• Genomics & Informatics
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• v.14 no.4
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• pp.241-254
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• 2016

Environmental microbes like Bordetella petrii has been established as a causative agent for various infectious diseases in human. Again, development of drug resistance in B. petrii challenged to combat against the infection. Identification of potential drug target and proposing a novel lead compound against the pathogen has a great aid and value. In this study, bioinformatics tools and technology have been applied to suggest a potential drug target by screening the proteome information of B. petrii DSM 12804 (accession No. PRJNA28135) from genome database of National Centre for Biotechnology information. In this regards, the inhibitory effect of nine natural compounds like ajoene (Allium sativum), allicin (A. sativum), cinnamaldehyde (Cinnamomum cassia), curcumin (Curcuma longa), gallotannin (active component of green tea and red wine), isoorientin (Anthopterus wardii), isovitexin (A. wardii), neral (Melissa officinalis), and vitexin (A. wardii) have been acknowledged with anti-bacterial properties and hence tested against identified drug target of B. petrii by implicating computational approach. The in silico studies revealed the hypothesis that lpxD could be a potential drug target and with recommendation of a strong inhibitory effect of selected natural compounds against infection caused due to B. petrii, would be further validated through in vitro experiments.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.11
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• pp.4935-4937
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• 2016

Objective: To compare dosimetric parameters of 3 dimensional conformal radiotherapy (3 DCRT) and intensity modulated radiotherapy (IMRT) in terms of target coverage and doses to organs at risk (OAR) in the management of rectal carcinoma. Methods: In this prospective study, conducted between August 2014 and March 2016, all patients underwent CT simulation along with a bladder protocol and target contouring according to the Radiation Therapy Oncology Group (RTOG) guidelines. Two plans were made for each patient (3 DCRT and IMRT) for comparison of target coverage and OAR. Result: A total of 43 patients were recruited into this study. While there were no significant differences in mean Planning Target Volume (PTV) D95% and mean PTV D98% between 3 DCRT and IMRT, mean PTV D2% and mean PTV D50% were significantly higher in 3 DCRT plans. Compared to IMRT, 3 DCRT resulted in significantly higher volumes of hot spots, lower volumes of cold spots, and higher doses to the entire OAR. Conclusion: This study demonstrated that IMRT achieves superior normal tissue avoidance (bladder and bowel) compared to 3 DCRT, with comparable target dose coverage.

• Journal of Korea Multimedia Society
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• v.21 no.6
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• pp.698-704
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• 2018

When the new ammunition is designed, it is necessary to confirm in advance how long the target distance is depends on the shape and weight of the designed ammunition. Therefore we can use commercial software such as PRODAS to predict the target distance in the design stage. This commercial software has aerodynamic data for various ammunition shape and calculates the target range by calculating the kinetic equations of the ammunition using the aerodynamic data most similar to the designed ammunition. The ammunition for predicting the target distance through software such as PRODAS is a non-guided ammunition that has no control after launch but the glide type ammunition is guided and control ammunition. So it is predicts the state of ammunition after the launch. A new type of simulator is needed to analyze the maximum range and to verify the onboard guided and control algorithm. The simulator constructed in this paper is an optimized simulator for glide type ammunition. Unlike unmanned aircraft and guided missiles. The rotation characteristics of the ammunition are considered and the navigation initialization algorithm is applied. The constructed simulator confirmed the performance by performing maximum range analysis of glide type ammunition.

• Journal of the Korea Institute of Military Science and Technology
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• v.23 no.1
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• pp.66-75
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• 2020

Enemy information has significant value when it comes to the process of military actions in battle field. Our Army now uses Battlefield Management Systems(BMSs) equipped in Ground Fighting Vehicles(GFVs) and we need to make research on what kind of role enemy information can play in such systems. Also, enemy information can be shared among GFVs and target information shall be extracted from it in view of KVMF scheme. Because KVMF becomes requisite standard in modern BMSs, we need to implement target information handling process in KVMF standard. In this article, we will focus on how target information and its sharing process can be managed efficiently without information conflicts. Also, situation map produced by it will be noted.

• Molecular & Cellular Toxicology
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• v.1 no.2
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• pp.92-98
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• 2005

DNA microarray analysis of gene expression in toxicogenomics typically requires relatively large amounts of total RNA. This limits the use of DNA microarray when the sample available is small. To confront this limitation, different methods of linear RNA amplification that generate antisense RNA (aRNA) have been optimized for microarray use. The target preparation strategy using amplified RNA in DNA microarray protocol can be divided into direct-incorporation labeling which resulted in cDNA targets (Cy-dye labeled cDNA from aRNA) and indirect-labeling which resulted in cRNA targets (i.e. Cy-dye labeled aRNA), respectively. However, despite the common use of amplified targets (cDNA or cRNA) from aRNAs, no systemic assessment for the use of amplified targets and bias in terms of hybridization performance has been reported. In this investigation, we have compared the hybridization performance of cRNA targets with cDNA targets from aRNA on a 10 K cDNA microarrays. Under optimized hybridization conditions, we found that 43% of outliers from cDNA technique and 86% from the outlier genes were reproducibly detected by both targets hybridization onto cDNA microarray. This suggests that the cRNA labeling method may have a reduced capacity for detecting the differential gene expression when compared to the cDNA target preparation. However, further validation of this discordant result should be pursued to determine which techniques possesses better accuracy in identifying truly differential genes.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.21 no.12
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• pp.1608-1614
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• 1997

