• Title/Summary/Keyword: Nitrogen (N) limitation

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Effects of Nitrogen Fertilization Levels and Some Additives on the Chemical Composition and Silage Quality of Forage Rape (Brassica napus Subsp. oleifera) I. Effects of harvestinh dates and N forage rape (질소시비수준과 첨가제가 사초용 유채의 사료가치 및 사일리지의 품질에 미치는 영향 I. 예취시기 및 질소시비수준이 수량 및 사료가치에 미치는 영향)

  • 조무환;김동암
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.8 no.1
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    • pp.33-39
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    • 1988
  • Insufficient herbage during the fall in the temperate areas of the world has been a serious limitation in animal production, but leafy brassicae are potentially very useful for extending the grazing season when the growth and quality of grass is poor. This study was conducted to determine the effects of cutting stage and N fertilization on dry matter (DM) yield, forage quality and chemical composition of forage rape (Brassica napus Subsp. oleifera). The experiment was conducted in the Experimental Livestock Farm of Seoul Nat'l Univ., at Suweon, during 1985- 1986. The results obtained are summarized as follows: 1. DM percentage and yield significantly increased as the growth period was prolonged and decreased as the N fertilization increased, but in-vitro dry matter digestibility (IVDMD) was unaffected by the treatments. 2. Plant height, DM yield and $NO_3$-N content significantly increased as the rate of N fertilization increased and DM percentage decreased, but the IVDMD was unaffected. 3. The forage rape had high percentage of crude protein and ash. Glutamic acid, pro!ine and aspartic acid were the major amino acids. Concentration of P, Ca and Mg were high.

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Microcystin Production by Microcystis sp. under N or P Limitation

  • Oh Hee-Mock;Kim Jee-Hwan
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2001.11a
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    • pp.113-120
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    • 2001
  • The production of microcystins from Microcystis aeruginosa was investigated in a P-limited continuous culture and a batch culture. The microcystin content of M aeruginosa was higher at a lower $\mu$, whereas the microcystin (MC)-producing rate was linearly proportional to $\mu$. The ratios of the MC-producing rate to the C-fixation rate were higher at a lower $\mu$. Consequently, increases in the microcystin content per dry weight along with the production of the more toxic form, MC-LR, were both observed under more P-limited conditions. The microcystin content of M. aeruginosa exhibited a high correlation with the total N content regardless of N-fixed or P-fixed culture. The microcystin concentration was investigated from spring to autumn in 1999 in the Daechung Reservoir, Korea. The dominant species in the algal blooming season was Microcystis. When the microcystin concentration exceeded about 100 ng $1^{-1}$ the ratio of particulate to dissolved total nitrogen (TN) or total phosphorus (TP) interestingly converged at a value of 0.6. The microcystin concentration was lower than 50 ng $1^{-1}$ at a particulate N:P ratio below 8, whereas the microcystin concentration varied quite substantially from 50 ng $1^{-1}$ to 250 ng $1^{-1}$ at a particulate N:P ratio> 8.

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Nitrogen Removal in Fluidized Bed and Hybrid Reactor using Porous Media (다공성 담체를 이용한 유동상 및 하이브리드 반응기에서의 질소제거)

  • Jun, Byong-Hee
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.5
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    • pp.542-548
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    • 2005
  • A fluidized bed reactor containing porous media has been known to be effective for nitrogen and organic matters removal in wastewater. The porous media which attached microbes plays important roles in simultaneous nitrification/denitrification (SND) due to coexistence of oxic, anaerobic and anoxic zone. For SND reaction, oxygen and organic substrates should be effectively diffused from wastewater into the intra-carrier zone. However, the overgrowth heterotrophic microbes at the surface of porous media may restrict from substrates diffusion. From these viewpoints, the existence and effect of heterotrophic bacteria at surface of porous media might be the key point for nitrogen removal. A porous media-membrane hybrid process was found to have improved nitrogen removal efficiency, due to stimulated denitrification as well as nitrification. Microelectrode studies revealed that although intra-media denitrification rate in a conventional fluidized bed was limited by organic carbon, this limitation was reduced in the hybrid process, resulting in the increased denitrification rate from 0.5 to $4.2\; mgNO_3-N/L/hr$.

