• 대한수의학회지
• /
• 제55권2호
• /
• pp.89-95
• /
• 2015

Johne's disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is one of the most widespread and economically important diseases in cattle. Current diagnostic methods are based on the detection of anti-MAP antibodies in serum or isolation of the causative agent. However, these techniques are often not applicable for cases of subclinical infection due to relatively low sensitivity. Therefore, finding new antigen candidates that strongly react with the host immune system had been attempted. To effectively detect infection during the subclinical stage, several antigen candidates were selected based on previous researches. Characteristics of the selected antigen candidates were analyzed using bioinformatics-based prediction tools. A total of nine antigens were selected (MAP0862, MAP3817c, MAP2077c, MAP0860c, MAP3954, MAP3155c, MAP1204, MAP1087, and MAP2963c) that have MAP-specific and/or high immune responses to infected animals. Using a transmembrane prediction tool, five of the nine antigen candidates were predicted to be membrane protein (MAP3817c, MAP3954, MAP3155c, MAP1087, and MAP1204). Some of the predicted protein structures identified using the I-TASSER server shared similarities with known proteins found in the Protein Data Bank database (MAP0862, MAP1204, and MAP2077c). In future studies, the characteristics and diagnostic efficiency of the selected antigen candidates will be evaluated.

• 한국미생물학회:학술대회논문집
• /
• 한국미생물학회 2003년도 International Meeting of the Microbiological Society of Korea
• /
• pp.83-86
• /
• 2003

Major advances in positive-sense RNA virus research have been facilitated by the development of reverse genetics systems. These systems consist of an infectious cDNA clone that encompasses the genome of the virus in question. This clone is then used as a template for the subsequent synthesis of infectious RNA for the generation of synthetic viruses. However, the construction of infectious cDNA for the Japanese encephalitis virus (JEV) has been repeatedly thwarted by the instability of its cDNA. As JEV is an important human pathogen that causes permanent neuropsychiatric sequelae and even fatal disease, a reliable reverse genetics system for this virus is highly desirable. The availability of this tool would greatly and the development of effective vaccines as well as facilitate studies into the basic biology of the virus, including the molecular mechanisms of viral replication, neurovirulence, and pathogenesis. We have successfully constructed a genetically stable infectious JEV cDNA containing full-length viral RNA genome. Synthetic RNA transcripts generated in vitro from the cDNA were highly infectious upon transfection into susceptible cells, and the cDNA remained stable after it had been propagated in E. coli for 180 generations. Using this infectious JEV cDNA, we have successfully expressed a variety of reporter genes from the full-length genomic and various subgenomic RNAs in vitro transcribed from functional JEV cDNAS. In summary, we have developed a reverse genetics system for JEV that will greatly facilitate the research on this virus in a variety of different fields. It will also be useful as a heterologous gene expression vector and aid the development of a vaccine against JEV.

• 한국산학기술학회논문지
• /
• 제22권2호
• /
• pp.231-239
• /
• 2021

본 연구는 구조방정식을 통하여 학생들의 치과 위생사에 대한 이미지와 취업 인식을 파악하고 온라인 정보에 대한 인식을 접목해 차후 치과위생사 보건인력 확보에 필요한 정보를 마련하고자 한다. 수집된 자료는 통계는 SPSS Statistics 24.0 와 AMOS Graphics 21.0 통계 패키지를 이용하여 자료 분석을 시행하였다. 취업을 긍정적으로 인식하는 사람은 신종 감염병이 발생해도 치과위생사의 이미지를 긍정적으로 보고, 치과위생사의 부정적 이미지를 통해 부정적 취업 인식을 이해하는 것으로 나타났다. 인터넷 정보를 긍정적으로 생각하는 사람은 인터넷 정보가 거짓 정보가 많고, 유해하며, 불안을 조장한다고 생각하고, 그 영향은 취업에 대한 부정적 인식에 영향을 미치는 것으로 나타났다. 인터넷 정보를 긍정적으로 생각하는 사람은 치과위생사가 감염에 노출되기 쉽고, 감염원을 전달 할 수 있으며, 쉽게 감염될 수 있다고 생각하고, 이는 부정적 취업에 영향을 미치는 것으로 나타났다. 결론적으로 신종 감염병 시기에는 올바른 인터넷 정보의 인식을 통하여 치과위생사의 이미지 형성을 하는 것이 중요하다.

