• Nuclear Engineering and Technology
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• 제44권6호
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• pp.597-610
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• 2012

The passive auxiliary feedwater system (PAFS) is one of the advanced safety features adopted in the APR+, which is intended to completely replace the conventional active auxiliary feedwater system. With an aim of validating the cooling and operational performance of PAFS, an experimental program is in progress at KAERI, which is composed of two kinds of tests; the separate effect test and the integral effect test. The separate effect test, PASCAL ($\underline{P}$AF$\underline{S}$ $\underline{C}$ondensing Heat Removal $\underline{A}$ssessment $\underline{L}$oop), is being performed to experimentally investigate the condensation heat transfer and natural convection phenomena in PAFS. A single, nearly-horizontal U-tube, whose dimensions are the same as the prototypic U-tube of the APR+ PAFS, is simulated in the PASCAL test. The PASCAL experimental result showed that the present design of PAFS satisfied the heat removal requirement for cooling down the reactor core during the anticipated accident transients. The integral effect test is in progress to confirm the operational performance of PAFS, coupled with the reactor coolant systems using the ATLAS facility. As the first integral effect test, an FLB (feedwater line break) accident was simulated for the APR+. From the integral effect test result, it could be concluded that the APR+ has the capability of coping with the hypothetical FLB accident by adopting PAFS and proper set-points of its operation.

• Nuclear Engineering and Technology
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• 제23권2호
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• pp.200-209
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• 1991

수칙표면 위에서 일어나는 충류 및 난류응축 모두에 사용할 수 있는 새로운 반경험적 열전달 관계식을 제안하였다. 본 관계식의 함수 형태는 층류와 난류 막응축 유동에 대한 기존의 대표적 관계식에 근거를 두었고, 한편 본 관계식의 수치계수는 공개된 문헌에서 수집한 실험자료를 사용하여 최소자승법에 의해 결정하였다. 또한, 본 관계식과 기존 7개의 관계식 (즉 층류에 대한 관계식 4개 와 난류에 대한 관계식 3개 )의 성능을 정확도와 적용 범위에 대해서 평가하였다. 그 결과 층류 막응축에 대하여는 Zazuli의 관계식과 본 관계식이 가장 작은 평균 오차를 가져오고, 난류 영역에서는 Kirkbride와 Badger의 관계식과 본 관계식이 가장 작은 평균 오차를 가져오는 것을 보여 준다.

• Nuclear Engineering and Technology
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• 제55권8호
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• pp.2812-2822
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• 2023

The supercritical carbon dioxide (sCO₂) Brayton power cycle is more effective than the conventional power cycle and is more widely applicable to heat sources. The inlet working conditions of the compressor have a higher influence on their operating performance because the thermophysical properties of the CO₂ vary dramatically close to the critical point. The flow in the sCO₂ compressor is simulated and the compressor performance is analyzed. The results show that the sCO₂ centrifugal compressor operates outside of its intended parameters due to the change in inlet temperature. The sCO₂ compressor requires more power as the inlet temperature increases. The compressor power is 582 kW when the inlet temperature is at 304 K. But the power is doubled when the inlet temperature increases to 314 K, and the change in the isentropic efficiency is within 5%. The increase in the inlet temperature significantly reduces the risk of condensation in centrifugal compressors. When the heat load of the sCO₂ power system changes, the inlet pressure to the turbine can be kept constant by regulating the rotational speed of compressors. With the increase in rotational speed, the incidence loss and condensation risk increase.

• Reproductive and Developmental Biology
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• 제35권1호
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• pp.105-113
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• 2011

