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Filtering-Based Method and Hardware Architecture for Drivable Area Detection in Road Environment Including Vegetation (초목을 포함한 도로 환경에서 주행 가능 영역 검출을 위한 필터링 기반 방법 및 하드웨어 구조)

  • Kim, Younghyeon;Ha, Jiseok;Choi, Cheol-Ho;Moon, Byungin
    • KIPS Transactions on Software and Data Engineering
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    • v.11 no.1
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    • pp.51-58
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    • 2022
  • Drivable area detection, one of the main functions of advanced driver assistance systems, means detecting an area where a vehicle can safely drive. The drivable area detection is closely related to the safety of the driver and it requires high accuracy with real-time operation. To satisfy these conditions, V-disparity-based method is widely used to detect a drivable area by calculating the road disparity value in each row of an image. However, the V-disparity-based method can falsely detect a non-road area as a road when the disparity value is not accurate or the disparity value of the object is equal to the disparity value of the road. In a road environment including vegetation, such as a highway and a country road, the vegetation area may be falsely detected as the drivable area because the disparity characteristics of the vegetation are similar to those of the road. Therefore, this paper proposes a drivable area detection method and hardware architecture with a high accuracy in road environments including vegetation areas by reducing the number of false detections caused by V-disparity characteristic. When 289 images provided by KITTI road dataset are used to evaluate the road detection performance of the proposed method, it shows an accuracy of 90.12% and a recall of 97.96%. In addition, when the proposed hardware architecture is implemented on the FPGA platform, it uses 8925 slice registers and 7066 slice LUTs.

Overexpression of cholinergic receptor nicotinic gamma subunit inhibits proliferation and differentiation of bovine preadipocytes

  • Jiawei, Du;Hui, Zhao;Guibing, Song;Yuan, Pang;Lei, Jiang;Linsen, Zan;Hongbao, Wang
    • Animal Bioscience
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    • v.36 no.2
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    • pp.200-208
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    • 2023
  • Objective: Muscle acetylcholine receptors have five alpha subunits (α, β, δ, ε, or γ), and cholinergic receptor nicotinic gamma subunit (CHRNG) is the γ subunit. It may also play an essential role in biological processes, including cell differentiation, growth, and survival, while the role of CHRNG has not been studied in the literature. Therefore, the purpose of this study is to clarify the effect of CHRNG on the proliferation and differentiation of bovine preadipocytes. Methods: We constructed a CHRNG overexpression adenovirus vector and successfully overexpressed it on bovine preadipocytes. The effects of CHRNG on bovine preadipocyte proliferation were detected by Edu assay, cell counting Kit-8 (CCK-8), real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), Western blot and other techniques. We also performed oil red O, RT-qPCR, Western blot to explore its effect on the differentiation of preadipocytes. Results: The results of Edu proliferation experiments showed that the number of EDU-positive cells in the overexpression group was significantly less. CCK-8 experiments found that the optical density values of the cells in the overexpression group were lower than those of the control group, the mRNA levels of proliferating cell nuclear antigen (PCNA), cyclin A2 (CCNA2), cyclin B1 (CCNB1), cyclin D2 (CCND2) decreased significantly after CHRNG gene overexpression, the mRNA levels of cyclin dependent kinase inhibitor 1A (CDKN1A) increased significantly, and the protein levels of PCNA, CCNB1, CCND2 decreased significantly. Overexpression of CHRNG inhibited the differentiation of bovine preadipocytes. The results of oil red O and triglyceride determination showed that the size and speed of lipid droplets accumulation in the overexpression group were significantly lower. The mRNA and protein levels of peroxisome proliferator activated receptor gamma (PPAR class="checkNonKBPoint">γ), CCAAT enhancer binding protein alpha (CEBPα), fatty acid binding protein 4 (FABP4), fatty acid synthase (FASN) decreased significantly. Conclusion: Overexpression of CHRNG in bovine preadipocytes inhibits the proliferation and differentiation of bovine preadipocytes.

