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http://dx.doi.org/10.5713/ajas.2002.38

A Study on the Optimal Amino Acid Pattern at the Proximal Duodenum in Growing Sheep  

Wang, Hongrong (Institute of Animal Nutrition and Feeds Science, Inner Mongolian Academy of Animal Science)
Lu, Dexun (Institute of Animal Nutrition and Feeds Science, Inner Mongolian Academy of Animal Science)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.15, no.1, 2002 , pp. 38-44 More about this Journal
Abstract
Nine crossbred castrated lambs fitted with rumen and duodenum cannula and fed a diet of hay and concentrate formulated with ground corn and soybean meal as main ingredients were used to assess the duodenal ideal amino acid pattern. Three synthetic amino acid mixtures with different profile of essential amino acids were duodenally infused in order to get three different amino acid patterns flowing into the duodenum. The mixtures were designed to have similar amino acid profile as rumen microbial protein (Pm), casein (Pc) and modified muscle amino acid (Pmm). Results showed a lower urine nitrogen excretion (p=0.05), a higher nitrogen retention (p=0.04) and bodyweight gain with treatment Pmm. The modified muscle amino acid pattern also promoted a lower ratio of Gly to other amino acids in plasma (Gly/OAA) and a higher RNA and RNA/DNA concentration in the liver of the sheep. Meanwhile, the urea concentration in plasma was reduced and the insulin concentration was increased with Pmm treatment. No differences in glucose and growth hormone concentration in plasma were found among three treatments. All results obtained indicate that the modified muscle amino acid pattern (Lys 100%, Met+Cys 39%, Thr 76%, His 41%, Arg 72%, Leu 158%, Ile 81%, Val 105%, Phe 81% and Trp 13%) was the best for growing sheep.
Keywords
Amino Acids; Duodenum; Growing Sheep;
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1 Cole, D. J. A. 1979. Amino acids nutrition of the pig. In: Recent Advances in Animal Nutrition-1978. [W. Haresign and Lewis, editors], London: Butterworths pp. 59-72.
2 Fuller, M. F., R. M. Livingstone, B. A. Baird and T. Atkinson. 1979. The optimal amino acid supplementation of barley for the growing pig. 1. Response of nitrogen metabolism to progressive supplementation. Br. J. Nutr. 41:321-331.   DOI   PUBMED   ScienceOn
3 Harper, A. E. 1964. Amino acid toxicities and imbalance. In:Mammalian Protein Metabolism (Ed. H. N. Muaro and J. B. Allison). Vol. 1. Academic Press. New York.
4 Monro, H. N. and A. Flack. 1969. Analysis of nitrogenous constituents. Measurement of nucleic acids in tissues. In:Mammalian Protein Metabolism. Academic Press, NY. Vol. 3, p. 477.
5 Ni, Zhanming and Qiu Bili. 1990. A procedure of assessing plasma urea nitrogen in biological samples. In: Clinic Biochemical Test. Shanghai Science and Technology Press, Shanghai.
6 Pfander, W. H., S. E. Grebing, C. M. Price, O. Lewis, J. M. Asplund and C. V. Ross. 1975. Use of plasma urea nitrogen to vary protein allowances of lambs. J. Anim. Sci. 41:647.   DOI   PUBMED
7 Storm, E. 1982. Isolation and nutritive value of rumen microorganisms and their limiting amino acids for growing sheep. Ph.D. Thesis. University of Aberdeen. p. 486.
8 Storm, E. and E. R. Orskov. 1984. The nutritive value of rumen microorganisms in ruminants. 4. The limiting amino acids of microbial protein in growing sheep determining by a new approach. Br. J. Nutr. 52:613-620.   DOI   PUBMED   ScienceOn
9 William, H. H., L. Curtin, J. Abraham, J. K. Loosli and L. A. Maynard. 1954. Estimation of growth requirements for amino acids by assay of the carcass. J. Biol. Chem. 208:277.
10 Wang, H. R, D. X. Lu, H. Y. Zhang and X. Y. Zhao. 1998. Determination of amino acid digestibility in the small intestine of growing sheep. Inner Mongolian Journal of Animal Science and Production. 19(2):18-21.
11 Wang, H. R. and D. X. Lu. 1999. A Study on Limiting Amino Acids for Growing Sheep Fed a Corn Based Diet. Journal of Animal Nutrition (ACTA ZOONUTIMENTA SINICA). 11(4):17-28.