The present study investigated the nuclear remodeling, development potential with telomerase activity and transcription level of X-linked genes (ANT3, HPRT, MeCP2, RPS4X, XIAP, XIST and ZFX) in the bovine somatic cell nuclear transfer (SCNT) embryos using two different fusion and activation methods. Female adult fibroblasts were injected into perivitelline space of in vitro matured oocytes. The oocyte-nucleus complexes were fused and followed by immediately either activated (Group 1), or activated at 1 h post-fusion (hpf) (Group 2), respectively. The incidence of normal premature chromosome condensation (PCC) at 1 hpf was slightly increased in the Group 2, compared to those of Group 1, but there was no significant (p<0.05) difference. The incidence of normal pronucleus (PN) and chromosome spread at 5 and 18 hpf were significantly (p<0.05) higher in the Group 2 than those of Group 1. The cleavage rate to 2-cell stage, developmental rate to blastocyst stage, and the mean number of total and ICM cell numbers were significantly (p<0.05) higher in the Group 2, compared to those of Group 1. Level of telomerase activity was significantly (p<0.05) higher in the SCNT blastocysts of Group 2, compared to those of Group 1. Transcript levels of HPRT, MeCP2 and XIST were not significantly (p<0.05) different between blastocysts of Group 1 and 2. However, transcript level of ANT3, RPS4X, XIAP and ZFX were significantly (p<0.05) up-regulated in the SCNT blastocysts of Group 2, compared to those of Group 1. Taken together, it is concluded that oocyte activation at 1 hpf induces the enhanced developmental potential by efficient nuclear remodeling and subsequent facilitation of the nuclear reprogramming of bovine SCNT embryos.

• 한국어병학회지
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• 제16권1호
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• pp.39-49
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• 2003

Cytosolic oxidation by 4-hydroxy-2-nonenal (4HNE) and tert-butyl hydroperoxide (t-BHP) results in cell death of bovine aortic endothelial cells (BAEC). In this study, we have investigated the roles of antioxidants such as 2,3,6-tribromo-4,5-dihydroxy benzyl methyl ether (TDB) and phloroglucinol in preventing cell death. After treatment with oxidants for 6h, cells became compact and showed nuclear condensation, which were characteristics of early apoptosis. After l2h treatment, morphologic features including severe cytoplasm condensation, membrane blebbing, and apoptotic bodies were prominent and these findings were interpreted as characteristics of late-apoptosis. When the apoptotic cells were treated with antioxidants for 12h, both early and late apoptotic cells did show no significant change. After oxidant treated cells were incubated with antioxidant for 24h, the characteristics of early-apoptosis were eliminated but cells in lateapoptosis could not return to normal cells. These results suggest that TDB and phloroglucinol prevent the cells from dying through apoptosis induced by 4HNE and t-BHP in early stage.

• 한국시뮬레이션학회논문지
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• 제13권2호
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• pp.13-21
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• 2004

The thermal-hydraulic model ARTS which was based on the RETRAN-3D code adopted in the domestic full-scope power plant simulator which was provided in 1998 by KEPRI. Since ARTS is a generalized code to model the components with control volumes, the smaller time-step size should be used even if converged solution could not get in a single volume. Therefore, dedicated models which do not force to reduce the time-step size are sometimes more suitable in terms of a real-time calculation and robustness. In the case of PRT(Pressurizer Relief Tank) model, it is consist of subcooled water in bottom and non-condensable gas in top. The sparger merged under subcooled water enhances condensation. The complicated thermal-hydraulic phenomena such as condensation, phase separation with existence of non-condensable gas makes difficult to simulate. Therefore, the PRT volume can limit the time-step size if we model it with a general control volume. To prevent the time-step size reduction due to convergence failure for simulating this component, we developed a dedicated model for PRT. The dedicated model was expected to provide substantially more accurate predictions in the analysis of the system transients. The results were resonable in terms of accuracy, real-time simulation, robustness and education of operators, complying with the ANSI/ANS-3.5-1998 simulator software performance criteria and RETRAN-3D results.

• 한국분말재료학회지
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• 제14권4호
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• pp.238-244
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• 2007

The electrophoretic deposition process of Ni nano-particles in organic suspension was employed for self-repairing of heat exchanger tubes. For this purpose, Ni nano-particles prepared by levitational gas condensation method were dispersed into the solution of ethanol with the addition of dispersant Hypermer KD2. For electrophoretic deposition of Ni nano-particles on the Ni alloy specimen, constant electric fields of 20 and 100 V $cm^{-1}$ were applied to the specimen in Ni-dispersed solution. It was found that as electrophoretic deposition proceeds, the size of the pit or crack remarkably decreased due to the agglomeration of Ni nano-particles at the pit or crack. This strongly suggests that the electrophoretic mobility of the charged particles is larger for the damaged part with a higher current value rather than outer surfaces with a lower current value.

