본 연구에서는 식이단백질 수준이 성장기 흰쥐의 신장 발달과 기능에 미치는 영향을 조사하기 위해 평균체중이 97.5$\pm$1.9g인 숫컷 흰쥐를 5%, 15% 그리고 50% casein 식이로 6주간 사육하였다. 체중증가량은 단백질 수준이 높을수록 높았으며 특히 단백질수준이 신장무게에 미친영향이 간, 비장, 흉선 등이 다른 장기에 비해 컸다. 신장내 총 DNA와 RNA 함량에서는 유의적인 차이가 없었다. 총 protein과 단위 g당 protein 함량은 식이내 단백질수준이 높을 수록 증가했다. 사구체여과율(GFR)은 식이단백질 수준이 높을수록 높았으나 신혈류(RBF)는 각 군 사이에 유의적인 차이를 보이지 않았다. 뇨질소배설량은 단백질 수준에 비례하여 유의적인 차이를 보였고 노칼슘배설량은 5%군이 낮은 경향을 보였다. 본 연구 결과로 성장기의 흰쥐에서 고단백식이는 신장의 무게증가를 촉진하며 사구체여과율을 증가시킴을 알 수 있다.
Min, T.S.;Kim, J.D.;Lee, J.H.;Hyun, Y.;Sohn, K.S.;Han, In K.
Asian-Australasian Journal of Animal Sciences
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제14권4호
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pp.525-534
/
2001
A total of 120 pigs were used to investigate the effects of yucca extracts on the growth performance, nutrient digestibility, nutrient excretion and carcass characteristics of finishing pigs fed different levels of dietary protein. Pigs were allotted into $2{\times}3$ factorial design by the supplementation of yucca extracts (YE, 0 and 120 mg/kg) and 3 levels of dietary protein (14, 16, 18% for early finisher and 12, 14, 16% for late finisher for low, medium and high protein diet, respectively). During the early finishing period (51~76 kg BW), no significant difference was found in growth performance regardless of the YE supplementation or dietary protein levels. Growth performance of late finishing pigs (76~101 kg BW) was also not significantly different among treatments. However, ADG of pigs fed YE diet was significantly improved (p<0.05) regardless of the dietary protein levels. For the overall period (51~101 kg BW), although adding YE to the diet and elevating the protein level showed better ADG, there were no significant differences on growth performance among treatments. Early finishers showed significantly higher crude protein, crude ash and crude fat digestibilities when they were fed diets supplemented with YE. Digestibilities of amino acids were not affected by YE. Late finishers did not show any significant differences in proximate nutrient digestibilities regardless of YE supplementation or dietary protein levels. YE tended to slightly improve the CP digestibility, however no significant difference was found with increased dietary protein levels. There was no significant difference in amino acid digestibilities with YE supplementation or dietary CP levels during the late finishing period. Dry matter (DM) and nitrogen (N) excretion in feces did not show any significant difference among treatments. Early finishing pigs also did not respond to the inclusion of YE or dietary protein levels (p<0.05). Fecal N excretion of early finishing pigs seemed to be lowered in pigs fed YE. Pigs fed medium dietary protein diet tended to excrete a higher amount of N during the early finishing period, but not statistically different. A slight increase in fecal N excretion was found with the increased level of dietary protein during the late finishing period. For ammonia nitrogen excretion, although there was no significance, the NH3-N content tended to be increased by the increased dietary protein levels and with YE supplementation. The NH3-N content in manure increased by 24.5% with YE supplementation. There were no significant differences in carcass weight, backfat thickness, carcass grade and loin eye area among treatments. However, pigs fed non-YE with low protein diet showed a significantly (p<0.05) low carcass ratio among treatments and there was significant (p<0.05) difference between the YE-added treatment and non YE treatment in carcass ratio. As for the feed cost, the cost of feeding high level protein was higher than that of medium level protein by 5% and low level protein by 9% (p<0.05). Therefore, based on this study, it could be concluded that environmentally friendly agents might play a role to some extent in finishing pigs from the aspect of pollution control, and that more than 14 and 12% of dietary protein for early finishing and late finishing pigs respectively do not necessarily guarantee high growth performance.
