• Journal of Korean Neurosurgical Society
• /
• v.61 no.4
• /
• pp.441-449
• /
• 2018

Objective : Notch receptors are heterodimeric transmembrane proteins that regulate cell fate, such as differentiation, proliferation, and apoptosis. Dysregulated Notch pathway signaling has been observed in glioblastomas, as well as in other human malignancies. Nerve growth factor (NGF) is essential for cell growth and differentiation in the nervous system. Recent reports suggest that NGF stimulates glioblastoma proliferation. However, the relationship between NGF and Notch1 in glioblastomas remains unknown. Therefore, we investigated expression of Notch1 in a glioblastoma cell line (U87-MG), and examined the relationship between NGF and Notch1 signaling. Methods : We evaluated expression of Notch1 in human glioblastomas and normal brain tissues by immunohistochemical staining. The effect of NGF on glioblastoma cell line (U87-MG) was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. To evaluate the relationship between NGF and Notch1 signaling, Notch1 and Hes1 expression were evaluated by reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. To confirm the effects of NGF on Notch1 signaling, Notch1 and Hes1 small interfering RNAs (siRNAs) were used. Results : In immunohistochemistry, Notch1 expression was higher in glioblastoma than in normal brain tissue. MTT assay showed that NGF stimulates U87-MG cells in a dose-dependent manner. RT-PCR and Western blot analysis demonstrated that Notch1 and Hes1 expression were increased by NGF in a dose-dependent manner. After transfection with Notch1 and Hes1 siRNAs, there was no significant difference between controls and 100 nM $NGF-{\beta}$, which means that U87-MG cell proliferation was suppressed by Notch1 and Hes1 siRNAs. Conclusion : These results indicate that NGF stimulates glioblastoma cell proliferation via Notch1 signaling through Hes 1.

• Journal of the Korean Wood Science and Technology
• /
• v.26 no.4
• /
• pp.6-12
• /
• 1998

Test results of domestic softwood lumber were presented to examine the notch effect of beams and compare to present AIJ(Architecture Institute of Japan) formula in notched wood member especially positioned in bottom side (tension side) of a beam. Notched lumber was tested under following condition : each specimen supported simply, and subjected to third-point loading at points of 1/3 of the span length. Notch was located opposite side to loading direction and notch depth were 1/6, 1/4, 1/3 of beam depth. Deflection and load were measured by digital dial guage each in 25kgf increment. Bending test results were as follows; Mpro/Mmax range (proportional and maxium bending moment ratio in notched beam) was 0.5 - 0.65. It was considered that maxium bending moment was about 1.5 times to proportional bending moment in notched beam and showed same tendency in the test result of ordinary wood specimens. AU standard formula for the tension side notch, Mmat = 0.6 ${\times}$ (Zo $\sigma$), the constant 0.6 was suitble for notch ratio(notch depth to beam depth) 1/6, but this ratio for 1/4, and 1/3 was not. So it is preferable to accept smaller value than 0.6 for notch ratio more than 1/3. These experiment results showed critical effect in tension side notched wood beam especially in greater than notch ratio 1.3 of wood beam. From the above results, it is recommened to revise design formula adoptable to domestic wood constructon member with tension side notched member.

• Archives of Plastic Surgery
• /
• v.37 no.1
• /
• pp.7-11
• /
• 2010

Purpose: Vascular endothelial growth factor (VEGF) is known as a growth factor of endothelium and fibroblast. The purpose is to know the VEGF effects on fibroblast proliferation and fibroblast's notch receptor expression. Methods: CCD-986sk fibroblast was purchased from the Korean Cell Bank and was used in XTT assay for proliferation and wound healing assay for migration. Immunofluorescent (IF) staining and western blotting were used in testing notch expression of fibroblast. Semiquantitative RT-PCR was used in checking notch 1 mRNA production by fibroblast. Student-t test was used for analyzing results. Results: Cell proliferation assay using XTT showed significant higher proliferation in VEGF treated fibroblast, $2.324{\pm}0.0026$ vs. $2.463{\pm}0.017$ (p=0.002). Wound healing assay showed longer migration in VEGF treated fibroblast (p=0.062). The fluorescence was brighter in VEGF treated cells of notch 1 IF staining. Notch 1 expressions and mRNA productions increased more in VEGF treated cells. Conclusion: VEGF stimulates fibroblast to proliferate, migrate and to express Notch 1 simultaneously. Notch receptor could be related to VEGF mediated wound healing.

• Structural Engineering and Mechanics
• /
• v.69 no.1
• /
• pp.51-67
• /
• 2019

In this paper, the anisotropy of tensile behaviours of layered rocks consisting internal notch has been investigated using particle flow code. For this purpose, firstly calibration of PFC2D was performed using Brazilian tensile strength. Secondly Brazilian test models consisting bedding layer was simulated numerically. Thickness of layers was 10 mm and layered angularity was $90^{\circ}$, $75^{\circ}$, $60^{\circ}$, $45^{\circ}$, $30^{\circ}$, $15^{\circ}$ and $0^{\circ}$. The strength of bedding interface was too high. Each model was consisted of one internal notch. Notch length is 1 cm, 2 cm and 4 cm and notch angularities are $60^{\circ}$, $45^{\circ}$, $30^{\circ}$, $15^{\circ}$ and $0^{\circ}$. Totally, 90 model were tested. The results show that failure pattern was affected by notch orientation and notch length. It's to be noted that layer angle has not any effect on the failure pattern. Also, Brazilian tensile strength is affected by notch orientation and notch length.

