• Journal of the Korean Society of Tobacco Science
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• v.11 no.2
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• pp.135-141
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• 1989

The experiment was conducted to obtain basic information on breeding of flue-cured tobacco varieties. Nine cultivars and partial diallel set of 36 F1 hybrides were grown at Taegu Experiment Station, Korea Ginseng & Tobacco Research Institute in 1983. Estimated heritability in the narrow-sense ranged from 66.21% to 94.12% for yield, leaves per plant, days to flower, leaf weight, leaf width, leaf shape, nicotine content and reducing sugar content, while that for stalk height, leaf length, midrib weight and midrib width ranged from 28.12% to 56.25%. The genotypic correlations were positive among yield, leaf weight, leaf length, leaf width, midrib weight and midrib width in the middle leaves, days to flower, nicotine content and reducing correlated with leaf length than leaf width. At the path coefficient analysis of the effect of leaf and midrib characters upon yield according to stalk position, leaf weight, leaf width and midrib width in the middle leaves, and leaf weight, and leaf width in the top leaves showed high direct effects.

• Journal of the Korean Society of Tobacco Science
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• v.11 no.2
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• pp.143-148
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• 1989

Field experiment was conducted to find out the chemical characteristics of 12 Korean local varities, Local varities were compared with oriental, flue-cured and burley tobacco. Among tested varities, Hyangcho and Matskawa were desirable in respect of flavor, leaf color and texture. Hyangcho and Matskawa were higher in the content of nicotine and petroleum ether extract. Gajacho, Byeolcho and Kukjuo were higher in the content of reducing sugar, but lower nicotine, total nitrogen and petroleum ether extract. The nicotine and total nitrogen content of Kwangcho, Daruma and Catterton were lower than that of Br. 21. In the content of volatile organic acid which were major characteristic component of oriental tobacco, the Korean local varities were lower in the content of 3-methyl butanoic and 3-methyl pentanoic acid, but hirities, Hyangcho, Matskawa and Daruma were higher in the content of volatile organic acid. Korean local varities, especially small leaf group, were higher in the content of benzyl alcohol and furfural than any other type tobacco.

• Journal of the Korean Society of Tobacco Science
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• v.17 no.2
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• pp.114-119
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• 1995

Cultivars of Nicotiana tabacum L. normally have pink flowers, but the flue-cured tobacco mutant line, BU 8832-85, had white flower. The mutant line was crossed with five normal varieties of KF 109, NC 82, TC 499, NC 567 and Coker 176. All Fl plants showed pink flower. The progenies of F2 generations were segregated with the phenotypic ratio 9 : 3 : 4 with pink, varigated(a recombinant type) and white flower, respectively. Test-cross populations showed 1 : 1 : 2 ratios. These results showed that the white flower character was controlled by two recessive genes. The genes were designated as FFCC for pink and ffcc for white flower. The recessive gene ff was epistatic to C and c. Therefore, white flower had a recessive epistasis gene.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.8 no.1
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• pp.117-119
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• 1970

The anthers of Yellow Special A, a leading tobacco variety, were cultured on the modified RM-1964 medium. Approximately three weeks after culture, plantlets emerged out of the anthers, and they reached blooming stage in six months. 24 univalent chromosomes were most frequently observed at M1 of PMC. Number of bivalent was 0.8 per PMC.

• Journal of the Korean Society of Tobacco Science
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• v.3 no.2
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• pp.115-122
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• 1981

Data for this study were obtained from Burley 21 (Nicotiana tabacum L.) grown under various densities on the field in 1978 and 1979 at the Jeonju Experiment Station, Korea Ginseng & Tobacco Research Institute. Interrelations between yield and its components were statistically studied by correlation, regression, and pathway analysis. Correlation of yield with plant population was significant and positive. Quadratic functions for yield vs. plant population and the length of the largest leaf were fitted to the data. Multiple recession equation between yield and its components (leaf number ($X_1$), a leaf area ($X_5$), weight per unit leaf area ($X_9$), plant population ($X_14$)), was significant at the 5% level. Measuring the relative importance of its components on yield, plant population was 49.5%, weight per unit leaf area 25.3%, a leaf 15.6%, and leaf number 9.8%.

• KSBB Journal
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• v.22 no.4
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• pp.185-190
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• 2007

Productivity of secreted recombinant protein depends largely on its stability in the extracellular environment with protease. Most hGM-CSF produced by transgenic tobacco cell cultures and secreted to the medium was confirmed to be rapidly degraded by protease in medium. To increase the productivity, therefore, various protein stabilizers such as gelling agents such as carrageenan and alginate, polymers, polyols, and amino acids have been tested. The stability of hGM-CSF in spent medium without cells was improved by the presence of gelling agents. However, the reason for the enhanced production by the addition of gelling agents may be due to the increased expression level and permeability rather than stability. The addition of DMSO inhibited the cell growth, but improved specific yield. The others were not effective for stability as well as hGM-CSF production.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.22 no.1
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• pp.41-44
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• 1977

Production of haploids in vitro anther culture of Nicotiana tabacum L. was oriented in a large number on a chemically defined culture medium. The haploids were screened for T.M.V. resistance and the segregating ratio in F$_1$ were in good agreement with the expected ratio.

• Journal of the Korean Society of Tobacco Science
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• v.16 no.1
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• pp.64-68
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• 1994

The present experiments were conducted to investigate some of the factors affecting the number of haploids derived from anther culture of Nicotiana tabacum. Anther - donor plants grown under controlled environment room at 3$0^{\circ}C$ yielded more haploid than room at 18, 25 and 26-22-18$^{\circ}C$ in anther culture. Donor plants starved of fertilizer yielded more haploids as compared to those of the well fed with fertilizer in anther culture. Pretreatment of exercised flower bud at 5$^{\circ}C$ was shown to be more effective in anther culture than pretreatment at 7 and 1$0^{\circ}C$, and the optimum temperature and period of pretreatment were 4 or 6 days at 5$^{\circ}C$.

• Journal of the Korean Society of Tobacco Science
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• v.16 no.1
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• pp.57-64
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• 1994

Burley tobacco (Nicotiana tabacum L.) KB 108 was developed from a single cross between KB 104 and TC 591 which was developed from a cross between Burley 49 and Tobacco Introduction 1406. It was tested for its resistance to black shank, potato virus Y(PVY), TMV and agronomic characteristics under field conditions. KB 108 has resistance to tobacco mosaic virus(TMV) and necrotic strain of potato virus Y(PVY-VN) with secreting glandular trichomes. It has also moderate resistance to black shank caused by phytophthora parasitica val. Nicotianae. KB 108 has an up-right plant growth habit similar to Burley 21. It flowers about 1-2 days later than Burley 21. The leaf width and length of KB 108 are approximately 3 cm wider and longer than those of Burley 21. The yield of KB 108 was higher 4%, nearly equal in value per kg compared to Burley 21.

• Journal of the Korean Society of Tobacco Science
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• v.6 no.1
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• pp.3-11
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• 1984

In order to obtain the basic information for the successful breeding of aromatic tobacco (Nicotiana tabacum L.), the heterosis, combining ability and gene distributions in 28 Fl hybrids by diallel crosses between Korea local and Oriental varieties were investigated. The yield were higher, stalk height, leaf length and leaf width were larger and days to flower was shorter for the Fl hybrids when compared to mid-parent. Estimates of general combining ability were significant for all characters investigated, and specific combining ability were significant for yield, stalk height, leaves Per Plant, leaf width and days to flower. In vr-wr graph, partial dominane was exhibited by all characters investigated.