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Function and Oligomerization Study of the Leucine Zipper-like Domain in P13 from Leucania separata Multiple Nuclear Polyhedrosis Virus

  • Du, Enqi;Yao, Lunguang;Xu, Hua;Lu, Songya;Qi, Yipeng
    • BMB Reports
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    • v.40 no.2
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    • pp.232-238
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    • 2007
  • The p13 gene is uniquely present in Group II nucleopolyhedroviruses (NPVs) and some granuloviruses, but not in Group I NPVs. p13 gene was first described by our laboratory in Leucania separatamultiple nuclear polyhedrosis virus (Ls-p13) in 1995. However, the functions of Ls-P13 and of its homologues are unknown. When Ls-p13 was inserted into Autographa californica nucleopolyhedrovirus, a Group I NPV, polyhedra yield was inhibited. However, this inhibition was prevented when the leucine zipper-like domain of Ls-p13 was mutated. To determine the cause of this marked difference between Ls-P13 and leucine zipper mutated Ls-P13 (Ls-P13mL), oligomerization and secondary structure analyses were performed. High performance liquid chromatography and yeast two-hybrid assays indicated that neither Ls-P13 nor Ls-P13mL could form oligomers. Informatics and circular dichroism spectropolarimetry results further indicated marked secondary structural differences between Ls-P13 and Ls-P13mL. The LZLD of Ls-P13 has two extended heptad repeat units which form a hydrophobic surface, but it is short of a third hydrophobic heptad repeat unit for oligomerization. However, the mutated LZLD of Ls-P13mL lacks the above hydrophobic surface, and its secondary structure is markedly different. This difference in its secondary structure may explain why Ls-P13mL is unable to inhibit polyhedra yield.

Improved analytical method for adhesive stresses in plated beam: Effect of shear deformation

  • Guenaneche, B.;Benyoucef, S.;Tounsi, A.;Adda Bedia, E.A.
    • Advances in concrete construction
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    • v.7 no.3
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    • pp.151-166
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    • 2019
  • This paper introduces a new efficient analytical method, based on shear deformations obtained with 2D elasticity theory approach, to perform an explicit closed-form solution for calculation the interfacial shear and normal stresses in plated RC beam. The materials of plate, necessary for the reinforcement of the beam, are in general made with fiber reinforced polymers (Carbon or Glass) or steel. The experimental tests showed that at the ends of the plate, high shear and normal stresses are developed, consequently a debonding phenomenon at this position produce a sudden failure of the soffit plate. The interfacial stresses play a significant role in understanding this premature debonding failure of such repaired structures. In order to efficiently model the calculation of the interfacial stresses we have integrated the effect of shear deformations using the equilibrium equations of the elasticity. The approach of this method includes stress-strain and strain-displacement relationships for the adhesive and adherends. The use of the stresses continuity conditions at interfaces between the adhesive and adherents, results pair of second-order and fourth-order coupled ordinary differential equations. The analytical solution for this coupled differential equations give new explicit closed-form solution including shear deformations effects. This new solution is indented for applications of all plated beam. Finally, numerical results obtained with this method are in agreement of the existing solutions and the experimental results.

A Study on the Analysis of Concrete Vertical form Demolding Timing Considering the Relationship between the Type of Coarse Aggregate and Ultrasonic Pulse Velocity (굵은 골재의 종류와 초음파 속도의 관계성을 고려한 콘크리트 수직 거푸집 해체 시점 분석에 관한 연구)

  • Nam, Young-Jin;Kim, Won-Chang;Choi, Hyeong-Gil;Lee, Tae-Gyu
    • Journal of the Korea Institute of Building Construction
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    • v.23 no.6
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    • pp.683-692
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    • 2023
  • This research assesses the mechanical properties of concrete, utilizing both normal and lightweight aggregates, through measurements of compressive strength and ultrasonic pulse velocity. The study observed that concrete with normal aggregates exhibited higher compressive strength in its initial stages, whereas concrete with lightweight aggregates showed increased strength over time, likely attributed to the higher water absorption rate of lightweight aggregates. Ultrasonic pulse velocity generally registered higher in normal aggregate concrete, barring a specific duration, presumably due to variations in the internal pore structure of the aggregates. The correlation coefficient(R2) for the strength prediction equation, derived from the relationship between compressive strength and ultrasonic pulse velocity, exceeds 0.95. This high correlation suggests that the predictive equation based on these experimental findings is a reliable method for estimating concrete strength.