• 산업융합연구
• /
• 제21권2호
• /
• pp.111-116
• /
• 2023

최근 바이러스 감염병 및 새로운 질병은 한 지역에 국한되는 것이 아니라 전 세계적으로 확산되어 경제적, 사회적으로 심각한 피해를 일으키고 있다. 또한, 새로운 질병의 발현 주기가 짧고, 확산 속도도 빨라지고 있다. 질병에 대한 확산을 막기 위해 격리, 폐쇄 등 질병 발생 후 수동적인 형태의 대응이 우선시 되고 있다. 이러한 형태의 대응은 질병 확산 방지에 근본적인 대응으로는 부족한 부분이 많이 있다. 이에, 본 연구에서는 새로운 질병 발생 정보를 포함한 질병 예방 모니터링 시스템을 제안한다. 본 연구에서는 IoMT 환경 구축을 통해 질병 정보와 사용자의 정보를 수집한다. devices를 이용한 정보 수집은 사용자의 생체정보와 질병 서버에 등록된 데이터를 수집하고 분류한다. IoMT 환경에서는 사용자의 데이터를 수집하여 사용자 질병 감염 여부를 평가하여 사용자에게 제공한다. 본 연구를 통해 개인의 질병 증상 정보를 제공하고, 질병 확산에 대한 능동적인 대응 방법을 제공할 수 있다.

• 한국가금학회지
• /
• 제38권3호
• /
• pp.181-189
• /
• 2011

새 깔짚을 사용하는 16개 농가와 깔짚을 교체하지 않는 15개 농가를 대상으로 2009년 7월부터 11월까지 5개월에 걸쳐 농가당 2회 입식 기준으로 병아리 입식 전과 출하 전 깔짚을 수거하여 주요 바이러스 병원체의 오염 실태를 조사하고, 생산성을 분석하였다. 그 결과, 조사 기간 동안 깔짚을 교체해 주는 농가의 75%(12/16농가)에서, 깔짚을 재사용하는 농가의 73%(11/15)에서 37주의 바이러스가 분리되었다. 바이러스분리 시기별로보면병아리 입식 직전계사에서3주, 육계 출하 직전 계사에서 34주가 분리되었다. 깔짚 형태로 보면 새 깔짚을 사용하는 계사에서 17주, 깔짚을 재사용하는 계사에서 20주가 분리되어 계사 내 바이러스 병원체 오염정도는 깔짚 교체여부와 크게 관련성이 없는 것으로 나타났다. 분리된 바이러스 37주는 병원체별로 보면 IBV 14주, IBDV 2주, FAdV 9주 그리고 CIAV 12주였다. 이 중 IBDV 1주와 CIAV 2주는 입식 직전 계사에서 분리되었다. 본 연구에서 조사한 31개 농장 62개 계군의 생산지수를 조사한 결과, 깔짚 교체 농가와 재활용 농가 간 생산성은 유의성이 있는 차이가 나타나지 않았다.

• 한국동물학회지
• /
• 제2권1호
• /
• pp.17-28
• /
• 1959

In Korea ,little attention was paid to chiggers until 1950 so only to reports on four species of chiggers were pulished before Korean War. Since 1950 (beginning of Korean War), a marked progress in the study of chiggers has been made in connection with investigations of Epidemic hemorrhagic fever occurring among the United Nations troops which was suspected as a chigger-borne infectious disease and the first report of tsutsugamushi disease in Korea called natives attention to chiggers as the vector mites of this newly known disease in Korea. This paper is mostly based on specimens of author's collections from 3269 Rodentia Insectivora, 9 Chiroptera, 24 Aves , 35 Amphibia and 3 Reptilia during the time from December 1955 to December 1956 but four species were introduced here from works of others . There are reported here five species of chiggers previously known only out of Korea and a new species also. This new species was collected by author but Lipovsky informed his colleague had collected the same one in Korea and they would publish it as new one in near future. This is the reason of describing the new species without specific name . Of course, this paper is incomplete in view of the geographic distribution, seosonal change and host relation etc, but will serve as a brief summary of the chiggers fauna of Korea up to the present. This species described here are as follows : Gahrliepia brennani var. ventralis Neoschongastia posekanyi Euschongastia kigtajimai Euschongastia miyagawai Euschongastia koreaensis Trombicula nagayoi Trombicula japonica Trombicula pomeranzevi Trombicula mitamurai Trombicula tamiyai Trombicula palpalis Trombicula orientalis Trombicula pallida Trombicula scutellaris Trobmicula miotis Trombicula hiranumai Trombicula sp. Trombicula hiranumai Trombicula sp. Trombicula subintermedius Shunsennia tarsalis . Euschongastia ikaoensis . Trombicula koomori Trombicul subakanushi

