• 대한인간공학회지
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• 제32권1호
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• pp.77-85
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• 2013

Objective: The aim of this study sets factors from previous research known to impact transfer effects as the independent variables, and examines their relationship with the dependent variables, near transfer effects and far transfer effects. Background: Transfer of Training refers to the application of what learners acquire knowledge and skills in training programs to their job. The ultimate goal of training is to apply what employees learn in training sessions to their workplace. In this sense, transfer of training has been a vital concern for training effectiveness. For training to be effective, trainees(learners) should be able to use what they learn in training program back on the job. Method: For this research purpose, this study conducted a survey on 170 nuclear operators in nuclear education and training center. Of these, survey result from the 167 recruits were sampled. Theoretical model of this study is based on Holton & Baldwin's(2003) distance model of transfer effects. This study sets transfer effects(near transfer, far transfer) as the main dependent variables. Meanwhile, the independent variables are trainee characteristics, training characteristics, organizational transfer climate. Each independent variable has subordinate variables. Subordinate variables of trainee characteristics are self-efficacy, motivation to learn, motivation to transfer and ability to transfer. Subordinate variables of training characteristics are training contents, ability of trainers, training design, training climate. The last Subordinate variables of organizational transfer climate are support of supervisors, support of peer, support of organization. Conclusion: As a analysis result, trainee characteristics appeared to be in effect only significant influence near far transfer of training, the effect of the far transfer of training, there is no significant. In addition, the training characteristics appeared to be having a significant influence on near and far transfer effects. Organizational transfer climate appeared to be having a significant influence on near and far transfer effects. Finally, near transfer effect appeared to be having a significant influence on far transfer effects. Application: Results of this analysis in the study to training organization and training characteristics of the transition environment effects on nuclear power institutions and operators training organization having a significant impact that says. The transfer of knowledge and technology, as well as that can be applied to a new situation in terms of education and training are important characteristics.

• Nuclear Engineering and Technology
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• 제53권8호
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• pp.2464-2476
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• 2021

Direct-contact heat transfer of a single saturated droplet upon colliding with a heated wall in the regime of film boiling was experimentally investigated using high-resolution infrared thermometry technique. This technique provides transient local wall heat flux distributions during the entire collision period. In addition, various physical parameters relevant to the mechanistic modelling of these phenomena can be measured. The obtained results show that when single droplets dynamically collide with a heated surface during film boiling above the Leidenfrost point temperature, typically determined by droplet collision dynamics without considering thermal interactions, small spots of high heat flux due to localized wetting during the collision appear as increasing We_n. A systematic comparison revealed that existing theoretical models do not consider these observed physical phenomena and have lacks in accurately predicting the amount of direct-contact heat transfer. The necessity of developing an improved model to account for the effects of local wetting during the direct-contact heat transfer process is emphasized.

• Reproductive and Developmental Biology
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• 제28권4호
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• pp.241-245
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• 2004

This study was conducted to investigate nuclear remodeling and developmental rate following nuclear transfer of fetal fibroblast cells, ear skin cells and oviduct epithelial cells into porcine recipient oocytes. To test par-thenogenetic activation, oocytes were treated with a 6-dimethylaminopurine (6-DMAP), a single DC-pulse (DC), calcium ionomycin (ionomycin), DC+6-DMAP and ionomycin + 6-DMAP after in vitro maturation. For nuclear transfer, in vitro matured oocytes were enucleated, and donor cells were transferred into oocytes. Cloned embryos were fused and stimulated with 6-DMAP for 4 h and cultured in vitro for 6 days. Among treatments for parthenogenesis, the activation rate of DC +6-DMAP treatment was significantly higher than that of single treatment roups (p<0.01), except for DC treatment group. However, the difference was not significant in activation rate compared to other complex treatment groups. Nuclear swelling of the cloned embryos was initiated at 60 min after stimulation and increased afterwards. Fusion rates were not different among different donor cells. Cleavage rates of DC treatment groups were significantly higher than those of DC+6-DMAP treatment groups (p<0.05) in case that fetal fibroblast and ear cells were used for nuclear donor. The cloned embryos from developed to blastocysts in oviduct epithelial cell nuclear transfer with DC+6-DMAP treatment was significantly higher compared to those with DC only treatment (p<0.05). However, no blastocyst was developed from nuclear transfer of fetal fibroblast and ear cells regardless of activation treatments. Based on these results, a proper activation stimulation may be necessary to increase the activation rate and the development to blastocyst in cloned porcine embryos.

