• Maxillofacial Plastic and Reconstructive Surgery
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• v.33 no.1
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• pp.19-25
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• 2011

Purpose: Addition of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) to grafting material has become widely accepted additively for bone regeneration because it can raise high expectations on it's clinical potential. The aim of this study was to evaluate the efficacy of PRP and PRF on early bone regeneration of rabbits when used in combination with beta tricalcium phosphate. Methods: In eight rabbits, the calvarium was exposed and the two marrows were penetrated. After then these artificial bone defects were augmented with ${\beta}$-TCP or ${\beta}$-TCP with PRP or ${\beta}$-TCP with PRF and covered. The animals were sacrificed after four and eight weeks. Histologic findings were observed under the light-microscope and histomorphometric analysis was performed by measuring calcified area of new bone formation within the CSD. Results: They demonstrated that new bone formation tended to be produced along the outline of graft materials. More amounts of newly bone was regenerated in ${\beta}$-TCP only and in combination of${\beta}$-TCP with PRF and it was statistically significant. In contrast, there was no significant difference between nothing apply and ${\beta}$-TCP with PRP groups in the relative amounts of newly mineralized bone. Conclusion: Within the limitation of this study, it can be concluded that PRF in combination with ${\beta}$-TCP showed a positive effect on bone regeneration and statistically it was significant.

• The International Journal of Costume Culture
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• v.8 no.1
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• pp.32-42
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• 2005

This paper aims to propose fashion designs based on the application of art flower technique to translucent clothing material. For study method, firstly I looked into art flower applications occurred in modern trend since 2000 as well as theoretical research on art flower and transparency expressed as artificial beauty. Second, I attempt to express transparency in a variety of unique ways by using the art flower technique in producing clothes. Third, I utilized transparent flower with translucent clothing material and tried to suggest fashion design attempting mixture of new materials. As a result, firstly transparent image and material are well fitted in with modern trend and especially it was very suitable for expressing feminine beauty. Second, transparency was the element to suggest creative formative world in fashion design in regard to flower's beauty, various shapes and colors and to provide infinite materials as design motive. Third, the combination of knit clothing and plastic art flower displayed a new form of material combination. Especially as translucent material is fitted with trend such as function, lightness and variableness in modern times of the $21^{st}$ century, it presents beautiful combination with transparent flower. Fourth, Silk flower technique is variously used in art flower techniques. Various possibility ranges are presented such as flower was recreated with artificial image by silk flower technique to be newly expressed and various materials like aesthete film can be also expressed with silk flower technique.

• The Research Journal of the Costume Culture
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• v.17 no.6
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• pp.972-980
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• 2009

This study was aimed to develop ume flower image into a competitive fashion culture product image by reinterpreting the image in modern terms, manufacturing patterns and applying them to various items. In terms of method, ume flower petal was used as a motive and developed into a pattern, using Adobe Illustrator 10, a computer design program. Based on the symbolic image and realist form of ume flower, three new basic motives of new figurative image were set using form omission, simplification, overlapping, repetition and graphic elements. Each motive developed transformed patterns through the change, transformation, combination of colors. The repetitive unit of each motive set expressed geometrical patterns and combination of flower patterns using pattern repetition and $45^{\circ}$ repetition technique in combination with the check arrangement using quadrangle, and set the direction of design that would fit for each item of fashion culture products. Also, consistency and practicality were sought in the goods planning composition of each item by applying motive pattern results to the fashion culture goods, such as neckties, scarves, T-shirts that can be consumed in everyday life. It seems that more creative culture goods including ume flowers will be developed by seeing our own cultural elements as well as flower patterns like ume flower with modern trends.

