• Title/Summary/Keyword: Node culture

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Design and Implementation of local advertising application(App) through SNS service analysis (SNS(Facebook) 서비스 분석을 통한 지역광고 어플(App) 설계 및 구현)

  • Cho, Young-Sik
    • Journal of Digital Contents Society
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    • v.16 no.2
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    • pp.325-334
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    • 2015
  • In this paper, we had tried to study advertising method of local industry in cooperation with SNS (Social Network Service). In particular, This paper analyzes the Facebook Page trying to find a way to take advantage of the local advertising. To analyse the Facebook page, I had created three Facebook pages such as at Hongik UNIV., Gangwon National UNIV., and specialities in Cuncheon cities. While I was managing the three pages, I had analyzed the results of quantities of activities, and in addition, had used NodeXL for the analysis of the network for the each page. After the analysis of the data of each page, I had found the possibility of the promotion for the successful the local advertizement associated with SNS. To verify and develop the results of my study, I had designed the " real time local advertising APP which is connected with SNS like Facebook, had created server program based on PHP, Android APP. In the future, I have found that there must be a lot of possibilities for the successful and various the local advertising methodologies by using SNS like Facebook, the operational data of the App.

DL-LEACH: Hierarchical Dual-Hop Routing Protocol for Wireless Sensor Network (DL-LEACH : 무선 센서 네트워크를 위한 계층형 멀티 홉 라우팅 프로토콜)

  • Lee, Chang-Hee;Lee, Jong-Yong
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.15 no.5
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    • pp.139-145
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    • 2015
  • This paper proposes to increase the node energy effienciecy, which rapidly drops during the transmission of LEACH (Low Energy Adaptive Clustering Hierachy), using the method of DL-LEACH (Dual-hop Layered LEACH). By introducing dual-hop method in the data transmission, the proposed single-hop method for short-range transmission and multi-hop transmission method between the cluster heads for remote transmission was introduce. By introducing a partial multi-hop method in the data transmission, a single-hop method for short range transmission method between the cluster heads for remote transmission was introduces. In the proposed DL-LEACH, the energy consumption of cluster head for remote transmission reduces and increases the energy efficiency of sensor node by reducing the transmission distance and simplifying the transmission routine for short-range transmission. As compared the general LEACH, it was adapted to a wider sensor field.

Correlation between in vitro Flowering Frequency and the Structure of Cytokinins in Ginseng (Panax ginseng C.A. Meyer) (인삼 (Panax ginseng C.A. Meyer)의 기내 화아형성 빈도와 cytokinin 구조와의 관계)

  • 이행순;김윤성;권석윤;곽상수;유장렬
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.2
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    • pp.109-113
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    • 1999
  • To elucidate the relationship between in vitro flowering and the structure of cytokinins in ginseng (Panax ginseng C.A. Meyer), zygotic embryos, seedlings, and cotyledonary nodes were cultured on MS medium supplemented with 5 $\mu$M of various cytokinins (BA, kinetin, 2-iP, and zeatin) with or without GA$_3$ (5 $\mu$M). The frequency of in vitro flowering was the highest when explants were cultured on the medium containing BA regardless of the kinds of explants, followed by kinetin, 2-iP, and zeatin. Flowering frequency of cotyledonary node explants was significantly increased by the combined treatment of cytokinin and GA$_3$. Flowering frequency was highly correlated with the logP of cytokinins, indicating that the lipophilicity of each cytokinin may involved in the in vitro flowering of ginseng.

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Effects of Light-emitting Diodes on In Vitro Growth of Virus-free Sweet Potato Plantlets (LED가 고구마 바이러스 무병묘의 기내 생장에 미치는 영향)

  • Yoo, Kyoung-Ran;Lee, Seung-Yeob
    • Horticultural Science & Technology
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    • v.35 no.4
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    • pp.490-498
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    • 2017
  • The in vitro growth of virus-free sweet potato [Ipomoea batatas (L.) Lam.] plantlets was investigated under different light sources: fluorescent lamp (control); red (660 nm), blue (460 nm), white light-emitting diodes (LED), and two mixtures of blue and red LED (R:B = 8:2, and 7:3). Single node explants (10 mm) of three cultivars ('Matnami', 'Shincheonmi', and 'Yeonhwangmi') were cultured on Murashige and Skoog medium supplemented with $0.2mg{\cdot}L^{-1}$ 6-benzyladenine for 4 weeks. Explants were exposed to $150{\pm}5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux at a distance of 20 cm, constant temperature of $25^{\circ}C$, and under 16/8-h (day/night) photoperiod. Using the same method, the in vitro growth of 10 cultivars under red LED was also compared. After 3 weeks, vine length was highest in plantlets cultured under red LED, and lowest in plantlets cultured under blue LED. Fresh and dry weights were also greatest in plantlets cultured under red LED. Compared to the control, vine thickness was significantly higher in plantlets grown under white LED and the 7:3 R:B LED mixture. Significant differences were observed among the 10 cultivars grown under red LED. 'Matnami', 'Shincheonmi', and 'Shinhwangmi' all had excellent vine lengths, and fresh and dry weights. Compared to the control, vine elongation of sweet potato plantlets was most effective under red LED, and culture duration was about 1 week shorter.