• 한국가축번식학회지
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• 제27권4호
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• pp.349-357
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• 2003

Although nuclear transfer (NT) techniques are used to clone animals, its efficiency is very low. Moreover, nuclear transfer has resulted in offspring with severe developmental problems, probably due to incomplete nuclear reprogramming. Nuclear reprogramming is characterized by functional modification of the transferred nucleus to allow it to direct normal embryo development with the potential to grow to term. Although the nature of the reprogramming factor(s) in mammals is not clear, various nuclear as well as cytoplasmic components are involved in the processes. In this article we review recent data on factors involved in the nuclear reprogramming of cloned embryos.

• Nuclear Engineering and Technology
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• 제41권8호
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• pp.1015-1024
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• 2009

The presence of a non-condensable gas can considerably reduce the level of condensation heat transfer. The non-condensable gas effect is a primary concern in some passive systems used in advanced design concepts, such as the Passive Residual Heat Removal System (PRHRS) of the System-integrated Modular Advanced ReacTor (SMART) and the Passive Containment Cooling System (PCCS) of the Simplified Boiling Water Reactor (SBWR). This study examined the capability of the Multi-dimensional Analysis of Reactor Safety (MARS) code to predict condensation heat transfer in a vertical tube containing a non-condensable gas. Five experiments were simulated to evaluate the MARS code. The results of the simulations showed that the MARS code overestimated the condensation heat transfer coefficient compared to the experimental data. In particular, in small-diameter cases, the MARS predictions showed significant differences from the measured data, and the condensation heat transfer coefficient behavior along the tube did not match the experimental data. A new method for calculating condensation heat transfer coefficient was incorporated in MARS that considers the interfacial shear stress as well as flow condition determination criterion. The predictions were improved by using the new condensation model.

• Journal of Radiation Protection and Research
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• 제28권4호
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• pp.281-290
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• 2003

The KN-12 spent nuclear fuel transport cask, which is a Type B(U) package designed to comply with the requirements of Korea Atomic Energy Act[1], IAEA Safety Standards Series No.TS-R-1[2] and US 10 CFR Part 71[3], is designed for carrying up to 12 PWR spent fuel assemblies in a basket structure. The cask has been licensed in accordance with Korea Atomic Energy Act and was fabricated in Korea in accordance with the requirements of ASME B&PV Sec.III, Div.3[4]. The cask must maintain thermal integrity in accordance with the related regulations and be evaluated to verify that the thermal performance of the cask complies with the regulatory requirements. The temperatures of the cask and components were determined by using finite elements methods with a numerical tool, safety tests using an 1/8 height slice model of the real cask were conducted to demonstrate verification of the numerical tool and methods, and heat transfer tests for normal transport conditions were performed as a fabrication acceptance test to demonstrate the heat transfer capability of the cask.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.7-7
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• 2001

Cryopreservation by vitrification of Hanwoo blastocysts derived from nuclear transfer with Hanwoo adult ear cell was compared with that of in vitro fertilized blastocysts. For vitrification, day 7 or day 8 blastocysts were serially exposed in glycerol (G) or/and ethylene glycol (EG) mixtures (10% G for 5 min, 10% G plus 20% EG for 5 min, and 25% G plus 25% EG for 30 sec) which was diluted in 10% FBS added D-PBS. Thawing of straw was carried out in air for 10 sec and then in water bath of $25^{\circ}C$ for 20 sec. The contents of the straw (0.2 ml) was expelled into a culture dish contained with 0.5M sucrose and 10% FBS(S-DPBS) by cutting the cotton plug and then the recovered embryos were put into fresh 0.25M and 0.125M S-DPBS for 30 sec, respectively, The embryos were transferred into D-PBS with 10% FBS for 5 min. and were cultured in a 10u1 droplet of co-culture environment (cumulus cell monolayer + 10% added CRl medium) for 24 h. In the result, survival rates were 88.9% and 85.4% for nuclear transfer embryos and in vitro fertilized embryos, respectively. After transfer of vitrified-thawed blastocysts produced from nuclear transfer, 4 of 5 total recipients did not return to the subsequent estrus cycle at 30 days. It is concluded that the Hanwoo blastocysts derived from nuclear transfer can be successfully cryopreserved using simple and efficient vitrification method.