• Asian Journal of Atmospheric Environment
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• v.2 no.2
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• pp.75-80
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• 2008

A new-type apparatus decomposing volatile organic compounds (VOCs) using a combination system of an electrical exothermic SiC honeycomb and a catalytic filter was developed. This linear combination system is very useful to the catalytic decomposition of VOCs, because the gas involving VOCs is well heated in the SiC honeycomb and then flows into the catalytic filter. In the proposed apparatus, the outlet gas temperatures of SiC honeycomb maintained at ca. $300^{\circ}C$ after 5 min from the starting of applying electric current, and sufficient for the catalytic degradation of VOC components, i.e. toluene, isopropanol, methyl ethyl ketone and ethyl acetate. The average decomposition rate of total VOCs exhausted from a printing factory was 85% using pt catalyst at SV=19,000 in this system.

• Biomaterials Research
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• v.22 no.4
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• pp.311-318
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• 2018

Background: Tissue regeneration includes delivering specific types of cells or cell products to injured tissues or organs for restoration of tissue and organ function. Stem cell therapy has drawn considerable attention since transplantation of stem cells can overcome the limitations of autologous transplantation of patient's tissues; however, it is not perfect for treating diseases. To overcome the hurdles associated with stem cell therapy, tissue engineering techniques have been developed. Development of stem cell technology in combination with tissue engineering has opened new ways of producing engineered tissue substitutes. Several studies have shown that this combination of tissue engineering and stem cell technologies enhances cell viability, differentiation, and therapeutic efficacy of transplanted stem cells. Main body: Stem cells that can be used for tissue regeneration include mesenchymal stem cells, embryonic stem cells, and induced pluripotent stem cells. Transplantation of stem cells alone into injured tissues exhibited low therapeutic efficacy due to poor viability and diminished regenerative activity of transplanted cells. In this review, we will discuss the progress of biomedical engineering, including scaffolds, biomaterials, and tissue engineering techniques to overcome the low therapeutic efficacy of stem cells and to treat human diseases. Conclusion: The combination of stem cell and tissue engineering techniques overcomes the limitations of stem cells in therapy of human diseases, and presents a new path toward regeneration of injured tissues.

• Structural Engineering and Mechanics
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• v.82 no.5
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• pp.667-678
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• 2022

Moment-resisting frames (MRFs) are among the most conventional steel structures for mid-rise buildings in many earthquake-prone cities. Here, a simplified performance-based methodology is proposed for the seismic collapse capacity assessment of these buildings. This method employs a novel multi-mode pushover analysis to determine the engineering demand parameters (EDPs) of the regular steel MRFs up to the collapse prevention (CP) performance level. The modal combination coefficients used in the proposed pushover analysis, are obtained from two metaheuristic optimization algorithms and a fitting procedure. The design variables for the optimization process are the inter-story drift ratio profiles resulting from the multi-mode pushover analyses, and the objective values are the outcomes of the incremental dynamic analysis (IDA). Here, the collapse capacity of the structures is assessed in three to five steps, using a modified IDA procedure. A series of regular mid-rise steel MRFs are selected and analyzed to calculate the modal combination coefficients and to validate the proposed approach. The new methodology is verified against the current existing approaches. This comparison shows that the suggested method more accurately evaluates the EDPs and the collapse capacity of the regular MRFs in a robust and easy to implement way.

• Annals of Surgical Treatment and Research
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• v.95 no.5
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• pp.240-248
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• 2018

Purpose: This study aimed to validate the synergistic effect of ABT-737 on docetaxel using MDA-MB-231, a triple negative breast cancer (TNBC) cell line overexpressing B-cell lymphoma-2 (Bcl-2). Methods: Western blot analysis was performed to assess expression levels of Bcl-2 family proteins and caspase-related molecules. Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle distribution was determined by flow cytometry analysis. Benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone (z-VAD-fmk) was used for pretreatment to assess the role of caspases. Results: Cell viability of MDA-MB-231 after combination treatment with ABT-737 and docetaxel was significantly lower than that after docetaxel or ABT-737 monotherapy based on MTT assay (both P < 0.001), with a combination index of 0.41. The proportion of sub-G1 population after combination treatment was significantly higher than that after docetaxel or ABT-737 monotherapy (P = 0.001, P = 0.003, respectively). Pretreatment with z-VAD-fmk completely restored cell viability of MDA-MB-231 from apoptotic cell death induced by combination therapy (P = 0.001). Although pro-caspase-8 or Bid did not show significant change in expression level, pro-casepase-9 showed significantly decreased expression after combination treatment. Cleaved caspase-3 showed increased expression while poly (ADP-ribose) polymerase cleavage was induced after combination treatment. However, hypoxia-inducible factor 1-alpha and aldehyde dehydrogenase 1 totally lost their expression after combination treatment. Conclusion: Combination of ABT-737 with docetaxel elicits synergistic therapeutic effect on MDA-MB-231, a TNBC cell line overexpressing Bcl-2, mainly by activating the intrinsic pathway of apoptosis. Therefore, adjunct of ABT-737 to docetaxel might be a new therapeutic option to overcome docetaxel resistance of TNBCs overexpressing Bcl-2.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.4
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• pp.2307-2310
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• 2013

