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http://dx.doi.org/10.13103/JFHS.2020.35.1.93

Inhibitory Effect of Purple Corn 'Seakso 1' Husk and Cob Extracts on Lipid Accumulation in Oleic Acid- Induced Non-Alcoholic Fatty Liver Disease HepG2 Model  

Lee, Ki Yeon (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
Kim, Tae hee (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
Kim, Jai Eun (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
Bae, Son wha (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
Park, A-Reum (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
Lee, Hyo Young (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
Choi, Sun jin (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
Park, Jong yeol (Hongcheon Maize Experiment, Gangwon-do Agricultural Research and Experiment Services)
Kwon, Soon bae (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
Kim, Hee Yeon (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
Publication Information
Journal of Food Hygiene and Safety / v.35, no.1, 2020 , pp. 93-101 More about this Journal
Abstract
Seakso 1, a maize hybrid, was developed in 2008 by Gangwon Agricultural Research and Extension Services in Korea and registered in 2011. It is single-cross hybrid, semi-flint, deep-purple variety of corn, variety of are yellow, while the husks and cobs are purple. Due to the sensitivity of Seakso 1 to excess moisture after seeding, water supply should be carefully managed, and it should be harvested at a suitable time to obtain the highest anthocyanin content. This study investigated the hepatoprotective effect of Saekso 1 corn husk and cob extracts (EHCS) in oleic acid-induced non-alcoholic fatty liver disease (NAFLD) in HepG2 cells. EHCS showed a high level of lipid accumulation inhibiting effect. EHCS also suppressed triglyceride accumulation and inhibited expression of lipid marker genes, such as sterol regulatory element binding protein-1c (SREBP-1c) and sterol regulatory element binding protein-1a (SREBP-1a). Analysis by western blot of the expression of p-AMPK, p-SREBP1, PPARα, and FAS proteins showed that the incidence of SREBP1 protein, a major factor involved in lipid metabolism in the liver, has decreased significantly after treatment with the extracts. Moreover, the protein-induced expression of FAS, a major enzyme involved in the biosynthetic pathways of fatty acids, was decreased significantly in all concentrations. These results suggest that EHCS is a potent agent for the treatment of NAFLD.
Keywords
NAFLD; Purple corn; Husk; Cob; Oleic acid; HepG2;
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