• 제목/요약/키워드: Nitrogen Fractions

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Change in Nitrogen Fractions and Ruminal Nitrogen Degradability of Orchardgrass and Alfalfa during the Ensiling Process and the Subsequent Effects on Nitrogen Utilization by Sheep

  • Nguyen, H.V.;Kawai, M.;Takahashi, J.;Matsuoka, S.
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권11호
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    • pp.1524-1528
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    • 2004
  • In order to determine the extent of change in nitrogen fractions and in vitro ruminal degradability of forage protein during ensilage and the influence on nitrogen utilization by sheep, orchardgrass (Dactylis glomerata L.) and alfalfa (Medicago sativa L.) were ensiled in separate 120 L silos for 5, 21 and 56 days. With respect to nitrogen fractions, fraction 1 (buffer solution soluble nitrogen), fraction 2 (buffer solution insoluble nitrogen-neutral detergent insoluble nitrogen), fraction 3 (neutral detergent insoluble nitrogen-acid detergent insoluble nitrogen), and fraction 4 (acid detergent insoluble nitrogen) were determined. Fractions 1 and 2 accounted for more than 80% of total nitrogen in orchardgrass and 90% of that in alfalfa. The proportion of fraction 1 in orchardgrass increased from 33.0% at day 0 to 52.0% after day 56 of ensiling. In the case of alfalfa silage it was 41.7% and 62.9%, respectively. Seventy percent of this increase occurred within the first 5 days of ensiling. A similar change of in vitro ruminal degradability of total nitrogen was also observed in both forages. Nitrogen retention in sheep tended to decrease as the length of ensiling increased, with a significantly positive correlation between urinary nitrogen and fraction 1, and in vitro ruminal degradability of total nitrogen.

미생물 호흡률 측정에 의한 관거시스템 유형별 하수의 기질 분율 평가 (Assessment of the Organic and Nitrogen Fractions in the Sewage of the Different Sewer Network Types by Respirometric Method)

  • 박종부;허형우;강호;장성언
    • 대한환경공학회지
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    • 제31권8호
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    • pp.649-654
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    • 2009
  • 산소소비속도 측정에 의한 미생물 호흡률 분석방법에 의해 하수의 유기물질과 미생물 분율을 평가하였다. 하수의 유기물과 미생물 분율은 생물학적 공정의 모델링을 위한 중요한 기초자료이다. 본 연구에서 하수의 유기물 분율을 측정한 결과, 미생물에 의해 분해가 빠른 유기물, 분해가 느린 유기물, 분해되지 않는 용존성 유기물, 분해되지 않는 고형 유기물과 종속영양미생물의 분율은 각각 26.6%, 41.5%, 8.5%, 14.7% 및 8.7%였다. 또한, 질소 분율을 측정한 결과, 질산성 질소, 암모니아성 질소, 용존성 비분해 유기성 질소, 용존성 분해 유기성 질소 및 분해가 느린 유기성 질소의 분율은 각각 약 3.7%, 64.9%, 4.7%, 9.4% 및 17.4%였다.

동대만과 오지리 연안에 서식하는 해초(Zostera marina)내 질소함유율의 계절적 변화 (Seasonal Nitrogen Dynamics of Zostera marina Inhabited in Dongdae Bay and Ojiri)

  • 김민섭;이성미;신경훈
    • 환경생물
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    • 제24권2호
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    • pp.186-194
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    • 2006
  • Nitrogen dynamics of Seagrass Zostera marina were investigated in both Dongdae Bay and Ojiri from March to August, 2004. All seagrass samples were separated into four fractions such as leaves (new and adult), sheath and rhizome in order to understand temporal variations of nitrogen content in different fractions of Zostera marina. There are temporal variations of shoot production rates and total nitrogen contents in their different fractions at both study areas. Leaf production were almost 4 to 5 fold higher in summer than in winter. The irradiance is the primary factor controlling the leaf production of Zostera marina in both sites although water temperature also influence its productivity. Nitrogen contents of leaves were overall low in summer than in winter, but nitrogen content of rhizome increased during the summer season. In addition, nitrogen contents of new leaves were mostly higher than adult leaves in spite of lower nitrogen content of new and adult leaves in high productivity period. This result suggests that Zostera marina seems to have significant translocation ability of nitrogen in a shoot. The nitrogen content of leaf tissue may reflect nutritional nitrogen availability.