• Nuclear Engineering and Technology
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• 제49권6호
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• pp.1250-1258
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• 2017

The multigroup transport theory is the basis for many neutronics modules. A significant point of the cross-section (XS) generation procedure is the choice of the energy groups' boundaries in the XS libraries, which must be carefully selected as an unsuitable energy meshing can easily lead to inaccurate results. This decision can require considerable effort and is particularly difficult for the common user, especially if not well-versed in reactor physics. This work investigates a genetic algorithm-based tool which selects an appropriate XS energy structure (ES) specific for the considered problem, to be used for the condensation of a fine multigroup library. The procedure is accelerated by results storage and fitness calculation speedup and can be easily parallelized. The extension is applied to the coupled code SIMMER and tested on the European Sustainable Nuclear Industrial Initiative (ESNII+) Advanced Sodium Technological Reactor for Industrial Demonstration (ASTRID)-like reactor system with different fitness functions. The results show that, when the libraries are condensed based on the ESs suggested by the algorithm, the code actually returns the correct multiplication factor, in both reference and voided conditions. The computational effort reduction obtained by using the condensed library rather than the fine one is assessed and is much higher than the time required for the ES search.

• Applied Microscopy
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• 제27권3호
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• pp.265-279
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• 1997

This study aims to demonstrate the effect of chitosan, one of the natural chelator, on the ultrastructural changes in the mouse liver caused by cadmium. A total of 60 healthy ICR that weighted 30 gm $({\pm}2gm)$ was used for experiment. The experimental group was divided into three groups; group A, B, and C. The group A and B administrated cadmium (4.0 mg/kg) to the intraperitoneal after pretreated with chitosan (0.5% solution) for 30 and 7 days, respectively. Each group was observed at 12, 24, 48, 72 hours and one week after injected cadmium. The results were as follows: 1. Group A The nuclear membrane and the chromatin were normal shapes at overall the time. The inner and outer membranes of the mitochondria damaged a little but almost normal in shapes. And electron-density showed slightly compacted. some enlarged rER (rough endoplasmic reticulum) showed at 12 hours. At 48 hours, typical lamellae of the rER were reformed, and a lot of transvesicles observed around them. To 48 hours, sER (smooth endoplasmic reticulum) was slightly dilated. From 72 hours, sER rehalizated in normal shape. 2. Group B Nuclear membranes were rounded-shape and chromatin showed evenly. To 72 hours, a lot of mitochondria observed around rER and development of cristae showed weakly. But at one week, cristae were clear and electron-density of matrix showed high. At 72 hours lamellae of rER showed some broken, but were reformed at one week. Also at one week, glycogen granules evenly showed over cytoplasm. 3. Group C At 12 hours, Nucleus showed the condensation of nuclear membrane and clear condensation at 24 hours. However, nuclear membrane had a slightly rounded-shape from 72 hours. From 12 hours to the one week, mitochondria showed the dilation of inner cavity and weak development of cristae. Also electron-density of matrix was a little low. Occasionally, destruction of inner and outer membrane observed at one week. The dilation of cisternae and destruction of lamellae of rER showed from 12 to 48 hours. From 72 hours, rER showed slightly dilated only. And lamella observed at one week. In sER, dilation of inner cavity was observed during whole period. These results suggest that chitosan attenuates the toxic effect of the cadmium in the mouse liver.

• 한국수정란이식학회지
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• 제23권1호
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• pp.31-36
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• 2008

We attempted to control the maturation promoting factors (MPF) activity and nuclear remodeling of somatic cell nuclear transfer (NT) bovine embryos. Bovine ear skin fibroblasts were fused to enucleated oocytes treated with either 5 mM caffeine for 2.5 h or 0.5 mM vanadate for 0.5 h and activated. The nuclear remodeling type of the reconstituted embryos was evaluated 1.5 h after activation. MPF activity was assessed in enucleated and chemical treated oocytes before the injection of a donor cell. Effect of chemicals on the embryonic development was evaluated with parthenogenetic embryos. MPF activity increased significantly by caffeine treatment, but decreased by vanadate treatment (p<0.05). Caffeine or vanadate had no deleterious effect on the parthenogenetic embryo development. In caffeine treated group, premature chromosome condensation (PCC) was occurred in 72.2% of NT embryos (p<0.05). In contrast, vanadate induced the formation of a pronucleus-like structure (PN) in a high frequency (68.9%, p<0.05) without PCC (NPCC). Blastocyst development of NT embryos increased by treating with caffeine (30.3%), whereas decreased by treating with vanadate (11.4%) compared to control (22.1%, p<0.05). The results indicate that caffeine or vanadate can control of MPF activity and remodeling type of NT embryos, resulting in the increased or decreased in vitro development.