose-dependent manner. Reporter gene assays indicated that HWE-SQ decreases LPS-induced $NF-{\kappa}B$ transcriptional activation. However, HWE-SQ did not affect the phosphorylation and degradation of inhibitory ${\kappa}B{\alpha}\;(1{\kappa}B{\alpha})$. HWE-SQ also directly inhibited iNOS enzyme activity in a dose-dependent manner. These results suggest that HWE-SQ suppresses NO synthesis in macrophages by attenuating $NF-{\kappa}B-mediated$ iNOS protein expression and inhibiting iNOS enzymatic activity, thereby implicating a mechanism by which HWE-SQ is able to ameliorate inflammation-related diseases by limiting excessive or prolonged NO production in pathological events.

• Journal of Radiation Protection and Research
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• 제42권1호
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• pp.1-8
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• 2017

Background: The use of radiation and radioisotopes in Korea has been increasing each year, and its impact on economy and industry is expected to be increasing progressively following the development of industrial technology and the expansion of their usage. To establish and supporting policies for industries using radiation and radioisotopes, it is necessary to check the status of related industries accurately, as well as to gather data required to establish plans for industrial development by studying both revenues and economic scale (contributing to revenue). Materials and Methods: To analyze the status of utilization, surveys were carried out on 6,621 organizations engaged in nuclear operations handling radiation and radioisotopes pursuant to the Nuclear Safety Act as of end 2014, on 33,471 medical institutions using radiation generators for medical and diagnostic purposes pursuant to the Medical Service Act, and on 2,218 organizations using radiation generators for animal diagnostics pursuant to the Veterinary License Act. Results and discussion: The overall status of the domestic radiation market including the number of user organizations, that of employees, and the size of distributions (imports, productions, and exports) with which the scale of domestic radiation market can be judged showed a growth trend compared to the previous year, though the number of employees for radiation operation in industrial sector, research sector, education sector, military sector, and power plants (nuclear power plants) and the size of imports was reduced somewhat. Conclusion: It is expected that data acquired through periodic surveys on the status of utilization would be utilized practically in establishing governmental policies related to the promotion of usage of radiation and radioisotopes, and also be utilized widely in cultivating and developing the industry efficiently to invigorate the related industries.

• Nuclear Engineering and Technology
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• 제52권2호
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• pp.328-336
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• 2020

Cesium is a major product of uranium fission, which is the most commonly existed radionuclide in radioactive wastes. Various technologies have been applied to separate radioactive cesium from radioactive wastes, such as chemical precipitation, solvent extraction, membrane separation and adsorption. Crown ethers and calixarenes derivatives can selectively coordinate with cesium ions by ion-dipole interaction or cation-π interaction, which are promising extractants for cesium ions due to their promising coordinating structure. This review systematically summarized and analyzed the recent advances in the crown ethers and calixarenes derivatives for cesium separation, especially focusing on the adsorbents based on extractants for cesium removal from aqueous solution, such as the grafting coordinating groups (e.g. crown ether and calixarenes) and coordinating polymers (e.g. MOFs) due to their unique coordination ability and selectivity for cesium ions. These adsorbents combined the advantages of extraction and adsorption methods and showed high adsorption capacity for cesium ions, which are promising for cesium separation The key restraints for cesium separation, as well as the newest progress of the adsorbents for cesium separation were also discussed. Finally, some concluding remarks and suggestions for future researches were proposed.

• Asian-Australasian Journal of Animal Sciences
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• 제30권8호
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• pp.1175-1182
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• 2017

Objective: To create genetically modified goat as a biopharming source of recombinant human lacotoferrin (hLF) with transcription activator-like effector nucleases. Methods: TALENs and targeting vector were transferred into cultured fibroblasts to insert hLF cDNA in the goat beta-lactoglobulin (BLG) locus with homology-directed repair. The gene targeted efficiency was checked using sequencing and TE7I assay. The bi-allelic gene targeted colonies were isolated and confirmed with polymerase chain reaction, and used as donor cells for somatic cell nuclear transfer (SCNT). Results: The targeted efficiency for BLG gene was approximately 10%. Among 12 Bi-allelic gene targeted colonies, five were used in first round SCNT and 4 recipients (23%) were confirmed pregnant at 30 d. In second round SCNT, 7 (53%), 4 (31%), and 3 (23%) recipients were confirmed to be pregnant by ultrasound on 30 d, 60 d, and 90 d. Conclusion: This finding signifies the combined use of TALENs and SCNT can generate biallelic knock-in fibroblasts that can be cloned in a fetus. Therefore, it might lay the foundation for transgenic hLF goat generation and possible use of their mammary gland as a bioreactor for large-scale production of recombinant hLF.

• Food Science and Biotechnology
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• 제17권1호
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• pp.188-190
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• 2008

In east Asia, the leaves of various Sasa species have been used in folk medicine for centuries. The effects of the methanolic extract and its subsequent fractions derived from the leaves of Sasa quelpaertensis Nakai on cell proliferation in human leukemia HL-60 cells were evaluated. The ethyl acetate fraction of this extract (ESQL) significantly reduced cell viability in a dose-dependent manner ($0-250\;{\mu}g/mL$). ESQL ($IC_{50}=24.8\;{\mu}g/mL$) exhibited growth inhibition comparable to the main constituent of green tea, epigallocatechin ($IC_{50}=26.2\;{\mu}g/mL$), which was used as a positive control. ESQL treatment induced apoptosis, which was confirmed by the presence of nuclear condensation and annexin V-staining. These results demonstrate that ESQL contains chemopreventive phytochemicals that may be useful in neutraceutical applications.