Improvement of Single Anaerobic Reactor for Effective Nitrogen Removal (효율적 질소제거를 위한 단일 혐기성반응조의 개선)

  • 한동준;류재근;임연택;임재명
    • Journal of environmental and Sanitary engineering
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    • v.12 no.3
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    • pp.9-17
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    • 1997
  • This research aims to remove nitrogen in the piggery wastewater by combined process with upflow anaerobic sludge blanket (UASB) and biofilm process. For the effective denitrification. anaerobic and anoxic reactors were connected to a reactor. The effluent of aerobix reactor was recycled equally with influent in the upper filter of anaerobic reactor for denitrification and outlet of UBF reactor was connected to the settling tank with $1.5{\;}{\ell}$ capacity and the settling sludge was repeatedly recycled to UASB zone. The organic loading rate of total reactor was operated from 0.4 to $3.1kgCOD/m^{3}/d$ and it was observed that the removal rate of TCOD was 80 to 95 percentage. Ammonia nitrogen was removed over 90 percentage in the less volumetric loading rate than $0.1{\;}kgN/m^{3}/d$. But because of non-limitation of organic materials, it was reduced to 70 percentage in the more volumetric loading rate than $0.6{\;}kgN/m^{3}/d$. But denitrification rate was observed 100 percentage in the all of loading rate. This is caused by the maintenance of optimum temperature, sufficient carbon source, and competition of electron acceptors. The results of COD mass balance at the $1.21{\;}kgCOD/m^{3}/d$ was observed with the 71.7% percentage of influent COD. It was revealed that the most part of organic materials was removed in the aerobic and the anaerobic reactor because 38.4 percentage was conversed into $CH_{4}$ gas and 11 percentage was removed in the aerobic reactor with cell synthesis and metabolism. Besides, 5.7% organics was used to denitrification reaction and 3.7% organics related to sulfate reduction.

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Screening of Bioactive Materials from Freshwater Microalgae (담수산 미세조류로부터 생리활성물질의 탐색)

  • Lee, Wan-Seok;Choi, Ae-Ran;Ahn, Chi-Yong;Oh, Hyun-Cheol;Ahn, Jong-Seog;Oh, Hee-Mock
    • ALGAE
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    • v.19 no.3
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    • pp.271-276
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    • 2004
  • One hundred and fifty four micro algal strains, newly isolated from nationwide freshwaters in Korea, were screened for their anticancer, ant diabetic, and antibiotic activities. The micro algal strains were cultured with different nutritional conditions that were divided into 4 groups as follows; a normal Allen medium, nitrogen (N)-limited medium, phosphorus (P)-limited medium, and N and P-limited medium. Algal biomass was extracted with a mixture of acetone:H₂O (1:1, v:v) and the extracts were used for the screening of bioactive materials. Anticancer, ant diabetic, and antibiotic materials were screened by the methods of vaccinia Hl-related protein tyrosine phosphates (VHR DS-PTPase) inhibition, protein tyrosine phosphates 1B (PTP1B) inhibition, and paper disk. The inhibition activity of VHR DS-PTPase was observed in 18 strains, having a maximum 79% inhibition from Anabaena affinis and the inhibition activity of PTP1B was observed in 9 strains, having a maximum 97% from Sphaerocystis schroeteri. Microcystis aeruginosa incubated in an N and P-limited medium showed antibiotic activity in 8 species out of 13 pathogenic bacteria. As a whole, it seemed that the stressed condition such as N and/or P limitation increased the production of bioactive materials in micro algae.