This study was conducted to determine the effects of different copper (inorganic and organic) and energy (tallow and glycerol) sources on growth performance, nutrient digestibility, gas emission, diarrhea incidence, and fecal copper concentration in growing pigs by using a 2${\times}$2 factorial design. In this trial, 96 pigs (63 d of age) were employed, with an average initial weight of 28.36${\pm}$1.14 kg. The dietary treatments were i) basal diet with 134 ppm copper (Korea recommendation) as $CuSO_4$+tallow; ii) basal diet with 134 ppm Cu as $CuSO_4$+glycerol; iii) basal diet with 134 ppm copper as CuMet+tallow; and iv) basal diet with 134 ppm copper as CuMet+ glycerol. Throughout the entire experimental period, no differences were noted among treatment groups with regard to the magnitude of improvement in ADG (average daily gain), ADFI (average daily feed intake) and G/F (gain:feed) ratios. The nitrogen (N) digestibility of pigs fed on diets containing organic copper was improved as compared with that observed in pigs fed on diets containing inorganic copper (p<0.05). An interaction of copper${\times}$energy was observed in the context of both nitrogen (p<0.05) and energy (p<0.01) digestibility. Ammonia emissions were significantly lower in the organic copper-added treatment groups than in the inorganic copperadded treatment groups (p<0.05). Mercaptan and hydrogen sulfide emissions were reduced via the addition of glycerol (p<0.05). No significant effects of copper or energy source, or their interaction, were observed in reference to diarrhea appearance and incidence throughout the entirety of the experimental period. The copper concentration in the feces was significantly lower in the organic copper source treatment group than was observed in the inorganic copper source treatment group (p<0.05). The results of this experiment show that organic copper substituted for inorganic copper in the diet results in a decreased fecal copper excretion, but exerts no effect on performance. The different energy (tallow and glycerol) sources interact with different copper sources and thus influence nutrient digestibility. Glycerol supplementation may reduce the concentrations of odorous sulfuric compounds with different Cu sources.
To investigate the effects of molasses addition at ensiling on nutritional quality of wilted napier grass, chemical quality and nutrient composition of the silages, digestibility and nitrogen retention at feeding trials were analysed using 4 goats in a cross over design. The results are as follows : 1. Molasses addition at ensiling decreased pH value (3.99) and ammonia nitrogen, and increased lactic acid content by 285% compared to non-additive silage (83.5 g/kg dry matter). 2. There were no differences in digestibilities of dry matter, crude protein, neutral detergent fiber, acid detergent fiber, hemicellulose and cellulose between the silage ensiled with molasses (MS silage) and the silage ensiled without molasses (WS silage). Urinary nitrogen excretion, however, significantly (p<0.05) decreased in goats fed the MS silage, and nitrogen retention was positive in goats fed the MS silages, but negative in goats fed the WS silage. 3. Acetic acid concentration in remained fluids in goats fed the MS silage was lower and propionic and butyric acid concentrations were higher than those in goats fed the WS silage. As water soluble carbohydrate content was higher in the MS silage than in the WS silage, a part of added molasses was still remained in the silage at the feeding trials and could be utilized for energy sources by the goats. Nitrogen may be also effectively utilized in goats fed the MS silage, because the silage were inhibited in proteolysis during ensiling.
