• Title/Summary/Keyword: Novel techniques

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Imaging Single-mRNA Localization and Translation in Live Neurons

  • Lee, Byung Hun;Bae, Seong-Woo;Shim, Jaeyoun Jay;Park, Sung Young;Park, Hye Yoon
    • Molecules and Cells
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    • v.39 no.12
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    • pp.841-846
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    • 2016
  • Local protein synthesis mediates precise spatio-temporal regulation of gene expression for neuronal functions such as long-term plasticity, axon guidance and regeneration. To reveal the underlying mechanisms of local translation, it is crucial to understand mRNA transport, localization and translation in live neurons. Among various techniques for mRNA analysis, fluorescence microscopy has been widely used as the most direct method to study localization of mRNA. Live-cell imaging of single RNA molecules is particularly advantageous to dissect the highly heterogeneous and dynamic nature of messenger ribonucleoprotein (mRNP) complexes in neurons. Here, we review recent advances in the study of mRNA localization and translation in live neurons using novel techniques for single-RNA imaging.

High capacity multi-bit data hiding based on modified histogram shifting technique

  • Sivasubramanian, Nandhini;Konganathan, Gunaseelan;Rao, Yeragudipati Venkata Ramana
    • ETRI Journal
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    • v.40 no.5
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    • pp.677-686
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    • 2018
  • A novel data hiding technique based on modified histogram shifting that incorporates multi-bit secret data hiding is proposed. The proposed technique divides the image pixel values into embeddable and nonembeddable pixel values. Embeddable pixel values are those that are within a specified limit interval surrounding the peak value of an image. The limit interval is calculated from the number of secret bits to be embedded into each embeddable pixel value. The embedded secret bits can be perfectly extracted from the stego image at the receiver side without any overhead bits. From the simulation, it is found that the proposed technique produces a better quality stego image compared to other data hiding techniques, for the same embedding rate. Since the proposed technique only embeds the secret bits in a limited number of pixel values, the change in the visual quality of the stego image is negligible when compared to other data hiding techniques.

Minimally Invasive Repair Technique of Achilles Tendon Using Sponge Forceps: A Technical Report (스폰지 겸자를 이용한 아킬레스건의 최소 절개 봉합술: 술기 보고)

  • Park, Sam Guk;Park, Chul Hyun
    • Journal of Korean Foot and Ankle Society
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    • v.20 no.2
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    • pp.88-91
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    • 2016
  • Various minimally invasive repair techniques have been performed for acute Achilles tendon rupture. Despite this, it is difficult to use these techniques in common practice because of the necessity of special instruments. We propose a novel minimal invasive technique using sponge holding forceps, which are commonly used in the operating room for the acute Achilles tendon rupture.

A Technique for Removing Adjacent Induction Noise Mixed with Partial Discharge Signals of High Voltage Rotating Machines (고압 회전기 부분방전 신호에 혼합된 인접상 유도 잡음 제거 기법)

  • Youn, Young-Woo;Yi, Sang-Hwa;Hwang, Don-Ha;Choo, Young-Bae;Kang, Dong-Sik
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.58 no.2
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    • pp.335-341
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    • 2009
  • Analysis of the partial discharge signal, a technique to diagnose the stator winding insulation is a key function for the diagnosis of high voltage rotating machines and requires high precision. To satisfy this requirement, various denoising techniques such as filtering and differential methods were proposed. However, these techniques can not eliminate a adjacent induction noise that decreases reliability of the diagnosis. A simple novel denoising algorithm, therefore, is proposed for removing the adjacent induction noise in this paper. The algorithm shows good performance in the real partial discharge signals measured by 13kV class capacitive couplers installed at hydro-generator in Dae-cheong Dam.

Novel Techniques for Real Time Computing Critical Clearing Time SIME-B and CCS-B

  • Dinh, Hung Nguyen;Nguyen, Minh Y.;Yoon, Yong Tae
    • Journal of Electrical Engineering and Technology
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    • v.8 no.2
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    • pp.197-205
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    • 2013
  • Real time transient stability assessment mainly depends on real-time prediction. Unfortunately, conventional techniques based on offline analysis are too slow and unreliable in complex power systems. Hence, fast and reliable stability prediction methods and simple stability criterions must be developed for real time purposes. In this paper, two new methods for real time determining critical clearing time based on clustering identification are proposed. This article is covering three main sections: (i) clustering generators and recognizing critical group; (ii) replacing the multi-machine system by a two-machine dynamic equivalent and eventually, to a one-machine-infinite-bus system; (iii) presenting a new method to predict post-fault trajectory and two simple algorithms for calculating critical clearing time, respectively established upon two different transient stability criterions. The performance is expected to figure out critical clearing time within 100ms-150ms and with an acceptable accuracy.