• Journal of Veterinary Science
• /
• 제21권4호
• /
• pp.63.1-63.9
• /
• 2020

Background: Canine adenovirus type 2 (CAV-2) induces infectious laryngotracheitis in members of the family Canidae, including dogs. To date, no ELISA kits specific for CAV-2 antibody have been commercialized for dogs in Korea. Objectives: We aimed to develop new indirect enzyme-linked immunosorbent assay (I-ELISA) to perform rapid, accurate serological surveys of CAV-2 in dog serum samples. Methods: In total, 165 serum samples were collected from dogs residing in Chungbuk and Gyeongbuk provinces between 2016 and 2018. The Korean CAV-2, named the APQA1701-40P strain, was propagated in Madin-Darby canine kidney cells and purified in an anion-exchange chromatography column for use as an antigen for I-ELISA. The virus-neutralizing antibody titers of CAV-2 in the dog sera were measured by virus neutralization (VN) test. Results: We compared the results obtained between the VN and new I-ELISA tests. The sensitivity, specificity, and accuracy of new I-ELISA were 98.6%, 86.4% and 97.0% compared with VN test, respectively. New I-ELISA was significantly correlated with VN (r = 0.91). Conclusions: These results indicate that new I-ELISA is useful for sero-surveillance of CAV-2 in dog serum.

• 대한치과의료관리학회지
• /
• 제6권1호
• /
• pp.1-10
• /
• 2018

Prior to the end of the 20th century, microorganism research was limited to culture and has since been revolutionized by genetic analysis. Microorganisms, including bacteria, can cause disease, but most of them are commensal microorganisms in our bodies. This knowledge changes the pathological approach to infectious diseases and lends to a new perspective on the effects of gut and oral microorganisms on disease and health. The oral cavity, particularly the periodontal pocket, is considered to be a reservoir of microbes that cause disease, and oral microbial control is becoming more important. In this review, I will examine the changes in the microbiological revolution and the meaning of oral healthcare management based on those changes.

• Journal of Veterinary Science
• /
• 제22권1호
• /
• pp.4.1-4.13
• /
• 2021

Background: Microsporum canis is a zoonotic disease that can cause dermatophytosis in animals and humans. Objectives: In clinical practice, ketoconazole (KTZ) and other imidazole drugs are commonly used to treat M. canis infection, but its molecular mechanism is not completely understood. The antifungal mechanism of KTZ needs to be studied in detail. Methods: In this study, one strain of fungi was isolated from a canine suffering with clinical dermatosis and confirmed as M. canis by morphological observation and sequencing analysis. The clinically isolated M. canis was treated with KTZ and transcriptome sequencing was performed to identify differentially expressed genes in M. canis exposed to KTZ compared with those unexposed thereto. Results: At half-inhibitory concentration (½MIC), compared with the control group, 453 genes were significantly up-regulated and 326 genes were significantly down-regulated (p < 0.05). Quantitative reverse transcription polymerase chain reaction analysis verified the transcriptome results of RNA sequencing. Gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that the 3 pathways of RNA polymerase, steroid biosynthesis, and ribosome biogenesis in eukaryotes are closely related to the antifungal mechanism of KTZ. Conclusions: The results indicated that KTZ may change cell membrane permeability, destroy the cell wall, and inhibit mitosis and transcriptional regulation through CYP51, SQL, ERG6, ATM, ABCB1, SC, KER33, RPA1, and RNP genes in the 3 pathways. This study provides a new theoretical basis for the effective control of M. canis infection and the effect of KTZ on fungi.