A Morpheme-unit Korean Feature-Based Brammer (KFG) with the X-bar Theoretic Notion of Headedness (X-바 이론의 중심어 개념을 도입한 형태소 단위의 한국어 자질 기반 문법)

  • Park, So-Yeong;Hwang, Yeong-Suk;Im, Hae-Chang
    • Journal of KIISE:Software and Applications
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    • v.26 no.10
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    • pp.1247-1259
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    • 1999
  • 본 논문에서는 한국어 문장형성원리를 간결하게 제시할 수 있도록 X-바 이론의 중심어 개념을 도입한 한국어 자질기반 문법을 제안한다. 제안하는 문법은 어절에 관계없이 나타나는 한국어의 문법현상을 명확히 설명할 수 있도록 어절 대신 형태소를 기본단위로 한다. 그리고, 한국어의 구문범주가 지닌 의미정보와 기능정보를 자질을 이용하여 독립적으로 표현하며, 구문범주간의 결합관계를 바탕으로 하는 자질연산을 수행하여 문장을 분석한다. 또한, 한국어의 부분자유어순과 생략현상에 대해 견고하게 분석할 수 있도록 자질연산을 이진결합중심의 CNF(Chomsky Normal Form)로 제한한다. 이렇게 구성된 한국어 자질기반 문법은 규칙을 직관적이고도 간단하게 기술하며, 한국어의 다양한 문장들을 견고하게 분석한다. SERI Test Suites 97과 신문기사에서 746문장을 추출하여 실험한 결과 94%~99%의 적용율을 보였다.Abstract In this paper, we propose a Korean feature-based grammar(KFG) which adopts the X-bar theoretic notion of headedness for a precise representation of Korean syntactic structure. In order to explain various language phenomena in a given sentence, we use not the word but the morpheme as a constituent unit of KFG. We use features manifesting both the syntactic information and the semantic information of Korean syntactic categories, and feature operations based on the association relationship between two categories. In addition, we restrict feature operations to CNF(Chomsky Normal Form) binary form, which provides a robust representation for properties in Korean such as the frequent ellipsis and the partial free-order. The KFG is intuitive, simple, and versatile in representing most Korean sentences. The experimental result shows 94%~99% coverage on 746 sentences extracted from SERI Test Suites 97 and newspaper sentences.

Study on the Manoeuvring Characteristics of a Ship with Stern Bulb (선미벌브를 갖는 선박의 조종특성에 관한 연구)

  • Kyoung-Ho Sohn;Gyoung-Woo Lee
    • Journal of the Society of Naval Architects of Korea
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    • v.31 no.3
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    • pp.65-79
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    • 1994
  • In the present paper, An emphasis is laid upon effects of stern bulb on hydrodynamic property and manoeuvring performance. We carried out captive model tests in circulating water channel with two ship models of which the frame lines of aft bodies are different. such as normal stern form and stern form with bulb, but of which the other parts are exactly same. The tests conducted consist of hull resistance test, effective thrust measurement, oblique tow test, and measurements of factors related to rudder force. From the results of model tests, we discussed effects of stern bulb on hull forces and on hull-propeller-rudder interactions, comparing with normal stern form. Furthermore, we also discussed effects of stern bulb on course stability. turning ability. spiral characteristics and zig-zag manoeuvre by computer simulation. As a result, it is clarified that the adoption of stern bulb makes course stability the worse and turning ability the better. The difference of the hydrodynamic derivatives of naked hull between two ship forms cause the worse course stability of the ship with stern bulb. The differences of the effective inflow velocity to rudder and hull forces induced by steered rudder cause the better turning ability of the ship with stern bulb.

