• Journal of Microbiology and Biotechnology
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• v.21 no.5
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• pp.477-482
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• 2011

There are accumulating evidences suggesting that connexin (Cx), a gap junction channel-forming protein, acts as a growth suppressor in various cancer cells, and this effect is attributeed to the gap junction-mediated intercellular communication (GJIC). In order to characterize the relationship between the growth-arresting activity of Cx26 and its cytoplasmic localizations after expression, we linked a nuclear export signal (NES) sequence to Cx26 cDNA before transfecting into a rat breast cancer cell line. A confocal fluorescent microscopic observation revealed that the insertion of NES minimized the nuclear expression of Cx26, and increased its cytoplasmic expression, including plasma membrane junctions. Total cell counting and BrdUrd-labeling experiments showed that the growth of the breast cancer cells was inhibited by 74% upon transfection of Cx26-NES, whereas only 9% inhibition was observed with only Cx26 cDNA.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.71.1-71.1
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• 2003

We show that Cdc6, an essential initiation factor for DNA replication, undergoes caspase-3-mediated cleavage in the early stages of apoptosis in HeLa cells and SK-HEP-1 cells induced by etoposide, paclitaxel, ginsenoside Rh2, or TRAIL. The cleavage occurs at the SEVD$\^$442//G motif and generates an N-terminal truncated Cdc6 fragment (p49-tCdc6) that lacks the carboxy-terminal nuclear export sequence (NES). Cdc6 is known to be phosphorylated by cyclin A-Cyclin A-dependent kinase 2 (Cdk2), an event that promotes its exit from the nucleus and probably blocks it from initiating inappropriate DNA replication. (omitted)

• Korean Journal of Microbiology
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• v.53 no.4
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• pp.292-296
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• 2017

In eukaryote, THO/TREX complex plays a critical role in transcriptional elongation, pre-mRNA processing, and nuclear mRNA export. This complex is evolutionally well- conserved, but there are some differences in composition and function according to organisms. Here we showed that spTex1, a component of THO/TREX complex, is not essential for growth and mRNA export in a fission yeast, Schizosaccharomyces pombe, which is more similar to higher eukaryote than budding yeast. Deletion and overexpression of the spTex1 gene do not lead to any detectable growth phenotype and accumulation of poly(A)+ RNA in the nucleus. And the spTex1-GFP protein is localized mainly in the nucleus. Yeast two-hybrid and Co-immunoprecipitation analysis showed that the spTex1 protein interacted with spHpr1 (THOC1) and spTho2 (THOC2), main subunits of THO complex. We conclude that the S. pombe Tex1 is a component of THO/TREX complex, but does not plays important roles in growth and bulk mRNA export from the nucleus.

• Environmental and Resource Economics Review
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• v.28 no.3
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• pp.385-413
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• 2019

This study examines the determinants of net export of electricity among 30 European countries sharing electricity grid during the period of 1990~2014 by separating the sample period before and after 2005 in which ETS was introduced in Europe. The empirical method used in this study is generalize least squared one considering both heterogeneous and serial correlation in the balanced panel data. According to the empirical results, after 2005 introducing the ETS, holing energy resources, concentrating only on few electricity generation resources, and nuclear electricity generation had played more important role in net export of electricity, while renewable energy had negative effect on net export of electricity and coal and gas generation have no effect on net export after introduction of ETS in Europe probably because of high environmental cost. The policy implication of the results would be that reconsidering each country's optimal generation mix strategy and its role in case freely trading electricity.

• BMB Reports
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• v.49 no.7
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• pp.355-356
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• 2016

The THO/TREX complex consists of several conserved subunits and is required for mRNA export. In metazoans, THO/TREX binds a subset of mRNAs during RNA splicing, and facilitates their nuclear export. How THO/TREX selects RNA targets is, however, incompletely understood. In our recent study, we reported that THO is loaded onto Piwi-interacting RNA (piRNA) precursor transcripts independent of splicing, and facilitates convergent transcription in Drosophila ovary. The precursors are later processed into mature piRNAs, small noncoding RNAs that silence transposable elements (TEs). We observed that piRNAs originating from dual-strand clusters, where precursors are transcribed from both strands, were specifically affected by THO mutation. Analysis of THO-bound RNAs showed enrichment of dual-strand cluster transcripts. Interestingly, THO loading onto piRNA precursors was dependent on Cutoff (Cuff), which comprises the Rhino-Deadlock-Cutoff (RDC) complex that is recruited to dual-strand clusters by recognizing H3K9me3 and licenses convergent transcription from he cluster. We also found that THO mutation affected transcription from dual-strand clusters. Therefore, we concluded that THO/TREX is recruited to dual-strand piRNA clusters, independent of splicing events, via multi-protein interactions with chromatin structure. Then, it facilitates transcription likely by suppressing premature termination to ensure adequate expression of piRNA precursors.

