• Asian-Australasian Journal of Animal Sciences
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• 제20권5호
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• pp.693-700
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• 2007

The present study was conducted to examine the effect of different levels of essential and nonessential amino acid in NCSU-23 medium on the in vitro-produced porcine embryo as it develops from the zygote to the blastocyst stage. Four experiments were performed, each with a completely randomized design involving 5 to 8 replications of treatments. In order to know the effect of nonessential amino acids in NCSU-23 medium, 0, 5, 10 and $20{\mu}/ml$ MEM were supplemented there to, (Exp. 1) and the medium was supplemented with same level of essential amino acids (Exp. 2). The combined effect of nonessential (0, 5, 10 and $20{\mu}/ml$ MEM) and essential amino acids (0, 5, 10 and $10{\mu}/ml$ MEM) in NCSU-23 medium (Exp. 3), first 72 h with non-essential amino acids (at 0, 5, 10 and $20{\mu}/ml$ MEM), and last 4 d with essential amino acids with the same level as NEAA (Exp. 4) were examined. The embryo development was monitored and the quality of blastocysts was evaluated by counting the number of total cells and determining the ratio of inner cell mass (ICM) to trophoectoderm (TE) cells. When Eagle's nonessential amino acids (MEM) added to NCSU-23 medium, it significantly increased the likelihood of development to the 2- to 4-cell stage and subsequent blastocyst development. Supplementation of different levels of essential amino acids in the NCSU-23 medium decreased cleavage rate, rate of morula and blastocyst development and the number of ICMs. In the case of the combined effect of essential and nonessential amino acids, better and significant results were found for blastocysts, hatching blastocysts and for ICM numbers which were also dose dependent. With respect to the biphasic effect of nonessential and essential amino acids, nonessential amino acids increased cleavage whereas essential amino acids increased the total cell number. Neither the nonessential nor the essential group of amino acids, on their own, affected blastocyst cell number or the differentiation of cells in the blastocyst. In conclusion, this study determined the role of nonessential and essential amino acids in the culture of the porcine embryo and showed that the embryo requires different levels of amino acids as it develops from the zygote to the blastocyst stage.

• 품질경영학회지
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• 제19권2호
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• pp.117-124
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• 1991

In this paper, it is assumed that a system is composed of an essential unit and a nonessential unit. During the running of the system, an essential unit is replaced at periodic replacement time T or at nth failure of essential unit whichever occurs first. Nonessential unit is replaced at its failure and at the replacement of essential unit. This paper derive optimal replacement period which minmises the total expected cost for replacement. The unimodality of totoal maintenance cost function is proved under the assumption that hazard rate of each component is continuous and monotone increasing failure rate(IFR). Based on this condition, it is shown that the optimal replacement period is finite and unique.

• Journal of Nutrition and Health
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• 제5권4호
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• pp.145-150
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• 1972

In order to establish a new biochemical parameter to evaluate nuitrtitional status. the present paper presents a paper chromatographic procedure. which enables clear separation of the essential and nonessential free amino acids in the urine without desalting the sample prior to chromatographic analysis The children of school age. showing difference in their body physiques. from the rural and urban areas in Korea were selected in collecting the 24 hour urine specimens. with the following conclusions. (1) A new procedure utilyzing paper chromatographic analysis. without desalting the urine. was proposed to establish a new biochemical parameter for nutritional status by analyzing the ratio of the nonessential to the essential free amino acids in urine. (2) According to the pesent procedure. the ratio obtained with the urban children was proved to be $1.33{\pm}0.18$, whereas that with the rural children was $1.59{\pm}0.24$; suggesting the lower the ratio. the superior the body physiques and nutritional status. (3) The ratio had a statistically significant correlation with the body and sitting heights as well as the body weight. but not with the girth.

• 한국식품영양과학회지
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• 제13권4호
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• pp.421-426
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• 1984

20-39세 사이의 성인 남자 34명과 여자 35명에 대하여 혈청 유리 아미노산의 정상치를 구하였다. 성인 남녀 다 같이 threonine(일부glutamine이 포함)alanine, lysine이 아미노산 중에서 약 40%를 차지하고 있었으며, 총 필수 아미노산의 함량은 총비필수 아미노산의 함량보다 유의하게 낮았다. 혈청아미노산 조성에서 뚜렷한 성별의 차이가 나타났는데 성인 남자에서는 alanine, cystine, valine, isoleucine, leucine, tyrosine, tryptophan, threonine, methionine, proline 등이 , 여자에서는 lysine, histidine이 각각 유의하게 높은 수준을 보였다. 총 필수 아미노산의 함량 그리고 총비필수 아미노산 및 총 아미노산에 대한 총필수 아미노산의 몰비도 성인 남자가 유의성 있는 높은 수준을 나타내었다. 아울러 총필수 아미노산에 대한 각 필수 아미노산의 몰비를 구하여 성별로 비교하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제9권3호
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• pp.241-245
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• 1996