Fibroblastic Reticular Cell Derived from Lymph Node Is Involved in the Assistance of Antigen Process (림프절 유래 fibroblastic reticular cell의 효율적 항원처리 관련성에 대한 연구)

  • Kim, Min Hwan;Lee, Jong-Hwan
    • Journal of Life Science
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    • v.26 no.9
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    • pp.1027-1032
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    • 2016
  • Antigen is substance causing disease derived from pathogen. Living organism has the immune system in terms of defense mechanism against antigen. Antigen is processed through several pathways such as phagocytosis, antibody action, complement activation, and cytotoxins by NK or cytotoxic T lymphocyte via MHC molecule. Lymph node (LN) is comprised of the complicated 3 dimensional network and several stromal cells. Fibroblastic reticular cells (FRC) are distributed in T zone for interaction with T cells. FRC produces the extra cellular matrix (ECM) into LN for ECM reorganization against pathogen infections and secretes homing chemokines. However, it has not so much been known about the involvement of the antigen process of FRC. The present report is for the function of FRC on antigen process. For this, FRC was positioned with several infected situations such as co-culture with macrophage, T cell, lipopolysaccharide (LPS) and TNFα stimulation. When co-culture between FRC with macrophage and T cells was performed, morphological change of FRC was observed and empty space between FRCs was made by morphological change. The matrix metallo-proteinase (MMP) activity was up-regulated by Y27632 and T cells onto FRC. Furthermore, inflammatory cytokine, TNFα regulated the expression of adhesion molecules and MHC I antigen transporter in FRC by gene chip assay. NO production was elevated by FRC monolayer co-cultured with macrophage stimulated by LPS. GFP antigen was up-taken by macrophage co-cultured with FRC. Collectively, it suggests that FRC assists of the facilitation of antigen process and LN stroma is implicated into antigen process pathway.

Yield and Size of Enlarged Rhizome among Different Kinds of White Lotus (백련 종류에 따른 연근 크기와 수확량)

  • Im, Myung-Hee;Park, Yong-Seo;Cho, Ja-Yong;Park, Sam-Gyun;Yun, Jae-Gill;Jang, Hong-Gi;Heo, Buk-Gu
    • Korean Journal of Plant Resources
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    • v.22 no.1
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    • pp.42-48
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    • 2009
  • This study was conducted to examine the difference in yield and size of enlarged rhizome according to the kinds of native white lotuses. Four white lotuses (Nelumbo nucifera) were collected from 4 different sites, Jeonbuk Jeonju ('Garam'), Jeonnam Muan ('Hoesan'), Chungnam Buyeo ('Ihnchuisa'), and Chungnam Cheongyang('Cheongyang'). Each enlarged rhizome was planted in an area of 3.3 $m^2$ on 10. April, 2007 and digged out on 19. February, 2008. The yield of enlarged rhizome was in the order of Hoesan (3.0 kg/$m^2$), Cheongyang (2.5 kg/$m^2$), Garam (2.0 kg/$m^2$) and Ihnchuisa (1.0 kg/$m^2$). The number of enlarged rhizomes was in the order of Ihnchuisa (4.9/$m^2$), Garam (4.3/$m^2$), Hoesan (4.2/$m^2$) and Cheongyang (4.1/$m^2$). Total number of nodes in the enlarged rhizomes were much more in the order of Hoesan (17.0/$m^2$), Ihnchuisa (16.0/$m^2$), Cheongyang (15.3/$m^2$) and Garam (14.1/$m^2$). Component ratio of enlarged rhizome length below 50cm for Ihnchuisa, Garam, Hoesan and Cheongyang were 76.3%, 53.0%, 38.2% and 40.3% respectively. Native white lotus had a longer enlarged rhizome in the first node than the other nodes, and their enlarged rhizome length and width became thin. And it became thick and short from the second node of enlarged rhizome. Accordingly the amount of enlarged rhizomes 'Hoesan' was extremely much than others.

Growth Acceleration and Acclimatization of In Vitro Plantlets derived from Apical Meristem of Sweet Potato (고구마의 경정조직 유래 기내 소식물체의 생장촉진과 순화)

