• 한국동물학회:학술대회논문집
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• 한국동물학회 2003년도 한국생물과학협회 학술발표대회
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• pp.191.1-191
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• 2003

• Journal of Plant Biotechnology
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• 제2권1호
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• pp.25-28
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• 2000

The anthocyanin gene encoding flavonoid 3',5'-hydroxylase(F3,5H) was normally expressed in Nicotiana tobacco (Xanthi) plants cocultivated with Agrobacterium tumefaciens LBA4404 carrying egg plant flavonoid 3',5'-hydroxylase cDNA. Northern blot analysis showed the normal expression of F3', 5'H gene from transgenic plants. Here we found the phenotypic differences between transgenic plants and wild-type plants. The petal shape of transgenic plants showed more round shape and around petal tube area was compared to that of wild-type tobacco plants. And the petal color of transgenic plants was much lighter than that of wild-type tobacco plants.

• Molecules and Cells
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• 제20권1호
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• pp.112-118
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• 2005

It was previously shown that AtNAP1 is a plastidic SufB protein involved in Fe-S cluster assembly in Arabidopsis. In this study, we investigated the effects of depleting SufB protein from plant cells using virus-induced gene silencing (VIGS). VIGS of NbNAP1 encoding a Nicotiana benthamiana homolog of AtNAP1 resulted in a leaf yellowing phenotype. NbNAP1 was expressed ubiquitously in plant tissues with the highest level in roots. A GFP fusion protein of the N-terminal region (M1-V103) of NbNAP1 was targeted to chloroplasts. Depletion of NbNAP1 resulted in reduced numbers of chloroplasts of reduced size. Mitochondria also seemed to be affected. Despite the reduced number and size of the chloroplasts in the NbNAP1 VIGS lines, the expression of many nuclear genes encoding chloroplast-targeted proteins and chlorophyll biosynthesis genes remained unchanged.

• 한국연초학회지
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• 제9권2호
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• pp.37-47
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• 1987

This experiment was conducted to obtain basic information on breeding of flue-cured tabacco varieties . Nine cultivars and partial diallel set of 36 Fl hybrids were grown at Daegu Experiment Station, Korea Ginseng & Tobacco Research Institute in 1983. Partial dominance with high additive gene effect was observed for yield, leaves per plant , days to flower, leaf weight, nicotine and reducing sugar content. partial dominance with additive and dominant gene effect was observed for stalk height , leaf width midrib weight Overdominance with high dominant gene effect was observed for leaf length and midrib width. The directions of dominance were positive for yield, stalk height, leaf weight, leaf length, leaf width, midrib weight, midrib width and reducing sugar content, and the negative was days to flower. The estimates of effective genes were 1 for leaves per plant, 2 for stalk height, days to flower and leaf shape. 3 to 6 for leaf length, leaf width, leaf weight and midrib weight.

• 한국연초학회지
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• 제2권1호
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• pp.28-37
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• 1980

버어리種 담배에 있어서 細胞質的 雄性不稔系統을 利用한 F1雜種 利用 可能性을 究明하고자 MS Burley21$\times$Burley37外 20個 F1雜種을 育成하여 生産力 檢定試驗을 實施한 結果 MS L8$\times$Burley49, MS Kentucky12$\times$kentucky10等 4個의 F1雜種에서 現 栽培品種인 Burley21보다 수량은 2 ~11%, 품질은 1 ~5%의 증가를 보였다.

• 한국연초학회지
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• 제4권1호
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• pp.1-5
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• 1982

The metabolic enzymes, nitrate reductase, amylase and peroxidase and the Photorespiratory enzyme, Slycolate oxidise in Nicotiana tabacum varieties were studied at various growth stages. The enzyme activities of young leaves with rapid growth were different from those of old ones with stationary growth. In young leaves, activity of nitrate reductase was higher than that in mature ones and amylase activity was fairly constant in all stages. Activities of glycolate oxidise and peroxidase were found to be significantly lower in young leaves than in mature ones. Activity of glycolate oxidase in mature middle leaves was 2.45 times higher than that of young ones and inhibited by 36% when the enzyme was treated with 0.16 mM isonicotinic hydrazide.

• 한국연초학회지
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• 제2권2호
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• pp.51-57
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• 1980

담배 모자이크 바이러스(TMV)抵抗性을 가진 T.L448A외 30品種을 供試하여 germplasm의 抵抗性源에 따른 特性을 調査한 結果, 무병징 Group은 국부병반Group에 比하여 長幹, 多收, 晩生種이었으며 品質과 內容成分은 낮았다. 두 group의 諸形質間 相關關係는 開花日數와 草長(r=0.8308), 品質과 還元糖(r=0.9110) 間에 正의 有意相關關係를 보였다. 국부병반 group중 T.I.1504는 TMV抵抗性品種育成에 利用이 可能할 것으로 思料된다.

• 한국연초학회지
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• 제21권2호
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• pp.152-159
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• 1999

A CAB cDNA clone(pKGCAB) encoding the light harvesting chlorophyll a/b binding protein of the semi-shade plant, Korean ginseng(Panax ginseng C. A. Meyer) was isolated by the one-way path random sequencing of ginseng cDNA library clones and transgenic tobacco plants(Nicotiana tabacum NC82) were produced by the transformation of this ginseng CAB gene in use of Agrobacterium tumefaciens LBA4404. The CAB gene showed type 1 structure of LHCP-II, 84% similarity in nucleotide sequence and 92% in amino acid sequence to that of Nicotiana tabacum CAB40, respectively. Seed germination and initial growth of the transgenic tobacco plants transformed with the cDNA fragment were accelerated under low light intensity compared with those of normal tobacco plant, that may result from the higher light sensitivity of the transgenic plants than that of the normal.

• 한국연초학회지
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• 제6권2호
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• pp.185-189
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• 1984

To classify the inheritance of resistance to potato virus Y, crosses between susceptible flue-cured tobacco variety NC 95 and resistant variety McNair 30 were conducted. The parents, $F_1$ plants, $F_2$ populations, and haploid plants derived from anthers of $F_1$ plants were screened for a resistance of two potato virus Y strains (PVY-VB and PVY-VN) isolated in Korea. The Chi-square values for the $F_3$ populations and haploids of $F_1$ fitted 1 :3 and 1 :1 ratios of resistant to susceptible for two strains, respectively. Therefore, it was found that the resistance of McNair 30 for the potato virus Y was controlled by a single recessive gene. Moreover the resistance to two strains screened was inherited dependently.

• 한국연초학회지
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• 제11권2호
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• pp.135-141
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• 1989

The experiment was conducted to obtain basic information on breeding of flue-cured tobacco varieties. Nine cultivars and partial diallel set of 36 F1 hybrides were grown at Taegu Experiment Station, Korea Ginseng & Tobacco Research Institute in 1983. Estimated heritability in the narrow-sense ranged from 66.21% to 94.12% for yield, leaves per plant, days to flower, leaf weight, leaf width, leaf shape, nicotine content and reducing sugar content, while that for stalk height, leaf length, midrib weight and midrib width ranged from 28.12% to 56.25%. The genotypic correlations were positive among yield, leaf weight, leaf length, leaf width, midrib weight and midrib width in the middle leaves, days to flower, nicotine content and reducing correlated with leaf length than leaf width. At the path coefficient analysis of the effect of leaf and midrib characters upon yield according to stalk position, leaf weight, leaf width and midrib width in the middle leaves, and leaf weight, and leaf width in the top leaves showed high direct effects.