• Tuberculosis and Respiratory Diseases
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• 제42권1호
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• pp.105-109
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• 1995

저자들은 53세 여자 환자에서 Salmonella Group B에 의한 농흉 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.

• 한국의류학회지
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• 제40권2호
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• pp.203-220
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• 2016

This research analyzed characteristic changes in trading areas and the success factors of popular fashion trading areas (Garosu-gil, Dongdaemun, and Itaewon). This research adopts a social network analysis method to semantically analyze trading areas. Articles on the three fashion trading areas were located through KrKwic software to extract keywords and calculate word frequency. Keywords with high frequency were placed through NodeXL software to identify relationships among keywords. Researchers created a network of relationships among trading areas and between past and present of trading areas to analyze and visualize. In the past (2008-2009), the trading areas of Dongdaemun, Garosu-gil and Itaewon were placed horizontally with a limited number of shared keywords between them. However, the three trading areas retain diverse rage of keywords and an organic realtionships five years later (2013-2014). Compared to the past, all three areas see the emergence of relevant fashion keywords such as 'designer', 'design', 'brand'. Additional cultural keywords such as 'culture/art/performance', 'exhibition', and 'event' have commonly appeared and imply that related industries are an important factor as well. Fashion companies that consider evaluating areas for a new store opening need to understand the trading area characteristics and select the most suitable area. In addition, it is necessary to equip the trading area with basic fashion elements as well as relavant industry when the government tries to develop fashion trading areas.

• 디지털융복합연구
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• 제14권11호
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• pp.325-331
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• 2016

휴대폰 망과 같은 이동망이나 와이파이망, 주파수공용통신(Trunked Radio System)과 같은 망은 시설기반(Infra Structure)망이어서 핸드오버같은 것은 문제가 되지 않는다. 그러나 무전기 같이 수명에서 수십 명 내외의 그룹간 이동을 하면서 서로 통신을 해야 하는 경우는 망을 구성하고 있는 노드들이 실시간으로 이동하고, 토폴로지가 늘 변하고 있으며, 특히 무선으로 통신할 경우는 전파환경이 좋지 않아서 망을 구성하기란 쉽지 않다. 특히 특수목적망은 인프라 시설이 없어서 구성원들 간에 핸드오버(handover)가 필요한 경우가 많으며, 핸드오버하는 대상노드를 결정하는 것은 반드시 필요하며 중요하다. 어느 노드가 핸드오버 역할을 할지에 따라 다양한 토폴리지의 통달거리, 수신감도, 음질 등에 영향을 주게 된다. 본 고에서는 토폴로지 경우의 수를 다양하게 분석하여 전파간섭 및 핸드오버 프로토콜에 따라 데이터 전송을 원활하게 할 모델을 제시하고, 시뮬레이션을 통해 분석한 결과를 제시한다.

• 한국동물위생학회지
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• 제37권4호
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• pp.253-261
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• 2014

Porcine circovirus disease (PCVD) is a major problem of swine industry worldwide, and diagnosis of PCV2, causal agent of PCVD, has been doing in clinical laboratories of pig disease by polymerase chain reaction (PCR) methods. But the PCR analyses have a serious problem of misdiagnosis by contamination of DNA, in particular, from carryover contamination with previously amplified DNA or extracted DNA from field samples. In this study, an uracil DNA glycosylase (UNG)-based direct PCR (udPCR) without DNA extraction process and DNA carryover contamination was developed and evaluated on PCV2 culture and field pig samples. The sensitivity of the udPCR combined with dPCR and uPCR was same or better than that of the commercial PCR (cPCR) kit (Median diagnostics, Korea) on PCV2-positive serum, lymph node and lung samples of the pigs. In addition, the udPCR method confirmed to have a preventing ability of mis-amplification by contamination of pre-amplified PCV2 DNA from previous udPCR. In clinical application, 170 pig samples (86 tissues and 84 serum) were analysed by cPCR kit and resulted in 37% (63/170) of positive reaction, while the udPCR was able to detect the PCV2 DNA in 45.3% (77/170) with higher sensitivity than cPCR. In conclusion, the udPCR developed in the study is a time, labor and cost saving method for the detection of PCV2 and providing a preventing effect for DNA carryover contamination that can occurred in PCR process. Therefore, the udPCR assay could be an useful alternative method for the diagnosis of PCV2 in the swine disease diagnostic laboratories.