목초 사일리지 급여 시 보리와 채종박 보충급여에 의한 제 3위 소화액내 Soluble Non-ammonia Nitrogen Fraction의 Flow 패턴 변화 (Diurnal Patterns in the Flow of Escapable Soluble Non-Ammonia Nitrogen Fractions in Omasal Digesta as Influenced by Barley and Rapeseed Meal Supplementation in Cows Fed Grass Silage Based Diet)

  • 최창원
    • Journal of Animal Science and Technology
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    • 제49권3호
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    • pp.341-350
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    • 2007
  • 본 연구는 목초 사일리지를 기초사료로 급여한 젖소에 있어서 보리와 유채박 급여가 제 3위 소화액 내 soluble non-ammonia nitrogen fractions(SNAN fractions; 아미노산, 펩티드, 용해성 단백질 및 total SNAN)의 flow 패턴변화를 측정하기 위하여 실시하였다. 반추위 캐뉼라가 장착된 4마리 젖소는 4×4 라틴방각법에 의해 기초사료로 grass-red clover silage를 자유급여하여 사일리지 단독급여구(GS), 기초사료 + 보리6.0kg/d 급여구, 기초사료 + 유채박2.1kg/d 급여구 및 기초사료 + 보리6.0kg/d + 유채박2.1kg/d 급여구로 배치하였다. 제 3위 소화액은 제 3위 소화액 채취기구를 이용하여 사료급여 후 1.5h 간격으로 채취하였고, 그 소화액 내 SNAN fractions 정량은 ninhydrin assay를 이용하여 분석하였다. 보충사료 급여는 GS구에 비해 비록 통계학적 유의성은 없었지만 total SNAN flow를 증가시켰다. 제 3위 소화액 내 펩티드 flow 패턴은 12시간 feeding cycle 내내 SNAN fractions 중 양적으로 가장 높았으며, GS구에 비해 보충사료급여구에서 높은 펩티드 flow 패턴을 보였다. GS구를 제외한 보충사료처리구간에는 SNAN fractions flow 패턴 차이가 없었다. 15N을 이용하여 분석된 사료유래 SNAN fractions flow 패턴에서는 보충사료급여구가 GS구에 비해 높게 나타났다. 이상의 결과 제 3위로 유입되는 펩티드 flow는 전체 SNAN flow 중 양적으로 가장 중요한 N 이며, 보충사료 급여 시 그 펩티드 flow의 증가 가능성을 시사한다.

시유의 질소분획물 함량과 유청단백질 변성정도 (Contents of Nitrogen Fractions and the Degree of Whey Protein Denaturation in Market Milks)

  • 박영희;홍윤호
    • 한국식품영양과학회지
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    • 제22권2호
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    • pp.161-164
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    • 1993
  • 본 연구에서는 Kjeldahl 방법으로 시유의 질소분획물의 함량과 유청단백질 변성정도를 측정함으로써 이들이 시유의 열처리 정도를 비교할 수 있는 검색의 지표로서 타당하는지 알아보았다. 각 열처리법에 따른 질소분획물들의 함량을 보면, 원유에서는 시료 100g당 총질소가 431.3mg, 케이신질소가 341.0mg, 비케이신질소가 90.3mg, 이 중에서 비단백태질소가 31.6mg그리고 유청단백질소는 58.8mg을 보인 반면, 시유인 저온살균유, 고온순간살균유, 초고온처 리유의 질소분획물들의 함량이 다르게 나타났다. 유청단백질 변성정도는 저온살균유가 26.7%, 고온순간살균유가 32.9%, 초고온살균유가 60.7%, 그리고 초고온멸균유가 38.4%를 보여 열처리 정도가 높아질수록 변성정도도 높게 나타남으로써 열처리법에 따라 유청단백질의 변성정도의 구분이 뚜렷하였다.

