• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3669-3676
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• 2013

Faithful transmission of genetic information depends on accurate chromosome segregation as cells exit from mitosis, and errors in chromosomal segregation are catastrophic and may lead to aneuploidy which is the hallmark of cancer. In eukaryotes, an elaborate molecular control system ensures proper orchestration of events at mitotic exit. Phosphorylation of specific tyrosyl residues is a major control mechanism for cellular proliferation and the activities of protein tyrosine kinases and phosphatases must be integrated. Although mitotic kinases are well characterized, phosphatases involved in mitosis remain largely elusive. Here we identify a novel variant of mouse protein tyrosine phosphatase containing domain 1 (Ptpcd1), that we named Ptpcd2. Ptpcd1 is a Cdc14 related centrosomal phosphatase. Our newly identified Ptpcd2 shared a significant homology to yeast Cdc14p (34.1%) and other Cdc14 family of phosphatases. By subcellular fractionation Ptpcd2 was found to be enriched in the cytoplasm and nuclear pellets with catalytic phosphatase activity. By means of immunofluorescence, Ptpcd2 was spatiotemporally regulated in a cell cycle dependent manner with cytoplasmic abundance during mitosis, followed by nuclear localization during interphase. Overexpression of Ptpcd2 induced mitotic exit with decreased levels of some mitotic markers. Moreover, Ptpcd2 failed to colocalize with the centrosomal marker ${\gamma}$-tubulin, suggesting it as a non-centrosomal protein. Taken together, Ptpcd2 phosphatase appears a non-centrosomal variant of Ptpcd1 with probable mitotic functions. The identification of this new phosphatase suggests the existence of an interacting phosphatase network that controls mammalian mitosis and provides new drug targets for anticancer modalities.

• 열처리공학회지
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• 제31권6호
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• pp.290-299
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• 2018

The microstructure of a newly developed alloy tool steel (KV1) for casting cold-work press dies was analyzed using advanced scanning electron microscopy. Its mechanical properties and durability in use as piercing inserts were tested. It contained a continuous network structure which originated from the micro-segregation during solidification and was composed of retained austenite and primary carbides. However, after quenching and tempering, its continuity was destroyed due to the decomposition of austenite. The discontinuous spatial distribution and the smaller amount of primary carbide in the network presented KV1 enhanced ductility compared to the common alloy (HK700). The reduced C and Cr, the addition of V resulted in a small amount of primary carbides which primarily consisted of MC, as well as fine and uniform dispersion of precipitates. Owing to these features, KV1 exhibited delayed initiation of chipping when used for piercing inserts.

• 지역연구
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• 제35권4호
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• pp.47-60
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• 2019

본 연구의 목적은 수도권 임금근로자를 대상으로 직종 성별 분리 및 숙련 특성이 임금 및 직무만족도의 성별 격차에 미치는 영향을 분석하는 데 있다. 노동시장 내 성별 격차를 다룬 선행연구들은 개인 수준에만 국한하여 분석을 수행하는 것에 그쳤다는 한계를 가진다. 본 연구는 위계적 선형모형을 활용하여 개인과 직종 두 층위의 변수를 포괄하여 분석을 수행함으로써 이와 같은 한계를 극복하고자 하였다. 실증분석을 위해 한국노동패널조사 20차년도 자료와 한국직업정보시스템의 직종별 업무수행능력 중요도 및 수준 자료가 활용되었으며 인지적 숙련, 기술적 숙련, 신체적 숙련을 도출하였다. 분석결과에 따르면, 수도권 여성 임금근로자는 임금과 직무만족도 양쪽 측면에서 남성 임금근로자에 비해 평균적으로 낮은 수준을 보였다. 이와 같은 성별 격차는 인지적 숙련을 높은 수준으로 요구하는 직종에서는 보다 완화되는 한편, 직종 성별 분리의 정도가 높은 직종에서는 심화되는 것으로 나타났다. 본 연구는 직종 성별 분리와 숙련 특성에 따른 임금 및 직무만족도의 성별 격차의 관계를 분석함으로써 성별 격차 해소를 목적으로 하는 수도권 노동시장 내 정책의제에 대한 시사점을 이끌어내고자 하였다.

