• 한국균학회지
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• 제4권1호
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• pp.31-44
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• 1976

팽이버섯[Flammulina velutipes(Curt. ex Fr.) Sing.] 톱밥 재배(栽培)에 있어서 배지(培地)의 각종(各種) 영양급원(營養給源)의 첨가효과(添加效果) 및 배지(培地)의 물리적(物理的) 향상(向上)을 위(爲)한 방법(方法)을 구명(究明)하는 한편 재배과정중(栽培過程中) 일어나는 배지(培地)의 화학적(化學的) 성분(成分) 변화(變化)를 조사(調査)하여 팽이버섯의 영양요구(營養要求)에 대(對)한 기초자료(基礎資料)를 얻고자 일련(一連)의 시험(試驗)을 수행(遂行)한 바 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 포플러 톱밥에 미강(米糠), 밀기울 및 우분(牛糞)을 첨가비(添加比)를 달리하여 혼합(混合)한 배지(培地)에서 팽이버섯을 병재배(栽培)한 결과(結果), 포플러 톱밥 10에 미강(米糠) 3을 혼합한 처리구(處理區)에서 자실체(子實體) 수량(收量)이 84.4g/병(배지(培地)280g)으로서 가장 높았고 비교적(比較的) 수량(收量)이 높았던 포플러 톱밥 10에 미강(米糠) 3, 2 및 밀기울 3을 혼합한 처리구(處理區)에서 배지(培地)의 전질소(全窒素), 당류(糖類)와 가량(加量)의 함량이 높았다. 2. 톱밥 배지(培地)의 추출액(抽出液) 배지(培地)에서의 균사생장(菌絲生長)은 탄소(炭素) 및 질소(窒素) 등(等) 영양요소(營養要素)가 부족(不足)한 톱밥 단용구(單用區)나 우분첨가구(牛糞添加區)에서 극(極)히 저조(低調)하였으며, 톱밥 배지상(培地上)에서는 이들 처리구(處理區)에서 균사생장(菌絲生長) 속도(速度)는 빨랐으나 균사밀도(菌絲密度)는 현저(顯著)히 떨어졌다. 3. 가비중(假比重) 및 수분함량(水分含量)을 달리한 포플러 톱밥10+미강(米糠) 3 혼합배지(混合培地)에서 균사생장(菌絲生長)은 수분함량(水分含量) 72%, 가비중(假比重) 0.2에서 촉진(促進)되었으나 자실체(子實體) 수량(收量)은 가비중(假比重) 0.3, 수분함량(水分含量) 67%에서 가장 높았다. 4. 포플러 톱밥에 첨가(添加)되는 미강량(米糠量)이 증가(增加)됨에 따라 배지(培地)의 무게 감소율(減少率)은 커지며, 회분함량(灰分含量)도 증가(增加)하는 경향(傾向)을 보였고 종균(種菌) 접종(接種) 75일(日) 후(後) 무게 감소율(減少率)은 $17.8{\sim}28.8%$ 범위(範圍)였으며 회분(灰分)은 $10.1{\sim}13.2%$가 증가(增加)되었다. 5. 전재배기간중(全栽培期間中) cellulose는 $11{\sim}14%$, lignin은 $3{\sim}4%$의 절대량(絶對量)의 감소(減少)를 보여 포플러 톱밥에 첨가(添加)되는 미강량(米糠量)에 따른 큰 차이(差異)가 없었으며 가용성(可溶性) 당(糖)의 함량(含量)은 증가(增加)하는 경향(傾向)을 보였다. 6. 재배기간중(栽培期間中) 전질소(全窒素)는 시료(試料)에 대(對)한 함량(含量)은 증가(增加)하며 절대량(絶對量)은 감소(減少)하였으나 극(極)히 미소(微小)한 함량변화(含量變化)를 보였으며 유기태(有機態) 질소(窒素), 아미노태(態) 질소(窒素) 및 acid hydrolysable는 균사생장(菌絲生長) 및 자실체(字實體) 발생기간중(發生期間中)에 현저(顯著)히 감소(減少)되었다. 7. 질소원(窒素源)을 달리하는 배지(培地)를 사용(使用)한 액체배양(液體培養)에서 peptone, yeast extracts 등(等)의 유기태(有機態)가 첨가(添加)된 배지(培地)에서는 균사생장(菌絲生長)이 촉진(促進)된 반면(反面) 무기태(無機態) 질소원(窒素源)이 첨가(添加)된 Richard' solution과 Czapek-dox solution에서는 균사생장(菌絲生長)이 저조(低調)하였다. 또한 유기태(有機態) 질소원(窒素源)의 함량(含量)이 높은 배지(培地)에서 균사생장(菌絲生長)이 더욱 양호(良好)하였다.