Growth performance, carcass traits, muscle fiber characteristics and skeletal muscle mRNA abundance in hair lambs supplemented with ferulic acid

  • Pena-Torres, Edgar Fernando;Castillo-Salas, Candelario;Jimenez-Estrada, Ismael;Muhlia-Almazan, Adriana;Pena-Ramos, Etna Aida;Pinelli-Saavedra, Araceli;Avendano-Reyes, Leonel;Hinojosa-Rodriguez, Cindy;Valenzuela-Melendres, Martin;Macias-Cruz, Ulises;Gonzalez-Rios, Humberto
    • Journal of Animal Science and Technology
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    • v.64 no.1
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    • pp.52-69
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    • 2022
  • Ferulic acid (FA) is a phytochemical with various bioactive properties. It has recently been proposed that due to its phytogenic action it can be used as an alternative growth promoter additive to synthetic compounds. The objective of the present study was to evaluate the growth performance, carcass traits, fiber characterization and skeletal muscle gene expression on hair-lambs supplemented with two doses of FA. Thirty-two male lambs (n = 8 per treatment) were individually housed during a 32 d feeding trial to evaluate the effect of FA (300 and 600 mg d-1) or zilpaterol hydrochloride (ZH; 6 mg d-1) on growth performance, and then slaughtered to evaluate the effects on carcass traits, and muscle fibers morphometry from Longissimus thoracis (LT) and mRNA abundance of β2-adrenergic receptor (β2-AR), MHC-I, MHC-IIX and IGF-I genes. FA increased final weight and average daily gain with respect to non-supplemented animals (p < 0.05). The ZH supplementation increased LT muscle area, with respect to FA doses and control (p < 0.05). Cross-sectional area (CSA) of oxidative fibers was larger with FA doses and ZH (p < 0.05). Feeding ZH increased mRNA abundance for β2-AR compared to FA and control (p < 0.05), and expression of MHC-I was affected by FA doses and ZH (p < 0.05). Overall, FA supplementation of male hair lambs enhanced productive variables due to skeletal muscle hypertrophy caused by MHC-I up-regulation. Results suggest that FA has the potential like a growth promoter in lambs.

Quantitative Evaluation of Leak Index from Electrical Resistivity and Induced Polarization Surveys in Embankment Dams (전기비저항 및 유도분극 탐사에 의한 저수지 누수지수 산출)

  • Cho, In Ky;Kim, Yeon Jung;Song, Sung Ho
    • Geophysics and Geophysical Exploration
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    • v.25 no.3
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    • pp.120-128
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    • 2022
  • There are 17,000 reservoir dams in Korea, of which more than 85% were built over 50 years ago. Old embankment dams are weakened by internal erosion and suffusion phenomena due to preferential leakage paths and this ongoing weakening can cause their failure. Therefore, early warning associated with leakage in an embankment dam is crucial to prevent its failure. An electrical resistivity survey is a non-destructive, real-time and in-situ technique for detecting the development of leakage zones and general conditions of embankment dams. Because of its advantages, the electrical resistivity survey is widely used for reservoir safety inspections. However, the electrical resistivity survey is still not officially included in the precise safety inspection of reservoir dams because it cannot present a quantitative index of dam safety. In this study, we propose a method for calculating the leak index according to the water content evaluated from the electrical resistivity survey and/or induced polarization survey. Particularly, by proposing a quantitative leak index calculation method from monitoring surveys and independent surveys, we provide a theoretical basis for including electrical resistivity and induced polarization surveys as components of the precise safety inspection of reservoirs dams.

A Study on Implementing BSC in the Public Enterprises : The Case of Korea Southern Power (공기업의 BSC 구축에 관한 연구: 한국남부발전(주) 사례를 중심으로)

  • Suh, Woo-Jong;Park, Jin-Bae;Hong, Jin-Won
    • Journal of Korea Society of Industrial Information Systems
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    • v.14 no.4
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    • pp.163-182
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    • 2009
  • The BSC(Balanced Scorecard), a strategic performance evaluation system, has drawn attention as an innovative tool for improving an organization's performance. Recently, the Korean government has recognized the advantages of the BSC and encouraged public enterprises to implement the BSC. However, it has been pointed out that many public enterprises have faced difficulties in constructing and operating the BSC due to lack of clear understanding, a complex environment of performance evaluation, and inherent features of organizational culture. Therefore, this study analyzed a project case of a public enterprise, Korea Southern Power (KSP), which has ever been assessed as an excellent organization in implementing BSC and managing performance. This paper provides procedures, activities, resources (manpower and time), and decision-making issues and criteria required for implementing BSC, along with real project outcomes of the company. Such project details are expected to be used as helpful guidelines for public or non-profit organizations's BSC implementation. Furthermore, the KSP's efforts to cope with its problems and implications derived from the efforts are also expected to help other organizations construct and operate the BSC effectively.