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Change in Nitrogen Fractions and Ruminal Nitrogen Degradability of Orchardgrass Ensiled at Various Moisture Contents and the Subsequent Effects on Nitrogen Utilization by Sheep

  • Nguyen, H.V.;Kawai, M.;Takahashi, J.;Matsuoka, S.
    • Asian-Australasian Journal of Animal Sciences
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    • 제18권9호
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    • pp.1267-1272
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    • 2005
  • The effect of various moisture contents of fresh forage on the change in nitrogen (N) fractions, in vitro ruminal N degradability, and the subsequent N utilization of silage in sheep were evaluated. Orchardgrass (Dactylis glomerata L.) with high (HM, 76%), medium (MM, 65%) and low (LM, 40%) moisture contents were ensiled into silos of 120 L capacity for 120 days. A nitrogen balance trial was conducted using a 4${\times}$4 Latin square design consisting of four dietary treatments (i.e. fresh forage, HM, MM and LM silages) and four wethers. With respect to N fractions, fraction 1 (buffer solution soluble N), fraction 2 (buffer solution insoluble N-neutral detergent insoluble N), fraction 3 (neutral detergent insoluble N-acid detergent insoluble N), and fraction 4 (acid detergent insoluble N) were determined. The proportion of fraction 1 in silages tended to decrease, while the in vitro ruminal degradability of insoluble N increased (p<0.05) with lower moisture contents at ensiling. Consequently, nitrogen utilization in sheep tended to improve as the moisture content of ensiled grass was decreased, with a negative correlation (p<0.01) between urinary N and the in vitro ruminal degradability of insoluble N. The averaged N retentions for HM, MM, and LM silage treatments were 59, 73 and 79% of that for fresh forage, respectively.

우리나라 서남해안 간척지 및 간석지 토양의 화학적 특성 (III) -질소의 형태별 함량 분포- (Chemical Properties in the Soils of Reclaimed and Natural Tidelands of Southwest Coastal Area of Korea (III) - Distribution of Nitrogens Fractions -)

  • 조재영;구자웅;손재권
    • 한국농공학회논문집
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    • 제48권3호
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    • pp.3-8
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    • 2006
  • The fractions of nitrogen were investigated in the soils of reclaimed and natural tidelands of southwest coastal area of Korea. The content of total-N varied to ranged form 619 to 5,534 mg/kg dry weight with a mean value of 1,857 mg/kg. The order of different fractions of nitrogen was as follows: Acid non-extractable-N > uncounted-N > acid extractable-N > ammonia-N > nitrate-N. The content of ammonia-N, acid extractable-N, and acid non-extractable-N showed highly negative correlations with pH. The content of acid extractable-N and ammonia-N showed highly positive correlation with clay content.

Differentiation in Nitrogen-Converting Activity and Microbial Community Structure between Granular Size Fractions in a Continuous Autotrophic Nitrogen Removal Reactor

  • Qian, Feiyue;Chen, Xi;Wang, Jianfang;Shen, Yaoliang;Gao, Junjun;Mei, Juan
    • Journal of Microbiology and Biotechnology
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    • 제27권10호
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    • pp.1798-1807
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    • 2017
  • The differentiations in nitrogen-converting activity and microbial community structure between granular size fractions in a continuous completely autotrophic nitrogen removal over nitrite (CANON) reactor, having a superior specific nitrogen removal rate of $0.24g/(g\;VSS{\cdot}h)$, were investigated by batch tests and high-throughput pyrosequencing analysis, respectively. Results revealed that a high dissolved oxygen concentration (>1.8 mg/l) could result in efficient nitrite accumulation with small granules (0.2-0.6 mm in diameter), because aerobic ammonium-oxidizing bacteria (genus Nitrosomonas) predominated therein. Meanwhile, intermediate size granules (1.4-2.0 mm in diameter) showed the highest nitrogen removal activity of $40.4mg/(g\;VSS{\cdot}h)$ under sufficient oxygen supply, corresponding to the relative abundance ratio of aerobic to anaerobic ammonium-oxidizing bacteria (genus Candidatus Kuenenia) of 5.7. Additionally, a dual substrate competition for oxygen and nitrite would be considered as the main mechanism for repression of nitrite-oxidizing bacteria, and the few Nitrospira spp. did not remarkably affect the overall performance of the reactor. Because all the granular size fractions could accomplish the CANON process independently under oxygen limiting conditions, maintaining a diversity of granular size would facilitate the stability of the suspended growth CANON system.