Development of a meshless finite mixture (MFM) method

  • Cheng, J.Q.;Lee, H.P.;Li, Hua
    • Structural Engineering and Mechanics
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    • v.17 no.5
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    • pp.671-690
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    • 2004
  • A meshless method with novel variation of point collocation by finite mixture approximation is developed in this paper, termed the meshless finite mixture (MFM) method. It is based on the finite mixture theorem and consists of two or more existing meshless techniques for exploitation of their respective merits for the numerical solution of partial differential boundary value (PDBV) problems. In this representation, the classical reproducing kernel particle and differential quadrature techniques are mixed in a point collocation framework. The least-square method is used to optimize the value of the weight coefficient to construct the final finite mixture approximation with higher accuracy and numerical stability. In order to validate the developed MFM method, several one- and two-dimensional PDBV problems are studied with different mixed boundary conditions. From the numerical results, it is observed that the optimized MFM weight coefficient can improve significantly the numerical stability and accuracy of the newly developed MFM method for the various PDBV problems.

A Novel Tensile Specimen and Test Machine for Mechanical Properties of MEMS Materials (MEMS 소재의 기계적 특성 평가를 위한 인장형 시편 및 시험기 제작)

  • Park, Jun-Hyub;Kim, Chung-Youb;Lee, Chang-Seun;Choa, Sung-Hoon;Song, Ji-Ho
    • Proceedings of the KSME Conference
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    • 2004.04a
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    • pp.258-263
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    • 2004
  • Mechanical property evaluation of micrometer-sized structures is necessary to help design reliable microelectromechanical systems(MEMS) devices. Most material properties are known to exhibit dependence on specimen size and such properties of microscale structures are not well characterized. This paper describes techniques developed for tensile testing of materials used in MEMS. Epi-polycrystalline silicon is currently the most widely used material, and its tensile strength has been measured as 1.52GPa. We have developed an uniaxial testing machine for testing microscale specimen using electro-magnetic actuator. The field magnet and the moving coil taken from an audio-speaker were utilized as the components of the actuator. Structure of specimen was designed and manufactured for easy handling and alignment. In addition to the static tensile tests, new techniques and procedures for measuring strength are described.

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A Novel Tensile Specimen and Tensile Tester for Mechanical Properties of Thin Films (박막의 기계적 물성을 위한 새로운 인장 시편 및 인장 시험기)

  • Park, Jun-Hyub;Kim, Yun-Jae
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.31 no.6 s.261
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    • pp.644-650
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    • 2007
  • Mechanical property evaluation of micrometer-sized structures is necessary to help design reliable microelectromechanical systems(MEMS) devices. Most material properties are known to exhibit dependence on specimen size and such properties of microscale structures are not well characterized. This paper describes techniques developed for tensile testing of thin film used in MEMS. Epi-polycrystalline silicon is currently the most widely used material, and its tensile strength has been measured as 1.52GPa. We have developed a tensile testing machine for testing microscale specimen using electro-magnetic actuator. The field magnet and the moving coil taken from an audio-speaker were utilized as the components of the actuator. Structure of specimen was designed and manufactured for easy handling and alignment. In addition to the static tensile tests, it is described that new techniques and procedures can be adopted for high cycle fatigue test of a thin film.

Analysis of Single-RF MIMO Receiver with Beam-Switching Antenna

  • Gwak, Donghyuk;Sohn, Illsoo;Lee, Seung Hwan
    • ETRI Journal
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    • v.37 no.4
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    • pp.647-656
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    • 2015
  • This paper proposes a single-RF MIMO receiver that adopts a beam-switching antenna (BSA) instead of a conventional array antenna. The beauty of the proposed single-RF MIMO receiver with BSA is that it can be deployed in a very small physical space while achieving a full spatial multiplexing gain. Our analysis has revealed that the use of a BSA inevitably results in the spectrum spreading effect at the RF output, which in turn causes an SNR decrease and adjacent channel interference (ACI). Two novel receiver techniques are proposed to mitigate the issues of redundant sub-band suppression and ACI avoidance. Numerical analysis results verify the performance improvement from the proposed receiver techniques.

A Secure WLAN Authentication Scheme

  • Singh, Rajeev;Sharma, Teek Parval
    • IEIE Transactions on Smart Processing and Computing
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    • v.2 no.3
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    • pp.176-187
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    • 2013
  • Message replay, malicious Access Point (AP) associations and Denial of Service (DoS) attacks are the major threats in Wireless LANs. These threats are possible due to a lack of proper authentication and insecure message communications between wireless devices. Current wireless authentication & key exchange (AKE) schemes and security protocols (WEP, WPA and IEEE 802.11i) are not sufficient against these threats. This paper presents a novel Secure WLAN Authentication Scheme (SWAS). The scheme introduces the delegation concept of mobile authentication in WLANs, and provides mutual authentication to all parties (Wireless Station, Access Point and Authentication Server). The messages involved in the process serve both authentication and key refreshing purposes. The scheme enhances the security by protecting the messages through cryptographic techniques and reduces the DoS impact. The results showed that cryptographic techniques do not result in extra latencies in authentication. The scheme also reduces the communication cost and network overhead.

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