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Development of a Custom-Made Dress Form for Draping Based on 3D Handheld Scanners and 3D Printing Technology (3D 핸디형 스캐너와 3D 프린팅 기술 기반 드레이핑용 커스텀 메이드 드레스폼 개발)

  • Ryu, Eun Joo;Song, Hwa Kyung
    • Fashion & Textile Research Journal
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    • v.24 no.4
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    • pp.451-459
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    • 2022
  • This study aimed to develop a a custom-made dress form for draping using a live model's 3D body scan obtained from an entry-level 3D handheld scanners, 3D modeling software and 3D printing technology. A female subject was recruited whose body size fell under the normal (N) body shape criteria suggested by KS K 0051. First, the handheld scanner reduced the length of the legs in scanning, but most of the scanning operations between the neck and crotch levels were conducted accurately. Therefore, this study was designed to develop a torso dress form. The full body 3D scan was edited into a torso shape using ZBrush® software. Using Rhinoceros® and Materialise's Magics software, a 3D body scan was modeled so that the user could fit two types of mannequin stands (one with a neck fixation from above and one with an insert from below) to the dress form. The body scan was divided into 9 pieces to fit the printable size of the Stratasys 3D printer Fortus 250mc, and the cross-sectional distance from the center to the periphery was downsized by 2 mm. After outputting the dress form scan file with a 3D printer, the dress form was manufactured by the first covering it with a 4 oz nonwoven pad and the second covering with a single jersey material.

The Effects of Panax Ginseng Radix Pharmacopuncture and Zizyphi Spinosi Semen Pharmacopuncture on the Heart Rate Variability (인삼약침과 산조인약침이 정상인의 심박 변이도에 미치는 영향)

  • Seol, Hyun;Song, Beom-Yong;Yook, Tae-Han
    • Journal of Acupuncture Research
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    • v.26 no.5
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    • pp.19-28
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    • 2009
  • Objectives : We tried to investigate the effects of Panax Ginseng Radix pharmacopuncture and Zizyphi Spinosi Semen pharmacopuncture on human body with the Heart Rate Variability (HRV) in adult man. As well as we tried to investigate how safe Panax Ginseng Radix and Zizyphi Spinosi Semen pharmacopuncture on the the human being. Methods : We investigated on 44 healthy volunteers consisted of 16 subjects in Panax Ginseng Radix pharmacopuncture group, 13 subjects in Zizyphi Spinosi Semen pharmacopuncture group and 15 subjects in Normal Saline. We ruled out subjects who wasn't stable by rest, with in the limit of normal heart beat(60-120cycle/min). Study form was a randomized, clinical trial. Each group was injected Panax Ginseng Radix pharmacopuncture, Zizyphi Spinosi Semen pharmacopuncture and Normal Saline $0.2m{\ell}$ at $GB_{21}$(Kyonjong). We measured HRV on 7 times : before and after injection per 5 minutes during 30 minutes. The SPSS 13.0 for windows was used to analyze the data by the paired t-test(in the group) and Anova test(among the groups) were used to verify the result. Results : 1. After Panax Ginseng Radix pharmacopuncture injection, SDNN was significantly high, pNN50 was significantly low, Ln(TP) was significantly high, Ln(VLF) was significantly high, Ln(LF) was significantly high at 30 minutes. 2. After Zizyphi Spinosi Semen pharmacopuncture injection, Complexity was significantly low, Ln(VLF) was significantly high at 30 minutes. 3. HRV-index made a significant difference between the Zizyphi Spinosi Semen pharmacopuncture and normal saline at 5 minutes(p=0.02). 4. There wasn's any pain the injected region and whole body, rubefaction, uredo, nausea, vomiting, colic, headache, dizziness within 24 hours. Measured data and mean value of Mean-HRV was within normal range. Conclusions : The results suggested that and Panax Ginseng Radix pharmacopuncture in healthy adult man tended to activate the vagus nervous system within normal range.

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The Functionality of Facial Appearance and Its Importance to a Korean Population

  • Kim, Young Jun;Park, Jang Wan;Kim, Jeong Min;Park, Sun Hyung;Hwang, Jae Ha;Kim, Kwang Seog;Lee, Sam Yong;Shin, Jun Ho
    • Archives of Plastic Surgery
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    • v.40 no.6
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    • pp.715-720
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    • 2013
  • Background Many people have an interest in the correction of facial scars or deformities caused by trauma. The increasing ability to correct such flaws has been one of the reasons for the increase in the popularity of facial plastic surgery. In addition to its roles in communication, breathing, eating, olfaction and vision, the appearance of the face also plays an important role in human interactions, including during social activities. However, studies on the importance of the functional role of facial appearance. As a function of the face are scare. Therefore, in the present study, we evaluated the importance of the functions of the face in Korea. Methods We conducted an online panel survey of 300 participants (age range, 20-70 years). Each respondent was administered the demographic data form, Facial Function Assessment Scale, Rosenberg Self-Esteem Scale, and standard gamble questionnaires. Results In the evaluation on the importance of facial functions, a normal appearance was considered as important as communication, breathing, speech, and vision. Of the 300 participants, 85% stated that a normal appearance is important in social activities. Conclusions The results of this survey involving a cross-section of the Korean population indicated that a normal appearance was considered one of the principal facial functions. A normal appearance was considered more important than the functions of olfaction and expression. Moreover, a normal appearance was determined to be an important facial function for leading a normal life in Korea.