• Journal of the Ergonomics Society of Korea
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• v.33 no.1
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• pp.1-14
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• 2014

Objective: The purpose of this study is to select the methodology for SMR HRA which has characteristics that are different from existing nuclear power plants and digital-based plants. Background: We must assure safety to preoccupy export of technology to developing countries or countries interested in nuclear application. And we can be an advanced country in nuclear technology by securing original technology in the field of SMR such as SMART. Method: THERP, which is the most representative HRA methodology among all, and RARA, which is the latest HRA methodology. This study compared and evaluated THERP and RARA. Results: As a result of applying THERP and RARA methodologies which are based on LOCA EOG task analysis result, this research concluded that RARA has higher personal errors than THERP. Conclusion: This study needs validation for LOCA, emergency operations, normal and abnormal scenarios since HRA methodology was only focused on LOCA scenario. Application: The results of this study can apply as base line data when designing MMIS, which is the main control room of SMART, and when building a simulator.

• KEPCO Journal on Electric Power and Energy
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• v.2 no.1
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• pp.133-141
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• 2016

Although construction of any new nuclear power projects had not been launched since mid-1970s until recently in the USA, many new nuclear power plants have been constructed in many countries with the support of their governments mainly as part of their national energy security and electric source diversification policies. For many reasons, the nuclear power industry seemed to reclaim their renaissance from the beginning of this century and the investment in the nuclear power projects draw positive concern from the private financial sector. But the global financial crisis in 2008 and subsequent economic slow-down together with tighter bank credit regulations caused commercial banks, the main source of financing, to lose appetite for investing in new nuclear power projects. But the nuclear power economics shows that the nuclear power is viable in terms of the environmental benefit and long-term average cost compared to other power generation sources. Also doubt about nuclear power safety was much mitigated due to technology development and reinforced safety-related tests and monitoring. Therefore, the prospect for nuclear power market expansion remains positive although there are comparatively big differences among different scenarios. After Korea Electric Power Corp. won the UAE nuclear power project in December of 2009, the competition in nuclear power markets is undergoing huge changes. Competitors backed by the support of their own governments are now entering the market with many aggressive and innovative financing packages to win bids of new nuclear power projects. This report analyzed the nuclear power market prospects, competitive edges of nuclear power, risk management measures, and financing challenges and recommends alternative solutions to promote competitive edges in winning bids of new nuclear power projects.

• Journal of Fisheries and Marine Sciences Education
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• v.29 no.3
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• pp.920-931
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• 2017

Since the export of Korean-type APR 1400 in 2009 to the UAE, Korea has been achieved management performance, quality inspections, training, nuclear fuel exports for the nuclear power plant. Despite this apparent growth, there are lacking of the research on the marine transportation of radioactive waste. And the terrible accident at the Japan nuclear power plant in 2011 has caused another reconsideration such as emergency response training and plan, reinforcement of safety regulation. According to the Korean government aims to rebuild the appropriate regulation, training, education that is necessary in order to ensure the safety of marine transportation of radioactive waste. Therefore, this study analyzed the various problems identified by the team of experts for the radioactive waste and marine field, the investigation of relevant legal basis, the need for emergency response training for the person in charge of radioactive waste and suggested the simulation-based interactive curriculum during the process of safety verification related to the marine transport of mid- and low-level radioactive waste generated at the Yeon-ggwang nuclear power(Hanbit) plant in 2015.

• Journal of Energy Engineering
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• v.21 no.3
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• pp.292-300
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• 2012

KHNP CRI has been developing APR+ nuclear power plant since 2007, which is GEN III+ model with 4,361 MWth capacity. To develop safer and more economical nuclear power plant than APR1400, we studied domestic and foreign nuclear power plants under construction. We also reviewed nuclear power plants which are appropriate for domestic construction in Korea and also for export. Economic assessments were made twice during the second phase of standard detailed design of the plant. The result of the second phase of economic analysis for APR+ standard detailed design showed that APR+ N-th plant was 24.6% more economical than coal-fired 1,000MW power plant, and was evaluated to be competitive enough in global market for construction of the nuclear power plant.

• Convergence Security Journal
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• v.23 no.4
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• pp.141-151
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• 2023

Since the end of the Cold War in the 1990s, European countries have cut defense costs and reduced armaments as an era of peace without large-scale wars continues, and as a result, the West's defense industry base has gradually weakened. On the other hand, South Korea, the world's only divided country, was able to achieve high growth in the defense industry as a result of continuous arms strengthening in the face of North Korea's nuclear and missile threats. With the rapid increase in demand for conventional weapons systems and changes in the structure of the global defense market due to the Russia-Ukraine war, Korea's weapons system drew great attention as a large-scale defense export contract with Poland was signed in 2022. In 2023, K-Defense ranked ninth in the world's arms exports and aims to become the world's fourth-largest defense exporter by 2027. Therefore, this study analyzed the case of Korea-Poland defense exports to derive problems, and presented development strategies related to export revitalization of K-Defense, a national strategic industry. In order for the defense industry to become Korea's next growth engine, it is necessary to establish a defense organization, prepare government-level measures to protect defense industry technology, and expand military and security cooperation with allies linked to defense exports.