Identification of Plant Response to the Human Behavior of Crushing Plants

  • Kim, Kwang Jin;Kim, Hyeon Ju;Son, Deokjoo;Jeong, Na Ra;Yun, Hyung Gewon;Han, Seung Won;You, Soojin;Kim, Chan-joong;Lee, Seon Hwa
    • Journal of People, Plants, and Environment
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    • v.22 no.6
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    • pp.593-600
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    • 2019
  • We identified how plants affected by the human behavior of crushing plants respond and what kind of plants responded sensitively. We investigated Lactuca sativa "Gaesebadak", Syneilesis palmata and Peucedanum japonicum as plants that humans use for edible purposes, and Achyranthes japonica and Bidens bipinnata as wild plants that stick to people's clothes and disperse seed. Plants exposed to human breathing air were compared with those exposed to human breathing air after being crushed. Methyl jasmonate (MeJA), a chemical word, was measured using Syft/MS, which detects real-time VOC, and related genes were analyzed. The amount of MeJA of Syneilesis palmata and Peucedanum japonicum as edible plants was greater than that of non-edible plants that disperse seeds using humans. The amount of MeJA ranged from 0.20 ppb to 0.35 ppb when the control group were not exposed to human breathing air. On the other hand, MeJA decreased after increasing for the first hour in human breathing air. Also, MeJA affected by human breathing after crushing plants was higher than that affected by just human breathing air. Peucedanum japonicum showed the most distinctive difference between the treatment with human breathing after crushing plants and the treatment with just human breathing. In addition, the gene activity of JAR1 and JMT increased 3 hours after the treatment with human breathing after crushing plants. Therefore, in the treatment with human breathing after crushing plants, the concentration of MeJA and the activity of related genes showed the same tendency to increase. As a result, the plant that responded sensitively to human behavior was Peucedanum japonicum. Plants released MeJA as a chemical word in the treatment with human breathing air after crushing plants.

A Parametric Study for Jointed Rock Slope Using FEM (절리 암반사면에서의 인자효과에 의한 유한요소 해석의 타당성 검토)

  • Lee, Jin-A;Chung, Chang-Hee;Chun, Byung-Sik
    • Journal of the Korean Geotechnical Society
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    • v.23 no.6
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    • pp.97-102
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    • 2007
  • Though the stability analysis of soil slopes widely employs the limit equilibrium method, the study on the jointed rock slopes must consider the direction of joint and the characteristics of Joint at the same time. This study analyzes the result of the change in the factors which show the characteristics of discontinuity and the shape factor of rock slopes, and so on, in an attempt to validate the propriety as to the interpretation of jointed rock slope stability which uses the general finite element program. First, the difference depending on the flow rules was compared, and the factor effect study was conducted. The selected independent variables included the direction of joint which displays the mechanical characteristics of discontinuity, adhesive cohesion, friction angle, the inclination and height of rock slope which reveal the shape of slope and surcharge load. And the horizontal displacement was numerically interpreted at the 1/3 point below the slope, a dependent variable, to compare the relative degree of factor effects. The findings of study on factor effects led to the validation that the result of horizontal displacement for each factor satisfied various engineering characteristics, making it possible to be applied to stability interpretation of jointed rock slope. A modelling is possible, which considers the application of the result of real geotechnical surveys & laboratory studies and the non-linear characteristics when designing the rock slope. In addition, the stress change which may result from the natural disaster, such as precipitation, and the construction, can be expressed. Furthermore, as the complicated rock condition and the ground supporting effect can be considered through FEM, it is considered to be very useful in making an engineering decision on the cut-slope, reinforcement and so on.

miR-380-3p promotes β-casein expression by targeting αS1-casein in goat mammary epithelial cells