• 한국세라믹학회지
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• 제31권9호
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• pp.1044-1052
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• 1994

Tape casting technique was successfully applied to produce porous ceramics and multi-layered ceramics containing porous layers, where spherical hollow polymer particles were introduced as pore precursors. In the presence of extreme differences in density and size between Al2O3 and pore precursor particles, hindered settling was effective in preventing segregation of component particles and packing behavior of mixed powders was improved through bimodal packing. There were two transitions in packing behavior of mixed powders. The first transition took place at 40~50 vol% pore precursor addition, where majority of pores changed from close to open pore state. The other transition occured at 60~70 vol% pore precursor addition, where pore precursor particles formed a continuous network structure.

• Advances in concrete construction
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• 제15권2호
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• pp.85-96
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• 2023

The characteristics of self-compacting concrete (SCC) made with fly ash and reinforced with polyester fibers were investigated in this research. Polyester fibers of 12 mm long and 15 micrometer diameters were utilized in M40 grade SCC mixtures at five different volume fractions 0.025%, 0.05%, 0.075%, 0.1%, 0.3% as a fiber reinforcement. To understand the influence of polyester fibers on passing ability, flowability, segregate resistance the J ring, L box, V funnel, slump flow and U box tests were performed. Polyester fibers have a direct influence, with a maximum of 0.075% polyester fibers producing excellent characteristics. ANN models were constructed using the testing data as inputs to anticipate the fresh and hardened characteristics as targeted outputs. The research revealed that R² values ranging from 0.900 to 0.997 appears to be a good correlation. The performance of ANN models and regression models for predicting the new characteristics of SCC is also evaluated.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제10권9호
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• pp.4529-4548
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• 2016

DDoS attacks have had a devastating effect on the Internet, which can cause millions of dollars of damage within hours or even minutes. In this paper we propose a practical dynamic defense approach that overcomes the shortage of static defense mechanisms. Our approach employs a group of SDN-based proxy switches to relay data flow between users and servers. By substituting backup proxy switches for attacked ones and reassigning suspect users onto the new proxy switches, innocent users are isolated and saved from malicious attackers through a sequence of remapping process. In order to improve the speed of attacker segregation, we have designed and implemented an efficient greedy algorithm which has been demonstrated to have little influence on legitimate traffic. Simulations, which were then performed with the open source controller Ryu, show that our approach is effective in alleviating DDoS attacks and quarantining the attackers by numerable remapping process. The simulations also demonstrate that our dynamic defense imposes little effect on legitimate users, and the overhead introduced by remapping procedure is acceptable.

• 한국세라믹학회지
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• 제26권2호
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• pp.210-220
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• 1989

The properties of the powder of Al2O3-15v/o ZrO2(＋3m/o Y2O3) system prepared by co-precipitation method at the pH values of 7, 9, 10 and 11 were investigated. Al2(SO4)3.18H2O, ZrOCl2.8H2O and YCl3.6H2O were used as starting materials and NH4OH as a precipitation agent. Zirconium hydroxide decreased the specific surface area of aluminum hydroxide of AlOOH type, while increased the specific surface area of aluminum hydroxide of Al(OH)3 type, and formed co-network structure of Al-O-Zr type with the aluminum hydroxides. The rate of transition to $\alpha$-Al2O3 from co-precipitated materials occurred in the order of 7≒10, 9 and 11 of pH values. Al2O3 and ZrO2 interacted to bring about coupled grain growth, and the growth of ZrO2 crystallite size rapidly occurred within $\theta$-Al2O3 matrix. Segregation did not occur in the system Al2O3-15v/o ZrO2(＋3m/o Y2O3) and Y2O3 acted as a stabilizer to ZrO2. The lattice strain of tetragonal ZrO2 was increased by the constraint effect of Al2O3 matrix.

• 한국지역지리학회지
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• 제3권2호
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• pp.209-225
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• 1997