• 혜화의학회지
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• 제16권2호
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• pp.251-265
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• 2007

Yanhyeoljeseuptang(YHJST) is a traditional herbal medicine used for the treatment of dermatitis. The aim of this study was to confirm whether or not YHJST has a preventive effect on development of atopic dermatitis in dinitrochlorobenzene(DNCB)-applied Nc/Nga mouse. This study was undertaken to develop a reliable mouse model demonstrating similar immunologic phenomena as human atopic dermatitis characterized with predominance of type-2 immune response. NC/Nga mouse were sensitized with $200\;{\mu\ell}$ of 1% 2,4-dinitrochlorobenzene(DNCB) (acetone : olive oil = 3 : 1 mixture) and challenged twice or three times with $150\;{\mu\ell}$ of 0.2% DNCB in a week for the following 4 weeks. YHJST was administered orally to Nc/Nga mouse for 8 weeks, which led to the remarkable suppression on the development of dermatitis, as determined by various immune factors related to pathogenesis of atopic dermatitis in splenocytes and DLN cells. In this study, YHJST selectively suppressed T ce11 (CD4+, CD3+/CD69+, CD4+/CD25+) activation, which may be essential for ratio of IL-4 versus INF-$\gamma$ produced in the splenic T cell culture supernatants was approximately 3-fold higher in the mouse treated with DNCB than their control mouse respectively. Immunologic studies showed down-regulated that the capacity of spleen T cells to produce IL-4, but IFN-$\gamma$ was up-regulated by means of oral intake of these YHJST. These results strongly suggest that YHJST is a promising candidate for treatment of human atopic dermatitis.

• 혜화의학회지
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• 제16권2호
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• pp.267-280
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• 2007

Gupoongjeseuptang(GPJST) is a traditional herbal medicine used for the treatment of dermatitis. The aim of this study was to confirm whether or not GPJST has a preventive effect on development of atopic dermatitis in dinitrochlorobenzene(DNCB)-applied Nc/Nga mouse. This study was undertaken to develop a reliable mouse model demonstrating similar immunologic phenomena as human atopic dermatitis characterized with predominance of type-2 immune response. NC/Nga mouse were sensitized with $200\;{\mu\ell}$ of 1% 2,4-dinitrochlorobenzene(DNCB) (acetone : olive oil = 3 : 1 mixture) and challenged twice or three times with $150\;{\mu\ell}$ of 0.2% DNCB in a week for the following 4 weeks. GPJST was administered orally to Nc/Nga mouse for 6 weeks, which led to the remarkable suppression on the development of dermatitis, as determined by various immune factors related to pathogenesis of atopic dermatitis in splenocytes and DLN cells. In this study, GPJST selectively suppressed T ce11 (CD4+, CD3+CD69+, CD4+CD25+) activation, which may be essential for ratio of IL-4 versus INF-$\gamma$ produced in the splenic T cell culture supernatants was approximately 3-fold higher in the mouse treated with DNCB than their control mouse respectively. Immunologic studies showed down-regulated that the capacity of spleen T cells to produce IL-4, but IFN-$\gamma$ was up-regulated by means of oral intake of these GPJST. These results strongly suggest that GPJST is a promising candidate for treatment of human atopic dermatitis.

• 한국약용작물학회지
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• 제14권1호
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• pp.31-35
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• 2006

Agrobacterium rhizogenes ATCC15834에 의해 형질전환된 지황 (Rehmannia glutinosa)의 모상근으로부터 유식물체를 재분화 시키기 위한 최적 조건을 탐색하였다. 모상근 절편으로부터 유식물체의 재분화는 0.5 mg/l BA가 첨가된 SH배지 (3% sucrose, pH 5.8)에서 배양 5주 후 이루어졌다. 이와 같은 유식물체는 식물생장조절물질이 포함되지 않은 기본배지로 옮겼을 때 발근이 이루어졌다. 형질전환된 모상근 유래 식물체는 잎으로부터 직접 재분화된 식물체와 비교했을 때 잎이 오므라드는 경향을 보였고 줄기는 절간이 짧았으며, 뿌리는 가늘고 수량이 많은 특징을 보였다. 생체량 비교에서 형질전환식물체는 식물 하나의 무게가 1.28 g (생중량)으로 대조구인 잎 유래 재분화식물체 (0.53 g)에 비해 높았으며, 지표물질인 catalpol의 함량에 있어서도 차이를 보여주었다.

