• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.24-30
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• 2012

Naehanyuchae and Youngsanyuchae were originated from the cross between "Erra(female)" and "Tower(male)". The cultivars were made at Bioenergy Crop Research Center, National Institute of crop science, Rural Development Administration, Korea in order to develop the high cold tolerant and yielding rapeseed variety with non-erucic acid and non-glucosinolate as good seed quality indicator. The superiorities of these varieties have been shown through the regional performance trials and demonstration farmers fields. Naehanyuchae and Youngsanyuchae had good agronomic characteristics related to yield potential such as many valuable branches and pods, and long pod length. The seeds of these new varieties composed of the 80% of oleic acid and linoleic acid without erucic acid, and glucosinolate was not extracted in the oil cake. The average grain yields increased by the from 16% to 22% according to the regional performance trials compared to check variety.

• Journal of Plant Biotechnology
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• v.29 no.1
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• pp.7-13
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• 2002

Superoxide dismutase (SOD) plays an important role in cellular defense against oxidative stress in plants. We have developed transgenic tomato plants overexpressing a cassava SOD in fruits. Three transgenic tomato plants (one from cv. Pink forcer and two from cv. Koko) using a new vector system, ASOp :: . mSOD1/pBI101, harboring ascorbate oxidase promoter (ASOp) expressing dominantly in cucumber fruits, CuZnSOD cDNA (mSOD1) isolated from cultured cells of cassava, and nptll gene as a selectable marker were successfully developed. SOD specific activity (units/mg protein) in transgenic fruits of both cultivars was increased with maturation of the fruits. SOD specific activity of well-mature fruits in transgenic Pink forcer and Koko showed approximately 1.6 and 2.2 times higher than control fruits, respectively. The strength of SOD isoenzyme bands well reflected the SOD activity during the fruit maturation. These results suggested that SOD gene was properly introduced into tomato fruits in a fruit-dominant expression manner by ASO promoter.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.278-285
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• 2008

An efficient transformation method for soybean [Glycine max (L.) Merr.] using meristematic tissues of germinating seeds has been established. The embryonic axes were excised from germinating seeds of Korean soybean cultivar, Iksannamulkong and 0.5-2 cm long segment containing meristematic tissues were prepared by cutting hypocotyl region. The explants were inoculated with Agrobacterium tumefaciens strain LBA4404 harboring a binary vector with the bar gene as a selectable marker gene and a ${\beta}-glucuronidase$ (GUSINT) reporter gene, and then co-cultured for 7 days on co-cultivation medium (CCM). The meristematic tissues were cultured on shoot induction medium (SIMP6) supplemented with 0.4 mg/l $N_6-benzylaminopurine$ (BAP) and 0.1 mg/l indolebutyric acid (IBA) in the presence of 6 mg/l L-phosphinotricin (PPT) for 2 weeks and the surviving explants were transferred to shoot elongation medium (SEMP6). Transformation was confirmed by Southern blot analysis and the transformation efficiencies ranged from 1.48 to 2.07%. The new modified transformation method was successfully implemented for obtaining several transgenic lines with SMV-CP gene. It is expected that this method could efficiently be used for the transformation of recalcitrant soybean cultivars.

• FLOWER RESEARCH JOURNAL
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• v.16 no.2
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• pp.118-123
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• 2008

For effective production of interspecific hybrids of Lilium and valuable new cultivars, we used Lilium Asiatic hybrid 'Tiny Ghost' as the female parent, and Oriental group, and Longiflorum group and Martagon as the male parents. Percentage pollen germination in Oriental hybrid 'Sorbonne' was the highest with 64%. As results of using normal style pollination and cut style pollination methods, all seed sets were formed in normal style pollinated treatment, except in 'Aktiva'. Pollen germination percent of Longiflorum hybrid 'Norina' was higher than that 'Gelria'. However, all crossings using 'Norina' failed to set seeds. Corresponding fertilization ratio was different from genetic compatibility, even in the same group. From the crossing using 'Aktiva' as the male parent one bulb was obtained derived from one embryo sac. And using 'Sorbonne' as the male parent, one plantlet was obtained after four ovule cultures. Crossing using 'Gelria' as the male parent five embryos, 15 embryo sacs, and seven ovules per one ovary were obtained. Among them, 18 plantlets germinated, with the germination percent of 66.7%. Crossings using L. hansonii, Martagon group, as the male parent resulted in 40% germination from five ovules.

• FLOWER RESEARCH JOURNAL
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• v.19 no.4
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• pp.264-268
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• 2011

'Duo', a late-flowering white gladiolus with pink core, was released by the National Institute of Horticultural & Herbal Science (NIHHS) in 2000. The selection was derived from the cross between the progenies of the early flowering dark pink 'True Love' and bright pink domestic cultivars 'Madame Valdiek'. The breeding was conducted in 1996 and selected finally in 2000 after four years (1997 to 2000) of experimentation. 'Duo' is a cut flower with bright white color petals and Pink core which exhibited late flowering and vigor without stem bending. It can be cultivated in areas or regions with strong winds during summer. 'Duo' has a very firm growth form and sturdy branches which do not bend when planted in the open fields. Likewise, the plant showed high tolerance to flooding and resistance, hence, could grow vigorously in open areas. It is a fast- growing plant which exhibits high corm production rate. 'Duo' had a 12.2 cm wide flower and 150 cm plant height. During summer, the average number of days to flowering was recorded at 75 days of 'Duo'. Top dressing must be conducted once or twice during corm production to enhance corm quality and quantity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.3
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• pp.185-192
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• 2019