Comparison of Water Quality According to Seasonal Variation in Mokpo and Wando Costal Areas

  • Kim, Woo-Hang;Lee, Young-Sik
    • Journal of Environmental Science International
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    • v.17 no.3
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    • pp.269-273
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    • 2008
  • The objective of this study was to evaluate the relationship between nutrients and phytoplankton. This study was done by the comparison to two costal areas Mokpo, which inflow fresh water, and Wando. In August, salinity of the sea water decreased by 3.5-4.5%o in Mokpo coastal area, but was not nearly decreased in Wando coastal area. This suggests a lot of fresh water inflow in Mokpo coastal area. DIN and DIP were decreased by water temperature increasing in Wando. However, in Mokpo, DIN and DIP were increased greatly during the summer season. Nitrogen was limited to a 10 NIP ratio especially during the summer season in Wando coastal area while phosphorus in Mokpo coastal area was limited with over 28 N/P ratio in all the seasons. Coefficient of determination$(r^2)$ between DIP and Chl.-a was 0.91 in Mokpo coastal area. On the other hand, Coefficient of determination$(r^2)$ between Chl.-a and DIN, DIP were 0.93 and 0.89, respectively, in Wando coastal area. These results suggest DIP in Mokpo and DIN and DIP in Wando might be limited at the increase of phytoplankton.

Studies on the Mixture combination in rermanent Pasture I. Effects of nitrogen fertilization and cutting management on the botanical composotion (영년채초지에 있어서 혼파조합에 관한 연구 I. 예취빈도와 질소시비수준이 식생구성에 미치는 영향)

  • ;H. Jacob
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.9 no.2
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    • pp.68-76
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    • 1989
  • The effect of characteristic of mixture sowing combination on the development of botanical composition was conducted for 12 years on the meadow experiment. The results of this study were summarized as follows. 1. Festuca pratensis lost the expected position of a dominant species. Its vegetation ratio decreased gradually until the last survey year. 2. Arrhenatherum elatius, known as a typical 2-cutting grasss, developed to a dominant species only in the 3- cutting plot without limitation. 3. Alopecurus pratensis, whose feeding value diminishes rapidly, developed to a dominant species regardless of N-fertilization under the condition of 3-cutting, even though the same small amount of seeding was introduced as usual. 4. Trisetum flavescens which causes calcinose exceeded the 40% of vegetation ratio which was the approved rate for feeding at the high N-level (2OOKgIha). 5. Among the introduced, the species showed the significance in quantity were Lotus corniculafus solely in the 2-cutting plot, and Trifolium repens solely in the 3-cutting plot. In the 3-cutting plot legume yet remained under N-fertilization. 6. The appropriate ratio of vegetation for the subordinate species in the mixture sowing combination was easily attained from the least amount of seeding (Gramineae with max. 3Kg/ha, Leguminosae with max. 2kgIha). 7. Into the mixture sowing combination, infiltrated unintroduced species marked more noticeably in the 2- cutting plot than in the 3-cutting plot.

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Evaluation of Water Quality Variation and Sediment of a Shallow Artificial Lake (Lake llgam) in Located the Metropolitan Area (도심의 얕은 인공호인 일감호의 수질변화특성과 퇴적환경의 평가)

  • Kim, Ho-Sub;Ko, Jae-Man;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.36 no.2 s.103
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    • pp.161-171
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    • 2003
  • The present study evaluated water quality variation, limiting nutrient, and sediment of a shallow eutrophic lake (Lake Ilgam) in the metropolitan area from 2000 to 2002. According to annual mean chl.a ($77.2{\pm}36.6\;{\mu}g/l) and TP ($66.6{\pm}20.5\;{\mu}g/l) concentration and trophic state index (>60), Lake llgam was in very eutrophic status. Both inorganic nitrogen ($NH_3-N$ and $NH_3-N$) and phosphorus (SRP) concentrations in the water column increased during winter and spring, but decreased during summer followed by the phytoplankton development. Evidence for phosphorus and nitrogen as being the potential limiting nutrients for phytoplankton growth was supported by the ratio of DIN/DIP (by mass) (${\sim}$835.8), TSI derivations analysis, and algal growth potential bioassay. Based on the results of TSI derivations, strong nutrient limitation by both N and P occurred from September to November when P content in sediment (114.6 mg P/kg) was relatively low compared with the summer. Sediment contained a large amount of nitrogen (TKN: 4,452${\pm}$283.0mg N/kg dry sediment). Phosphorus content in sediment (TP: 313${\pm}$155 mg P/kg) was relatively low with temporal change. P release rate (0.29${\pm}$0.02 mg $m^{-2}$ $day^{-1}$) was high under the aerobic condition at pH 9. These results indicate that the sediment could play an important role as a source of a limiting nutrient, and temporal change of P content in the sediment is closely related with water quality, especially algal biomass change in Lake llgam.