• 기술혁신연구
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• 제10권1호
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• pp.201-217
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• 2002

Researchers, institutes, and government involved in research & development (R&D) are faced with the problem of performing R&D project evaluations. As a real-word case of evaluation, we elicited and reproduced the evaluation process of the national nuclear R&D projects, using a specific case study of Korea. In the methodology, the analytic hierarchy process (AHP) is employed to evaluate and rank the selected nuclear R&D projects which have a wide range of evaluation objectives and characteristics. As a result, we obtained relative ranking for research projects and importance of evaluation criteria. The method and implications can be easily understood and applied by practitioners since it follows naturally the standard practice of evaluating and relative comparisons of performance using evaluation procedures. By applying particular R&D project output to the procedures, project evaluators can compare and prioritize evaluation objectives.

• 기술혁신학회지
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• 제13권4호
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• pp.794-816
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• 2010

평가위원간 네트워크가 국가연구개발사업의 효율성과 어떠한 영향을 가지는 것인가에 대한 의문을 해소하고자 본 연구는 국가연구개발사업 중 대형사업으로 분류되는 원자력연구개발사업의 2007년 및 2008년간 평가위원의 네트워크를 분석하고 이를 효율성 분석과 연계하여 고찰하여 보았다. 분석을 위해 2개년간 평가에 참여한 평가위원의 기초 자료를 수집하고 효율성 분석을 위해 국가연구개발사업 성과보고서에 제시된 통계치를 토대로 자료포락분석을 활용하였다. 분석 결과 평가위원간 네트워크 정도가 미약할 수록 사업의 효율성이 상대적으로 높게 나타났는데, 이는 사업의 효율성은 연구자가 도출하는 연구 업적도 중요하지만 평가시 섭외되는 평가위원간의 네트워크를 고려하여야 함을 의미한다. 이러한 연구는 그간 평가의원 구성에 있어 전문성 외에도 평가위원간 네트워크를 고려하여야 함을 제시하였다는 점에서 의미를 찾을 수 있다.

• 농업생명과학연구
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• 제50권2호
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• pp.151-160
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• 2016

염증성 사이토카인은 파골세포형성과정에서 중요한 요인이며, 뼈의 흡수는 자주 골다공증과 연결된다. 설포라판은 보로콜리의 화뢰로 부터 분리된 물질로 염증성 사이토카인을 억제한다고 알려져 있다. 본 실험에서는 Receptor activator of nuclear factor kappaB ligand(RANKL)로 자극된 세포에서 설포라판이 파골세포 형성 억제에 대한 효과를 측정하였다. 설포라판은 대식세포인 RAW 264.7 세포에서 파골세포 특이 마커 유전자인 tartrate-resistant acid phosphatase(TRAP), Cathepsin K, matrix metalloproteinase 9(MMP-9), calcitonin receptor을 저해하였으며, TRAP, MMP-9, tumor necrosis factor receptor-associated factor 6(TRAF6)와 전사인자인 nuclease factor of activated T cells(NFATc1)의 단백질 발현과 RANKL로 자극하였을 때 전자인사인 nuclear factor kappaB(NF-kappaB)의 전사활성도 억제 하였다. 이와 같은 결과로 설포라판이 NF-kappaB의 전사활성 억제뿐만 아니라, 파골세포형성인자(TRAP, cathepsin K, MMP-9, calcitonin, NFATc1)와 NFATc1의 발현을 억제시키는 효과가 있음을 확인하였다.

• 기술혁신학회지
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• 제18권1호
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• pp.126-150
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• 2015

본 논문은 한국정부가 유치한 아태원자력협정(RCA)사무국의 국제기구화 추진 현황과 향후 과제를 중심으로 분석하였다. 주요 내용으로는 RCA사무국 유치와 지원활동, 국제법적 지위 확보와 관련된 주요 문제점에 대해서 기술하였다. 이와 함께 RCA 협정개정과 사무국의 국제기구화 추진에 대한 주요 쟁점 분석과 대응 방향을 분석하였다. 한국은 개도국으로서 원자력의 이용개발을 성공적으로 수행한 모델로 평가받고 있으며 앞으로 원자력 기술 선진국으로서 역할을 보여주는 것이 필요하다. 여기에는 국제기구 유치가 적절한 수단이 될 수 있다. 한국정부가 유치에 성공한 RCA사무국의 국제법적 지위 확보는 협정개정이나 보조협정의 체결이 필수적이다. 이를 위해서는 2017년 협정개정과 이후 본부협정 체결을 목표로 범부처 차원의 RCA사무국 국제기구화기획단을 운영하는 것이 바람직이다. 이와 함께 사무국의 지원단도 구성하여 사무국의 사업 수행과 기술적 지원을 수행하는 것도 필요하다.

• 에너지공학
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• 제25권3호
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• pp.136-148
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• 2016

본 논문은 최근 공표된 Post-2020 신기후체제의 국가적 대응에 따른 발전부문의 역할들을 시나리오로 설정하여 경제적 파급효과를 비교 분석 하였다. 이를 위해 제7차 전력수급기본계획에서 제시한 2030년 발전원별 추정 발전량에 IAEA에서 발표한 온실가스 배출계수를 적용하여 온실가스 배출량을 예측하여 발전부문에서 발생시키는 초과 배출량을 산정하였다. 초과 배출량 감축을 위해 세 가지 시나리오를 기반으로 하였으며, 이들은 원자력발전소로 대체, 신재생발전설비 확대, CCS 도입 등이다. 분석 결과 원자력 발전소 대체 시나리오가 배출량 감소 및 경제적인 측면에서 가장 긍정적인 결과를 보였다.