• Journal of Microbiology and Biotechnology
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• v.20 no.4
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• pp.693-699
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• 2010

An antiproliferative ribonuclease with a new N-terminal sequence was purified from fruiting bodies of the edible wild mushroom Russula delica in this study. This novel ribonuclease was unadsorbed on DEAE-cellulose, but absorbed on SP-Sepharose and Q-Sepharose. It had a molecular mass of 14 kDa, as judged by fast protein liquid chromatography on Superdex 75 and SDS-polyacrylamide gel electrophoresis. Its optimal pH and optimal temperature were pH 5 and $60^{\circ}C$, respectively. The ranking of its activity toward various polyhomoribonucleotides was poly C> poly G>poly A>poly U. It could inhibit proliferation of HepG2 and MCF-7 cancer cells with an $IC_50$ value of $8.6\;{\mu}M$ and $7.2\;{\mu}M$, respectively. It was devoid of antifungal and HIV-1 reverse transcriptase inhibitory activity.

• Journal of the Korean Institute of Landscape Architecture
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• v.28 no.6
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• pp.16-28
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• 2001

The purpose of this study is to arrange the terminology of the drawings in Chosun Dynasty, and to investigate the characteristics of drawings after the middle of Chos n Dynasty. This study is based on the and analysis of historic documents and drawings including drawings from China and Japan. In order to accomplish this, the drawings for the process of design were studied first, and the techniques of drawing were studied next in comparison with Japanese drawing techniques. Lastly, the technicians of drawing were studied. The results of this study were as follows; 1) Though the procedures of design Choson dynasty were not divided into planning, designing, construction and maintenance just like those of modern society, a variety of drawings in that time were used in each level. 2) The drawings in Choson Dynasty ere divided into Tohyong and Tosol, and mostly, those for design and construction were Tohy ng. 3) Based on the interpretation of Japanese drawings, several suggestions were presented to solve the questions of the drawing techniques for design and construction. 4) The technicians of drawing were the painters who were trained in a government organization in charge of royal paintings. In the future, research of this area should be continued in to deepen study on drawings as historical materials. This research provides meaningful of guidance in the analysis of historic drawing paintings.

• Journal of Korean Society for Atmospheric Environment
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• v.19 no.2
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• pp.179-189
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• 2003

The concentration of gaseous elemental mercury (Hg) was measured concurrently with relevant environmental parameters from Yang-Jae monitoring station in Seoul during Sept. 1997 to June 2002. Although data collection was disrupted for certain periods, the grand mean concentration of Hg for this five year period was found at 5.32 $\pm$ 3.53 ng m$^{-3}$ (N = 27,170). Because of short resolution of data acquisition, we were able to examine the temporal variability of Hg at varying time scale. The diurnal variability of Hg, when investigated for each of those five years, indicated consistently the dominance of nighttime over daytime. If examined at seasonal scale, Hg level was systematically higher during winter/spring than summer/fall period. The results of this short-term variability were best explained by the combined effects of such factors as meteorological conditions (formation of inversion layer and seasonal changes) and anthropogenic source processes. However, examination of long-term variation Pattern was much more complicated to explain. Thus, extension of our study is needed to diagnose the future direction in long-term trend of Hg behavior.

• Journal of Korean Society for Atmospheric Environment
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• v.10 no.1
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• pp.24-31
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• 1994

Hourly variation of gaseous nitrous acid( HNO$_2$) concentration in Seoul air was monitored from Jan. 11 to SeP. 12, Nitrous acid concentration was determined by DS/IC over nine months of observation, HNO$_2$ range from 0.04 ppb to 5.5 ppb. Gor-tex tube as gaseous HNO$_2$generator in this study is thought to be more convenient and reproducible device than previous generator. As a result of NaOH instead of Na$_2$ CO$_3$/NaHCO$_3$ solution as the IC eluent, we could obtain more stable baseline. The concentration of the NaOH eluent was 15 mM . The limit of detection(3$\sigma$) of the liquid- Phase and gas phase nitrous acid of this method are 1.1ng/$m\ell$ , 0.04 n $\ell$ / $\ell$, respectively. The precisions evaluated by 10 replicate analysis of standard solution and standard gas generated are $\pm$1.59, $\pm$2. 89% RSD, respectively. Due to the lack of standard material for air, direct assessment of the accuracy was not possible. This study was applied to the analysis of Seoul ambient air and their results are reported herein.