Curcumin previously was proven to inhibit angiogenesis and display potent antitumor activity in vivo and in vitro. In the present study, we investigated whether a combination curcumin with hyperthermia would have a synergistic antitumor effect in the LL/2 model. The results indicated that combination therapy significantly inhibited cell proliferation of MS-1 and LL/2 in vitro. LL/2 experiment model also demonstrated that the combination therapy inhibited tumor growth and prolonged the life span in vivo. Furthermore, combination therapy reduced angiogenesis and increased tumor apoptosis. Our findings suggest that the combination therapy exerted synergistic antitumor effects, providing a new perspective fpr clinical tumor therapy.

• Knowledge Management Research
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• v.2 no.1
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• pp.65-81
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• 2001

The aim of this study is classify knowledge work style management for knowledge management. It is based on the knowledge creation model of Nonaka by subdividing types of knowledge workers. It was designed to create a model for application to the actual environment of management. Nonaka suggested the process of socialization, externalization, combination, internalization that the knowledge of a person creates new knowledge through the interaction of implicit knowledge and explicit knowledge. This research demonstrated that knowledge worker of 16 forms by applying SECI model to the main function and the subordinate functions again. This study aims at achieving a higher outcome by applying the ability of existing knowledge worker to subdivided expert field efficiently. Suggested styles of knowledge worker in this research are classified into craftsman style, pragmatic style, combination style, developed style knowledge worker who creates knowledge by selecting socialization as the function and again by selecting externalization combination, internalization as subordinate functions. And they were classified into creation style, insight style, strategy style according to practical application worker and function which is selecting externalization as the main function and socialization as the subordinate functions. They were classified into future style, innovation style, analysis style, judgement style knowledge worker who are selecting combination as the main function and experiment style, intuition style, research style, learning style worker who are selecting internalization as the main function. They suggested the characteristics and cases of each type.

• BMB Reports
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• v.55 no.7
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• pp.336-341
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• 2022

Narrowing of arteries supplying blood to the limbs provokes critical hindlimb ischemia (CLI). Although CLI results in irreversible sequelae, such as amputation, few therapeutic options induce the formation of new functional blood vessels. Based on the proangiogenic potentials of stem cells, in this study, it was examined whether a combination of dental pulp stem cells (DPSCs) and human umbilical vein endothelial cells (HUVECs) could result in enhanced therapeutic effects of stem cells for CLI compared with those of DPSCs or HUVECs alone. The DPSCs+ HUVECs combination therapy resulted in significantly higher blood flow and lower ischemia damage than DPSCs or HUVECs alone. The improved therapeutic effects in the DPSCs+ HUVECs group were accompanied by a significantly higher number of microvessels in the ischemic tissue than in the other groups. In vitro proliferation and tube formation assay showed that VEGF in the conditioned media of DPSCs induced proliferation and vessel-like tube formation of HUVECs. Altogether, our results demonstrated that the combination of DPSCs and HUVECs had significantly better therapeutic effects on CLI via VEGF-mediated crosstalk. This combinational strategy could be used to develop novel clinical protocols for CLI proangiogenic regenerative treatments.