• Weed & Turfgrass Science
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• 제4권2호
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• pp.118-123
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• 2015

메타게놈(metagenome)은 연구실에서 배양이 불가능한 미생물을 포함한 자연계에 존재하는 미생물의 유전자를 직접 연구하는 학문분야이다. 지구상 거의 모든 자연, 인공 환경에서 살고 있는 미생물 DNA를 분리 정제하는 것이 가능하며, 재조합 DNA 기술 등을 이용하여 배양 가능한 숙주미생물에 메타지놈을 클로닝함으로서 메타지놈 라이브러리를 제작할 수 있다. 최근 메타게노믹스를 통하여 자연계에 존재하고 있는 대다수의 미생물들이 실험실에서 배양되지 않았던 이유를 구명할 수 있게 되었고, 그들이 가지고 있는 생태학적인 의미와 역할에 대한 이해와 더불어 점차 그 응용 분야와 범위도 확대되고 있다. 이와 같은 메타게놈의 응용 분야 확대의 한 방안으로 본 연구에서는 새로운 제초활성 물질 및 제초활성 물질 생산에 필요한 유전자를 확보하기 위해 메타게놈 라이브러리를 대상으로 오이 떡잎절편 검정, 미세조류 생장저해 검정, 종자발아 저해 검정의 HTS (high throughput screening) 시스템을 구축하였고, 구축된 시스템으로부터 선발된 최종 단일 클론인 9-G1과 9-G12의 바랭이에 대한 in vivo assay를 통해 본 연구에서 개발한 HTS 시스템의 유효성을 확인 하였다. 후속연구로서 활성단일클론이 만들어내는 제초활성물질의 동정 및 제초활성물질을 합성하는 유전자군에 대한 연구를 수행 중에 있다.

• Journal of Microbiology and Biotechnology
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• 제11권4호
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• pp.656-662
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• 2001

Angiogenesis is known as a crucial process in the growth and spreading of tumor cells. Accordingly, the effective inhibition of this process would appear to be a promising way to cure angiogenesis-related diseases, including cancer. This study demonstrates that acalycixenolide E (AX-E) from the marine organism Acalycigorgia inermis exhibits a potent anti-angiogenic activity both in vitro and in vivo. AX-E inhibits the bFGF-induced proliferation of HUVECs in a dose dependent manner, along with the bFGF-induced migration, invasion, and tube formation of HUVECs. Moreover, AX-E potently inhibits the in vivo neovascularization of the chorioallantoic membranes (CAMs) of growing chick embryos. interestingly, AX-E suppresses the expression of metalloproteases 2 and 9, yet shows no effect on their activities. The novel chemical structure and potent anti-angiogenic activity of AX-E will be of great value in elucidating the molecular mechanism of angiogenesis as well as in the development of a novel anti-angiogenic drug.

• 대한화학회지
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• 제48권1호
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• pp.39-45
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• 2004

산에 민감한 작용기를 갖는 tert-butyl methacrylate(tBMA)와 산 증식 기능을 갖는 4-hydroxy-4''p-styrenesulfonyloxyisopropylidene dicyclohexane(HSI)과 4-p-styrenesulfonyloxy-4''-tosyloxyisopropylidene dicyclohexane (STI)를 둘 다 함께 갖고 있는 공중합체를 새로운 고분자 산증식형 포토레지스트로 합성하였다. 산증식형 공중합체인 Poly(HSI-co-tBMA) film과 Poly(STI-co-tBMA) film은 산의 부재 시에는 레지스트 공정 온도에 대하여 충분한 열적 안정성을 나타내었다. Poly(STI-co-tBMA) film의 감도는 tBMA homopolymer에 비교하여 2배 정도 증진되었지만, Poly(HSI-co-tBMA) film은 오히려 2배 정도 감도가 저하되는 결과를 나타내었다. 고분자에 도입한 이러한 산증식 기능을 갖는 그룹의 구조에 따라 광감도 증진 효과가 다르게 나타남을 확인하였다.

• Bulletin of the Korean Chemical Society
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• 제32권5호
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• pp.1635-1639
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• 2011

An efficient and novel positive charge-doping on the sidewalls of multi-walled carbon nanotubes has been achieved in the presence of tetrahydrofuran as a dopant and Lewis acidic ionic liquids, [bmim]$Sb_nF_{5n+1}$ (n ${\geq}$ 2; bmim = 1-butyl-3-methylimidazolium), as an activator, leaving air-stable derivatives having positively charged sidewalls and the counter anions, [MWCNT$^{y+}$][SbF$_6^-$]$_y$ (MWCNT = multi-walled carbon nanotube). The derivatization took place very fast in one-pot and under mild reaction conditions. The ionic structure enabled a tunable dissolution of the derivatives in various solvents through anion exchange.