A chronological account of the prospect and problems of utilization of urea in poultry diets is presented. Urea has long been considered as toxic to poultry but recent research, although limited, has yielded controversial results. The main problem appears to be due to the fact that whether or not response to urea is dependent on environment (germ free versus conventional). Although caecum is found to be the major site of ammonia production from urea, the so called nutritional benefit derived by chicken fed urea is probably limited to its utilization for the synthesis of nonessential amino acids in the protein depleted chicken, but not in the protein adequate chicken. More research is needed to monitor production characteristics of birds fed urea and investigate its toxic effect, if any, in some greater detail before recommending this nonprotein nitrogenous substance for inclusion in the poultry diets.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.272-274
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• 2000

Temperate bacteriophage P2 has a nonessential gene called old(overcoming lysoginization defection). In the presence of old, the growth of the host (Escherichia coli) with recBC- genotype is ingibited, and another bacteriophage, lambda, cannot superinfect. The Old protein has been shown to possess an exonuclease actibity. Three mutant P2s(old 1, old 17, old 49) which did gene was coned into expression vectors to produce hexahistidine-tagged proteins. The proteins were affinity-purified and shown to lose its exonuclease activity on both double-stranded and single-stranded DNA substrates. Thus, it was concluded that the lambda exclusion was related to Old's exonuclease activity.

• Asian-Australasian Journal of Animal Sciences
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• 제22권2호
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• pp.296-304
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• 2009

The nutritional significance of essential amino acids, as well as non-essential amino acids, is well documented in poultry production with regards to growth performance and protein accretion. However, the function of amino acids in the stress response is still unclear. L-Pipecolic acid, a L-lysine metabolite in the brain, induced a hypnotic and sedative effect acting via the ${\gamma}$- aminobutyric acid receptors. L-Arginine also induced a sedative effect via its metabolism to L-ornithine. In addition, three-carbon nonessential amino acids like L-alanine, L-serine and L-cysteine also induced sedative effects. These facts suggest that the requirement for amino acids in both essential and non-essential types may require reconsideration to add the concept of stress amelioration in the future.

• 보건행정학회지
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• 제3권1호
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• pp.102-123
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• 1993

This study investigates effcts of HMO internal structural arrangements on performance, specially cost reduction measured by hospitalization rate. This study formulates formalization, centralization measured by decision-making participation, differentiation, and coordination as structural factors, considering coordination as an intermediate factor between the rest of structural factors and hospitalization rate. The commonly used HMO types is assumed not effective in explaining performance differences. For the empirical test, I use bootstrap regression analyses with 48 HMOs. The results of the analyses show that HMO types fail to explain differences in hospitalization rate. However, dicision-making participation and differention effectively reduce hospiatalization rate, while frmalization increases hospitalization rate and coordination has nonessential effect on hospitalization rate. And, formalization and decision-making participation positively contribute to achieve coordination in HMO. These findings suggest that the theoretical framework derived from rational-citingency theory of formal organization better explains performance differences of HMOs than HMO types.

• 한국가축번식학회지
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• 제18권4호
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• pp.235-244
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• 1995

생쥐 배반포기 내부세포괴를 체외에서 분리 배양하여 분화가 억제된 내부세포괴 유래 증식세포를 미분화 상태에서 무한히 증식할 수 있는 전능성을 지닌 배아간세포(embryonic stem cell : ES cell)로 확립하고자 본 연구를 실시하였다. BCF1 생쥐 배반포를 10% FCS, 0.1mM nonessential amino acid, 0.1mM sodium pyruvate, 0.1mM 2-mercaptoethanol과 1,000U/ml LIF(세포분확억제인자)가 첨가된 DMEM 기초배양액에 mitomycin-C를 처리한 STO 단층배양세포에서 배양하여 분화가 억제된 내부세포괴 유래의 배아간세포를 분리하였다. 배반포를 STO 단층배양세포에서 4일간 배양하여 내부세포괴세포를 신선한 STO 단층배양세포에서 약 5일 간격으로 반복하여 계대배양을 실시하였다. 5차 계대배양후 뚜렷한 분화 양상없이 배양된 미분화 세포군에 대한 alkaline phosphatase (AP)염색과 체외분화능 검색을 실시한 결과 적색의 미분화 AP 양성반응이 확인되었으며 체외에서 배분화 형성이 유도됨에 따라 배양된 배아간세포주의 다능성 배아간세포 특성을 확인할 수 있었다.

• Fisheries and Aquatic Sciences
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• 제9권2호
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• pp.49-56
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• 2006

We evaluated changes in the nutritional composition of Mideodeok Styela clava harvested during the months of January, March, and May 2005. Glutamic acid, aspartic acid, arginine, leucine, and lysine were the most dominant amino acids present. Ratios of essential amino acids to nonessential amino acids were comparable to those of many fish species, with values ranging from 0.55 to 0.61 and 0.66 to 0.67 for muscle of Mideodeok from Geoje and Tongyeong, respectively. Mideodeok seems to be a suitable source of important fatty acids as it contains high levels of polyunsaturated fatty acids. Eicosapentanoic acid EPA; 20:5n-3 and docosahexanoic acid DHA; 22:6n-3 were the most dominant fatty acids, ranging from 20.0 to 22.3% and 16.5 to 17.9% in muscle, and 20.3 to 23.2% and 15.2 to 18.8% in tunic, respectively. The total mineral fraction of Mideodeok was 22.2-27.3% of dry matter. Sodium, calcium, magnesium, and potassium were the most dominant minerals in both muscle and tunic.