• Applied Biological Chemistry
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• 제17권1호
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• pp.1-30
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• 1974

수도저위(水稻低位) 생산력(生産力)의 원인구명(原因究明)에 관(關)한 영양생리학적연구(營養生理적硏究)로 여러 가지 생육조건하(生育條件下)에서 품종별(品種別) 양분흡수(養分吸收) 및 동화기능(同化機能)과 양분(養分)의 체내(體內)에서의 이행양상(移行樣相)을 추구(追究)하는 한편 고수량지(高收量地) 벼와 저수량지(低收量地) 벼에 대(對)한 생육시기별(生育時期別)로 양분(養分)의 흡수(吸收) 체내무기양분(體內無機養分)과 수량(收量) 및 수량구성요소(收量構成要素)와의 관계(關係) 등(等)을 추구(追究)하여 저수량지(低收量地) 벼의 영양생리적(營養生理的) 저수요인(低收要因)을 밝히고저 본연구(本硏究)를 수행(遂行)하였던 바 그 결과(結果)를 요약(要約)하면 다음과같다. 1. 양분(養分)의 흡수력(吸收力)은 품종(品種)에 따라 차이(差異)가 있으며 인산(燐酸) 및 가리(加里)의 흡수력(吸收力)이 강(强)한 품종(品種)은 구사부에, 진흥(振興)이며 낮은 품종(品種)은 팔달(八達)이었다. 2. 근(根)의 ${\alpha}-naphthylamine$의 산화력(酸化力)과 인산(燐酸)의 흡수력(吸收力)과는 유의적(有意的)인 상관관계(相關關係)가 있으며 산화력(酸化力)이 높으면 인산(燐酸)의 흡수(吸收)가 증대(增大)되었바. 3, 탄소동화력( 炭素同化力)은 품종(品種)에 따라 차이(差異)가 있으며 노인도(老人稻), 구대내조욱(九大耐潮組) 3호(號), 수원(水原)82호(號), 진흥(振興) 등은 강(强)하고 대구조(大脚租), 관옥(關玉), 육우(陸羽) 132호(號)가 약(弱)하였다. 4, 질소(窒素)의 증시(增施)는 소비(少肥)에 비(比)하여 동화량(同化量)은 증가(增加) 되나 등화산물(同化産物)의 이삭으로의 이전(移轉)은 적었다5. 정상(正常) 생육(生育)한 벼에 비(比)하여 인산(燐酸), 가리(加里) 및 마그네슘이 결핍(缺達)된 벼는 전탄소동화량이(全炭素同化量)이 정상(正常) 벼 100에 비(比)하여 40이하(以下)로 현저(顯著)히 낮으며 그 순서(順序)는 Mg>P>K이고, 동화물(同化物)의 이전(移轉)에 장해(障害)가 큰 것은 K>Mg>P의 순(順)이었다. 6. 고수량지(高收量地) 벼는 저수량지(低收量地) 벼에 비(比)하여 총건물(總乾物) 생산량(生産量)이 현저(顯著)하게 많고 특(特)히 유수형성기(幼穗形成期) 이후(以後)에 증가량(增加量)이 더욱 현저(顯著)하였다. 7. 고수량지(高收量地) 벼는 출수기(出穗期)에 옆면적(葉面積)이 최고(最高)에 달(達)하였다가 그 이후(以後) 완만(緩慢)하게 줄어지나 저수량지(低收量地) 벼는 출수이전(出穗以前)에 엽면적(葉面積)이 최고(最高)에 달(達)하였다가 출수이후(出穗以後)에 급격(急激)히 감소(減少)되며 그 원인(原因)은 하엽(下葉)의 조기고사(早期枯死)에 기인(起因)되었다. 8. 