Study on the Comparison of Emotion between the Mothers with Low-birth Weights and Normal Infants and the Effect of Home Visiting for the Low-birth Weights (저체중출생아 어머니와 정상신생아 어머니의 정서와 지지 비교 및 보건소 저체중출생아 가정방문간호의 효과에 대한 연구)

  • Bang, Kyung-Sook;Kim, Yong-Soon;Park, Jee-Won
    • Korean Parent-Child Health Journal
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    • v.5 no.1
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    • pp.75-89
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    • 2002
  • This study was conducted to compare the emotional state between the mothers with low-birth-weights and mothers with normal infants, and to analyze the effects of home visiting for the low-birth-weights in one city. Data were collected from 51 mothers with low-birth-weights and 90 mothers with normal infants to compare emotional state, and from 26 mothers with low-birth weights to evaluate the effect of home visiting care. Summaries of results were as follows; 1. In mothers with low-birth-weights, social support form others was significantly lower than those of mothers with normal infants. Although the differences were not significant, mothers with low-birth-weights have more stress and child rearing burden, and less maternal self-esteem than those of mothers with normal infants. 2. Mothers with low-birth-weights, the more burden, postpartum depression, and the less husbands' support they felt. When they had lower maternal self-esteem and lower husbands' support, child rearing burden was higher. Also there was significant negative correlation between maternal self-esteem and postpartum depression. 3. In mothers with low-birth-weights, the score of post-intervention stress, care-giving burden, and postpartum depression were somewhat decreased, and maternal self-esteem was increased than pre-intervention data, although they were not statistically significant. 4. Mothers' satisfaction on the home-visiting care was considered to be high. In summary, mothers with low-birth-weights had lower social support even though they experienced more stress than mothers with normal infants. Therefore, public health nurse in community should pay more attention to them.

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Volumetric analysis of normal condyles and those with disc displacement with reduction in the Indonesian population: A CBCT study

  • Nawawi, Azkya Patria;Rikmasari, Rasmi;Kurnikasari, Erna;Oscandar, Fahmi;Lita, Yurika Ambar
    • Imaging Science in Dentistry
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    • v.52 no.1
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    • pp.103-108
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    • 2022
  • Purpose: Disc displacement can cause resorption of the head of the condyle and affect its volume. This study analysed the volume of normal condyles and those with disc displacement with reduction (DDR) in cone-beam computed tomography (CBCT) scans from the Indonesian population. Materials and Methods: This study analysed 56 condyles (26 normal and 30 with DDR) from patients who visited the Oral and Maxillofacial Radiology Unit after being referred from the Prosthodontics Unit at Dental Hospital Universitas Padjadjaran from December 2020 to February 2021. Samples were divided into 2 groups (normal and DDR left and right-side condyles) based on the DC/TMD Axis 1 form through the clinical examination results. Both sample groups were exposed to CBCT radiation. The CBCT imaging results in the Digital Imaging and Communications in Medicine format were exported to the open-source ITK-SNAP format to determine condyle volume. Volumetric data from the cortical and trabecular areas of the right or left side condyles were arranged by sex. The independent t-test was used to determine the significance of differences with IBM SPSS version 21.0. Intra- and inter-observer reliability and validity were tested before determining the volume of the condyles. Results: Normal condyles and DDR condyles showed significant differences in volume (P<0.05). Significant differences were also seen in cortical (P=0.0007) and trabecular (P=0.0045) volumes. There was a significant difference in condylar volume based on sex. Conclusion: The normal condyle volume was significantly different from the DDR condyle volume in both sexes.