  • Ning Song;Jun Luo;Lian Huang;Xiaoying Chen;Huimin Niu;Lu Zhu
    • Animal Bioscience
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    • v.36 no.10
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    • pp.1488-1498
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    • 2023
  • Objective: αS1-Casein is more closely associated with milk allergic reaction than other milk protein components. microRNA (miRNA) is a class of small non-coding RNAs that modulate multiple biological progresses by the target gene. However, the post-transcriptional regulation of αS1-casein expression by miRNA in ruminants remains unclear. This study aims to explore the regulatory roles of miR-380-3p on αS1-casein synthesis in goat mammary epithelial cells (GMEC). Methods: αS1-Casein gene and miR-380-3p expression was measured in dairy goat mammary gland by quantitative real-time polymerase chain reaction (qRT-PCR). miR-380-3p overexpression and knockdown were performed by miR-380-3p mimic or inhibitor in GMEC. The effect of miR-380-3p on αS1-casein synthesis was detected by qRT-PCR, western blot, luciferase and chromatin immunoprecipitation assays in GMEC. Results: Compared with middle-lactation period, αS1-casein gene expression is increased, while miR-380-3p expression is decreased during peak-lactation of dairy goats. miR-380-3p reduces αS1-casein abundance by targeting the 3'-untranslated region (3'UTR) of αS1-casein mRNA in GMEC. miR-380-3p enhances β-casein expression and signal transducer and activator of transcription 5a (STAT5a) activity. Moreover, miR-380-3p promotes β-casein abundance through target gene αS1-casein, and activates β-casein transcription by enhancing the binding of STAT5 to β-casein gene promoter region. Conclusion: miR-380-3p decreases αS1-casein expression and increases β-casein expression by targeting αS1-casein in GMEC, which supplies a novel strategy for reducing milk allergic potential and building up milk quality in ruminants.

A Study on the Development of Traffic Accident Information System Based on WebGIS (WebGIS 기반 교통사고정보관리 시스템 개발에 관한 연구)

  • Jeong, Su-Jin;Lim, Seung-Hyeon;Cho, Gi-Sung
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.6D
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    • pp.1003-1010
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    • 2006
  • This study developed a traffic accident information management system based on WebGIS that can process a lot of data for giving effectively diagnosis of traffic accidents in serious damage circumstances by traffic accident. Also, this study presents a way to compose and to convey traffic accident information. In addition, non-spatial attributes as well as spatial attributes about traffic accidents information be integrated and managed by the system. To provide Web service, we developed modules that can supply visually spatial information and traffic accidents data through ASP, Javascript, ArcIMS based on Web and constructed a server. And constructed system include a function that offer the now situation of traffic accident in real time, which supply the statistical data of traffic accident through Web as soon as user entry data in comparison with previous way that preparatory period until traffic accidents data is supplied to peoples had been long. Traffic accidents are analyzed with only nonspatial attribute by simply collecting in the past. However, system constructed by this study offer new function that can grasp visually accident spot circumstance and use detailed content and accurate location data as well as statistical data of traffic accidents. Also, it offer interface that can connect directly with accident charge policeman.

Comparison of miR-106b, miR-191, and miR-30d expression dynamics in milk with regard to its composition in Holstein and Ayrshire cows

  • Marina V. Pozovnikova;Viktoria B. Leibova;Olga V. Tulinova;Elena A. Romanova;Artem P. Dysin;Natalia V. Dementieva;Anastasiia I. Azovtseva;Sergey E. Sedykh
    • Animal Bioscience
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    • v.37 no.6
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    • pp.965-981
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    • 2024
  • Objective: Milk composition varies considerably and depends on paratypical, genetic, and epigenetic factors. MiRNAs belong to the class of small non-coding RNAs; they are one of the key tools of epigenetic control because of their ability to regulate gene expression at the post-transcriptional level. We compared the relative expression levels of miR-106b, miR-191, and miR-30d in milk to demonstrate the relationship between the content of these miRNAs with protein and fat components of milk in Holstein and Ayrshire cattle. Methods: Milk fat, protein, and casein contents were determined in the obtained samples, as well as the content of the main fatty acids (g/100 g milk), including: saturated acids, such as myristic (C14:0), palmitic (C16:0), and stearic (C18:0) acids; monounsaturated acids, including oleic (C18:1) acid; as well as long-, medium- and short-chain, polyunsaturated, and trans fatty acids. Real-time stem-loop one-tube reverse transcription polymerase chain reaction with TaqMan probes was used to measure the miRNA expression levels. Results: The miRNA expression levels in milk samples were found to be decreased in the first two months in Holstein breed, and in the first four months in Ayrshire breed. Correlation analysis did not reveal any dependence between changes in the expression level of miRNA and milk fat content, but showed a multidirectional relationship with individual milk fatty acids. Positive associations between the expression levels of miR-106b and miR-30d and protein and casein content were found in the Ayrshire breed. Receiver operating characteristic curve analysis showed that miR-106b and miR-30d expression levels can cause changes in fatty acid and protein composition of milk in Ayrshire cows, whereas miR-106b expression level determines the fatty acid composition in Holsteins. Conclusion: The data obtained in this study showed that miR-106b, miR-191, and miR-30d expression levels in milk samples have peculiarities associated with breed affiliation and the lactation period.