• Journal of the Korea Concrete Institute
• /
• v.24 no.5
• /
• pp.525-533
• /
• 2012

In this study, bending tests were performed on beam specimens made of UHPCC with the fiber content range of 0~5 vol% to investigate the contribution of fiber content to first cracking strength and flexural tensile strength. Also, four-point bending tests for unnotched beam as well as three-point bending test for notched beam were performed to estimate the effect of the presence of notch on the strengths. The experiment result showed that the increase in fiber content made linear improvement in the flexural tensile strength; whereas first cracking strength was enhanced only when at least 1 vol% of fibers was incorporated. Comparison of the bending test results with and without notch showed that the notch effect varied with the fiber content. The increase in fiber content diminished the effect of stress concentration on the notch tip, reducing the difference in the strengths. With much higher fiber content, the effect of stress concentration almost disappeared and the defection on cracking plane or the size effect dominated the strengths, consequently resulting in higher strengths in the notched beams than the unnotched ones.

• Journal of Microbiology
• /
• v.44 no.2
• /
• pp.217-225
• /
• 2006

Kaposi's sarcoma-associated herpesvirus (KSHV) RTA transcription factor is recruited to its responsive elements through interaction with RBP-Jk that is a downstream transcription factor of the Notch signaling pathway that is important in development and cell fate determination. This suggests that KSHV RTA mimics cellular Notch signal transduction to activate viral lytic gene expression. Here, I demonstrated that unlike other B lymphoma cells, KSHV -infected primary effusion lymphoma BCBL1 cells displayed the constitutive activation of ligand-mediated Notch signal transduction, evidenced by the Jagged ligand expression and the complete proteolytic process of Notch receptor I. In order to investigate the effect of Notch signal transduction on KSHV gene expression, human Notch intracellular (hNIC) domain that constitutively activates RBP-Jk transcription factor activity was expressed in BCBL1 cells, TRExBCBL1-hNIC, in a tetracycline inducible manner. Gene expression profiling showed that like RTA, hNIC robustly induced expression of a number of viral genes including KS immune modulatory gene resulting in downregulation of MHC I and CD54 surface expression. Finally, the genetic analysis of KSHV genome demonstrated that the hNIC-mediated expression of KS during viral latency consequently conferred the downregulation of MHC I and CD54 surface expression. These results indicate that cellular. Notch signal transduction provides a novel expression profiling of KSHV immune deregulatory gene that consequently confers the escape of host immune surveillance during viral latency.

• Proceedings of the KIEE Conference
• /
• 2009.07a
• /
• pp.893_894
• /
• 2009

This paper study on the notch effect for d-q axis inductance of interior type permanent magnet (IPM) motor. The variations of d-q axis inductance are analyzed by finite element method (FEM). Applying the notch for cogging torque minimization and maximization of reluctance torque is confirmed to be improve the torque characteristics. Also, speed characteristics is analyzed according to the load current

• Proceedings of the KWS Conference
• /
• 2002.10a
• /
• pp.145-150
• /
• 2002

It has been well known that ductile fracture of steels is accelerated by triaxial stresses. The characteristics of ductile crack initiation in steels are evaluated quantitatively using two-parameters criterion based on equivalent plastic strain and stress triaxiality. It has been demonstrated by authors using round-bar specimens with circumferential notch in single tension that the critical strain to initiate ductile crack from specimen center depends considerably on stress triaxiality, but surface cracking of notch root is in accordance with constant strain condition. In order to evaluate the stress/strain state in the specimens, especially under dynamic loading, a thermal, elastic-plastic, dynamic finite element (FE) analysis considering the temperature rise due to plastic deformation has been carried out. This study provides the fundamental clarification of the effect of strength mismatching, which can elevate plastic constraint due to heterogeneous plastic straining, loading mode and loading rate on critical condition to initiate ductile crack from notch root using equivalent plastic strain and stress triaxiality based on the two-parameter criterion obtained on homogeneous specimens under static tension. The critical condition to initiate ductile crack from notch root for strength mismatched bend specimens under both static and dynamic loading would be almost the same as that for homogeneous tensile specimens with circumferential sharp notch under static loading.

• Journal of Welding and Joining
• /
• v.16 no.4
• /
• pp.73-82
• /
• 1998

This paper is especially concerned with the weldment between support beam and square bar that plays important roles in control box of Expansion Joint as a brdige structure. Fatigue strength ({TEX}$$\sigma$_{ult}${/TEX}) of the weldment is dependent on notch factor ({TEX}$K_{f}${/TEX}) become important factors to predict fatigue life. The fatigue notch sensitivity (η) for metals can be divided into two types ; high and low notch sensitivity. In this work, the Expansion Joint weldment was found to have low notch sensitivity. The maximum strain distribution during static loading is similar to the FEM analysis. Fatigue test of real structure was performed up to {TEX}$10^{6}${/TEX} cycles to be compared with predicted endurance limit.

• Computers and Concrete
• /
• v.22 no.1
• /
• pp.39-51
• /
• 2018

This study introduces a new algorithm to determine size independent values of fracture energy, fracture toughness, and fracture process zone length in three-point bending specimens with shallow to deep notches. By using the exact beam theory, a concept of equivalent notch length is introduced for specimens with no notches in order to predict the peak loads with acceptable precisions. Moreover, the method considers the variations of fracture process zone length and effects of higher order terms of stress field in each specimen size. In this paper, it was demonstrated that the use of some recently developed size effect laws raises some concerns due to the use of nonlinear regression analysis. By using a comprehensive fracture test data, provided by Hoover and Bazant, the algorithm has been assessed. It could be concluded that the proposed algorithm can facilitate a powerful tool for size effect study of three-point bending specimens with different notch lengths.