고수량지(高收量地) 벼는 저수량지(低收量地) 벼에 비(比)하여 생육초기(生育初期)에는 투과광율(透過光率)이 높고 생육후기(生育後期)에 낮았으나 투과광율대(透過光率對) 엽면적비(葉面積比)는 고수량지(高收量地) 벼에서 높았다. 9. 순동화량(純同化量)은 저수량지(低收量地) 벼에 비(比)하여 고수량지(高收量地)벼에서 많았으며 이는 또한 엽면적(葉面積)이 증가(增加)됨에 따라 감소(減少)되었다. .10. 고수량지(高收量地) 벼는 저수량지(低收量地) 벼에 비(比)하여 유수형성기(幼穗形成期) 이후(以後) 체내질소(體內窒素), 가리(加里) 및 규산(珪酸)의 함유율(含有率)이 높으면서 $K_2O/N$, $SiO_2/N$ 비(比)가 높았다.11. 저수량지(低收量地) 벼는 고수량지(高收量地) 벼에 비(比)하여 출수기(出穗期)에 보유(保有)된 질소(窒素), 인산(燐酸), 가리(加里) 및 마그네슘등이 출수이후(出穗以後) 이삭으로의 이행율(移行率)이 높았다. 이는 저수량지(低收量地) 벼가 생육후기(生育後期)에 양분(養分)의 흡술량(吸收量)이 부족(不足)하여 체내(體內) 저장양분(貯藏養分)이 재이동(再移動)되기 때문이며 따라서 하엽(下葉)은 양분부족(養分不足)으로 조길고사(早期桔死)가 많아지는 것으로추정(推定)되었다. 12. 고수량지(高收量地) 벼는 질소흡수(窒素吸收)가 전생육기간(全生育期間)을 통(通)하여 완만(緩慢)하며 출수이후(出穗以後)에도 흡수량(吸收量)이 많으나 저수량지(低收量總) 벼는 출수이전(出穗以前)에 많고 출수이후(出濾以後)는 거의 흡수(吸收)되지 않았다. 13. 출수기(出穗期)의 체내(體內) 질소(窒素), 가리(加里) 함유율(含有率)과 수량(收豊)과는 현저(顯著)한 정(正)의 상관(相關)이 성립(成立)되였으며 수확기(收獲期)의 가리(加里), 규산함유율(珪酸含有率) 그리고 $K_2O/N$, $SiO_2/N$과 수량(收量)과도 현저(顯著)한 정(正)의 상관관계(相關關係)가 성립(成立)되었다. 14. 출수기(出穗期)에 보유(保有)된 전분(澱粉)이 이삭으로의 이전(移轉)은 고수량지(高收量地) 벼가 약(約) 10%인데 반(反)하여 저수량지(低收量地) 벼는 약(約) 40%로 많았다. 따라서 저수량지(高收量地) 벼는 출수(出穗) 이후(以後) 동화의존도(同化依存度)가 높았다. 15. 고수량지(高收量地) 벼는 저수량지(低收量地) 벼에 비(比)하여 질소(窒素), 인산(燐酸), 가리(加里), 규산(珪酸) 및 망간의 흡수량(吸收量)이 현저(顯著)하게 많았고 생육시기별(生育時期別) 흡수량(吸收量)의 차이(差異)는 유수형성기(幼穗形成期) 전후(前後)에 현저(顯著)하였다. 16. 수확기(收穫期)의 질소(窒素), 인산(燐酸), 가리(加里), 석탄(石炭), 마그네슘, 규산(珪酸) 및 망간의 총흡수량(總吸收量)과 수량(收量)과는 고도(高度)의 정(正)의 상관관계(相關關係)가 인정(認定)되었다. 17. 수확기(收穫期)에 질소(窒素), 인산(燐酸), 가리(加里), 석탄(石炭), 마그네슘 및 규산(珪酸)의 흡수량(吸收量)과 수당입수간(穗當粒熱間)에, 질소(窒素) 및 가리(加里)의 흡수량(吸收量)과 수수(穗數)와는 유의적(有意的)인 정(正)의 상관관계(相關關係)가 성립(成立)되었다.