This study was designed to find out the nutritional defect of general Korean diet and the effective way of nutritional supplementation. Seventy weanling Sprague-Dawley male rats weighing 51.8$\pm$0.9g were blocked into ten groups and fed ten different diets ad libitum for eight weeks: Standard groups(st gp) was given 72% sugar-20% casein diet: Cereal-vegetable group(c-v gp) was fed cereal-vegetable diet(c-v diet) composed of rice, barley, soybean, spinach and cabbage: the other eight groups were fed c-v diets supplemented with casein, vitamin $B_{2}$, calcium, vitamin A, vitamin B2 and A, vitamin A and calcium, vitamin $B_{2}$ and calcium, vitamin A and $B_{2}$and calcium, respectively, on the basis of each nutrients content of standard diet. The results were as follows: 1. Food intakes and body weight gains in all the experimental groups were significantly lower than st gp. Among experimental groups, casein gp and vit B2 gp tended to gain more body weights than c-v gp. 2. Through all the experimental period, F.E.R., pp.E.R., and NDPcal% did nod show significant differences among all the experimental groups and st gp. 3. The weights of liver, kidney, and gastrocnemius were significantly lower in all the experimental groups as compared with st gp. But brain and sex organ weights did not show differences among all the groups. 4. Nitrogen contents of total carcass, liver, and gastrocnemius in all the experimental groups tended to be increased as compared with st gp, and among experimental groups, they tended to be increased by casein supplementation and decreased by ca supplementation. 5. Apparent nitrogen digestibility, urinary and fecal nitrogen excretion, the amount of nitrogen retained, and N.P.U. did not show any significant differences among all the groups. 6. Serum total protein concentrations did not show any significant differences among all the groups.
The objective of this study was to evaluate the potential of an alumninosilicate clay, marketed under the trade name Biotite V, to improve growing-finishing pig performance and to determine its effects on nutrient digestibility and excretion. Sixty crossbred pigs (22.3${\pm}2.7kg$, Camborough 15 Line female${\times}$Canabred sire) were assigned on the basis of sex, weight and litter to one of four dietary treatments in a $2{\times}4$ (two sexes and four treatments) factorial design experiment. The experimental diets were based on barley and soybean meal and contained 0, 0.25, 0.5 or 0.75% biotite during the growing period (22.3-60.5 kg) and 0, 0.5, 1.0 or 1.5% biotite during the finishing period (60.5-110.3 kg). Each pig was allowed access to its own individual feeder for 30 min twice daily (07:00 and 15:00 h). Individual pig body weight, feed consumption and feed conversion were recorded weekly. The pigs were slaughtered at a commercial abattoir when they reached an average weight of 110.3 kg. Carcass weight was recorded and dressing percentage calculated. Carcass fat and lean measurements were obtained with a Destron PG 100 probe between the 3rd and 4th last ribs, 70 mm of the midline. Total tract digestibility coefficients for dry matter, energy, nitrogen and phosphorus were determined using three males and three females per treatment starting at an average weight of $52.2{\pm}3.8kg$. These pigs were housed under identical conditions as those used in the growing stage and were fed the same diets modified only by the addition of 0.5% chromic oxide as a digestibility marker. Over the entire experimental period (22.3-110.3 kg), daily gain was unaffected (p>0.05) by the inclusion of biotite in the diet. There was a cubic response for feed intake (p=0.06) and a quadratic response (p=0.07) for feed conversion due to biotite. Feeding biotite produced no significant (p>0.05) linear or quadratic effects on any of the carcass traits measured. Dry matter digestibility decreased linearly (p=0.02) with increasing levels of biotite in the diet. However, digestibility coefficients for energy, nitrogen and phosphorus were unaffected (p<0.05) by biotite inclusion. Lactobacilli and enterobacteria numbers were unaffected by inclusion of biotite while Salmonella was not detected in any of the fecal samples. The overall results of this experiment indicate that biotite inclusion did not reduce fecal excretion of nitrogen or phosphorus and failed to improve nutrient digestibility. Neither growth rate nor carcass quality was improved while a modest improvement in feed conversion was observed at lower levels of inclusion. Based on the results of this experiment, it would be difficult to justify the routine inclusion of biotite in diets fed to grower-finisher pigs. Whether or not a greater response would have been obtained with pigs of a lower health status is unknown.