The fluidic flow meter detects the gas flow rate based on the principle of fluidic oscillation instead of the conventional displacement method. It has many merits: wide rangeability, no moving mechanical parts and calibration insensitive to physical properties of fluids. The width of nozzle, size of oscillation chamber, size of target, width of outlet are tested to obtain the effects of jet oscillation on the fluidic flow meter. As the width of nozzle is too wide compared with the size of target, jet oscillation is unstable. The oscillation frequency decreases as the distance between the nozzle and target increases and also as the distance between target and outlet contraction increases. Two different vortexes exist in the front and the rear regions of the target, and they affect the oscillation frequency. The outlet contraction is very important, because the feedback flow is generated by the blocking of the flow. As the width of outlet increases, the jet oscillation frequency decreases. The linearity of this tested flow meter is quite good.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.5
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• pp.449-454
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• 2006

Here we present a sensitive DNA detection protocol using quantum dots (QDs) and magnetic beads (MBs) for large volume samples. In this study, QDs, conjugated with streptavidin, were used to produce fluorescent signals while magnetic beads (MBs) were used to isolate and concentrate the signals. The presence of target DNAs leads to the sandwich hybridization between the functionalized QDs, the target DNAs and the MBs. In fact, the QDs-MBs complex, which is bound using the target DNA, can be isolated and then concentrated. The binding of the QDs to the surface of the MBs was confirmed by confocal microscopy and Cd elemental analysis. It was found that the fluorescent intensity was proportional to concentration of the target DNA, while the presence of non-complementary DNA produced no significant fluorescent signal. In addition, the presence of low copies of target DNAs such as 0.5 pM in large volume samples up to 40mL was successfully detected by using a magnet-assisted concentration protocol which consequently results in the enhancement of the sensitivity more than 100-fold.

• Journal of Information Display
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• v.8 no.3
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• pp.5-10
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• 2007

Twin target sputtering (TTS) system with a configuration of vertically parallel facing Al targets and a substrate holder perpendicular to the Al target plane has been designed to realize a direct Al cathode sputtering on organic light emitting diodes (OLEDs). The TTS system has a linear twin target gun with ladder type magnet array for effective and uniform confinement of high density plasma. It is shown that OLEDs with Al cathode deposited by the TTS show a relatvely lower leakage current density $({\sim}1{\times}10^{-5}mA/cm^2)$ at reverse bias of -6V, compared to that ($1{\times}10^{-2}{\sim}10^{-3}$ $mA/cm^2$ at -6V) of OLEDs with Al cathodes grown by conventional DC magnetron sputtering. In addition, it was found that Al cathode films prepared by TTS were amorphous structure with nanocrystallines due to low substrate temperature. This demonstrates that there is no plasma damage caused by the bombardment of energetic particles. This indicates that the TTS system with ladder type magnet array could be useful plasma damage free deposition technique for direct Al cathode sputtering on OLEDs or flexible OLEDs.

• Korean Journal of Materials Research
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• v.24 no.7
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• pp.344-350
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• 2014

Metal films (i.e., Ti, Al and SUH310S) were prepared in a magnetron sputtering apparatus, and their cross-sectional structures were investigated using scanning electron microscopy. The apparatus used consisted of a cylindrical metal target which was electrically grounded, and two anode rings attached to the top and to the bottom of the target. A wire was placed along the center-line of the cylindrical target to provide a substrate. When the electrical potential of the substrate was varied, the metal-film formation rate depended on both the discharge voltage and the electrical potential of the substrate. As we made the magnetic field stronger, the plasma which appeared near the target collected on the plasma wall surface and thereby decreased the bias current. The bias current on the conducting wire was different from that for cation collection. The bias current decreased because the collection of cations decreased when we increased the magnetic-coil current. When the substrate was electrically isolated, the films deposited showed a slightly coarse columnar structure with thin voids between adjacent columns. In contrast, in the case of the grounded substrate, the deposited film did not show any clear columns but instead, showed a densely-packed granular structure. No peeling region was observed between the film and substrate, indicating good adhesion.

• Journal of the Institute of Electronics and Information Engineers
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• v.49 no.9
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• pp.362-369
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• 2012

Recently, a target detection based on a distributed sensor network has been much studied in active sonar. Zhou et al. proposed a target localization method using line fitting based on a distributed sensor network which consists of low complexity sensors that only report binary detection results. This method has three advantages relative to ML estimator. First, there is no need to estimate propagation model parameters. Second, the computation is simple. Third, it only use sensors with "detection", which implies less data to be collected by data processing center. However, this method has larger target localization error than the ML estimator. In this paper, a target localization method which modifies Zhou's method is proposed for reducing the localization error. The modified method shows the performance improvement that the target localization error is reduced by 40.7% to Zhou's method in the point of RMSE.