• Bulletin of the Korean Chemical Society
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• 제33권9호
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• pp.2930-2936
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• 2012

A three-dimensional pharmacophore hypothesis was developed for atypical antipsychotics in order to map common structural features of highly active compounds by using HipHop in CATALYST program. The pharmacophore hypotheses were generated using 12 compounds as training set and validated using 11 compounds as test set. The most predictive hypothesis (Hypo1) comprises five features viz. two hydrophobic regions, two hydrogen bond acceptor lipid and one aromatic ring. In the absence of information like crystallized structure of 5-$HT_{2A}$ receptor and binding mode of antipsychotics with 5-$HT_{2A}$ receptor, this hypothesis will serve as a potentially valuable tool in the design of novel atypical antipsychotics acting primarily at 5-$HT_{2A}$ and $D_2$ receptors.

• Fibers and Polymers
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• 제5권3호
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• pp.219-224
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• 2004

The reactive printing properties of regular viscose rayon and a new regenerated cellulosic fiber (en Vix^{\textregistered}$) which was prepared from cellulose acetate fiber was investigated in a comparative manner. From the results, it was found that en Vix exhibited better printing properties than regular viscose rayon. It showed stable final color yields, irrespective of the amount of thickener, hence reproducibility of printing of en Vix is expected to be excellent. In addition, urea requirements were less for the printings on en Vix than for the corresponding printing on viscose rayon. Therefore, en Vix is also expected to reduce the amount of the urea which causes environmental problems in dyehouse effluent.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2005년도 International Meeting on Information Displayvol.II
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• pp.1119-1122
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• 2005

A selective deposition of Co thin films for thin film transistor gate electrode has been carried out by the growth with combination of micro-contact printing and metal organic chemical vapor deposition (MOCVD). This results in the elimination of optical lithography process. MOCVD has been employed to selectively deposit Co films on preformed OTS gate pattern by using micro-contact printing (${\mu}CP$). A hydrogenated amorphous silicon TFT with a Co gate selectively formed on SAMs patterned structure exhibited a subthreshold slope of 0.88V/dec, and mobility of $0.35cm^2/V-s$, on/off current ratio of $10^6$, and a threshold voltage of 2.5V, and thus demonstrating the successful application of the novel bottom-up approach into the fabrication of a-Si:H TFTs.

• Bulletin of the Korean Chemical Society
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• 제27권8호
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• pp.1206-1210
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• 2006

Four isostructural amino-acid-based polyoxomolybdates, {$M(H_2O)_3(pro)Mo_4O_{13}$}$_2{\cdot}2H_2O$ (pro = proline, M = Co (1), Ni (2), Cu (3), Zn (4)), have been synthesized and structurally characterized by single crystal X-ray diffraction, elemental analysis, IR spectrum, TG analysis. The structures of 1-4 are layered networks built up from {$Mo_8O_{26}(pro)_2$}$^{4-}$ units and {$M(H_2O)_3O_3$} octahedra, the uncoordinated water molecules occupying the interlayer regions.

• Bulletin of the Korean Chemical Society
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• 제32권2호
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• pp.620-624
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• 2011

In recent years, the study of self-assembly peptide used in drug delivery system has been attracted great interest from scientists. In the category are self-assembly peptides in the structure either with one hydrophobic surface and another hydrophilic or a hydrophobic head and a hydrophilic tail. Here, we focus on a novel designed peptide EYK with double amphiphilic surfaces, investigating on the capability of peptide as a carrier for hydrophobic compound model pyrene. The fluorescence data presented the dynamic process of the transfer, showing that the pyrene was in the crystalline form in peptide solution, and molecularly migrated from its peptide encapsulations into the membrane bilayers when the peptide-pyrene suspension was mixed with liposome vesicles. The results indicated that the peptide EYK could stabilize hydrophobic pyrene in aqueous solution and delivered it into EPC liposome as a potential carrier.

• Bulletin of the Korean Chemical Society
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• 제32권2호
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• pp.498-502
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• 2011

The divergent synthesis of novel phosphorus-containing dendrimer with 4,4'-sulphonyldiphenol at the core has been accomplished involving simple condensation reactions using $P(O)Cl_3$, $P(S)Cl_3$, 3-amino-phenol, 3-hydroxy-benzaldehyde, and 2-butyn 1, 4-diol. The final compound was a Schiff's base macromolecule possessing 4 imine bonds, 8 acetylenic bonds and 8 OH groups at the periphery. The structures of intermediate compounds were confirmed by IR, NMR ($^1H$, $^{13}C$ and $^{31}P$), LC-Mass and C, H, N analysis. The structure of the final dendrimer (5) was confirmed by IR, NMR ($^1H$, $^{13}C$ and $^{31}P$), MALDI-TOF-MS, and C, H, N analysis. The surface morphological characteristics of the final dendrimer were understood by Scanning Electronic Microscopic study (SEM). The thermal stability of the final dendrimer was studied by TGA/DTA analysis.