• 한국환경과학회지
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• 제17권11호
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• pp.1221-1226
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• 2008

A variety of fungal species are known to degrade cyanide through the action of cyanide hydratase, a specialized nitrilases which hydrolyze cyanide to formamide. This work is a report on two unknown and un-characterized members from Neurospora crassa and Aspergillus nidulans. Recombinant forms of three cyanide hydratases (CHT) originated from N. crassa, Gibberella zeae, and A. nidulans were prepared after their genes were cloned with N-terminal hexahistidine purification tags, expressed in E. coli and purified using immobilized metal affinity chromatography. These enzymes were compared according to their pH activity profiles, and kinetic parameters. Although all three were similar, the N. crassa CHT has the widest pH range of activity above 50% and highest turnover rate ($6.6{\times}10^8min^{-1}$) among them. The CHT of A. nidulans has the highest Km value of the three nitrilases evaluated in here. Expression of CHT in both N. crassa and A. nidulans were induced by the presence of KCN, regardless of any presence of nitrogen sources. These data can be used to determine optimal procedures for the enzyme uses in the remediation of cyanide-containing wastes.

• Journal of Microbiology and Biotechnology
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• 제12권3호
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• pp.522-525
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• 2002

A very rapid and efficient separation technique for cellular rhizobial cyclosophoraoses was developed based on fractional precipitation and partition chromatography. Cyclosophoraoses are known to function in the osmotic regulation and root nodule formation of legumes during the nitrogen fixation process. Cyclosophoraoses are produced as unbranched cyclic (1longrightarrow12)-${\beta}$-D-glucans in Agrobacterium or Rhizobium species. Recent research has shown that cyclosophoraoses can form inclusion complexation with various unstable or insoluble guest chemicals, thereby implying great potential for industrial application. Typical separation of pure cellular cyclosophoraoses has been so far carried out by several time-consuming steps, including size exclusion, anion exchange, and desalting liquid chromatographies, with a relatively poor recovery. However, the proposed method demonstrated that the successive application of fractional ethanol precipitation and one step of silica gel-based flash column chromatography was enough to simultaneously purify neutral or anionic forms of cyclosophoraoses. This novel technique is very rapid and provides a high recovery.

• 한국토양비료학회지
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• 제44권1호
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• pp.58-66
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• 2011

Arsenic pollution is a serious global concern which affects all life forms. Being a toxic metalloid, the continued search for appropriate technologies for its remediation is needed. Phytoremediation, the use of green plants, is not only a low cost but also an environmentally friendly approach for metal uptake and stabilization. However, its application is limited by slow plant growth which is further aggravated by the phytotoxic effect of the pollutant. Attempts to address these constraints were done by exploiting plant-microbe interactions which offers more advantages for phytoremediation. Several bacterial mechanisms that can increase the efficiency of phytoremediation of As are nitrogen fixation, phosphate solubilization, siderophore production, ACC deaminase activity and growth regulator production. Many have been reported for other metals, but few for arsenic. This mini-review attempts to present what has been done so far in exploring plants and their rhizosphere microbiota and some genetic manipulations to increase the efficiency of arsenic soil phytoremediation.

• 한국작물학회지
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• 제49권4호
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• pp.316-324
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• 2004

Sinorhizobium sp. strain MUS10 forms nitrogen-fixing stem nodules on Sesbania rostrata, a tropical green manure crop. In this study, the ultrastructural events associated with the formation of stem nodules were investigated. Sinorhizobium sp. strain MUS10 entered the host tissue through cracks created by the emerging adventitious root primordia and multiplied within the intercellular spaces. During early phases of infection, host cells adjacent to invading bacteria revealed cellular damage that is typical of hypersensitive reactions, while the cells at the inner cortex exhibited meristematic activity. Infection threads were numerous in S-day-old nodules and often were associated with the host cell wall. In several cases, more than one infection thread was found in individual cells. The junction at which the host cell walls converged was often enlarged due to fusion of intracellular branches of infection threads resulting in large infection pockets. The infection threads were made up of a homogeneous, amorphous matrix that enclosed the bacteria. Several finger-like projections were seen radiating from these enlarged infection threads and were delineated from the host cytoplasm by the plasma membrane. As in Azorhizobium caulinodans induced root nodules, the release of Sinorhizobia from the infection threads into the plant cells appears to be mediated by 'infection droplets'. A 15-day­old Sesbania stem nodule revealed typical ultrastructure features of a determinate nodule, containing several bacterioids within symbiosomes.

• 전자공학회논문지A
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• 제30A권1호
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• pp.31-43
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• 1993

Thermal behavior and $O_{2}$ plasma effects on residue and penetrated impurities formed by reactive ion etching (RIE) in CHF$_{3}$/C$_{2}$F$_{6}$ have been investigated using X-ray photoelectron spectroscopy (XPS) and secondary ion mass spectrometry (SIMS) techniques. Decomposition of polymer residue film begins between 200-300.deg. C, and above 400.deg. C carbon compound as graphite mainly forms by in-situ resistive heating. It reveals that thermal decomposition of residue can be completed by rapid thermal anneal above 800.deg. C under nitrogen atmosphere and out-diffusion of penetrated impurities is observed. The residue layer has been removed with $O_{2}$ plasma exposure of etched silicon and its chemical bonding states have been changed into F-O, C-O etc.. And $O_{2}$ plasma exposure results in the decrease of penetrated impurities.

