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Distinctive response of maize (Zea mays L.) genotypes in vitro with the acceleration of phytohormones

  • Muppala, Sridevi;Gudlavalleti, Pavan Kumar;Pagidoju, Sreenu;Malireddy, Kodandarami Reddy;Puligandla, Sateesh Kumar;Dasari, Premalatha
    • Journal of Plant Biotechnology
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    • v.47 no.1
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    • pp.26-39
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    • 2020
  • In maize, immature embryos (IEs) are highly regenerative explants most suitable for producing high frequencies of plantlet regeneration in vitro. Apart from media, explants, and hormones, genotypic variation also influences in vitro characters to a great extent. In the present study, IEs were used to study the distinctive effect of variation of size/stage and hormones in different genotypes on five in vitro characters viz., frequency of callus induction, growth rate of total callus, frequency of E. callus induction, and volume and number of regenerated plantlets. LS medium with different concentrations of 2,4-D (0.5, 1.5, 2.5, 4.0 and 5.0 mg/L) were used to study the former four in vitro characters, and medium with 6-benzylaminopurine and kinetin (0.5 mg/L, each) was used for plantlet regeneration. IEs of 1.0, 1.5, 2.0, 2.5 and 3.0 mm in size were isolated from four inbred lines viz., NM74C, NM81A, NM5883 and NM5884. Two-way ANOVA revealed that explant size and genotypes, as well as hormonal concentrations showed significant effects on in vitro characters. Two millimeter IEs were found to be suitable for in vitro cultures. LS medium with 1.5 mg/L 2,4-D and LS with BAP and Kn (0.5 mg/L, each) were found to be the best hormonal concentrations for callus induction, maintenance, and regeneration, respectively. Among the four genotypes, NM81A and NM5883 yielded more non-embryogenic and Type I E. calli. In contrast, NM74C and NM5884 yielded more highly regenerative Type II calli. Inbred line NM5884 was found to be the best among these four genotypes.

Diverse patterns of bone regeneration in rabbit calvarial defects depending on the type of collagen membrane

  • Hong, Inpyo;Khalid, Alharthi Waleed;Pae, Hyung-Chul;Song, Young Woo;Cha, Jae-Kook;Lee, Jung-Seok;Paik, Jeong-Won;Choi, Seong-Ho
    • Journal of Periodontal and Implant Science
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    • v.51 no.1
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    • pp.40-52
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    • 2021
  • Purpose: Various crosslinking methods have been introduced to increase the longevity of collagen membranes. The aim of this study was to compare and evaluate the degradation and bone regeneration patterns of 3 collagen membranes. Methods: Four 8-mm-diameter circular bone defects were created in the calvaria of 10 rabbits. In each rabbit, each defect was randomly allocated to 1) the sham control group, 2) the non-crosslinked collagen sponge (NS) group, 3) the chemically crosslinked collagen membrane (CCM) group, or 4) the biphasic calcium phosphate (BCP)-supplemented ultraviolet (UV)-crosslinked collagen membrane (UVM) group. Each defect was covered with the allocated membrane without any graft material. Rabbits were sacrificed at either 2 or 8 weeks post-surgery, and radiographic and histologic analyses were done. Results: New bone formed underneath the membrane in defects in the CCM and UVM groups, with a distinctive new bone formation pattern, while new bone formed from the base of the defect in the NS and control groups. The CCM maintained its shape until 8 weeks, while the UVM and NS were fully degraded at 8 weeks; simultaneously, sustained inflammatory infiltration was found in the margin of the CCM, while it was absent in the UVM. In conclusion, the CCM showed longer longevity than the UVM, but was accompanied by higher levels of inflammation. Conclusions: Both the CCM and UVM showed distinctive patterns of enhancement in new bone formation in the early phase. UV crosslinking can be a biocompatible alternative to chemical crosslinking.