• Nuclear Engineering and Technology
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• 제28권6호
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• pp.532-541
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• 1996

Theoretical and numerical study on heat transfer and evaporation in the vertical channel has been carried out and basic correlations have been derived for the heat transfer evaluation of PCCS. Analysis program was developed with low-Reynolds-number k-$\varepsilon$ model and surface transfer rates were calculated for the turbulent natural convection in the vertical channel. In relation to dry cooling by buoyancy-driven air, first, the system parameters which govern overall heat transfer rate are determined through the adequate nondimensionalization procedure. After comparison with existing experimental data, numerical results are used to derive heat transfer correlation by sensitivity calculations. In relation to wet cooling by falling water film, numerical analysis are carried out for evaporation process with real film surface conditions and evaporation correlation is derived through analogy concept and correction factors.

• Nuclear Engineering and Technology
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• 제52권4호
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• pp.708-720
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• 2020

Core-wide temperature distribution in sodium-cooled fast reactor plays a key role in its decay heat removal process, however the prediction for temperature distribution is quite complex due to the conjugate heat transfer between the assembly flow and the inter-wrapper flow. Hybrid medium model has been proposed for conjugate heat transfer modeling in the core. The core is modeled with a Realistic modeled inter-wrapper flow and hybrid medium modeled assembly flow. To validate present model, simulations for a three-assembly model were performed with Realistic modeling, traditional porous medium model and hybrid medium model, respectively. The influences of Uniform/Non-Uniform power distribution among assemblies and the Peclet number within the assembly flow have been considered. Compared to traditional porous medium model, present model shows a better agreement with in Realistic modeling prediction of the temperature distribution and the radial heat transfer between the inter-wrapper flow and the assembly flow.

• 농업과학연구
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• 제33권1호
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• pp.35-41
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• 2006

이종간의 핵이식은 확보가 쉬운 난자를 이용함으로서 용이한 수핵란의 확보와 윤리적인 문제등을 피할 수 있으므로 매우 유용한 방법이다. 본 연구에서는 이러한 이종간 핵이식의 조건을 규명하고자 유산양 태아섬유아세포를 이용하여 돼지의 난자에 이종간 핵이식을 시도하였다. 돼지와 산양의 난소에서 난포란을 채취하여 각각 NCSU-23, TCM-199에 호르몬을 첨가한 성숙배양액에 배양하여 $38^{\circ}C$, 5% $CO_2$의 배양기에서 48시간 동안 체외성숙 시켜 수핵난자를 준비하였다. 공여세포는 유산양의 태아섬유아세포는 DMEM배양액에서 배양한 후 0.25% Trypsin-EDTA 용액으로 처리하여 single-cell로 분리하여 사용하였다. 돼지와 산양의 성숙란에 공여세포를 핵치환하여 0.3 M mannitol fusion medium에 넣어 각각 DC 1.2 kV/cm $30{\mu}sec$과 DC 2.39 kV/cm $15{\mu}sec$의 조건으로 BTX를 이용 2회의 전기충격으로 융합 활성화하였다. 활성화된 돼지와 산양 핵이식란은 각각 PZM-3와 mSOF 배양액에 7일간 배양하면서 할구분열율과 배발달율을 관찰하여 동종간 핵이식란과 비교하였다. 비교결과 이종간 핵이식란의 경우 분열률이 58.9%, 배반포기로의 발달률이 5.4%로 돼지 동종간 핵이식란의 분열율이 67.4%, 배반포기로의 배발달율은 13.6% 보다 낮게 나타났고, 또한 산양의 동종간 핵이식란배아의 분열율 81%, 상실배와 배반포기로의 발달률이 54.7% 보다 낮게 나타났다. 이러한 결과로 이종간의 이식은 많은 잇점에도 불구하고 아직은 낮은 배발달률을 보이고 있어 이에 대한 핵이식 조건 및 체외배양 조건 등 많은 부분에서의 추가적인 연구가 필요한 것으로 사료된다.