Limitation of Nitrogen ion Implantation and Ionplating Techniques Applied for Improvement of Wear Resistance of Metallic Implant Materials (금속 임플란트 소재의 내마모성 향상을 위하여 적용되는 질소 이온주입 및 이온도금법의 한계)

  • 김철생
    • Journal of Biomedical Engineering Research
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    • v.25 no.2
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    • pp.157-163
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    • 2004
  • Nitrogen ion implantation and ion plating techniques were applied for improvement of the wear resistance of metallic implant materials. In this work, the wear dissolution behaviour of a nitrogen ion implanted super stainless steel (S.S.S, 22Cr-20Ni-6Mo-0.25N) was compared with those of S.S.S, 316L SS and TiN coated 316L SS. The amounts of Cr and Ni ions worn-out from the specimens were Investigated using an electrothermal atomic absorption spectrometry. Furthermore, the Ti(Grade 2) disks were coated with TiN, ZrN and TiCN by use of low temperature arc vapor deposition and the wear resistance of the coating layers was compared with that of titanium. The chemical compositions of the nitrogen ion implanted and nitride coated layers were examined with a scanting auger electron spectroscopy. It wat observed that the metal ions released from the nitrogen ion implanted S.S.S surface were significantly reduced. From the results obtained, it was shown that the nitrogen ion implanted zone obtained with 100 KeV ion energy was easily removed within 200,000 revolutions from a wear dissolution testing under a similar load condition when applied to artificial hip joint. The remarkable improvement in wear resistance weir confirmed by the nitrides coated Ti materials and the wear properties differ greatly according to the chemical composition of the coating layers. for specimens with the same coating thickness of about 3$\mu\textrm{m}$, TiCN coated Ti showed the highest wear resistance. However, after removing the coating layers, the wear rates of all nitrides coated Ti reverted to their normal rates of below 10,000 revolutions from Ti-disk-on-disk wear testing under the same load condition. From the results obtained, it is suggested that the insufficient depth of the 100 Kel N$\^$+/ ion implanted zone and of the nitrides coated layers of 3$\mu\textrm{m}$ are subject to restriction when used as frictional parts of load bearing implants.

Effect of Nitrogen, Titanium, and Yttrium Doping on High-K Materials as Charge Storage Layer

  • Cui, Ziyang;Xin, Dongxu;Park, Jinsu;Kim, Jaemin;Agrawal, Khushabu;Cho, Eun-Chel;Yi, Junsin
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.33 no.6
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    • pp.445-449
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    • 2020
  • Non-volatile memory is approaching its fundamental limits with the Si3N4 storage layer, necessitating the use of alternative materials to achieve a higher programming/erasing speed, larger storage window, and better data retention at lower operating voltage. This limitation has restricted the development of the charge-trap memory, but can be addressed by using high-k dielectrics. The paper reviews the doping of nitrogen, titanium, and yttrium on high-k dielectrics as a storage layer by comparing MONOS devices with different storage layers. The results show that nitrogen doping increases the storage window of the Gd2O3 storage layer and improves its charge retention. Titanium doping can increase the charge capture rate of HfO2 storage layer. Yttrium doping increases the storage window of the BaTiO3 storage layer and improves its fatigue characteristics. Parameters such as the dielectric constant, leakage current, and speed of the memory device can be controlled by maintaining a suitable amount of external impurities in the device.