Browse > Article
http://dx.doi.org/10.5010/JPB.2002.29.1.007

Transgenic Tomato Plants That Overexpress Superoxide Dismutase in Fruits  

Park, Eun-Jeong (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Lee, Haeng-Soon (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Kwon, Suk-Yoon (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Choi, Kwan-Sam (Department of Agricultural Biology, Chungnam National University)
Kwak, Sang-Soo (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Journal of Plant Biotechnology / v.29, no.1, 2002 , pp. 7-13 More about this Journal
Abstract
Superoxide dismutase (SOD) plays an important role in cellular defense against oxidative stress in plants. We have developed transgenic tomato plants overexpressing a cassava SOD in fruits. Three transgenic tomato plants (one from cv. Pink forcer and two from cv. Koko) using a new vector system, ASOp :: . mSOD1/pBI101, harboring ascorbate oxidase promoter (ASOp) expressing dominantly in cucumber fruits, CuZnSOD cDNA (mSOD1) isolated from cultured cells of cassava, and nptll gene as a selectable marker were successfully developed. SOD specific activity (units/mg protein) in transgenic fruits of both cultivars was increased with maturation of the fruits. SOD specific activity of well-mature fruits in transgenic Pink forcer and Koko showed approximately 1.6 and 2.2 times higher than control fruits, respectively. The strength of SOD isoenzyme bands well reflected the SOD activity during the fruit maturation. These results suggested that SOD gene was properly introduced into tomato fruits in a fruit-dominant expression manner by ASO promoter.
Keywords
Ascorbate oxidase promoter; Lycopersicon esculentum; oxidative stress; plant bioreactor; superoxide dismutase;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Cohen G (1988) Oxygen radicals and Parkinson,s disease. In Oxygen Radicals and Tissue Injury' (Halliwell, B., ed). Federation of American Societies for Expermental Biology, Bethesda, MD:130-135
2 Fridovich I (1982) Measuring the activity of superoxide dismutates: an embarrassment of riches. In LW Oberly, ed, Superoxide Dismutase, Vol 1. CRC Press, Boca Raton, FL, pp 69-77
3 Gambley RL, Dodd WA (1991) The influence of cotyledons axillary and adventitious shoot production from cotyledonary nodes of Cucumis sativus L (cucumber). J Exp Bot 32:1131-1135
4 Goddijin OJM, Pen J (1995) Plants as bioreactors. TIBTECH 13:379-387   DOI   ScienceOn
5 McKersie BD, Chen Y, De Beus M, Bowley SR, Bowler C (1993) Superoxide dismutase engances tolerance of freezing stress in transgenic alfalfa (Medicago sativa L.). Plant Physiol 103:1155-1163   DOI   ScienceOn
6 Michelmore R (1996) Big news for plant transformation. Nature Biotechnol. 14 (13):1653-4
7 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497   DOI
8 Allen RD (1995) Dissection of oxidative stress tolerance using transgenic plants. Plant Physiol 107:1049-1054   DOI
9 Alscher RQ, Hess JL (1993) Antioxidants in Higher Plants. CRC Press, Boca Raton, pp 1-174
10 Asemota HN (1995) A fast, simple, and efficient miniscale method for the preparation of DNA from tissues of yam (Dioscorea spp.). Plant Mol Biol Rep 1: 19-21   DOI
11 Bannister JV, Bannister WH, Rotilio G (1987) Aspects of the structure, function and applications of superoxide dismutase. CRC Crit Rev Biochem 22: 111-180   DOI
12 Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276-287   DOI   ScienceOn
13 Inze D, Van Montagu M (1995) Oxidative stress in plants. Curr Opin Biotechnol 6:153-158   DOI   ScienceOn
14 Bradford MM (1976) A rapid and sensitive method for the quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248-254   DOI   ScienceOn
15 Haber F, Weiss J (1934) The catalytic decomposition of hydrogen peroxide by iron salts. Proc R Soc Lond A 147:332-351   DOI
16 Hanson B, Engler D, Moy Y, Newman B, Ralston E, Gutterson N. (1999) A simple method to enrich an Agrobacterium-trans-formed population for plants containing only T-DNA sequences. Plant J 19:727-34   DOI   ScienceOn
17 Kim JH, Oh SY, Lee HS, Jo MH, Lee EM, Woo IS, Kwak SS (1998) Expression of pea superoxide dismutase gene in transgenic cucumber (Cucumis sativus L.) plants. Korean J. Plant Tissue Culture 25:201-206
18 Lee HS, Kim KY, You SH, Kwon SY, Kwak SS (1999) Molecular characterization and expression of a cDNA encoding copper/zinc superoxide dismutase from cultured cells of cassava (Manihot escuIenta Crantz). Mol Gen Genet 262:807-814
19 Mason HS, Arntzen CJ (1995) Transgenic plants as vaccine production systems. TIBTECH 13: 388-392   DOI   ScienceOn
20 McCord JM, Fridovich I (1969) Superoxide dismutase. An enzymatic function for erythrocuprein (Hemocuprein). J Biol Chem 244:6049-6055
21 Sen Gupta A, Webb RP, Holaday AS, Allen RD (1993) Overexpressin of superoxide dismutases protects plants from oxidative stress. Plant Physiol 103:1067-1073   DOI
22 Ohkawa J, Ohya T, Ito T, Nozawa H, Nishi Y, Okada N, Yoshida K, Takano M, Shinmyo A (1994) Structure of the genomic DNA encoding cucumber ascorbate oxidase and its expreesion in transgenic plants. Plant Cell Rep 13:481-488
23 Perl A, Perl-Treves R, Galili S, Aviv D, Shalgi E, Malkin S, Galun E (1993) Enhanced oxidative-stress defence in transgenic potato expression tomato Cu, Zn superoxide dismutases. Theor Appl Genet 85:568-576
24 Pfitzner AJ (1998) Transformation of tomato. Methods Mol Biol 81:359-63
25 Tepperman JM, Dunsmuir P (1990) Transformed plants with elevated levels of chloroplastic SOD are not more resistant to superoxide toxicity. Plant Mol Biol 14:501-511   DOI   ScienceOn