• 멤브레인
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• 제14권4호
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• pp.289-297
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• 2004

유기물과 질소를 동시에 제거하기 위하여 연속회분식으로 운전한 MBR (membrane bioreactor)시스템에서 미생물농도와 슬러지 부하량이 막오염과 미생물 활성에 미치는 영향을 살펴보았다. 막오염은 MLSS (mixed liquor suspended solid) 농도 증가에 따라 조금씩 증가하는 경향을 보였고, 그 효과는 비포기 조건보다 포기 조건에는 좀더 두드러지게 나타났다. MLSS 농도는 막오염에 직접적인 커다란 영향을 주지는 않으나, 지나치게 높은 MLSS에서 유도되는 낮은 슬러지 부하에서는 막오염이 크게 증가하는 현상이 발견되었고, 이러한 조건에서는 포기에 의한 막 세척 효과도 크게 줄어들었다. 미생물의 개별 활성도는 슬러지 부하가 감소할수록 지속적으로 감소하는 경향을 나타내었다 반응조 전체 활성도 또한 17,000 mg/L 이상의 높은 MLSS로부터 유도되는 낮은 슬러지 부하율에서는 높은 미생물 농도에도 불구하고 오히려 감소했는데 이는 기질 부족으로 인한 경쟁으로 활성도가 떨어지고, 용액의 점성 증가로 인해 산소 전달율이 저하되었기 때문이다.

• Bulletin of the Korean Chemical Society
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• 제28권10호
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• pp.1751-1755
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• 2007

The interaction of ammonium hydroxide (NH4OH) with zircaloy-4 (Zry-4) was investigated using X-ray photoelectron spectroscopy (XPS) and Auger electron spectroscopy (AES) methods. In order to study the surface chemistry of NH4OH/Zry-4 system, the binding energies of N1s, O1s and Zr3d electrons were monitored. The N1s peak intensity was remarkably increased by following cycles of Ar+ sputtering of NH4OH dosed Zry-4 surface at room temperature. Because the nitrogen stayed under the subsurface region was diffused out onto the Zry-4 surface after oxygen concentration was decreased. These could be occurred after the surface oxygen was diffused into the bulk or desorbed out from the surface until Ar+ fluence was 6.0 × 1016 Ar+/cm2 then the surface was relatively atomic deficient state. The O1s peak intensity was decreased by stepwise Ar+ sputtering. After many cycles of Ar+ sputtering, the peak intensities of Zr3d peaks did not change much but the shape of the peak clearly did change. This implies that the oxidation state of zirconium was changed during stepwise Ar+ sputtering of NH4OH/Zry-4. The Zr3d peak intensity of zirconium nitride (ZrNx) increased as the intensity of N1s (from zirconium nitride) increased but the Zr3d peak intensity of zirconium oxide (ZrOx) decreased due to the depopulation of the oxygen species on the surface region. We also observed that the peak intensity of Zr4+ was nearly same after Ar+ sputtering processes but the peak intensity of metallic zirconium increased compared to that of before the sputtering process was performed.