The Differences in Smartphone Addiction Symptom between Highly Addicted and Non-addicted College Students (스마트폰 고중독 대학생과 비중독 대학생의 중독증후군 차이)

  • Im, Kyungja;Suh, Namrye;Byun, Juna
    • Journal of the Korean Society of School Health
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    • v.27 no.3
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    • pp.189-195
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    • 2014
  • Purpose: This survey was to investigate the differences in smartphone addiction symptoms by the level of addiction among college students in S. Korea. Methods: Two hundred and thirteen university students survey data was collected from December 5th to 9th of 2011 using Smartphone Addiction Scale. 25.3% (n=54) of total respondents were categorized as highly addicted and 28.17% (n=60) were as non-addicted group. Results: There were significant differences in total addiction symptom scores, daily life disorders, loss of self control, pathological concentration, and compulsive symptoms between two groups. There were significantly differences in total addiction symptoms, daily life disorders, loss of self control, pathological concentration, and compulsive symptoms between two groups. Highly addicted group were significantly higher in total addiction symptom scores (3.26 times), daily life disorders (4.96 times), loss of self control (3.07 times), pathological concentration (3.07 times), and compulsive symptoms (2.86 times) compared to non-addicted one. Highly addicted group had significantly higher daily usages of smartphone and were more satisfied with smartphone than non-addicted group. The purpose to use was related to academic works rather than games or entertainment. However satisfaction on academic achievement was very low. Conclusion: The smartphone addicted symptoms related to academic works were significantly high in highly addicted college students so that it disturbed their daily life and psycho-social health in general. It is clear that the highly addicted college students need urgent distinctive interventions such as creative academic guides.

A Subjectivity Study on Nonverbal Communication Cues in Facebook (온라인에서의 비언어적 요소에 대한 주관성 연구: 페이스북을 중심으로)

  • Kim, Suhyeong;Lee, Jungwoo
    • Journal of Digital Convergence
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    • v.18 no.2
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    • pp.183-195
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    • 2020
  • Non-verbal communication cues seem to be increasingly used in social media, such as emoticon, like, follow, etc. A Q methodology study is conducted to explore this phenomenon. Via a series of focus group activities with Facebook heavy users, 34 Q statements concerning online non-verbal communication cues are derived. Different Facebook users conduct Q sorts of these statements based on frequent use basis. Analysis of Q sort result reveals three distinctive usage patterns of non-verbal communication cues in Facebook: 'safe distance keeping' type, 'observing hermit' type and 'fully dedicated aficionado' type. Discussions are made concerning different components of non-verbal communication cues utilized in these groups, respectively, which can provide a theoretical basis for social media design.

An Investigation on Non-Relevance Criteria for Image in Failed Image Search (이미지 검색 실패에 나타난 비적합성 평가요소 규명에 관한 연구)

  • Chung, EunKyung
    • Journal of the Korean Society for Library and Information Science
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    • v.50 no.1
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    • pp.417-435
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    • 2016
  • Relevance judgment is important in terms of improving the effectiveness of information retrieval systems, and it has been dominant for users to search and use images utilizing internet and digital technologies. However, in the field of image retrieval, there have been only a few studies in terms of identifying relevance criteria. The purpose of this study aims to identify and characterize the non-relevance criteria from the failed image searches. In order to achieve the purpose of this study, a total of 135 participants were recruited and a total of 1,452 criteria items were collected for this study. Analyses and identification on the data set found thirteen criteria such as 'topicality', 'visual content', 'accuracy', 'visual feature', 'completeness', 'appeal to user', 'focal point', 'bibliographic information', 'impression', 'posture', 'face feature', 'novelty', and 'time frame'. Among these criteria, 'visual content' and 'focal point' were introduced in this current study, while 'action' criterion identified in previous studies was not shown in this current study. When image needs and image uses are analyzed with these criteria, there are distinctive differences depending on different image needs and uses.

Intra-and Inter-frame Features for Automatic Speech Recognition

  • Lee, Sung Joo;Kang, Byung Ok;Chung, Hoon;Lee, Yunkeun
    • ETRI Journal
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    • v.36 no.3
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    • pp.514-517
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    • 2014
  • In this paper, alternative dynamic features for speech recognition are proposed. The goal of this work is to improve speech recognition accuracy by deriving the representation of distinctive dynamic characteristics from a speech spectrum. This work was inspired by two temporal dynamics of a speech signal. One is the highly non-stationary nature of speech, and the other is the inter-frame change of a speech spectrum. We adopt the use of a sub-frame spectrum analyzer to capture very rapid spectral changes within a speech analysis frame. In addition, we attempt to measure spectral fluctuations of a more complex manner as opposed to traditional dynamic features such as delta or double-delta. To evaluate the proposed features, speech recognition tests over smartphone environments were conducted. The experimental results show that the feature streams simply combined with the proposed features are effective for an improvement in the recognition accuracy of a hidden Markov model-based speech recognizer.

