• Journal of Microbiology and Biotechnology
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• v.33 no.5
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• pp.644-655
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• 2023

Safety assessment and functional analysis of probiotic candidates are important for their industrial applications. Lactiplantibacillus plantarum is one of the most widely recognized probiotic strains. In this study we aimed to determine the functional genes of L. plantarum LRCC5310, isolated from kimchi, using next-generation, whole-genome sequencing analysis. Genes were annotated using the Rapid Annotations using Subsystems Technology (RAST) server and the National Center for Biotechnology Information (NCBI) pipelines to establish the strain's probiotic potential. Phylogenetic analysis of L. plantarum LRCC5310 and related strains showed that LRCC5310 belonged to L. plantarum. However, comparative analysis revealed genetic differences between L. plantarum strains. Carbon metabolic pathway analysis based on the Kyoto Encyclopedia of Genes and Genomes database showed that L. plantarum LRCC5310 is a homofermentative bacterium. Furthermore, gene annotation results indicated that the L. plantarum LRCC5310 genome encodes an almost complete vitamin B₆ biosynthetic pathway. Among five L. plantarum strains, including L. plantarum ATCC 14917^T , L. plantarum LRCC5310 detected the highest concentration of pyridoxal 5'-phosphate with 88.08 ± 0.67 nM in MRS broth. These results indicated that L. plantarum LRCC5310 could be used as a functional probiotic for vitamin B₆ supplementation.

• The KIPS Transactions:PartC
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• v.11C no.7 s.96
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• pp.1023-1032
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• 2004

It is generally known that handoff delay degrades the QoS by packet drop, packet delay and jitter. Moreover. handoff highly effects on QoS in beyond 3G system because not only micro cells for achieving high capacity and fast moving nodes induce the frequent handoff but also hard handoff must be carried out in OFDM system. Therefore, study on a handoff algorithm for guaranteeing QoS is required. Related works on handoff for beyond 3G system mainly focused on reducing the L3 handoff delay or packet loss. That is, these schemes try to compensate L2 delay rather directly eliminate it. However, remained 1.2 delay degrades QoS, especially delay-sensitive realtime traffic. Therefore, we proposed the seamless handoff algorithmwhich can minimize the L2 handoff delay.

• Animal cells and systems
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• v.1 no.3
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• pp.467-473
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• 1997

Taurine (2-aminoethanesulfonic acid), one of bioactive amino acid in the mammalian brain, is known to exert inhibitory effects on neurons via GABA receptor. In the present study, we examined effects of taurine on glutamateinduced neurotoxicity on hippocampal neuron cell culture using cell counting method and lactate dehydrogenase (LDH) assay. After 10 d of culture, cells were stimulated with appropriate drugs. Only 43% of cultured neuronal cells survived at one day after stimulation with 500 uM L-glutamate for 10 min. Survival rate was enhanced by 82% in the presence of 10 mM taurine. LDH activity from the culture supernatant incubated with a combination of L-glutamate and taurine was less than half of that with L-glutamate alone. In the next series of experiments, interleukin-6 (IL-6) mRNA expression in cultured astrocytes was investigated using reverse tanscription-PCR (RT-PCR). IL-6 mRNA was detected in the astrocytes stimulated with L-glutamate in a dose-dependent manner, while not detected in the unstimulated control astrocytes. The expression of IL-6 mRNA caused by 10 mM glutamate was inhibited by taurine, but not by GABA. These findings demonstrated a neuroprotective action of taurine against glutamate-induced toxicity.

• Environmental Engineering Research
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• v.13 no.4
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• pp.216-223
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• 2008

An efficient method for the simultaneous determination of eleven phenolic endocrine-disrupting chemicals (EDCs) present in wastewater influent samples was described. The 11 phenolic EDCs including alkylphenols, chlorophenols, and bisphenol A were determined by gas chromatography/mass spectrometry-selected ion monitoring (GC/MS-SIM) following two work-up methods for comparison; isobutoxycarbonyl (isoBOC) derivatization and tert-butyldimethylsilyl (TBDMS) derivatization. The wastewater influent samples containing the 11 EDCs were adjusted to pH 2 with $H_2SO_4$ and then cleaned up with n-hexane. Next, they were subjected to solid-phase extraction (SPE) with XAD-4 resin and subsequently converted to isoBOC or TBDMS derivatives for sensitivity analysis with gas chromatography/mass spectrometry-selected ion monitoring (GC/MSSIM). Following isoBOC derivatization and TBDMS derivatization, the recoveries were 86.6-105.2% and 97.6-142.7%, the limits of quantitation (LOQ) for the 11 phenolic EDCs for SIM was 0.001-0.050 ng/mL and 0.003-0.050 ng/mL, and the SIM responses were linear with the correlation coefficient varying by 0.9717-0.9995 and 0.9842-0.9980, respectively. When these methods were applied to five selected wastewater influent samples, for isoBOC derivatization and TBDMS derivatization the ranges of concentration detected were 0.2-99.6 ng/mL and 0.4-147.4 ng/mL, respectively.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.9 no.12
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• pp.5103-5115
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• 2015

In radar imaging, a target is usually consisted of a few strong scatterers which are sparsely distributed. In this paper, an improved sparse signal recovery algorithm based on smoothed l₀ (SL0) norm method is proposed to achieve high resolution ISAR imaging with limited pulse numbers. Firstly, one new smoothed function is proposed to approximate the l₀ norm to measure the sparsity. Then a single loop step is used instead of two loop layers in SL0 method which increases the searching density of variable parameter to ensure the recovery accuracy without increasing computation amount, the cost function is undated in every loop for the next loop until the termination is satisfied. Finally, the new set of solution is projected into the feasible set. Simulation results show that the proposed algorithm is superior to the several popular methods both in terms of the reconstruction performance and computation time. Real data ISAR imaging obtained by the proposed algorithm is competitive to several other methods.