12 Hogan, J. P., R. H. Weston and J. R. Lindsay. 1968. Influence of protein digestion on plasma amino acids level in sheep. Australian J. Biol. Sci. 21:1263.   DOI   PUBMED
13 Trenkle, A. and D. G. Topel. 1978. Relationships of some endocrine measurements to growth and carcass composition of cattle. J. Anim. Sci. 46:1604-1609.   DOI
14 Egan, A. R. 1972. Plasma and urinary metabolites as indices of nitrogen utilization in sheep. Proc. New Zealand Soc. Anim. Prod. 32:87.
15 Fenderson, C. L. and W. G. Bergen. 1975. An assessment of essential amino acid requirements of growing steers. J. Anim. Sci. 41:1759.   DOI   PUBMED
16 NRC. 1988. Nutrient requirements of swine. 9th. Rev. Ed Washington DC. National Academy Press.
17 Fraser, D. L., E. R. Orskov, F. G. Whitelow and M. F. Franklin. 1991. Limiting amino acids in dairy cows given casein as the sole source of protein. Livest. Prod. Sci. 28:235-252.   DOI   ScienceOn
18 Leng, R. A. 1982. Modification of rumen fermentation. In:Nutritional Limits to Animal Production from Pastures (Ed. J. B. Hacker). Commonw. Agric. Bur., Farnham Royal, U.K.
19 ARC. 1981. The nutrient requirement of pig. Slough: Commonwealth Agricultural Bureau.
20 Klusmeyer, T. H., Jr. R. D. Macarthy, J. H. Clark and D. R. Nelson. 1990. Effect of source and amount of protein on ruminant fermentation and passage of nutrients to the small intestine of lactating cows. J. Dairy Sci. 73:3562.
21 Reis, P. J., D. A. Tunks and L. F. Sharry. 1973. Plasma amino acid pattern in sheep receiving abomsal infusion of methionine and cystine. Australian J. Biol. Sci. 26:635.   DOI   PUBMED
22 McCance, R. A. and E. Widdowson. 1978. The Composition of Foods. 4th ed (Ed. D. A. T. Southgate and A. A. Paul). HMSO, London.
23 Reeds, P J., S. M. Hay, P. M. Dorwood and R. M. Palmer. 1986. Simulation of muscle growth by clenbuterol: lack of an effect on muscle protein biosynthesis. Br. J. Nutr. 56:248.   DOI   ScienceOn
24 SAS, 1987. SAS User's Guide, Statistics, Version 6 Edition, SAS Ins. Inst., Cart, NC.
25 Ludden, N. A. and M. J. Cecava. 1995. Supplemental protein sources for steers fed corn-based diets: 1. Rumial characteristics and intestinal amino acid flows. J. Anim. Sci. 73:1324-1333.
26 Youny, A. W., G. T. Schelling, E. R. Tucker and Jr. G. E. Mitchel. 1981. Plasma amino acids response to abomasal infusion of amino acids in sheep. J. Anim. Sci. 52:1421-1429.   DOI
27 Beermann, D. H., W. R. Butler, D. E. Hogue, V. K. Fishell, R. H. Dalrymple, C. A. Ricks and C. G. Scanes. 1987. Cimaterolinduced muscle hypertrophy and altered endocrine status in lambs. J. Anim. Sci. 65:1514.   DOI   PUBMED
28 Cecava, M. J., N. R. Merchen, L. L. Berger and Jr. Fahey. 1990. Intestinal supply of amino acids in sheep fed alkaline hydrogen peroxide-treated wheat straw-based diets supplemented with soybean meal of combination of corn gluten meal and blood meal. J. Anim. Sci. 88:467.   DOI   ScienceOn
29 Eversole, D. E., W. G. Bergen, R. A. Merkel, W. T. Magee and H. W. Harpster. 1981. Growth and muscle development of feedlot cattle of different genetic backgrounds. J. Anim. Sci. 53:91-101.   DOI   PUBMED
30 Gao Ming and Feng Zhongzi. 1993. A modified method for the analysis of chromium in feeds and faces samples. Inner Mongolian Anim. Sci. Prod. 3:42-43.
31 Reeds, P. J. and T. A. Davies. 1992. Hormonal regulation of muscle protein synthesis and degradation. In: The Control of Fat and Lean Deposition (Ed. P. J. Buttery, K. N. Boorman and D. B. Lindsay). pp. 1-26.
32 LaBarca, C. and K. Paigen. 1978. A simple, rapid and sensitive DNA assay procedure. Anal. Biochem. 102:344.   DOI   ScienceOn
33 Meister, A. 1965. Biochemistry of Amino Acids. Vol. 1, Academic Press, New York.
34 AOAC, 1984. Official Methods of Analysis (13th Ed). Association of Official Analytical chemists, Washington DC.
35 D'Mello, J. P. F. 1994. Amino acid imbalances, antagonisms and toxicities. In: Amino Acid in Farm Animal Nutrition (Ed. J. P. F. D'Mello).