• 한국산학기술학회논문지
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• 제15권5호
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• pp.3231-3239
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• 2014

1970~1980년대의 급격한 경제개발과 도시의 발전 이후 현재에 들어 당시에 개발된 원도심지의 노후화와 기존기능이 빠르게 빠져나가는 도심공동화 현상이 발생하면서 근래에 도시재생이 도시계획 및 도시개발의 새로운 키워드로 등장하였다. 천안의 구도심인 명동지역(중앙동, 문성동)또한 공동화 현상이 급격하게 진행되면서 천안시에서는 재개발 및 활성화 사업을 진행하였지만 효과를 거두지 못하고 있다. 이에 본 연구에서는 이론적 고찰을 통해 도시재생 및 TOD개발 관련 사례를 분석해보고 명동지역의 현황분석을 통해 명동지역의 발전 및 공동화 이유와 기존 활성화 사업의 문제점을 분석하였다. 이를 바탕으로 명동지역의 새로운 활성화 계획을 제안하였다. 활성화 계획의 내용을 요약하면 다음과 같다. 1)명동지역의 경우 버스터미널과 천안역, 중앙시장까지 어우르는 도시흐름을 형성하는 하나의 결절점으로 고려되어야 하며, 2)민자역사유치, 지하상가와 연결된 쇼핑루트 개발, 통합 환승센터 및 이를 중심으로 한 마켓단지조성, 동남구청 부지의 문화센터 개발 및 예술공원 등의 개발이 서로 연계하여 이루어져야 한다.

• 아시안잔디학회지
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• 제11권4호
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• pp.311-320
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• 1997

This study was carried out to induce and maintain callus from 59 zoysiagrass lines, to know the effective disinfestation method for zoysiagrass stolon as explant and the difference in the response of callus induction among 59 lines, and to investigate the effect of medium, growth regulators, light, temperature, stolon part and internode position on callus induction and emhryogenic callus(E.C.) formation. The treatment of 0.lmg/L $HgCl_2$for 15 min resulted in no contamination and the highest callus induction(46.6%). Callus was induced from the 59 zoysiagrass lines. The callus growth of Z. japonica and Z. sinica was generally better than Z. matrella Ten cell lines whose callus and stolon grow fast in culture and in field, respectively were selected to he used for breeding. Callus induction was the most effective at 2.0mg /L of both 2, 4-D and picloram in MS medium. MS medium was the best for callus induction and growth while LS medium was the best for embryogenic callus and shoot formation. Callus induction and growth was better at 28, 31$^{\circ}C$. than 25$^{\circ}C$. and dark condition was better than light condition in MS me-dium containing 2mg/L 2,4-D. While callus induction was better with node part as explant than with internode part, callus growth and embryogenic callus formation was better with internode part. In 'Japonica 1', the first internode was the most effective in callus induction, but third internode was the best in '$M_2$ X $S_2$'.

• 원예과학기술지
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• 제31권3호
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• pp.352-358
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• 2013

포도 4배체 'Fujiminori'에 3배체 'Summer Black'을 인공교배하여 얻은 미숙배의 발아 및 식물체 분화를 목적으로 MS 배지에 $GA_3$와 Coconut water(CW)를 농도별로 첨가하여 배배양을 실시하였다. 'Fujiminori'에 'Summer Black'을 교배하여 64.3%의 미숙배를 얻을 수 있었고, 미숙배를 기내 배양한 결과 $GA_3$ 모든 처리구(0.01, 0.05, 0.25, $1.25mg{\cdot}L^{-1}$)에서는 95% 이상의 발아율을 나타내었으나, 15.8-31.6%만이 정상적인 식물체로 생장하였다. 또한 $GA_3$의 농도가 높아질수록 하배축은 신장되었고, 비대생장은 억제되었다. CW 모든 처리구(5, 10, 15, 20%) 중에 CW 10%와 CW 15% 처리구에서는 각각 68.4%와 66.7%로 많은 식물체를 획득할 수 있었다. 또한 줄기의 길이생장, 마디수, 발근수는 CW 10% 처리구에서, 뿌리길이는 CW 15% 처리구에서 양호한 결과를 나타내었다. 따라서 포도 4배체 'Fujiminori'와 3배체 'Summer Black'의 인공교배를 통해 얻어진 미숙배의 발아 및 생장을 위해서는 CW 10-15%를 첨가한 MS 배지를 이용한 배배양이 효과적이다.