• Title/Summary/Keyword: Next Generation Sequence

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A Fault Section Detection Method for Ungrounded System Based on Phase Angle Comparison of Zero-Sequence Current (비접지 배전계통에서 영상전류 위상 비교에 의한 고장구간 검출 방법)

  • Yang, Xia;Choi, Myeon-Song;Lee, Seung-Jae
    • Proceedings of the KIEE Conference
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    • 2007.07a
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    • pp.31-32
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    • 2007
  • In this paper, a fault section detection method is proposed for ungrounded system in the case of a single line-to-ground fault. A conventional method is used for faulted feeder selection according to the angular relationship between zero-sequence currents of the feeders and zero-sequence voltage of the system. Fault section detection is based on the comparison of phase angle of zero-sequence current. Proposed method has been testified in a demo system by Matlab/Simulink simulations. Based on Distribution Automation System(DAS), Feeder Remote Terminal Unit(FRTU) is used to collect those necessary data, at present a demo system is under developing using Manufacturing Message Specification (MMS) in IEC61850 standard.

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A Restoration Algorithm using Moving the tie Switch in Distribution System in case of Fault Occur in Power system Substation MTR (변전소 내 주변압기 사고 발생 시 배전계통 연계점 이동을 통한 복구 알고리즘)

  • Hong, Jun-Ho;Lee, Seung-Jae;Choi, Myeon-Song;Lim, Il-Hyung;Kim, Tae-Wan
    • Proceedings of the KIEE Conference
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    • 2009.07a
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    • pp.159_160
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    • 2009
  • In this paper proposes a new algorithm of efficiency outage restoration using a outage load switching to a healthy MTR a fault occurrence at a MTR. In addtion, proposed algorithm includes a outage restoration method which keeps MTR optimal capacity with reorganization of distribution network in case it can not restore outage state loads caused by shortage of healthy MTR remain capacity. In case that proposed sequence still can not complete restoration, this paper suggests a efficiency outage restoration with objective function included priority in outage loads.

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Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China

  • Zhao, Ling;Zhang, Liang;Shi, Xiaona;Duan, Xujie;Li, Huiping;Liu, Shuying
    • Journal of Veterinary Science
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    • v.22 no.6
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    • pp.66.1-66.9
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    • 2021
  • Background: Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide. Objectives: In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated. Methods: Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis. Results: A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%-86.8% and 67.7%-75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5). Conclusions: The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.

A Modified Selective Ground Relay for Ungrounded Distribution Systems

  • Nam Soon-Ryul;Kang Sang-Hee;Park Jong-Keun
    • Journal of Electrical Engineering and Technology
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    • v.1 no.1
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    • pp.16-22
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    • 2006
  • Selective ground relays (SGRs) are useful fur distinguishing a faulted feeder from the sound feeders in ungrounded systems. However, they sometimes mis-operate due to human or device errors. Particularly, the reversed polarity of zero-sequence current transducers (ZCTs) is the most frequent cause of mis-operation. This paper presents a modified SGR for reducing the probability of mis-operations caused by the reversed polarity of ZCTs. The modification is achieved by introducing an adaptive time delay, which depends on the magnitude of the zero-sequence current and the phase angle deviation from the reference. The modified SGR was successfully demonstrated on a sample ungrounded system without mis-operation.

A Simple GUI-based Sequencing Format Conversion Tool for the Three NGS Platforms

  • Rhie, A-Rang;Yang, San-Duk;Lee, Kyung-Eun;Thong, Chin Ting;Park, Hyun-Seok
    • Genomics & Informatics
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    • v.8 no.2
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    • pp.97-99
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    • 2010
  • To allow for a quick conversion of the proprietary sequence data from various sequencing platforms, sequence format conversion toolkits are required that can be easily integrated into workflow systems. In this respect, a format conversion tool, as well as quality conversion tool would be the minimum requirements to integrate reads from different platforms. We have developed the Pyrus NGS Sequencing Format Converter, a simple software toolkit which allows to convert three kinds of Next Generation Sequencing reads, into commonly used fasta or fastq formats. The converter modules are all implemented, uniformly, in Java GUI modules that can be integrated in software applications for displaying the data content in the same format.

A Primer for Disease Gene Prioritization Using Next-Generation Sequencing Data

  • Wang, Shuoguo;Xing, Jinchuan
    • Genomics & Informatics
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    • v.11 no.4
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    • pp.191-199
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    • 2013
  • High-throughput next-generation sequencing (NGS) technology produces a tremendous amount of raw sequence data. The challenges for researchers are to process the raw data, to map the sequences to genome, to discover variants that are different from the reference genome, and to prioritize/rank the variants for the question of interest. The recent development of many computational algorithms and programs has vastly improved the ability to translate sequence data into valuable information for disease gene identification. However, the NGS data analysis is complex and could be overwhelming for researchers who are not familiar with the process. Here, we outline the analysis pipeline and describe some of the most commonly used principles and tools for analyzing NGS data for disease gene identification.