The small and the large intestine of swine represent the organs that extract nutrients from feedstuffs through digestion and fermentation and that allow their absorption and incorporation into the blood circulation. Special attention is directed towards the small intestine of young pigs since the transition to a solid diet at weaning exerts major impacts on the structural and functional integrity of the small intestine. Dietary factors involved in postweaning changes of gut morphology and biochemistry such as removal of bioactive compounds in sows milk at weaning, anti-nutritional factors in weaner diets, dietary fiber and the role of voluntary feed intake will be elucidated. The microbial function of the large intestine which is carried out by a diverse population of microorganisms is dependent on substrate availability. Short chain fatty acids as main fermentation products contribute to the energy supply of the host but they are also important for the maintenance of the morphological and functional integrity of the epithelium in the colon. As a result of bacterial nitrogen assimilation in the large intestine, nitrogen is shifted from the urinary to the fecal excretion route thus saving metabolic energy to the pig because less ammonia would become available for conversion to urea.
Three goats were fed with $^{15}N$-labelled rice straw to study the characteristics of digestion, assimilation, metabolism and excretion of C and N compounds from rice straw. It was shown that the amount of $^{15}N$ assimilated into the bodies of the two slaughtered goats accounted for 38.5 and 23.6% of the total amount of $^{15}N$ ingestion in the experimental diet by each goat. The $^{15}N$ excreted via the feces and urine for the three goats accounted for 34.8, 33.2 and 33.9% of the total amount of $^{15}N$ ingested in the feed of the 3 goats. The recoveries of $^{15}N$ from the two slaughtered goats were 73.3 and 57.5%, with the corresponding rates of $^{15}N$ loss 26.7 and 42.5% respectively. The amino acids digestibilities were 68.7 and 54.0%, while the digestibilities of carbohydrates were 74.8 and 67.7% respectively for goats 1 and 3.
Park, Myung-G.;Yang, Sung-R.;Shim, Jae-H.;Hong, Gi-H.;Chung, Chang-S.;Yang, Dong-B.;Cho, Byung-C.
Journal of the korean society of oceanography
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제39권1호
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pp.20-25
/
2004
The Yellow Sea is known to be a very productive region in terms of fisheries. However, its trophic status seems to be highly variable, ranging from oligotrophic to eutrophic, based on new production (NP) values. The NP and regenerated production (RP) values estimated from $^{15}N$-labelled nitrate and ammonium uptake in spring (April 1996) and winter (February 1997) during this study ranged from 0.05 to 19.8 mg $N m^{-2} d^{-1}$ and from 0.1 to 22.8 mg $N m^{-2}d^{-1}$, respectively. Our measurements and earlier observations suggested that NP in the Yellow Sea varied over the four orders of magnitude (range 0.05-180.9 mg $N m^{-2} d^{-1}$) temporally and spatially, and that RP (range 0.1-507.5 mg $N m^{-2}d^{-l}$) based on ammonium predominated during most period of the year, except in winter when both productions were low. The significant nitrogen uptake by phytoplankton below the euphotic zone and episodic entrainment of phytoplankton from below the euphotic zone into the euphotic zone, and nitrite excretion and dissolved organic nitrogen release during nitrate uptake might explain the apparent dominance of RP in the Yellow Sea.
Nine crossbred castrated lambs fitted with rumen and duodenum cannula and fed a diet of hay and concentrate formulated with ground corn and soybean meal as main ingredients were used to assess the duodenal ideal amino acid pattern. Three synthetic amino acid mixtures with different profile of essential amino acids were duodenally infused in order to get three different amino acid patterns flowing into the duodenum. The mixtures were designed to have similar amino acid profile as rumen microbial protein (Pm), casein (Pc) and modified muscle amino acid (Pmm). Results showed a lower urine nitrogen excretion (p=0.05), a higher nitrogen retention (p=0.04) and bodyweight gain with treatment Pmm. The modified muscle amino acid pattern also promoted a lower ratio of Gly to other amino acids in plasma (Gly/OAA) and a higher RNA and RNA/DNA concentration in the liver of the sheep. Meanwhile, the urea concentration in plasma was reduced and the insulin concentration was increased with Pmm treatment. No differences in glucose and growth hormone concentration in plasma were found among three treatments. All results obtained indicate that the modified muscle amino acid pattern (Lys 100%, Met+Cys 39%, Thr 76%, His 41%, Arg 72%, Leu 158%, Ile 81%, Val 105%, Phe 81% and Trp 13%) was the best for growing sheep.
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