  • ;;Shiro Higashi
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.2
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    • pp.115-119
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    • 1999
  • The single node cuttings of sweet potato (cv. Mokpo #29) plantlets maintained in vitro were cultured with (MF+) or without membrane filter (MF-) under photomixotrophic (PM), hetrotrophic (HT) and autotrophic (AT) conditions. Shoot length was the greatest (11.9cm) in 3$0^{\circ}C$ (HT) treatment and it was the shortest (3.4 cm) in $25^{\circ}C$ (PM) treatment. Nodal explants cultured in 3$0^{\circ}C$ treatment looked more vigorous than those of $25^{\circ}C$ in appearance, and node number was the greatest (10.5 per plantlet) among the treatments. But plantlets grew in 3$0^{\circ}C$ (HT) treatment were observed all overgrown. The size in leaf area was about 2 times greater and shoot length was about 2 times shorter in PM than in HT condition. Percent dry matter of shoots was 5.9% (HT) and 7.4% (PM) in $25^{\circ}C$ treatment and 6.1% (HT) and 7.4% (PM) in 3$0^{\circ}C$ treatment. Plantlets cultured in the MF+ treatments were less succulent than those cultured in the MF- treatment. Vitrified plantlets were examinated 14.8% (both $25^{\circ}C$ and 3$0^{\circ}C$) in PM condition and 22.2% ($25^{\circ}C$) and 31.5% (3$0^{\circ}C$) in HT condition. Sucrose was necessary for the survival of in vitro plantlets. In the sucrose-free medium, explants cultured in the MF- had turned yellow and were dead after 30 days of culture. But explants cultured in the MF+ were alive and produced plantlets with shoot and root (AT). On the other hand, the survival of explants on the MS basal medium (sucrose-free and hormone-free) depended entirely upon the MF attachment.

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Characterization of the Immune Regulation Function of Fibroblastic Reticular Cells Originating from Lymph Node Stroma (림프절 스트로마 유래 fibroblastic reticular cell의 면역조절 기능에 대한 특성 규명)

  • Lee, Jong-Hwan
    • Journal of Life Science
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    • v.26 no.7
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    • pp.789-795
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    • 2016
  • A lymph node (LN) is one of the secondary lymphoid organs. An LN consists of a complicated 3 dimensional frame structure and several stromal cells. Fibroblastic reticular cells (FRC) are distributed in the T zone for interaction with T cells. FRC secrete homing chemokines such as CCL19 and CCL21. Moreover, FRC play a pivotal role in the production of extracellular matrix (ECM) into LN for ECM reorganization against pathogen infections. However, not much is known about the involvement of the immune reaction of FRC. The present report is for the characterization of FRC on immune response. For this, FRC were positioned in several infected situations such as co-culture with macrophage, lipopolysaccharide (LPS), and TNFα stimulation. When a co-culture between FRC and macrophage was performed, a morphological change in FRC was observed, and empty space between FRCs was created by this change. The soluble ICAM-1 protein level was up-regulated by co-culturing with Raw264.7 and the treatment of the ROCK inhibitor Y27632. The activity of matrix metalloproteinase (MMP) was up-regulated by LPS onto FRC. Furthermore, the inflammatory cytokine TNFα regulated the expression of ECM in FRC by a gene chip assay. Collectively, it suggests that FRC are involved in immune reactions.

Development of SDN-based Network Platform for Mobility Support (이동성 지원을 위한 SDN 기반의 네트워크 플랫폼 개발)

  • Lee, Wan-Jik;Lee, Ho-Young;Heo, Seok-Yeol
    • The Journal of the Convergence on Culture Technology
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    • v.5 no.1
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    • pp.401-407
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    • 2019
  • SDN(Softeware Defined Networking) has emerged to address the rapidly growing demand for cloud computing and to support network virtualization services. Therefor many companies and organizations have taken SDN as a next-generation network technology. However, unlike the wired network where the SDN is originally designed, the SDN in the wireless network has a restriction that it can not provide the mobility of the node. In this paper, we extended existing openflow protocol of SDN and developed SDN-based network platform, which enables the SDN controller to manage the radio resources of its network and support the mobility of the nodes. The mobility support function of this paper has the advantage that a node in the network can move using its two or more wireless interfaces by using the radio resource management function of the SDN controller. In order to test the functions implemented in this paper, we measured parameters related to various transmission performance according to various mobile experiments, and compared parameters related to performance using one wireless interface and two interfaces. The SDN-based network platform proposed in this paper is expected to be able to monitor the resources of wireless networks and support the mobility of nodes in the SDN environment.

Analysis of the Spread of Issues Related to COVID-19 Vaccine on Twitter: Focusing on Issue Salience (코로나19 백신 관련 트위터 상의 이슈 확산 양상 분석: 이슈 현저성을 중심으로)

  • Hong, Juhyun;Lee, Mina
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.4
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    • pp.613-621
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    • 2021
  • This study conducted a network analysis to determine how COVID-19 vaccine-related issue spread on Twitter during the introduction stage of the COVID-19. Issue diffusion tendency is analyzed according to the time period: phase 1 (initiation of vaccine introduction: March 7 - April 3, 2021), phase 2 (stagnant period of vaccination: April 4 - April 22, 2021), and phase 3 (increase of vaccination: April 23 - May 5, 2021). NodeXL was used for data collection and analysis. Daily Twitter network data were collected by entering search terms highly related to the COVID-19 vaccine. This study found that side effects-related opinions were repeatedly formed throughout the analysis period. As the vaccination rate increased and death cases were reported on media, death-related issues also emerged on Twitter. On the other hand, vaccine safety did not receive much attention on Twitter. The results of this study highlight the role of social media as a channel of issue diffusion when a national disaster strikes. We emphasize the need for the government to monitor public opinions on social media and reflect them in crisis communication strategies.