First complete mitogenome sequence of Korean Gloydius ussuriensis (Viperidae: Crotalinae)

  • Hye Sook Jeon;Min Seock Do;Jung A Kim;Yoonjee Hong;Chae Eun Lim;Jae-Hwa Suh;Junghwa An
    • Journal of Species Research
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    • v.13 no.2
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    • pp.127-130
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    • 2024
  • The first complete mitogenome sequence of the Red-tongue Pit Viper (Gloydius ussuriensis) from Korea was characterized using next-generation sequencing. The mitogenome is a circular molecule (17,209 bp) with a typical vertebrate mitogenome arrangement, which consists of 2 ribosomal RNA genes (rRNA), 22 transfer RNA genes (tRNA), two non-coding regions (D-loop), and 13 protein-coding genes (PCGs). The base composition of the mitogenome is 32.7% of A, 27.5% of C, 13.9% of G, and 25.9% of T, with a slight AT bias(58.6%). This phylogenetic analysis infers that G. ussuriensis is in the same group as the Chinese G. ussuriensis (Accession No. KP262412) and is closely related to G. blomhoffi and other species of the genus Gloydius. In our study, the complete mitogenome sequence of Korean G. ussuriensis was characterized and we provided basic genetic information on this species.

Assembly sequence generation using genetic algorithm (유전자 알고리즘을 이용한 조립순서 추론)

  • 홍대선;조형석
    • 제어로봇시스템학회:학술대회논문집
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    • 1997.10a
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    • pp.1267-1270
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    • 1997
  • An assembly sequence is considered to be optimal when it minimizes assembly cost while satisfying assembly constraints. to generate such sequences for robotic assembly, this paper proposes a method using a genetic algorithm (GA). This method denotes an assembly sequence as an individual, which is assigned a fitness related to the assembly cost. Then, a population consisting of a number of individuals evolves to the next generation through genetic operations of crossover and mutation based upon the fitness of the individuals. The population continues to repetitively evolve, and finally the fittest individual and its corresponding assembly sequence is found. Through case study for an electrical relay, the effectiveness of the proposed method is demonstrated. Also, the performance is evaluated by-comparing with those of previously presented approaches such as a neural-netowork-based method and a simulated annealing method.

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Next Generation Sequencing (NGS), A Key Tool to open the Personalized Medicine Era

  • Kwon, Sun-Il
    • Korean Journal of Clinical Laboratory Science
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    • v.44 no.4
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    • pp.167-177
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    • 2012
  • Next-Generation Sequencing (NGS) is a term that means post-Sanger sequencing methods with high-throughput sequencing technologies. NGS parallelizes the sequencing process, producing thousands or millions of sequences at once. The latest NGS technologies use even single DNA molecule as a template and measures the DNA sequence directly via measuring electronic signals from the extension or degradation of DNA. NGS is making big impacts on biomedical research, molecular diagnosis and personalized medicine. The hospitals are rapidly adopting the use of NGS to help to patients understand treatment with sequencing data. As NGS equipments are getting smaller and affordable, many hospitals are in the process of setting up NGS platforms. In this review, the progress of NGS technology development and action mechanisms of representative NGS equipments of each generation were discussed. The key technological advances in the commercialized platforms were presented. As NGS platforms are a great concern in the healthcare area, the latest trend in the use of NGS and the prospect of NGS in the future in diagnosis and personalized medicine were also discussed.

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Whole Genome Sequence of Streptomyces sp. from Novel Marine Actinomycetes

  • Hyeon Kyeong Lee;Heung-Soon Park;Eung-Soo Kim;Si-Sun Choi
    • Microbiology and Biotechnology Letters
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    • v.51 no.3
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    • pp.325-327
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    • 2023
  • This paper presents the complete genome sequence of a novel marine actinomycete, Streptomyces sp. MMBL 11-1. The genome of Streptomyces sp. MMBL 11-1 was obtained through next-generation sequencing using the PacBio Sequel system and Illumina platform provided by Macrogen, Korea. The assembled genome consists of five contigs, with a total length of 8,496,900 bp and a G+C content of 71.6%. The genome harbors multiple biosynthetic gene clusters (BGCs) associated with producing microbial natural products (MNPs). The comprehensive genomic information of this type of strain will serve as a valuable resource for identifying other marine actinomycetes strains.