Fine Needle Aspiration Cytology of Papillary Renal Cell Carcinoma - A Case Report - (유두형 신세포암종의 세침흡인 세포학적 소견 - 1예 보고 -)

  • Woo, Yeong-Ju;Kim, Sung-Sook;Lee, Jong-Wha
    • The Korean Journal of Cytopathology
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    • v.9 no.1
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    • pp.95-98
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    • 1998
  • Papillary renal cell carcinoma (RCC) is an uncommon subtype of RCC that has distinctive gross, histologic, and cytogenetic features. The cytologic features of FNA are abundant papillary clusters and relatively few single cells. The cells are usually small and contain uniform nuclei; numerous macrophages with foamy cytoplasm are often found in the background. We describe a case of papillary renal cell carcinoma evaluated by fine needle aspiration cytology(FNAC) in a 42 year-old man. The smear showed a few papillary clusters and numerous macrophages with foamy cytoplasm in the background. With adequate cellularity, papillary RCC can be distinguished reliably from non-papillary RCC by FNAC.

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Molecular Biological Characteristics of Ustilago maydis Virus Isolated in Korea

  • Won, Yie-Se;Choi, Hyoung-Tae
    • Korean Journal of Microbiology
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    • v.30 no.3
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    • pp.177-180
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    • 1992
  • Among 120 U. maydis strains isolated in Korea 14 different strains containing specific viral dsRNA segments were analyzed for the distribution of dsRNA and the production of toxin protein. Several distinctive dsRNA patterns were identified, 9 cases of P type with typical H, M and L ds RNA and one case of non-P-type, the frequency of a specific isolate was decreased with increasing number of dsRNA segments. The presence of dsRNA had no effect on the cultural or morphological phenotype of the host. Two isolates containing P type dsRNA segments appeared to produce toxin protein (killer strains) which inhibited the growth of 4 isolates (sensitive strain) with different susceptibility. Two killer strains contain unique M dsRNA segment which may code for toxin protein. However, the presence of toxin-sensitive strains among dsRNA-free isolates was similar to that of ds RNA containing strains.

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The Occurrance of Velogenic Viscerotropic Newcastle Disease Virus in an Adult Peacock (성숙 공작(Pavo cristatus)에서 발생한 내장 친화형 뉴캣슬병 바이러스 강독주)

  • 조경오;박남용;강문일;고홍범;이근우
    • Journal of Veterinary Clinics
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    • v.18 no.2
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    • pp.152-155
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    • 2001
  • A two-year-old male peacock (Pavo cristatus) showed acute watery green diarrhea, followed by neurological signs including torticollis and muscular tremor. By the hemagglutination inhibition test for detecting the antibody against the Newcastle disease virus (NDV), the peacock serum inhibited the agglutination of chicken red blood cells. Grossly distinctive hemorrhagic lesions were found in the mucosa of proventiculus and intestine and lung. The spleen revealed multiple variable sized necrotic foci. Histologically, the mucosa of gastrointestinal track had hemorrhagic lesions and some of them underwent ulceration. The spleen exhibited multiple variable sized necrotic foci in which fibrin exudation was marked. Central nervous system had mild non-suppulative menin-goencephalitis consisting of vasculitis, perivascular hemorrhage, gliosis and meningitis. The cells particularly in the cerebellum were degenerative to necrotic. Some of these nerve cells revealed characteristic peripheral chromatolysis. From the present serological and pathological findings, it is suggested that NDV causing death of peacock was velogenic viscerotropic strain. This is the first report of the occurrence of velogenic viscerotropic NDV in an adult peacock in Korea.

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Urease를 이용한 위점막 시료에서 Helicobacter pylori의 신속한 진단법

  • Lee, Hak-Seong;No, Im-Hwan;Choe, Tae-Bu;Lee, Jong-Hwa
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.264-265
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    • 2000
  • Helicobacter pylori(H. pylori) is the causative agent of chronic gastritis and the single most important factor in peptic ulcer disease, however, the pathogenetic mechanisms underlying H, pylori infection are not well understood. Futhermore, there is a strong association between H. pylori infection and gastric cancer. Various diagnostic methods for detecting H. pylori infection are available. These can be divided into invasive methods, requiring endoscopy, and non-invasive tests, mainly 13C-urea breath tests and serologic detection of antibodies. Rapid urease test is the most recommendable endoscopic test for the diagnosis of H. pylori infection, presently. CLO test kit is the represent of rapid urease test kits. The principles of CLO test kit is that hydrolysis of urea by urease Is detected by a dye indicators showing a color change. Our device is used same principle but we improved the reaction time is more faster and positive color change is more distinctive from the color of the negative specimen. So, this kit is more reliable because it response faster and accuracy.

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