• Journal of Applied Biological Chemistry
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• 제51권3호
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• pp.102-110
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• 2008

Complementation assay of the urea uptake-defective yeast mutants led to the identification of the Arabidopsis AtTIP4;1 gene encoding the aquaporin. However, its physiological functions still remain elusive. In the present study, histochemical and genetic analyses were performed to understand the physiological roles of AtTIP4;1 in urea uptake. The AtTIP4;1 product was detectible in the roots, but not in the leaves, the stem, and the flower. Its promoter allowed the expression of the $\beta$-glucuronidase reporter gene in the roots and the apical meristem in Arabidopsis. The AtTIP4;1 products were induced under nitrogen-deficient conditions. To investigate the role of the tonoplast intrinsic protein in urea transport and developments, Arabidopsis with the loss- and the gain-of-function mutations by T-DNA insertion in AtTIP4;1 and 35S promoter-mediated overexpression of AtTIP4;1 were identified, respectively. The transfer DNA insertion and the AtTIP4;1-overexpressed plants showed normal growth and development under normal or abiotic stress growth conditions. The urea-uptake studies using $^{14}C$-labeled urea revealed higher accumulation of urea in the AtTIP4;1-overexpressed plants. These results provide evidence that overexpression of AtTIP4;1 leads to the increase in the urea-uptake rate in plants without detectable defects to the growth and development.

• 한국환경과학회지
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• 제3권3호
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• pp.185-195
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• 1994

Masan bay is one of the polluted enclosed bays, which has red tides problem and the formation of oxygen deficient water in the bottom layer. Most important factors that cause eutrophication and red tide is nutrient materials containing nitrogen and phosphorus which stem from terrestrial sources and nutrients released from sediment. Therefore, to improve of water quality, reduction of these nutrient loads should be indispensible. At this study, the three-dimensional numerical hydrodynamic and eutrophication model, which were developed by Institute for Resources and Environment of Japan, were applied to analyze the processes affecting the phytoplankton production and also to evaluate the effect of water quality improvement plans on phytoplankton production. In field sorvey, the range of concentrations of chlorophyll-a at surface area was found to be 29.17 - 212.5mg/m3, which were exceeding eutrophication criteria. The constant currents defined by integrating the simulated tidal currents over 1 tidal cycle showed the counterclockwise eddies in the southern part of Budo. The general directions of constant currents were found to be southward at surface and northward at bottom over all the bay. The eutrophication model was calibrated with the data surveyed in the field area in June, 1993. The calculated results are in fairly good agreement with values within relative error of 30%. The pollutant load from the sources such as the input from terrestrial release from the sediment was reduced by the rate of 50, 70, 90, 98% to effect of phytoplankton production. Phytoplankton production was reduced to of the 90% reduction of the input loads from terrestrial sources and 8% in 90% reduction of the load from sediment.

• 대한수의학회지
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• 제41권3호
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• pp.407-412
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• 2001

Milk data may be increasingly used as indicators of the protein-energy balance and actual farm feeding practices. It was related to milk production, nutritional and reproductive disorders. The purpose of this study was to investigate the relationship between level of fat, protein or milk urea nitrogen (MUN) from the first test within 35 days in milk and displaced abomasum (DA) in a large dairy herd with high yielding Holstein cows. Milk data from forty-five DA cases were compared to those from 90 healthy cows. Higher odds of DA diagnosis was found with higher 5.0% milk fat, lower 3.0% milk protein. Therefore, cows with a fat to protein ratio of>1.5 had higher risks for DA. Also, incidence rates of DA was higher in the cows which the level of MUN was lower than 12.0 mg/dl or higher than 25.0 mg/dl relative to healthy cows. These results indicate that cows diagnosed with DA were energy deficient prior to DA diagnosis. We conclude that level of fat, protein or MUN serve as a monitoring tool of protein and energy nutritional balance in early lactation cows and also as a significant predictor of risk for DA.