본 연구는 대구시에 거주하며 경제적 사회적으로 안정된 지위에 있는 저명인사를 대상으로 사회지역 분석을 시도하였다. 이는 도시내의 불량주거 또는 저소득층의 주거에 관한 기존의 고찰과 더불어 의의가 있을 것이다. 대구에 거주하는 저명인사는 도심의 전통적인 주거지역인 구성곽(舊城郭)의 내외에 인접한 중구와 더불어, 토지구획정리사업이 진행된 지역중 수성구와 남구에 밀집되어 있다. 또한 쾌적한 주거환경을 위해 지정된 주거전용지역은 부유계층의 주거집단을 형성하면서 수성동, 만촌동, 대명동의 일부지역이 대구의 신흥 고급 주택지가 형성되는 계기가 된다. 오늘날 저명인사의 분포독성은 수성구에 가장 많이 거주하고, 남구, 중구의 순이다. 또 저명인사의 동별 절대수와 인구 1만명당 동별 분포 밀도가 상위 6위까지 모두 같은 동(범어4, 수성1가, 봉덕2, 대봉1, 봉덕3, 만촌2)들이 포함되어 있어 이들의 두 가지 순위는 대동소이한 것으로 나타났다. 이러한 결과는 저명인사들이 특정지역에 밀집하여 거주하고 있다는 것을 나타내고 있다. 지난 10년간 변화특성은 저명인사의 기존밀집지역인 중구와 남구는 감소하고, 수성구에 현저한 증가를 보인다. 이것은 지난 10년간 이 지역에 꾸준히 추진된 택지개발과 학교 등 공공용지의 이전에 따른 고급아파트 지역의 형성의 영향도 크며, 교통, 생활 편익 시설의 상태외에 주거입지결정 인자로서 주요한 위치를 차지하는 소위 교육여건이 유리한 학군이 저명인사의 분포에도 상당한 영향을 미친 것으로 판단된다. 이들 지역중에도 시간이 경과함에 따라 기존건축물의 노후화와 시가지의 외곽확대, 전원주택의 선호 경향 등 제반 여건의 변화에 따라 저명인사의 밀집현상은 다소 완화될 것이 전망된다.

• 한국인구학
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• 제27권1호
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• pp.1-30
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• 2004

이 연구의 목적은 노년기 젠더관계가 노인의 심리적 복지감(Psychological well-being)에 미치는 영향을 규명하는 것이다. 노인부부의 젠더관계를 규명하기 위해 성역할태도와 가사노동분담을 측정하였고 노인의 심리적 복지감은 우울도와 행복감으로 측정되었다. 연구의 대상은 부산광역시에 거주하는 배우자와 동거하고 자녀와는 동거하지 않으며, 부부 모두 직업에서 은퇴한, 65세 이상의 남녀노인 476명이다. 연구의 결과는 다음과 같다: 1) 여성노인이 남성노인에 비해 보다 평등적인 성역할태도를 지니고 있으며, 노인부부의 가사노동 수행에 있어 성별 분절현상이 나타난다. 2) 여성노인이 남성노인에 비해 성역할태도와 가사노동분담 수준이 불일치하는 경우가 더 많았으며, 남녀 노인 모두 성역할태도와 가사노동분담 수준이 일치할수록 심리적 복지감이 높았다. 3) 남녀노인의 성역할태도는 심리적 복지감에 영향을 미치지 못하는 것으로 나타났다. 4) 여성노인의 가사노동분담은 심리적 복지감에 별다른 영향을 미치지 못하고 있으며, 남성노인의 가사노동분담은 오히려 그들의 심리적 복지감을 낮게 한다.

• Animal cells and systems
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• 제9권2호
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• pp.87-94
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• 2005

Spindle position checkpoint monitors the orientation of mitotic spindle for proper segregation of replicated chromosomes into mother cell and the daughter, and prohibits mitotic exit when mitotic spindle is misaligned. BUB2 forms one of the key upstream element of spindle position checkpoint in budding yeast, but its functional homologues have not been identified in higher eukaryotes. Here, we analyzed the functions of two putative BUB2 homologues of C. elegans in the spindle orientation checkpoint. From the C. elegans genome database, we found that two open reading frames (ORFs), F35H12_2 and C33F10_2, showed high sequence homology with BUB2. We obtained the expressed sequence tag (EST) clones for F35H12_2 (yk221d4) and C33F10_2 (yk14e10) and verified the full cDNA for each ORF by sequencing and 5' RACE with SL1 primer. The functional complementation assays of yk221d4 and yk14e10 in ${\Delta}bub2$ of S. cerevisiae revealed that these putative BUB2 homologues of C. elegans could not replace the function of BUB2 in spindle position checkpoint and mitotic exit. Our attempt to document the component of spindle position checkpoint in metazoans using sequence homology was not successful. This suggests that structural information about its components might be required to identify functional homologues of the spindle position checkpoint in higher eukaryotes.