• Asian Journal of Atmospheric Environment
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• v.3 no.1
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• pp.42-51
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• 2009

Saturated n-dicarboxylic acids ($C_2-C_7$, $C_9$), unsaturated dicarboxylic acids (maleic, fumaric, phthalic acid), ketocarboxylic acids (pyruvic, glyoxylic acid), and dicarbonyls (glyoxal, methylglyoxal) were determined in size-segregated samples with a high-volume Andersen air sampler at a suburban site in Saitama, Japan, May 12-17 and July 24-27, 2007 and January 22-31, 2008. The seasonal average concentrations of these detected organic acids were 670 $ng/m^3$, accounting for about 4.4-5.7% (C/C) of water-soluble organic carbon (WSOC) and 2.3-3.6% (C/C) of organic carbon (OC). The most abundant species of dicarboxylic acids was oxalic acid, followed by malonic, phthalic, or succinic acids. Glyoxylic acid and methyglyoxal were most abundant ketocarboxylic acid and dicarbonyl, respectively. Seasonal differences, size-segregated concentrations, and the correlations of these acids with ambient temperatures, oxidants, elemental carbon (EC), OC, WSOC, and ionic components were also discussed in terms of their corresponding sources and possible secondary formation pathways. The results suggested that photochemical reactions contributed more to the formation of particulate organic acids in Saitama suburban areas than did direct emissions from anthropogenic and natural sources. However, direct emissions of vehicles were also important sources of several organic acids in particles, such as phthalic and adipic acids, especially in winter.

• Herbal Formula Science
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• v.25 no.2
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• pp.145-154
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• 2017

Objectives : Bufalin is one of the bioactive component of 'Sum Su (蟾酥)', which is obtained from the skin and parotid venom gland of toad. Bufalin has been known to possess the inhibitory effects on cell proliferation and inducing apoptosis in various cancer cells. The tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) has concerned, because it can selectively induce apoptotic cell death in many types of malignant cells, while it is relatively non-toxic to normal cells. Here, we investigated whether bufalin can trigger TRAIL-induced apoptotic cell death in EJ human bladder cancer cells. Methods : Effects on the cell viability and apoptotic activity were quantified using MTT assay and flow cytometry analysis, respectively. To investigate the morphological change of nucleus, DAPI staining was performed. Protein expressions were measured by immunoblotting. Results : A combined treatment with bufalin (10 nM) and TRAIL (50 ng/ml) significantly promoted TRAIL-mediated growth inhibition and apoptosis in EJ cells. The apoptotic effects were associated with the up-regulation of death receptor proteins, and the down-regulation of cFLIP and XIAP. Moreover, our data showed that bufalin and TRAIL combination activated caspases and subsequently increased degradation of poly(ADP-ribose) polymerase. Conclusions : Taken altogether, the nontoxic doses of bufalin sensitized TRAIL-mediated apoptosis in EJ cells. Therefore, bufalin might be an effective therapeutic strategy for the safe treatment of TRAIL-resistant bladder cancers.

• Environmental Analysis Health and Toxicology
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• v.16 no.4
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• pp.181-188
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• 2001

A gas chromatographic method was established for the simultaneous determination of urinary nicotine and cotinine. The analytes in basified urine containing a sufficient amount of Na$_2$S0$_4$were extracted into dichloromethane by vigorous shaking. Into the transferred organic phase was added a small amount of acidified methanol (0.5 N HCI in methanol), followed by concentrating the mixture to dryness using a mild stream of nitrogen gas. The concentrate was reconstituted with methanol and the final solution analyzed using the gas chromatograph equipped with the nitrogen-phosphorus detector. The reproducibility tests showed coefficients of variation less than 11％ for both compounds. The percent recovery for both analytes ranged from 88 to 103％. The estimated method detection limits for nicotine and cotinine were 0.60 and 5.1 ng/mL, respectively. Extraction efficiencies for both nicotine and cotinine apparently declined without the addition of Na$_2$S0$_4$into the urine. Moreover, the absence of methanolic HCI in the extract resulted in almost complete evaporation of nicotine and partial loss of cotinine during the concentration process, indicating that the formation of nicotine-HCI and cotinine-HCI species is prerequisite to the suppression of the loss of both compounds.