• 지질공학
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• 제24권4호
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• pp.463-471
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• 2014

This paper considers the effect of rainfall on non-point source (NPS) pollutant loads. The impact of runoff on the occurrence of NPS pollutants was found to be influenced by rainfall amount, rainfall intensity, and the number of antecedent dry days (ADD), both independently and in combination. The close correlation ($R^2$ = 0.9920) between rainfall and runoff amounts was demonstrated at the study site (a flower farm) over the period between January 2011 and December 2013. The relationships among pollutant levels, runoff, and rainfall was not satisfactory results except for the Biochemical Oxygen Demand ($BOD_5$). The correlation coefficients between $BOD_5$, and both runoff and rainfall, were greater than 0.92. However, the relationships of other pollutants, such as Suspended Solid (SS), Chemical Oxygen Demand ($COD_{Mn}$), Total Nitrogen (TN), and Total Phosphorus (TP), with runoff and rainfall had correlation coefficients of less than 0.70. The roles of rainfall was different from rainfall categories on the occurrence of runoff. Instantaneous rainfall intensity was a principle factor on the occurrence of runoff following light rainfall events (total ${\leq}30mm$). For rainfall of intermediate intensity (total precipitation 31-50 mm), the combined effect of both average rainfall intensity and ADD was found to influence runoff generation. We conclude that the control of NPS pollutants with the reflection of the climate change that makes the remarkable effect of amounts and forms on the rainfall and runoff.

• 미생물학회지
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• 제27권3호
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• pp.285-290
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• 1989

한국에서 분리한 비유황 광합성 세균 Rhodospirillum rubrm KS-301 생장에 대하여 논 잡초제로 널리 쓰이는 butachlor(제품명 Machete)의 영향을 본 것이다. 실제 논에서 사용되고 있는 농노인 $10^{-3}$에서도 균의 생장은 이루어졌고 $10^{-2}$M의 농도에서 억제되어 다른 생물체보다 높은 저항력을 가지는 것으로 나타났다. 더욱이 pyruvate를 탄소원으로 하여 질소고정 상태로 배양했을 때 butachlor를 처리한 실험군에서 미약하나마 오히려 생장이 촉진되었다. (1%). 전자현미경으로 내부막조직을 관찰한 결과 Rhodospirillum rubrum의 전형적인 vesicular type이 tubular type으로 변형된 것으로 보아 제초제의 흡수가 간접 확인되었다. 또한 pH가 알칼리 조건일 때 질소고정상태에서 균생장에 대한 제초제의 저해력이 떨어지는 것으로 나타났다.

• Nuclear Engineering and Technology
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• 제5권4호
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• pp.286-290
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• 1973

염화카리움 단결정중 수산화물을 제거하고 이를 극미량의 음이온 불순물을 가하여 결정내에 점결함을 형성시키는 전 처리를 하였다. 이들 시료를 빛이 차단된 상태에서 액체질소 온도에서 원자로에 조사하였고 조사된 시료를 자외선으로 쪼인다음 4가지 산화상태 즉 -2, 0, +4, +6가의 $^{35}$ 를 화학적으로 상호분리하여 방사능을 계측하였다. 음이온 불순물로 처리된 시료는 순수한 결정에 비하여 낮은 산화상태 (-2가)의 $^{35}$ S 더 많이 생성되었고, 음이온 불순물로 처리된 시료 및 순수한 결정 모두를 자외선으로 쪼였을 때 30분까지는 -2가의 $^{35}$ S가 급격히 증가함을 알았다.

• 소성∙가공
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• 제21권1호
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• pp.36-41
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• 2012

Gas Assisted Injection Molding is a relatively new low-pressure injection molding technique that provides benefits such as reduced part warpage, excellent surface quality without shrink marks, greater design flexibility, etc. In the gas assisted injection molding process, the injected pressurized nitrogen gas flows through designed gas channels and forms hollow sections within the part. However, due to the characteristics of the gas, the design of the gas channels which are the paths for the injected gas is important in order to avoid defects such as gas blowout, fingering, etc. Therefore, in this study, the gas channel design for gas assisted injection molding of exterior display panels was conducted by examining the results of three CAE analyses. The designed gas channel was verified by conducting tryouts using a 450 ton injection molding machine with 3-stage pressure controlled gas kit. In addition, the hollow shapes which were formed by the gas with the installed gas channels were examined by examining the cross sections of the prototypes that were produced. As a result, it was found that exterior display panels can be produced without any defect by applying the gas assisted injection molding technique.

• Molecules and Cells
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• 제39권1호
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• pp.6-19
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• 2016

Redox signalling comprises the biology of molecular signal transduction mediated by reactive oxygen (or nitrogen) species. By specific and reversible oxidation of redoxsensitive cysteines, many biological processes sense and respond to signals from the intracellular redox environment. Redox signals are therefore important regulators of cellular homeostasis. Recently, it has become apparent that the cellular redox state oscillates in vivo and in vitro, with a period of about one day (circadian). Circadian timekeeping allows cells and organisms to adapt their biology to resonate with the 24-hour cycle of day/night. The importance of this innate biological timekeeping is illustrated by the association of clock disruption with the early onset of several diseases (e.g. type II diabetes, stroke and several forms of cancer). Circadian regulation of cellular redox balance suggests potentially two distinct roles for redox signalling in relation to the cellular clock: one where it is regulated by the clock, and one where it regulates the clock. Here, we introduce the concepts of redox signalling and cellular timekeeping, and then critically appraise the evidence for the reciprocal regulation between cellular redox state and the circadian clock. We conclude there is a substantial body of evidence supporting circadian regulation of cellular redox state, but that it would be premature to conclude that the converse is also true. We therefore propose some approaches that might yield more insight into redox control of cellular timekeeping.