Early-maturing rice type is cultivated to produce rice before the Korean Thanksgiving Day. We investigated the flowering and ripening characteristics of major breeding lines in early maturing rice type. In Jeonju, field growth days (FGD) from transplanting to heading changed about 3.3 days by 10 days of transplanting days and about 2.5 days by the change of mean temperature during growth period. As the temperature during ripening stage, 1000 brown rice weight was changed 0.2 g, the rice quality of brown and milled rice was changed 5% and 6.5%, respectively. Baegilmi and Kittake showed early heading habit suitable for harvesting before Korean Thanksgiving. Joun and Pecos showed good ripening characteristics under high temperature during the ripening stage. We expect that these characteristics might be useful for breeding new rice cultivars for harvesting before Korean Thanksgiving.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.534-538
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• 2009

Japanese apricot (Prunus mume) "Okboseok" was released from a cross between "Nanko" and "Yoseibai" in 1993 at the Pear Research Station of National Institute of Horticultural and Herbal Science, Rural Development Administration. It was preliminarily selected in 2001 and named in 2006. It produces high yield with a flesh about average 88.3% of fruit, although the fruit size is small (approximately 14.0g). Horticultural and physiological harvest time of this cultivar is mid and late June respectively. It has single flowers with pink petal color and its flowering time is about 4 days later than that of "Nanko". Its fruit is tolerant to bacterial shot hole (Xanthomonas arboricola pv. Pruni) and pear scab (Venturia nashicola). "Okboseok" has abundant pollen grains (about 96 mg/100 flowers), thus it could be a pollinizer. To obtain stable yields, however, its compatibility with major cultivars is needed to be confirmed as pollinizer cultivar. "Okboseok" is recommended for being preserved in sugar and liquor.

• The Journal of the Convergence on Culture Technology
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• v.5 no.1
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• pp.413-416
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• 2019

Alstroemeria (Alstroemeriaceae) is one of the most important cut flowers in international market. Especially, characteristics like long vase-life, various colors, tolerance to low temperature and a low energy requirement during cultivation have stimulated this success. Because of its characteristics such as low multiplication rates, time-consuming process and high risk of carrying viral disease, in vitro propagation techniques based on rhizome meristems culture have been developing nowadays. The callus induction has various cultivation sites compared with the direct plant generation method, and if the callus is maintained well, the plant differentiation can be performed simultaneously while maintaining the callus, so that it can be used for mass proliferation. In this study, we tested various hormones and cultivars for efficient callus induction. As a result of culturing between the nodes and the internodes, the callus began to be formed after 8 weeks, and the calli incidence in the nodes was higher than that between the internodes. Also, in the comparison of 2,4-D and picloram, the callus incidence rate was up to 2 times higher in the medium treated with 2,4-D. Using these results, it is thought that it will help establish the system of mass propagation system of Alstroemeria and cultivate new varieties.

• Journal of Life Science
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• v.33 no.8
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• pp.632-638
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• 2023

A soybean cultivar with a green seed coat and cotyledon contains high levels of lutein, which is beneficial for eye health. Plus, antinutritional components such as lipoxygenase, Kunitz trypsin inhibitor (KTI), lectin and stachyose exist in the mature seed. The genetic elimination of these antinutritional factors is a necessary step in green soybean breeding. This research was conducted to improve a new green soybean line with the green cotyledon and tetra null genotype (lox1lox2lox3tilers2) in terms of lipoxygenase, KTI, lectin and stachyose. We used five germplasms to develop a breeding population. A total of 69 F₂ seeds were obtained from the cross of parent 1 and parent 2, and from those, 21 F₂ seeds were selected that had the green seed coat color, and which were free of lectin protein. Next, four F₂ plants with the green seed coat and tetra null genotype were selected from the breeding population derived from four genotypes. The absence of lipoxygenase, KTI and lectin proteins was confirmed in the F₅ strain. The breeding line has a green seed coat, green cotyledon and white hilum color. The 100-seed weight and stachyose content for the breeding line were 30.7 g and 2.40 g/kg, respectively. The line selected in this study could be used as a cultivar or parent to improve colored soybean cultivars through the removal of antinutritional components such as lip- oxygenase, KTI, lectin and stachyose.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.1
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• pp.1-9
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• 2022

This study was carried out to compare the DMY (dry matter yield) of IRG (Italian ryegrass) in the southern coastal regions of Korea due to seasonal climate scenarios such as the Kaul-Changma (late monsoon) in autumn, extreme winter cold, and drought in the next spring. The IRG data (n = 203) were collected from various Reports for Collaborative Research Program to Develop New Cultivars of Summer Crops in Jeju, 203 Namwon, and Yeungam from the Rural Development Administration - (en DASH). In order to define the seasonal climate scenarios, climate variables including temperature, humidity, wind, sunshine were used by collected from the Korean Meteorological Administration. The discriminant analysis based on 5% significance level was performed to distinguish normal and abnormal climate scenarios. Furthermore, the DMY comparison was simulated based on the information of sample distribution of IRG. As a result, in the southern coastal regions, only the impact of next spring drought on DMY of IRG was critical. Although the severe winter cold was clearly classified from the normal, there was no difference in DMY. Thus, the DMY comparison was simulated only for the next spring drought. Under the yield comparison simulation, DMY (kg/ha) in the normal and drought was 14,743.83 and 12,707.97 respectively. It implies that the expected damage caused by the spring drought was about 2,000 kg/ha. Furthermore, the predicted DMY of spring drought was wider and slower than that of normal, indicating on high variability. This study is meaningful in confirming the predictive DMY damage and its possibility by spring drought for IRG via statistical simulation considering seasonal climate scenarios.