예취주기가 White Clover의 질소고정과 질소 및 탄수화물 분배에 미치는 영향 ($N_2$Fixation and Partitioning of Nitrogen and Carbohydrate in White Clover as Affected by Defoliation Interval)

  • 강진호
    • 한국작물학회지
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    • 제40권1호
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    • pp.26-32
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    • 1995
  • White clover는 많은 장점에도 불구하고 계속방목에 대한 적응력이 낮아 존속연한이 짧은 것이 결점이다. 따라서 본 시험은 white clover 혼파초지관이에 필요한 정보를 제공하기 위하여 예취주기의 장단(7일, 14일, 28일)이 28일의 재생기간 동안 잎의 크기가 다른 white clover 품종(Osceola, Huia, S184)의 질소고정, 각부위(잎과 엽병, 포복경 및 뿌리)별 total nonstructural carbohyd-rate(TNC) 및 질소의 분배에 미치는 영향을 조사하기 위하여 온실에서 pot 시험으로 실시한 바 그 결과를 요약하면 다음과 같다. 1. TNC 함량은 포복경과 뿌리에서 예취주기를 7일에서 28일로 늘임으로써 증가하였고 대엽종인 Osceola에서 가장 높은 함량을 보인 반면, 중엽종인 Huia와 소엽종인 5184은 각부위간분배가 다른 것으로 나타났다. 2. 질소함양은 전부위에서 이상의 포복경과 뿌리의 TNC 함량과 유사한 반응을 보였다. 3. TNC 함량은 뿌리보다는 잎과 엽병 또는 포복경에서 많은 반면, 질소함량은 엽병, 뿌리, 포복경의 순으로 감소하였다. 4. 예취로 인하여 전부위의 TNC 및 질소 함량은 감소하였으나, 재생기간이 길어짐으로써 증가하는 경향이었다. 또한 예취주기에 따라 재생기간 중 각부위간 TNC 및 질소 분배에는 차이가 있는 것으로 조사되었다. 5. 질소고정은 예취주기가 7일에서 28일로 증가함으로써 증가하고 대엽종일수록 큰 것으로 나타났으며, 예취 3일후까지는 감소하다가 그 이후에는 증가하였다.

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Serial Particle Size Fractionation and Water Quality in a Recirculating Aquaculture System for Eel

  • Lee, Jin-Hwan
    • Fisheries and Aquatic Sciences
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    • 제13권2호
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    • pp.133-139
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    • 2010
  • The effects of suspended solids size on culture water quality were determined in a commercial recirculating aquaculture system (RAS) for Japanese eel, Anguilla japonica. The particulate phase of the culture water was serially divided into six size fractions using 300, 200, 100, 75, 45, and 26 ${\mu}m$ pore size stainless sieves. The total, dissolved, and particulate nitrogen and phosphorus, and suspended solids for each fraction were determined. The concentration ranges in the fractions were: total nitrogen, 164-148 mg $L^{-1}$; total phosphorus, 20.4-15.5 mg $L^{-1}$; and total suspended solids, 8.1-6.1 mg $L^{-1}$. The concentration of total nitrogen and total phosphorus decreased significantly (P<0.05) with a 26 ${\mu}m$ and 200 ${\mu}m$ filter pore size, respectively. Nutrients from dissolved organic substances were much higher than from particulates. Analysis of particle size fractionation and its effects on water quality is useful to estimate removal efficiencies of a commercial effluent screening device for solid management and development of solid removal systems.