• 한국토양비료학회지
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• 제45권6호
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• pp.910-919
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• 2012

The role of inorganic nitrogen assimilation in the production of amino acids, organic acids and soluble sugars is one of the most important biochemical processes in plants, and, in order to achieve normally, nitrate uptake and assimilation is essential. For this reason, the characterization of nitrate assimilation and metabolite composition from leaves, roots and xylem sap of tomato (Solanum lycopersicum) was investigated under different nitrate levels in media. Tomato plants were grown hydroponically in liquid culture under five different nitrate regimes: deficient (0.25 and 0.75 mM $NO_3{^-}$), normal (2.5 mM $NO_3{^-}$) and excessive (5.0 and 10.0 mM $NO_3{^-}$). All samples, leaves, roots and xylem sap, were collected after 7 and 14 days after treatment. The levels of amino acids, soluble sugars and organic acids were significantly decreased by N-deficiency whereas, interestingly, they remained higher in xylem sap as compared with N-normal and -surplus. The N-excessive condition did not exert any significant changes in metabolites composition, and thus their levels were similar with N-normal. The gene expression and enzyme activity of nitrate reductase (NR), nitrite reductase (NIR) and glutamine synthetase (GS) were greatly influenced by nitrate. The data presented here suggest that metabolites, as a signal messenger, existed in xylem sap seem to play a crucial role to acquire nitrate, and, in addition, an increase in ${\alpha}$-ketoglutarate pathway-derived amino acids under N-deficiency may help to better understand plant C/N metabolism.

• Journal of Microbiology and Biotechnology
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• 제30권6호
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• pp.839-847
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• 2020

In the present study, an anaerobic microbial consortium for the degradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) was selectively enriched with the co-addition of RDX and starch under nitrogen-deficient conditions. Microbial growth and anaerobic RDX biodegradation were effectively enhanced by the co-addition of RDX and starch, which resulted in increased RDX biotransformation to nitroso derivatives at a greater specific degradation rate than those for previously reported anaerobic RDX-degrading bacteria (isolates). The accumulation of the most toxic RDX degradation intermediate (MNX [hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine]) was significantly reduced by starch addition, suggesting improved RDX detoxification by the co-addition of RDX and starch. The subsequent MiSeq sequencing that targeted the bacterial 16S rRNA gene revealed that the Sporolactobacillus, Clostridium, and Paenibacillus populations were involved in the enhanced anaerobic RDX degradation. These results suggest that these three bacterial populations are important for anaerobic RDX degradation and detoxification. The findings from this work imply that the Sporolactobacillus, Clostridium, and Paenibacillus dominant microbial consortium may be valuable for the development of bioremediation resources for RDX-contaminated environments.

• Journal of Nutrition and Health
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• 제15권1호
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• pp.70-79
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• 1982

Using dietary, anthropometric, and biochemical methods of evaluation, a nutritional survey was made of 81 seven-and eight-year-old children from two schools with different socioeconomic backgrounds in Daejon city, during the October of 1980. All the children were tall and heavy in comparison with 1976 Korean standard. School A children with relatively high socioeconomic levels showed higher value in length and lower value in weight than school B children with relatively low socioeconomic levels. Mean hemoglobin values were 13.3 (school A) and 12.8 (school B) g/100 ml and hematocrits were 39.7 and 37.9%, respectively. Anemic children were not many in both schools ( A ; 0-25%, B ; 12.5-15.0% ). Urea nitrogen/creatinine ratios were 9.2 (A) and 7.8 (B). The intake of animal foods was higher in school A owing to the higher intake of milks. The intakes of all nutrients except energy and calcium were comparable to or higher than Korean recommend dietary allowances (KRDA). Riboflavin intake wae deficient in school B only. Low socioeconomic school B children had significantly lower intakes of fats, calcium, and riboflavin than school A children. Energy intakes were low as 75.9 (A) and 83.2 (B) % of KRDA but their composition of carbohydrates, fats, and proteins were satisfactory in both schools. More than one-third of protein intake was animalorigin in both schools (A ; 1.40/3, B ; 1.10/3) and calcium intake was very low as 59.0%(A) and 45.8%(B). Education levels of parents, esp. mothers, were positively correlated with Kaup indice (in school A & B) and some nutrients intakes (in school A). In school B, number of siblings and birth order showed negative correlations with some nutrient intakes.