• Journal of Korean Library and Information Science Society
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• v.3
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• pp.1-31
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• 1976

If Y'ational developments are depend on the dex-elopment of regional >ociety and de~.elopment of regional society are rely on intellcctual delrelopment of members of regional society, going abreas! oi economic capacity. Estnhlis!l:r,ent of libraries as a treasure-house of mental resources and its Jet-elopment are inevit-able and important. But in every respect :f modern libraries are not equipped with contemporary devclij;!~i:c:\ulcornert of society. One c~i the resolving problems, like this, opening the door of the liI~-,\ulcornerr:~fa-c ilities, as one object, which located in every regional areas, u.9ng the existing facilities, the width of the library services mus: I)c opened. I-' ;- I n,> study is aimed at like the following: 1. is analysizing the situation ~vhich the library facilities of one ti:u~:sa!~d five hundred and fifty nine located here and there ir, Ii.;~-ui:gij~opkr ovince in Korea and s i s t ~t.l lousand seven hundred aid tliirl:~ six village libraries, for connecting as a library networks. 2. Tl\ulcornerc ivhole libraries in Kyungbook province and dividing into four ::arts as a Daegu, Phohang, Ahndong and Kimchun area as a transportation area, school area and resources area which exhibiting the view-point of helping-net~vorksl ike the following,

• Journal of information and communication convergence engineering
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• v.8 no.3
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• pp.328-332
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• 2010

Filipino language is an Austronesian language based on numerous native languages with influences from other major languages such as English and Spanish. The Filipino alphabet is consists of 26 English alphabets, with the addition of two letters, "$\tilde{n}$" and "ng", a total of 28 letters. Filipino language expressions and sentences are still incomparable to English and Spanish even though there are numerous borrowed words from these languages. This study aims to discover the uniqueness in the Filipino language by identifying the frequencies of the letters in common words used and be able to introduce a revolutionary keypad for the Filipinos which is scientifically efficient. To compare the efficiency of the revised and the original keypad, computations using Fitts' Law, Hick-Hyman Law and KSPC were done. Results showed that the new keypad layout was more effective than the original keypad. Introducing a improvised keypad to the Filipinos is one step closer in achieving customized services and features to Filipinos in mobile computing.

• Herbal Formula Science
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• v.17 no.1
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• pp.17-28
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• 2009

Objectives : The result is the followings after investingating composition, dosage and usage of Yukmijihwang-Hwan(六味地黃丸) in literature by country and Era. Methods : Investigated Classics of Oriental Medicine that Yukmijihwang-Hwan is first described. Results : Found 42 literatures searching Yukmijihwang-Hwan in Classics of Oriental Medicine. Literature that Yukmijihwang-Hwan is recorded by first was Yinhaijingwei(銀海精微), but capacity has not recorded. Yukmijihwang-Hwan Medicinal Decoction that eat(Yukmijihwang-Tang: 5 times) as pill than eat(Yukmijihwang-Hwan: 37 times) was more. Literature that composition of Yukmijihwang-Hwan is recorded by Rehmannia glutinosa(熟地黃) is 8 Ryang(兩), Poria cocos(白茯苓) is 3 Ryang, Paeonia suffruticosa(牧丹皮) is 3 Ryang, Alisma Canaliculatum (澤瀉) 3 Ryang, Cornus officinalis(山茱萸) 4 Ryang, Dioscorea batatus(山藥) 4 Ryang was most. Conclusions : Yukmijihwang-Hwan wat difference in composition and capacity that Chinese and Korean Classics of Oriental Medicine. After Dongeuibogam of the Korea is published, composition and capacity are connected until today.