Browse > Article
http://dx.doi.org/10.5352/JLS.2016.26.9.1027

Fibroblastic Reticular Cell Derived from Lymph Node Is Involved in the Assistance of Antigen Process  

Kim, Min Hwan (Department of Smart Bio-Health, Dong Eui University)
Lee, Jong-Hwan (Department of Smart Bio-Health, Dong Eui University)
Publication Information
Journal of Life Science / v.26, no.9, 2016 , pp. 1027-1032 More about this Journal
Abstract
Antigen is substance causing disease derived from pathogen. Living organism has the immune system in terms of defense mechanism against antigen. Antigen is processed through several pathways such as phagocytosis, antibody action, complement activation, and cytotoxins by NK or cytotoxic T lymphocyte via MHC molecule. Lymph node (LN) is comprised of the complicated 3 dimensional network and several stromal cells. Fibroblastic reticular cells (FRC) are distributed in T zone for interaction with T cells. FRC produces the extra cellular matrix (ECM) into LN for ECM reorganization against pathogen infections and secretes homing chemokines. However, it has not so much been known about the involvement of the antigen process of FRC. The present report is for the function of FRC on antigen process. For this, FRC was positioned with several infected situations such as co-culture with macrophage, T cell, lipopolysaccharide (LPS) and TNFα stimulation. When co-culture between FRC with macrophage and T cells was performed, morphological change of FRC was observed and empty space between FRCs was made by morphological change. The matrix metallo-proteinase (MMP) activity was up-regulated by Y27632 and T cells onto FRC. Furthermore, inflammatory cytokine, TNFα regulated the expression of adhesion molecules and MHC I antigen transporter in FRC by gene chip assay. NO production was elevated by FRC monolayer co-cultured with macrophage stimulated by LPS. GFP antigen was up-taken by macrophage co-cultured with FRC. Collectively, it suggests that FRC assists of the facilitation of antigen process and LN stroma is implicated into antigen process pathway.
Keywords
Antigen process; FRC (Fibroblastic reticular cells); LPS (lipopolysaccharide); TNFα ;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Alvarenga, H. G. and Marti, L. 2014. Multifunctional roles of reticular fibroblastic cells: more than meets the eye? J. Immunol. Res. 2014:402038. doi: 10.1155/2014/402038.   DOI
2 Abdul-Cader, M. S., Amarasinghe, A. and Abdul-Careem, M. F. 2016. Activation of toll-like receptor signaling pathways leading to nitric oxide-mediated antiviral responses. Arch. Virol. Epub ahead of print.
3 Argüello, R. J., Reverendo, M., Gatti, E. and Pierre, P. 2016. Regulation of protein synthesis and autophagy in activated dendritic cells: implications for antigen processing and presentation. Immunol. Rev. 272, 28-38.   DOI
4 Bando, J. K. and Colonna, M. 2016. Innate lymphoid cell function in the context of adaptive immunity. Nat. Immunol. 17, 783-789.
5 Cooper, E. L. 2016. Commentary: Blurring borders: Innate immunity with adaptive features. Front Microbiol. 7, 358.
6 Denton, A. E., Roberts, E. W., Linterman, M. A. and Fearon, D. T. 2014. Fibroblastic reticular cells of the lymph node are required for retention of resting but not activated CD8+T cells. Proc. Natl. Acad. Sci. USA 111, 12139-12144.   DOI
7 Dingjan, I., Verboogen, D. R., Paardekooper, L. M., Revelo, N. H., Sittig, S. P., Visser, L. J., Mollard, G. F., Henriet, S. S., Figdor, C. G., Ter Beest, M. and van den Bogaart, G. 2016. Lipid peroxidation causes endosomal antigen release for cross-presentation. Sci. Rep. 6, 22064.   DOI
8 Fu, J. and Xia, L. 2016. CLEC-2 and podoplanin, partners again. Blood 127, 1629-1630.   DOI
9 Dinter, J., Gourdain, P., Lai, N. Y., Duong, E., Bracho-Sanchez, E., Rucevic, M., Liebesny, P. H., Xu, Y., Shimada, M., Ghebremichael, M., Kavanagh, D. G. and Le Gall, S. 2014. Different antigen-processing activities in dendritic cells, macrophages, and monocytes lead to uneven production of HIV epitopes and affect CTL recognition. J. Immunol. 193, 4322-4334.   DOI
10 Erler, J. T. and Weaver, V. M. 2009. Three-dimensional context regulation of metastasis. Clin. Exp. Metastasis 26, 35-49.   DOI
11 Fletcher, A. L., Acton, S. E. and Knoblich, K. 2015. Lymph node fibroblastic reticular cells in health and disease. Nat. Rev. Immunol. 15, 350-361.   DOI
12 Hirosue, S. and Dubrot, J. 2015. Modes of antigen presentation by lymph node stromal cells and their immunological implications. Front Immunol. 6, 446.
13 Klinke, D. J. 2013. An evolutionary perspective on anti-tumor immunity. Front Oncol. 2, 202.
14 Klose, C. S. and Artis, D. 2016. Innate lymphoid cells as regulators of immunity, inflammation and tissue homeostasis. Nat. Immunol. 17, 765-774.   DOI
15 Landes, M. B., Rajaram, M. V., Nguyen, H. and Schlesinger, L. S. 2015. Role for NOD2 in Mycobacterium tuberculosis- induced iNOS expression and NO production in human macrophages. J. Leukoc. Biol. 97, 1111-1119.   DOI
16 Münz, C. 2016 Autophagy proteins in antigen processing for presentation on MHC molecules. Immunol. Rev. 272, 17-27.   DOI
17 Münz, C., Steinman, R. M. and Fujii, S. 2005. Dendritic cell maturation by innate lymphocytes: coordinated stimulation of innate and adaptive immunity. J. Exp. Med. 202, 203-207.   DOI
18 Truebestein, L., Elsner, D. J., Fuchs, E. and Leonard, T. A. 2015. A molecular ruler regulates cytoskeletal remodelling by the Rho kinases. Nat. Commun. 6, 10029.   DOI
19 Nakayama, Y., Brinkman, C. C. and Bromberg, J. S. 2015. Murine fibroblastic reticular cells from lymph node interact with CD4+ T cells through CD40-CD40L. Transplantation 99, 1561-1567.   DOI
20 Takano, M., Ohkusa, M., Otani, M., Min, K. S, Kadoyama, K., Minami, K., Sano, K. and Matsuyama, S. 2015. Lipid A-activated inducible nitric oxide synthase expression via nuclear factor-κB in mouse choroid plexus cells. Immunol. Lett. 167, 57-62.   DOI
21 Weiss, G. and Schaible, U. E. 2015. Macrophage defense mechanisms against intracellular bacteria. Immunol. Rev. 264, 182-203.   DOI
22 Wu, T., McGrath, K. C. and Death, A. K. 2005. Cardiovascular disease in diabetic nephropathy patients: cell adhesion molecules as potential markers? Vasc. Health Risk Manag. 1, 309-316.   DOI