• Journal of Microbiology and Biotechnology
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• v.23 no.3
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• pp.357-363
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• 2013

The identification of bacteriophage proteins on the surface of Lactobacillus rhamnosus Pen was performed by LC-MS/MS analysis. Among the identified proteins, we found a phage-derived major tail protein, two major head proteins, a portal protein, and a host specificity protein. Electron microscopy of a cell surface extract revealed the presence of phage particles in the analyzed samples. The partial sequence of genes encoding the major tail protein for all tested L. rhamnosus strains was determined with specific primers designed in this study. Next, RT-PCR analysis allowed detection of the expression of the major tail protein gene in L. rhamnosus strain Pen at all stages of bacterial growth. The transcription of genes encoding the major tail protein was also proved for other L. rhamnosus strains used in this study. The present work demonstrates the spontanous release of prophage-encoded particles by a commercial probiotic L. rhamnosus strain, which did not significantly affect the bacterial growth of the analyzed strain.

• Journal of the Korean Society of Costume
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• v.59 no.8
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• pp.66-81
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• 2009

The purposes of this study were to analyze the formative relationship between Surrealistic clothes and hat with the focus on the period from the 1990's to 2008 and to find out the development direction of next hat design. The method to analyze the relationship between clothes and hats was the formative analysis by Marian L. Davis and Marilyn R. Delong. Hat design of surrealism was cylindrical or tear drop in a shape according to the principle of $D{\acute{e}}paysement$ and Trompe l'oeil, and was similar with the Form of a jacket, a sleeve, or a skirt. In color, accent color coordination mainly appeared and sometimes contrast color coordination induced illusion effect. In material, a variety of materials were used according to the principle of $D{\acute{e}}paysement$ and Trompe l'oeil, and contrast was widely used like color. In Decoration, the decoration or pattern of clothes was intactly applied to hats. The results suggest that clothes and hat have an organic relationship, and a hat style is changed with formative elements of clothes.

• Steel and Composite Structures
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• v.5 no.5
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• pp.359-374
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• 2005

This paper presents the experimental and theoretical results of the viscoplastic response and collapse of 316L stainless steel tubes subjected to cyclic bending. The tube bending machine and curvature-ovalization measurement apparatus, which was designed by Pan et al. (1998), were used for conducting the cyclic curvature-controlled experiment. Three different curvature-rates were controlled to highlight the characteristic of viscoplastic response and collapse. Next, the endochronic theory and the principle of virtual work were used to simulate the viscoplastic response of 316L stainless steel tubes under cyclic bending. In addition, a proposed theoretical formulation (Lee and Pan 2001) was used to simulate the relationship between the controlled cyclic curvature and the number of cycles to produce buckling under cyclic bending at different curvature-rates (viscoplastic collapse). It has been shown that the theoretical simulations of the response and collapse correlate well with the experimental data.

• Proceedings of the KIEE Conference
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• 1995.07a
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• pp.71-73
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• 1995

For the accurate analysis of induction motors driven by PWM-type inverter, a time-stepping finite element method is presented in this paper. Since the PWM-type source voltage is not sinusoidal, the time harmonic method can not be used. Therefore, we used a time-stepping method, where the space harmonics due to the slot structure can be analyzed and each time-step size is determined from each increase of rotor position. As a numerical example, an induction motor of 20 Hp, 3 phase and 6 pole is analyzed. First, numerical results of the time-stepping finite element analysis are compared to those of conventional equivated circuit analysts. Next, the stator current characteristic obtained from PWM voltage source is compared to that from sinusoidal voltage source.

• Journal of the Korea Society of Computer and Information
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• v.23 no.1
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• pp.91-102
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• 2018

The purpose of this study is to develop an excellent bodice prototype that is adapted to the body shape of middle-aged women using 3D measurement data. In the evaluation of appearance, S pattern 4.00, B pattern 2.80, E pattern 2.40, L pattern 1.40 were shown in order, and the best fit of S pattern was evaluated as excellent. As a result of looking at the color distribution chart to find out the amount, E pattern and S pattern were not space in the front bust, armhole, and the back waist line. The B pattern and the L pattern were marked in blue because of insufficiency space in the back neck. As a result of evaluation the amount of air gap in the clothing, the air gap of the bust was 0.12, which is the largest pattern of B. Next, the L pattern appears as a tight circle with smallest air gap in the order of the S pattern 0.096, the E pattern 0.08, and the L pattern 0.003. The S pattern was evaluated to be the most appropriate for the body shape of middle-aged women. But the waist and back were slightly tight. Middle-aged women have larger shoulder-related items and larger waist circumference. Therefore, when you set the perimeter item, you should add 1-2cm of space amount and give extra space to the circumference area.