• Microbiology and Biotechnology Letters
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• v.48 no.4
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• pp.547-555
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• 2020

Microalgae are a promising resource in energy and food production as they are cost-effective for biomass production and accumulate valuable biological resources. In this study, CO2 assimilation, biomass, and lipid production of 4 microalgal species (Chlorella vulgaris, Mychonastes homosphaera, Coelastrella sp., and Coelastrella vacuolata) were characterized at different CO2 concentrations ranging from 1% to 9%. Microscopic observation indicated that C. vulgaris was the smallest, followed by M. homosphaera, C. vacuolata, and Coelastrella sp. in order of size. C. vulgaris grew and consumed CO2 more rapidly than any other species. C. vulgaris exhibited a linear increase in CO2 assimilation (up to 9.62 mmol·day-1·l-1) as initial biomass increased, while the others did not (up to about 3 mmol·day-1·l-1). C. vulgaris, Coelastrella sp., and C. vacuolata showed a linear increase in the specific CO2 assimilation rate with CO2 concentration, whereas M. homosphaera did not. Moreover, C. vulgaris had a greater CO2 assimilation rate compared to those of the other species (14.6 vs. ≤ 11.9 mmol·day-1·l-1). Nile-red lipid analysis showed that lipid production per volume increased linearly with CO2 concentration in all species. However, C. vulgaris increased lipid production to 18 mg·l-1, compared to the 12 mg·l-1 produced by the other species. Thus, C. vulgaris exhibited higher biomass and lipid production rates with greater CO2 assimilation capacity than any other species.

• Food Science of Animal Resources
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• v.33 no.3
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• pp.411-416
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• 2013

This study aimed to examine the mechanisms underlying the effects of Garcinia cambogia extract on the adipogenic differentiation of 3T3-L1 cells and long-chain saturated fatty acid-induced lipotoxicity of HepG2 cells. 3T3-L1 preadipocytes, mouse embryonic fibroblast-adipose like cell line, were treated with MDI solution (0.5 mM IBMX, 1 ${\mu}M$ dexamethasone, 10 ${\mu}g/mL$ insulin) to generate a cellular model of adipocyte differentiation. Using this cellular model, the anti-obesity effect of Garcinia cambogia extract was evaluated. MDI-induced lipid accumulation and expression of adipogenesis-related genes were detected by Oil red O staining, Nile Red staining, and Western blot analysis. Effects Garcinia cambogia extract on palmitate-induced lipotoxicity was also analyzed by MTT assay, LDH release, and DAPI staining in HepG2 cells. Garcinia cambogia extract significantly suppressed the adipogenic differentiation of preadipocytes and intracellular lipid accumulation in the differentiating adipocytes. Garcinia cambogia extract also markedly inhibited the expression of peroxisome proliferator- activated receptor ${\gamma}2$ ($PPAR{\gamma}2$), CCAT/enhancer-binding protein ${\alpha}$ ($C/EBP{\alpha}$), and adipocyte protein aP2 (aP2). In addition, Garcinia cambogia extract significantly attenuated palmitate-induced lipotoxicity in HepG2 cells. Palmitateinduced cellular damage and reactive aldehydes were also significantly reduced in the presence of Garcinia cambogia extract. These findings suggest that the Garcinia cambogia extract inhibits the adipogenic differentiation of 3T3-L1 preadipocytes, probably by regulating the expression of multiple genes associated with adipogenesis such as $PPAR{\gamma}2$, $C/EBP{\alpha}$, aP2, and thereby modulating fatty acid-induced lipotoxicity to reduce cellular injury in hepatocytes.

• Journal of Food Hygiene and Safety
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• v.35 no.1
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• pp.93-101
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• 2020

Seakso 1, a maize hybrid, was developed in 2008 by Gangwon Agricultural Research and Extension Services in Korea and registered in 2011. It is single-cross hybrid, semi-flint, deep-purple variety of corn, variety of are yellow, while the husks and cobs are purple. Due to the sensitivity of Seakso 1 to excess moisture after seeding, water supply should be carefully managed, and it should be harvested at a suitable time to obtain the highest anthocyanin content. This study investigated the hepatoprotective effect of Saekso 1 corn husk and cob extracts (EHCS) in oleic acid-induced non-alcoholic fatty liver disease (NAFLD) in HepG2 cells. EHCS showed a high level of lipid accumulation inhibiting effect. EHCS also suppressed triglyceride accumulation and inhibited expression of lipid marker genes, such as sterol regulatory element binding protein-1c (SREBP-1c) and sterol regulatory element binding protein-1a (SREBP-1a). Analysis by western blot of the expression of p-AMPK, p-SREBP1, PPARα, and FAS proteins showed that the incidence of SREBP1 protein, a major factor involved in lipid metabolism in the liver, has decreased significantly after treatment with the extracts. Moreover, the protein-induced expression of FAS, a major enzyme involved in the biosynthetic pathways of fatty acids, was decreased significantly in all concentrations. These results suggest that EHCS is a potent agent for the treatment of NAFLD.